Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,730,678 C→T intergenic (+16/+99) PA14_RS12860 → / ← ctpH anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,730,6780CT100.0% 1740.4 / NA 417intergenic (+16/+99)PA14_RS12860/ctpHanti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (184/233);  total (184/233)

Only 100 of 423 total aligned reads displayed.

ACTCGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGT  >  NC_008463/2730530‑2730823
                                                                                                                                                    |                                                                                                                                                 
aCTCGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAg                                                                                                                                                 >  1:6061688/1‑151 (MQ=255)
 cTCGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAgc                                                                                                                                                >  1:8119059/1‑150 (MQ=255)
 cTCGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAgc                                                                                                                                                <  1:6179059/151‑2 (MQ=255)
   cGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAgcgc                                                                                                                                              >  1:8570832/1‑148 (MQ=255)
   cGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAgcgc                                                                                                                                              >  1:3022446/1‑148 (MQ=255)
     aCGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAgcgcca                                                                                                                                            <  2:5619488/151‑6 (MQ=255)
        gccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAg                                                                                                                                         >  2:2483139/1‑151 (MQ=255)
         ccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                        <  2:8111398/151‑1 (MQ=255)
          cgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgc                                                                                                                                       >  1:7726713/1‑151 (MQ=255)
              agCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                   <  1:4408979/150‑1 (MQ=255)
               gCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTAt                                                                                                                                  <  1:9466649/151‑1 (MQ=255)
               gCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTAt                                                                                                                                  <  2:8900475/151‑1 (MQ=255)
                 tAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATcc                                                                                                                                >  1:2453836/1‑151 (MQ=255)
                    gCCGGCTGCCGCTCCACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGTCTGATCTACGAAGTGCTGGACTAAGCTCTAGACTGAGCGGGCCAGGGCGGGTAGCGCAATAGGCGCTATCCGcc                                                                                                                             <  2:3767717/151‑1 (MQ=255)
                     ccGGCGGCCGCACTACGCCGGCTACGAGTGGATGCAGGCCGGCACCGCCCGGCTGCTGGTGGCGGTGGCGACCGGGCTGATCCACGCAGTGCGGGACAACGCTCTCGACTGAGCGCGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCa                                                                                                                            <  2:9666536/151‑1 (MQ=255)
                      cGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAg                                                                                                                           >  1:1845531/1‑151 (MQ=255)
                       ggCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAgg                                                                                                                          <  1:2119955/151‑1 (MQ=255)
                          tGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGCGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGccc                                                                                                                       <  1:7963800/151‑1 (MQ=255)
                          tGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGccc                                                                                                                       >  1:3147402/1‑151 (MQ=255)
                              gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                   <  2:6053029/151‑1 (MQ=255)
                              gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                   <  1:6935007/151‑1 (MQ=255)
                                 cTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGa                                                                                                                <  1:8352704/151‑1 (MQ=255)
                                 cTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCGGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGa                                                                                                                <  2:318710/151‑1 (MQ=255)
                                  tACGCCGGCTACGCGTGGATGCAGGCCGGCACCGACCTGCTGCTCGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTCAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGat                                                                                                               <  2:784859/151‑2 (MQ=255)
                                  tACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAg                                                                                                               <  1:5622113/151‑1 (MQ=255)
                                    cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCAGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCTGGCCCCGTTGGAGTg                                                                                                             <  2:7171450/151‑1 (MQ=255)
                                     gacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                            >  2:5104299/1‑151 (MQ=255)
                                     gacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                            <  2:1751359/151‑1 (MQ=255)
                                     gacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                            >  1:9997862/1‑151 (MQ=255)
                                      acgGCTACGCGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                           <  1:7173519/151‑1 (MQ=255)
                                      acgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                           <  2:3580173/151‑1 (MQ=255)
                                      acgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                           >  2:1074860/1‑151 (MQ=255)
                                          cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAt                                                                                                       <  1:10201061/151‑1 (MQ=255)
                                          cTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAt                                                                                                       >  2:6432774/1‑151 (MQ=255)
                                            aCGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATaa                                                                                                     >  1:1308036/1‑151 (MQ=255)
                                               aGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCg                                                                                                  >  2:10261895/1‑151 (MQ=255)
                                                  ggATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtgt                                                                                               >  1:4990617/1‑151 (MQ=255)
                                                     tGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgc                                                                                            <  1:5177264/151‑1 (MQ=255)
                                                       cAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcgc                                                                                          >  2:1897512/1‑151 (MQ=255)
                                                            cGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTc                                                                                     <  1:1086610/151‑1 (MQ=255)
                                                            cGGCACCGACCTGCTGCTGGTGGCGGGGGCGACCGGGCTGATCTACGAAGTGCTGGACACCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTgcc                                                                                     <  1:6615415/151‑3 (MQ=255)
                                                              gCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCg                                                                                   >  2:4382017/1‑151 (MQ=255)
                                                                 ccGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGccc                                                                                >  1:4667993/1‑151 (MQ=255)
                                                                  cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                               >  1:5929979/1‑151 (MQ=255)
                                                                  cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                               <  1:10313665/151‑1 (MQ=255)
                                                                   gACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTa                                                                              <  1:9016394/151‑1 (MQ=255)
                                                                      ctgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCt                                                                           <  1:5839279/151‑1 (MQ=255)
                                                                      ctgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCt                                                                           >  2:7764892/1‑151 (MQ=255)
                                                                       tgctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTc                                                                          <  2:5908437/151‑1 (MQ=255)
                                                                        gctgctgGTGGCGGTGGCGACCGGGCTGATCGACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCCTGTTGCGCTTGTCCGCCCTACCTCg                                                                         <  2:663604/151‑1 (MQ=255)
                                                                         ctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGt                                                                        >  2:1482875/1‑151 (MQ=255)
                                                                          tgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTg                                                                       >  1:36250/1‑151 (MQ=255)
                                                                           gctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTgg                                                                      >  1:9115910/1‑151 (MQ=255)
                                                                            ctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGa                                                                     <  1:421733/151‑1 (MQ=255)
                                                                            ctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGa                                                                     >  1:5322924/1‑151 (MQ=255)
                                                                             tggtggCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGaa                                                                    <  1:2563863/151‑1 (MQ=255)
                                                                              ggtggcggtggcGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAg                                                                   <  1:9370678/151‑1 (MQ=255)
                                                                                tggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTc                                                                 <  2:7054754/151‑1 (MQ=255)
                                                                                    ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             <  1:7447853/151‑1 (MQ=255)
                                                                                    ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             >  1:5455500/1‑151 (MQ=255)
                                                                                    ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             <  2:3396278/151‑1 (MQ=255)
                                                                                         cgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGACGTCCTGTTCTTa                                                        >  2:10523083/1‑151 (MQ=255)
                                                                                         cgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTa                                                        <  2:4759615/151‑1 (MQ=255)
                                                                                           aCCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcc                                                      <  1:10169787/151‑1 (MQ=255)
                                                                                              gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                   <  1:4795537/151‑1 (MQ=255)
                                                                                              gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                   <  2:4020221/151‑1 (MQ=255)
                                                                                                gCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTCCCTCGTGGAAGTCCTTTTCTTACCCCCgg                                                 <  2:2817237/151‑1 (MQ=255)
                                                                                                gCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAgg                                                 >  2:9856920/1‑151 (MQ=255)
                                                                                                gCTGATATACTAAGTGCTGTACAAAGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCATGCCCCGTTGGAGTGGCGGATAACCGTTTTGCGCTTGTCAGCCCTACCTCGTGTAAGTACTTTTCTTTCCCCAgg                                                 <  2:5288593/151‑1 (MQ=255)
                                                                                                 cTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTTTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGt                                                <  2:7465295/151‑1 (MQ=255)
                                                                                                 cTGATCCACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAgct                                                <  2:9483313/151‑3 (MQ=255)
                                                                                                    aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAg                                             <  1:10660758/151‑1 (MQ=255)
                                                                                                     tCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAgg                                            >  1:1580258/1‑151 (MQ=255)
                                                                                                         cGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCg                                        <  1:10000557/151‑1 (MQ=255)
                                                                                                          gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGc                                       <  1:7286342/151‑1 (MQ=255)
                                                                                                          gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGc                                       <  1:1914828/151‑1 (MQ=255)
                                                                                                             gTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAgg                                    <  2:2643076/151‑1 (MQ=255)
                                                                                                               gCTGGACAACGCTCTCGACGGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCg                                  <  2:8398627/151‑1 (MQ=255)
                                                                                                                 tGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGtt                                >  2:2468718/1‑151 (MQ=255)
                                                                                                                  ggACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTg                               >  2:9414291/1‑151 (MQ=255)
                                                                                                                   gACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                              >  1:207804/1‑151 (MQ=255)
                                                                                                                     cAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAg                            >  1:3369797/1‑151 (MQ=255)
                                                                                                                      aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAgg                           >  1:9867049/1‑151 (MQ=255)
                                                                                                                      aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAgg                           <  1:6754640/151‑1 (MQ=255)
                                                                                                                      aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAgg                           >  2:10131713/1‑151 (MQ=255)
                                                                                                                        cGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGc                         <  2:8476831/151‑1 (MQ=255)
                                                                                                                            ctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCg                     <  2:3041786/151‑1 (MQ=255)
                                                                                                                            ctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCg                     <  1:2662440/151‑1 (MQ=255)
                                                                                                                              cGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGGAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGcc                   >  2:6847839/1‑151 (MQ=255)
                                                                                                                               gACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGccc                  <  2:9088780/151‑1 (MQ=255)
                                                                                                                                  tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               <  1:646700/151‑1 (MQ=255)
                                                                                                                                  tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               >  2:9178174/1‑151 (MQ=255)
                                                                                                                                  tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               <  2:4597209/151‑1 (MQ=255)
                                                                                                                                   gAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGtt              <  1:8950462/151‑1 (MQ=255)
                                                                                                                                    aGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTg             <  2:8716140/151‑1 (MQ=255)
                                                                                                                                          ccAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGga       >  2:6578779/1‑151 (MQ=255)
                                                                                                                                          ccAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGga       >  2:1255595/1‑151 (MQ=255)
                                                                                                                                              ggcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAgg   >  1:1386825/1‑151 (MQ=255)
                                                                                                                                               gcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGt  <  2:7238653/151‑1 (MQ=255)
                                                                                                                                               gcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGt  <  1:8773489/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTCGACGGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGT  >  NC_008463/2730530‑2730823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑