Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,407,463 A→G K125E (AAG→GAG)  PA14_RS11255 → glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,407,4630AG100.0% 1699.5 / NA 431K125E (AAG→GAG) PA14_RS11255glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (202/229);  total (202/229)

Only 100 of 433 total aligned reads displayed.

CCGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAGA  >  NC_008463/2407315‑2407610
                                                                                                                                                    |                                                                                                                                                   
ccgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                   >  2:1146144/1‑151 (MQ=255)
ccgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                   >  2:7407383/1‑151 (MQ=255)
ccgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                   <  2:5772551/151‑1 (MQ=255)
ccgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                   <  1:1974888/151‑1 (MQ=255)
  gcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGc                                                                                                                                                 >  1:5958589/1‑151 (MQ=255)
  gcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGc                                                                                                                                                 >  1:4915939/1‑151 (MQ=255)
    tATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGccc                                                                                                                                               <  2:10727391/151‑1 (MQ=255)
     aTGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCt                                                                                                                                              <  2:3416547/151‑1 (MQ=255)
       gcgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGa                                                                                                                                            >  1:2101459/1‑151 (MQ=255)
        cgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           >  1:8227273/1‑151 (MQ=255)
        cgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           >  2:4744092/1‑151 (MQ=255)
         gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                          <  1:5054814/151‑1 (MQ=255)
         gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                          >  2:8204178/1‑151 (MQ=255)
         gcCGGGCGACCTCAATCCCCACGACCCGGTGCGCCGCCCCCACGCCGACAAGTGGATGGGCAGGACCACCACGACCCTCGCCGGGCCGTTCCGCGAACTGTTATGGGGAACGCTGAGTACCCCGCCGGAGCAACGCGACGACGcccagctc                                                                                                                                          >  2:3951335/1‑146 (MQ=255)
           cGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGc                                                                                                                                        <  2:8648035/151‑1 (MQ=255)
             ggCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaa                                                                                                                                      >  2:967284/1‑151 (MQ=255)
                gACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAgg                                                                                                                                   >  1:8446612/1‑151 (MQ=255)
                gACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAgg                                                                                                                                   <  2:8830844/151‑1 (MQ=255)
                gACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAgg                                                                                                                                   >  1:2961746/1‑151 (MQ=255)
                 aCCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgc                                                                                                                                  <  1:4037467/151‑1 (MQ=255)
                 aCCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgc                                                                                                                                  >  2:8309745/1‑151 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGGTGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 <  1:5740326/151‑1 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 >  1:6814811/1‑151 (MQ=255)
                    tctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCt                                                                                                                               >  2:1020081/1‑151 (MQ=255)
                       aTCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCa                                                                                                                            >  1:7471539/1‑151 (MQ=255)
                        tCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAg                                                                                                                           <  1:4472062/151‑1 (MQ=255)
                         ccGCTGGACCCGGTGCGCCGCGCCGACGCCGCCATGTGGATGGACTGGACCAGCTCGACTCGCGCCGGGCCGTTCCGCGTCCTGTTCTGGGGAAGGATGCGTACGGCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAgg                                                                                                                          >  2:8693220/1‑150 (MQ=255)
                          cGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGAc                                                                                                                         >  2:1315278/1‑151 (MQ=255)
                              ggACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgc                                                                                                                     <  1:6700464/151‑1 (MQ=255)
                                 cccGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcggc                                                                                                                  >  1:101114/1‑151 (MQ=255)
                                       gcgccgcgccGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtg                                                                                                            <  2:6212182/151‑1 (MQ=255)
                                        cgccgcgccgACGCCGACACGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgc                                                                                                           <  2:9521924/151‑1 (MQ=255)
                                          ccgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgctg                                                                                                         <  2:707838/151‑1 (MQ=255)
                                            gcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCg                                                                                                       <  2:3691805/151‑1 (MQ=255)
                                            gcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCg                                                                                                       <  2:4474244/151‑1 (MQ=255)
                                                cgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTg                                                                                                   <  1:10185793/151‑1 (MQ=255)
                                                  acgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCACAGGCGCTGCAGACCTGCGGCGAGTAGCTGCGGGTGcc                                                                                                 >  1:2623819/1‑151 (MQ=255)
                                                       gacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAcc                                                                                            >  1:1253937/1‑151 (MQ=255)
                                                         cAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCGGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAg                                                                                          >  1:5361133/1‑151 (MQ=255)
                                                          aaGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGa                                                                                         >  1:2322201/1‑151 (MQ=255)
                                                          aaGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGa                                                                                         >  1:3577989/1‑151 (MQ=255)
                                                           aGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAc                                                                                        >  1:5242763/1‑151 (MQ=255)
                                                            gTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAcc                                                                                       <  1:7814875/151‑1 (MQ=255)
                                                            gTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAcc                                                                                       >  2:9256022/1‑151 (MQ=255)
                                                                atggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTgg                                                                                   >  2:416842/1‑151 (MQ=255)
                                                                atggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTgg                                                                                   >  2:3282872/1‑151 (MQ=255)
                                                                  ggactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggcc                                                                                 <  2:5188407/151‑1 (MQ=255)
                                                                    actggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccgg                                                                               >  1:857036/1‑151 (MQ=255)
                                                                     ctggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccggc                                                                              >  2:2698678/1‑151 (MQ=255)
                                                                      tggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccggcc                                                                             <  1:4244549/151‑1 (MQ=255)
                                                                           caccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAccc                                                                        >  2:8552914/1‑151 (MQ=255)
                                                                           caccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAccc                                                                        <  2:2088192/151‑1 (MQ=255)
                                                                            accTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                       >  1:6117189/1‑151 (MQ=255)
                                                                            accTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                       <  1:1530328/151‑1 (MQ=255)
                                                                            accTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                       <  2:6607064/151‑1 (MQ=255)
                                                                                cGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCt                                                                   <  2:10301846/151‑1 (MQ=255)
                                                                                  aCTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGt                                                                 >  1:391563/1‑151 (MQ=255)
                                                                                   ctctCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTc                                                                >  1:4774150/1‑151 (MQ=255)
                                                                                    tctcGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCATAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTcc                                                               <  2:2853208/151‑1 (MQ=255)
                                                                                      tcGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCgg                                                             <  1:4137168/151‑1 (MQ=255)
                                                                                       cGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGCCGAGGCCCTGATCGCAACGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                            <  1:622972/151‑1 (MQ=255)
                                                                                       cGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                            <  2:7693629/151‑1 (MQ=255)
                                                                                         ccGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCACAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGa                                                          <  2:5419848/151‑1 (MQ=255)
                                                                                           gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAAc                                                        <  2:4316390/151‑1 (MQ=255)
                                                                                             gCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGc                                                      >  1:9004571/1‑151 (MQ=255)
                                                                                               cGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCtt                                                    >  1:3430157/1‑151 (MQ=255)
                                                                                               cGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCtt                                                    >  2:5301519/1‑151 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   >  2:1540127/1‑151 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   <  2:9072516/151‑1 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   <  2:6420862/151‑1 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   <  1:8689768/151‑1 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   <  1:10013436/151‑1 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCGGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   <  1:1090708/151‑1 (MQ=255)
                                                                                                 ttCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCAGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCg                                                  <  1:9705640/151‑1 (MQ=255)
                                                                                                   ccGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGc                                                <  1:7088324/151‑1 (MQ=255)
                                                                                                    cgcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCa                                               <  2:10517628/151‑1 (MQ=255)
                                                                                                      cgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCAGGCTGTCCGGCGAACGCTTCGGCATg                                             >  1:7132822/1‑151 (MQ=255)
                                                                                                         ccTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGt                                          <  2:9786323/151‑1 (MQ=255)
                                                                                                           tGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGa                                        <  1:3852080/151‑1 (MQ=255)
                                                                                                            gTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGAc                                       <  2:7881964/151‑1 (MQ=255)
                                                                                                               cTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACAtt                                    <  2:7778636/151‑1 (MQ=255)
                                                                                                               cTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACAtt                                    >  1:5598996/1‑151 (MQ=255)
                                                                                                                   ggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTcccc                                <  1:6992010/151‑1 (MQ=255)
                                                                                                                    gAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTccccg                               <  2:8064887/151‑2 (MQ=255)
                                                                                                                    gAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCt                               >  1:9311579/1‑151 (MQ=255)
                                                                                                                       cgcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTggg                            <  2:8972242/149‑1 (MQ=255)
                                                                                                                           gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGTCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                        <  1:8827343/151‑1 (MQ=255)
                                                                                                                           gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                        <  2:7231336/151‑1 (MQ=255)
                                                                                                                           gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                        <  2:5013045/151‑1 (MQ=255)
                                                                                                                            cGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGtt                       <  2:6052303/151‑1 (MQ=255)
                                                                                                                               aCGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCa                    <  2:4900463/151‑1 (MQ=255)
                                                                                                                                 gccgccGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATc                  >  2:9861465/1‑151 (MQ=255)
                                                                                                                                  ccgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                 <  1:80358/151‑1 (MQ=255)
                                                                                                                                  ccgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                 <  1:9874893/151‑1 (MQ=255)
                                                                                                                                  ccgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                 <  1:3247720/151‑1 (MQ=255)
                                                                                                                                    gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAc               >  2:2430341/1‑151 (MQ=255)
                                                                                                                                       ggAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGcc            <  1:10523146/151‑1 (MQ=255)
                                                                                                                                       ggAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGcc            <  2:8764931/151‑1 (MQ=255)
                                                                                                                                             aCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTc      <  2:6776600/151‑1 (MQ=255)
                                                                                                                                                cgacgaGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCgag   <  1:10321566/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAGA  >  NC_008463/2407315‑2407610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑