Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,651,357 A→G N74D (AAC→GAC)  PA14_RS12480 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,651,3570AG100.0% 2026.6 / NA 505N74D (AAC→GAC) PA14_RS12480MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (241/264);  total (241/264)

Only 100 of 505 total aligned reads displayed.

GCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTACG  >  NC_008463/2651209‑2651504
                                                                                                                                                    |                                                                                                                                                   
gCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGAc                                                                                                                                                   <  2:1365132/151‑1 (MQ=255)
gCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGAc                                                                                                                                                   >  2:4427592/1‑151 (MQ=255)
       ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTggg                                                                                                                                            >  1:2849879/1‑151 (MQ=255)
       ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGGTggg                                                                                                                                            >  2:10061424/1‑151 (MQ=255)
        gCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgggg                                                                                                                                           >  1:5466435/1‑151 (MQ=255)
         cATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgc                                                                                                                                          >  2:3930249/1‑151 (MQ=255)
          aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcg                                                                                                                                         >  2:4805977/1‑151 (MQ=255)
            ccTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcgcg                                                                                                                                       <  2:6295551/151‑1 (MQ=255)
             ctgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcgcgc                                                                                                                                      >  2:3102176/1‑151 (MQ=255)
              tgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGcgcgcg                                                                                                                                     >  1:1855622/1‑151 (MQ=255)
              tgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGcgcgcg                                                                                                                                     >  1:1387471/1‑151 (MQ=255)
              tgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGcgcgcg                                                                                                                                     <  1:4759008/151‑1 (MQ=255)
              tgctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGcgcgcg                                                                                                                                     <  1:6711021/151‑1 (MQ=255)
               gctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcgg                                                                                                                                    >  1:8660325/1‑151 (MQ=255)
               gctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcgg                                                                                                                                    >  1:7539196/1‑151 (MQ=255)
                ctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggc                                                                                                                                   >  2:10213035/1‑151 (MQ=255)
                 tgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggcg                                                                                                                                  >  2:64545/1‑151 (MQ=255)
                    acacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGAc                                                                                                                               <  2:1053088/151‑1 (MQ=255)
                     cacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACa                                                                                                                              <  1:1043708/151‑1 (MQ=255)
                       ccTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                                            >  2:7715049/1‑151 (MQ=255)
                          tCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCt                                                                                                                         >  1:6534127/1‑151 (MQ=255)
                            aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                                       >  1:10177912/1‑151 (MQ=255)
                            aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                                       <  2:10720324/151‑1 (MQ=255)
                            aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                                       <  1:1025448/151‑1 (MQ=255)
                             gTCTGTTCATCGCACCGATGCGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgc                                                                                                                      <  1:369428/151‑2 (MQ=255)
                             gTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                                      <  1:2383043/151‑1 (MQ=255)
                               cTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGc                                                                                                                    >  1:3007101/1‑151 (MQ=255)
                                tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCt                                                                                                                   >  1:7800211/1‑151 (MQ=255)
                                    cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                                               <  2:5475217/151‑1 (MQ=255)
                                     aTCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACcg                                                                                                              <  2:4274388/151‑1 (MQ=255)
                                     aTCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACcg                                                                                                              <  1:1068755/151‑1 (MQ=255)
                                       cGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACcgcg                                                                                                            >  1:978974/1‑151 (MQ=255)
                                       cGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACcgcg                                                                                                            >  2:9156641/1‑151 (MQ=255)
                                         cACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGc                                                                                                          >  1:2205427/1‑151 (MQ=255)
                                          aCCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGcc                                                                                                         <  1:1644886/151‑1 (MQ=255)
                                           ccGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGccc                                                                                                        <  1:3486064/151‑1 (MQ=255)
                                              aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACTCTGTTCGGACTGGCGGTCGCCATCACCATCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGt                                                                                                     >  1:875709/1‑151 (MQ=255)
                                              aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGt                                                                                                     >  1:10046433/1‑151 (MQ=255)
                                                gAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcg                                                                                                   <  1:7676530/151‑1 (MQ=255)
                                                  gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgct                                                                                                 <  1:6139467/151‑1 (MQ=255)
                                                  gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgct                                                                                                 <  1:7851969/151‑1 (MQ=255)
                                                   cgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctg                                                                                                >  1:7008603/1‑151 (MQ=255)
                                                   cgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctg                                                                                                <  2:8219444/151‑1 (MQ=255)
                                                    gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                                               <  2:3788864/151‑1 (MQ=255)
                                                    gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                                               >  2:2593115/1‑151 (MQ=255)
                                                     ccGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgcc                                                                                              <  2:5074364/151‑1 (MQ=255)
                                                      cGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGGTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccg                                                                                             >  1:1233988/1‑151 (MQ=255)
                                                           aCGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                                        <  1:8008258/151‑1 (MQ=255)
                                                                tGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGcc                                                                                   >  1:7351381/1‑151 (MQ=255)
                                                                  ggACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTg                                                                                 <  2:2777941/151‑1 (MQ=255)
                                                                        cgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGgcggcg                                                                           >  2:5931403/1‑151 (MQ=255)
                                                                          ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                                         <  1:8603585/151‑1 (MQ=255)
                                                                          ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                                         <  2:6044861/151‑1 (MQ=255)
                                                                               gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATg                                                                    <  1:6406570/151‑1 (MQ=255)
                                                                               gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATg                                                                    >  2:10478073/1‑151 (MQ=255)
                                                                                   cggtGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGt                                                                >  1:5858986/4‑151 (MQ=255)
                                                                                   cGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGt                                                                <  1:8244427/151‑1 (MQ=255)
                                                                                   cGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAAGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGt                                                                <  1:4188963/151‑1 (MQ=255)
                                                                                    ggtGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTc                                                               >  1:8873345/3‑151 (MQ=255)
                                                                                      tGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTg                                                             <  1:4920593/151‑1 (MQ=255)
                                                                                       gCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTgg                                                            <  2:4663400/151‑1 (MQ=255)
                                                                                        cTGGTGACGCTGTTCGGACAGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGATGGGGCGCGCGGCGGACAATGCACGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGc                                                           >  2:5220729/1‑151 (MQ=255)
                                                                                           gTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAc                                                        <  2:8843857/151‑1 (MQ=255)
                                                                                            tGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCTAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAcg                                                       >  1:3838460/1‑150 (MQ=255)
                                                                                            tGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAct                                                       >  2:8538874/1‑151 (MQ=255)
                                                                                             gACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAActc                                                      >  1:3227576/1‑151 (MQ=255)
                                                                                              aCGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAActct                                                     <  1:1699403/151‑1 (MQ=255)
                                                                                               cGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTa                                                    >  1:4303385/1‑151 (MQ=255)
                                                                                                gCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCTGAGAATGCCGTCTGGCAACTCTAc                                                   >  1:5561745/1‑151 (MQ=255)
                                                                                                gCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAc                                                   <  2:9589652/151‑1 (MQ=255)
                                                                                                 cTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcg                                                  <  2:3705189/151‑1 (MQ=255)
                                                                                                  tGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                                                 <  1:6006596/151‑1 (MQ=255)
                                                                                                   gTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgcg                                                <  2:7180284/151‑1 (MQ=255)
                                                                                                          cTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTcc                                         <  2:252828/151‑1 (MQ=255)
                                                                                                              cGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                                     >  2:1837214/1‑151 (MQ=255)
                                                                                                               ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgctg                                    >  1:208653/1‑151 (MQ=255)
                                                                                                                gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa                                   >  2:3473571/1‑151 (MQ=255)
                                                                                                                gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa                                   <  2:2165106/151‑1 (MQ=255)
                                                                                                                gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa                                   >  1:1553689/1‑151 (MQ=255)
                                                                                                                gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa                                   >  2:6848417/1‑151 (MQ=255)
                                                                                                                gTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTGGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGa                                   >  2:5678335/1‑151 (MQ=255)
                                                                                                                  cGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCGACTCTACGCGCTGTTCCTGCTGATc                                 >  2:8665888/1‑151 (MQ=255)
                                                                                                                    ccctCACCAGCCCGCTCAAGGGCTGGCTGCTCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCgg                               <  1:2469679/148‑1 (MQ=255)
                                                                                                                    ccATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACACTGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCgg                               <  2:8038836/151‑1 (MQ=255)
                                                                                                                    ccATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCgg                               <  2:9432056/151‑1 (MQ=255)
                                                                                                                       tCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCct                            <  2:2093625/151‑1 (MQ=255)
                                                                                                                          ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct                         <  1:9129935/151‑1 (MQ=255)
                                                                                                                           cAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg                        <  1:4007906/151‑1 (MQ=255)
                                                                                                                           cAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg                        <  2:4158197/151‑1 (MQ=255)
                                                                                                                            aGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTgg                       <  1:2708737/151‑1 (MQ=255)
                                                                                                                             gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGgc                      <  1:4589728/151‑1 (MQ=255)
                                                                                                                                cgctCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGcc                   <  1:2564981/148‑1 (MQ=255)
                                                                                                                                cgctCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGcc                   <  1:4428177/148‑1 (MQ=255)
                                                                                                                                 gctCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCa                  >  2:9839536/3‑151 (MQ=255)
                                                                                                                                    cAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGc               >  2:9005782/1‑151 (MQ=255)
                                                                                                                                         gctggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCt          >  2:7412867/1‑151 (MQ=255)
                                                                                                                                           tggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGc        >  1:9583913/1‑151 (MQ=255)
                                                                                                                                               tgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTa    <  2:1651909/151‑1 (MQ=255)
                                                                                                                                               tgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTa    <  1:8169010/151‑1 (MQ=255)
                                                                                                                                                gATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTAc   >  1:5101087/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTACG  >  NC_008463/2651209‑2651504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑