Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,968,0250CG100.0% 1594.6 / NA 402P426A (CCC→GCC) phaCclass II poly(R)‑hydroxyalkanoic acid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (222/180);  total (222/180)

Only 100 of 408 total aligned reads displayed.

GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172
                                                                                                                                                    |                                                                                                                                                   
gAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgcc                                                                                                                                                   >  2:9724988/1‑151 (MQ=255)
  actactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCt                                                                                                                                                 >  1:3335504/1‑151 (MQ=255)
   ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                >  1:6648157/1‑151 (MQ=255)
   ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                <  1:5873867/151‑1 (MQ=255)
   ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                >  2:9125128/1‑151 (MQ=255)
     actGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGa                                                                                                                                              <  1:2480165/151‑1 (MQ=255)
          gTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGtct                                                                                                                                         >  1:2994278/1‑151 (MQ=255)
            caacaaCTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTcc                                                                                                                                       >  2:10074922/1‑151 (MQ=255)
                 acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCAc                                                                                                                                  <  1:7204255/151‑1 (MQ=255)
                 acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCAc                                                                                                                                  <  2:7253990/151‑1 (MQ=255)
                 acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCAc                                                                                                                                  >  1:3955393/1‑151 (MQ=255)
                  cTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACg                                                                                                                                 <  2:9368067/151‑1 (MQ=255)
                   tACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGc                                                                                                                                >  2:1176225/1‑151 (MQ=255)
                   tACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGc                                                                                                                                >  2:6859320/1‑151 (MQ=255)
                    aCCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGcc                                                                                                                               >  1:4941075/1‑151 (MQ=255)
                    aCCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGcc                                                                                                                               >  2:8350212/1‑151 (MQ=255)
                         cTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCg                                                                                                                          >  2:10425548/1‑151 (MQ=255)
                          tCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGa                                                                                                                         <  1:9247391/151‑1 (MQ=255)
                          tCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGa                                                                                                                         >  2:860822/1‑151 (MQ=255)
                           cGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGAc                                                                                                                        >  1:4541316/1‑151 (MQ=255)
                            ggCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGAcc                                                                                                                       >  2:5480378/1‑151 (MQ=255)
                              cAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTg                                                                                                                     >  2:7020814/1‑151 (MQ=255)
                                 ccAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAg                                                                                                                  <  1:10560975/151‑1 (MQ=255)
                                  cAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGc                                                                                                                 >  2:5941119/1‑151 (MQ=255)
                                   cgccgccGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCGGGAGGTCTCCGGCACGCCCATCGACCTGAAGCa                                                                                                                <  1:5349526/150‑1 (MQ=255)
                                     ccgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAgg                                                                                                              >  2:7894918/1‑151 (MQ=255)
                                     ccgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAgg                                                                                                              <  1:1801607/151‑1 (MQ=255)
                                     ccgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAgg                                                                                                              >  2:4678036/1‑151 (MQ=255)
                                      cgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGt                                                                                                             >  2:3051810/1‑151 (MQ=255)
                                        ccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGa                                                                                                           <  2:3746045/151‑1 (MQ=255)
                                         cgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGAc                                                                                                          >  1:8358686/1‑151 (MQ=255)
                                            cGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                       <  1:9620568/151‑1 (MQ=255)
                                             gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                      >  2:5623373/1‑151 (MQ=255)
                                             gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                      >  2:669406/1‑151 (MQ=255)
                                              ttCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCg                                                                                                     >  2:2894812/1‑151 (MQ=255)
                                               tCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGa                                                                                                    >  1:6561502/1‑151 (MQ=255)
                                                cGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGAc                                                                                                   >  2:517946/1‑151 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  >  1:9476613/1‑151 (MQ=255)
                                                  aCATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACtt                                                                                                 >  2:766755/1‑151 (MQ=255)
                                                       ctctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACt                                                                                            <  2:1713352/151‑1 (MQ=255)
                                                        tctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACtg                                                                                           <  1:5468330/151‑1 (MQ=255)
                                                              ggAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGc                                                                                     >  1:7264503/1‑151 (MQ=255)
                                                               gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGcc                                                                                    >  2:4056124/1‑151 (MQ=255)
                                                                  caacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGt                                                                                 <  2:3913981/151‑1 (MQ=255)
                                                                    acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                               <  1:100211/151‑1 (MQ=255)
                                                                     cGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTg                                                                              <  2:1392682/151‑1 (MQ=255)
                                                                      gACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGa                                                                             >  1:1672378/1‑151 (MQ=255)
                                                                        caccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAAc                                                                           <  1:4166506/151‑1 (MQ=255)
                                                                        caccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAAc                                                                           <  2:2250998/151‑1 (MQ=255)
                                                                            acGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAcc                                                                       <  1:3145014/151‑1 (MQ=255)
                                                                              gcgcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACcac                                                                     >  1:2645500/1‑151 (MQ=255)
                                                                                gcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                   >  2:10751777/1‑151 (MQ=255)
                                                                                 ccTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATc                                                                  <  1:2043383/151‑1 (MQ=255)
                                                                                   tGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                                >  2:8940366/1‑151 (MQ=255)
                                                                                   tGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                                >  1:8164622/1‑151 (MQ=255)
                                                                                   tGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                                >  1:8898781/1‑151 (MQ=255)
                                                                                    gCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAcc                                                               <  1:5041404/151‑1 (MQ=255)
                                                                                     cccGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAccc                                                              <  1:7749724/151‑1 (MQ=255)
                                                                                       cgccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCCTCGACCTGAAGCTGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGTACGACCACATCAccccc                                                            >  1:6144408/1‑151 (MQ=255)
                                                                                       cgccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAccccc                                                            <  2:2516235/151‑1 (MQ=255)
                                                                                        gccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                           >  1:10462166/1‑151 (MQ=255)
                                                                                        gccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                           >  2:6480349/1‑151 (MQ=255)
                                                                                        gccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                           <  1:3507966/151‑1 (MQ=255)
                                                                                         ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                          <  2:2610887/151‑1 (MQ=255)
                                                                                         ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                          <  1:6925023/151‑1 (MQ=255)
                                                                                           gcgcTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTggg                                                        >  2:8001278/1‑151 (MQ=255)
                                                                                             gcTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                      >  1:10252535/1‑151 (MQ=255)
                                                                                                gCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCg                                                   <  1:1069763/151‑1 (MQ=255)
                                                                                                 cACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGt                                                  <  2:4549820/151‑1 (MQ=255)
                                                                                                   cGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGc                                                >  2:3316599/1‑151 (MQ=255)
                                                                                                   cGGAGAGGTCGTCGAACTGTTCACGATCAACCCGCTGAACCGACCCGGCGACCTGTAGGTCTCCGGCACGCCCATCGACCTTAAGCAGGTGAATAGCTACGTCTAATGTTTCGCCGGTCTTAACGACCACATCAACACCTGGGAGTCGTGc                                                <  2:9478399/151‑1 (MQ=255)
                                                                                                     gCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTa                                              >  2:4289379/1‑151 (MQ=255)
                                                                                                        aGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                           >  2:1564889/1‑151 (MQ=255)
                                                                                                           tcgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTc                                        <  1:7629436/151‑1 (MQ=255)
                                                                                                               cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGcc                                    <  2:3544995/151‑1 (MQ=255)
                                                                                                                     gTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTa                              <  2:9198535/151‑1 (MQ=255)
                                                                                                                        cAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTg                           <  1:7413011/151‑1 (MQ=255)
                                                                                                                          agagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTggg                         >  2:5749770/1‑151 (MQ=255)
                                                                                                                            agCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggcg                       <  1:1353079/151‑1 (MQ=255)
                                                                                                                              cAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggcggc                     <  2:10630684/151‑1 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    >  1:9005288/1‑151 (MQ=255)
                                                                                                                                  ccGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGt                 >  1:9806675/1‑151 (MQ=255)
                                                                                                                                   cGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCTCCGGTCGGAACGACCACATCCCCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAggg                >  2:1981926/1‑149 (MQ=255)
                                                                                                                                   cGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTTCAAGTCGGCCAGGCTACTGGGCGGCAAGTg                >  2:588526/1‑151 (MQ=255)
                                                                                                                                   cGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTg                <  2:1823769/151‑1 (MQ=255)
                                                                                                                                    gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGc               >  1:8512070/1‑151 (MQ=255)
                                                                                                                                    gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGc               >  1:1880749/1‑151 (MQ=255)
                                                                                                                                    gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGc               <  1:3838960/151‑1 (MQ=255)
                                                                                                                                    gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGACGGCCAGGCTACTGGGCGGCAAGTGc               <  2:2120808/151‑1 (MQ=255)
                                                                                                                                     cTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCg              >  1:9962600/1‑151 (MQ=255)
                                                                                                                                     cTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCg              >  2:3433300/1‑151 (MQ=255)
                                                                                                                                        aaCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAGGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt           >  1:6430468/1‑151 (MQ=255)
                                                                                                                                        aaCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt           <  2:5108822/151‑1 (MQ=255)
                                                                                                                                         aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGtt          >  2:7078579/1‑151 (MQ=255)
                                                                                                                                         aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGtt          >  2:16646/1‑151 (MQ=255)
                                                                                                                                           cGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCa        >  1:2724162/1‑151 (MQ=255)
                                                                                                                                            gCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCAt       >  2:2341687/1‑151 (MQ=255)
                                                                                                                                              cccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATcc     <  1:4479805/151‑1 (MQ=255)
                                                                                                                                                 ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctct  >  2:747194/1‑151 (MQ=255)
                                                                                                                                                 ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGGCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCACGTGCGAGTTCATCctct  >  1:4654770/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑