Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,150,987 Δ1 bp intergenic (‑192/+24) pvcA ← / ← PA14_RS14420 paerucumarin biosynthesis protein PvcA/asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,150,9850C.100.0% 1964.4 / NA 395intergenic (‑190/+26)pvcA/PA14_RS14420paerucumarin biosynthesis protein PvcA/asparaginase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (203/192);  total (203/192)

Only 100 of 416 total aligned reads displayed.

GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131
                                                                                                                                                      |                                                                                                                                                  
ggCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGc                                                                                                                                                    <  1:6407894/151‑1 (MQ=255)
  cAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccg                                                                                                                                                  >  2:9364542/1‑150 (MQ=255)
   aGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccgc                                                                                                                                                 <  2:6110628/151‑3 (MQ=255)
     cGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGGGTTGTCGCGTCTCGCGGGCAGCTCGGCCGGAAGCGGccgccg                                                                                                                                               >  1:8321316/1‑147 (MQ=255)
     cGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccgccg                                                                                                                                               >  2:3841677/1‑147 (MQ=255)
     cGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccgccg                                                                                                                                               <  2:10235937/151‑5 (MQ=255)
      ggAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTCTCGCGGGGAGCTCGGACGGAAGCGG‑CCGCcgg                                                                                                                                             >  2:231049/1‑151 (MQ=255)
       gAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcggc                                                                                                                                            >  2:5970418/1‑151 (MQ=255)
       gAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcggc                                                                                                                                            <  1:4329663/151‑1 (MQ=255)
       gAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcggc                                                                                                                                            >  1:9359078/1‑151 (MQ=255)
           gCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGATGGAAGCGG‑CCGCCggcgggc                                                                                                                                        >  2:4865964/1‑151 (MQ=255)
           gCGACATCCTGTGGACGCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCggcgggc                                                                                                                                        <  2:7760957/151‑1 (MQ=255)
            cGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCggcgggcg                                                                                                                                       <  1:9734058/151‑1 (MQ=255)
               cATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGcc                                                                                                                                    <  1:8646529/151‑1 (MQ=255)
                aTCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGTGGGGGCCGCCGGCGTTGACGGGTAGCGCGGGGAGGTCGGATGGAAGCGG‑CCGCCGGCTGGAGGCCg                                                                                                                                   >  1:4167278/1‑151 (MQ=255)
                      tgGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGACACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGc                                                                                                                             <  1:2648362/151‑1 (MQ=255)
                      tgGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGc                                                                                                                             >  1:4790138/1‑151 (MQ=255)
                      tgGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGc                                                                                                                             >  2:4417260/1‑151 (MQ=255)
                         ccTCGCCGCCGGGGGATAAACCGGGGATGGCCGGAAACCCTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTTTCGCTTATCGCGGGGATCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGt                                                                                                                          <  1:2957343/150‑1 (MQ=255)
                             gccgccGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  1:10249490/1‑151 (MQ=255)
                             gccgccGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  2:5515091/1‑151 (MQ=255)
                              ccgccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTc                                                                                                                     <  2:8243320/151‑1 (MQ=255)
                               cgccgGGGGGAAAAACGGGGAAGGCCGGAAACACTGTTCGCAGAATCTTGAAGAAGGGCGCCGGGCCCGGGCGGGCCCCCGGCGGTGTCGCGTATAGCGGGGAGCACCGAATGAAGCGG‑CCGCCGGCGGGCGGCCGCCGGGCGGTCAGTCg                                                                                                                    >  2:9409562/1‑151 (MQ=255)
                                 ccgGGGGATAACCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGc                                                                                                                  <  1:1417018/151‑1 (MQ=255)
                                 ccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCACGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGc                                                                                                                  >  1:872862/1‑151 (MQ=255)
                                  cgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCg                                                                                                                 >  1:1717533/1‑151 (MQ=255)
                                   gggggATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGt                                                                                                                >  1:1558102/1‑151 (MQ=255)
                                     gggATAACCCGGGGAAGGCCGGAAACACTGTTCGCTTAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTtc                                                                                                              <  1:9249203/151‑1 (MQ=255)
                                        atcaaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcg                                                                                                           <  2:4096902/148‑1 (MQ=255)
                                          aaaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgcc                                                                                                         >  2:4370970/1‑151 (MQ=255)
                                           aaCCGGGGAAGGCCGGACACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGGCGCGTTTCGCGGGGAGCGCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgccg                                                                                                        >  1:3689086/1‑151 (MQ=255)
                                             ccGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGca                                                                                                      <  2:7540542/151‑1 (MQ=255)
                                              cGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGcac                                                                                                     >  2:10449120/1‑151 (MQ=255)
                                                 ggAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAgg                                                                                                  >  2:4730660/1‑151 (MQ=255)
                                                  gAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGt                                                                                                 >  1:10019306/1‑151 (MQ=255)
                                                  gAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGt                                                                                                 <  2:1438334/151‑1 (MQ=255)
                                                  gAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGt                                                                                                 <  1:1160078/151‑1 (MQ=255)
                                                   aaGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTc                                                                                                >  2:2905885/1‑151 (MQ=255)
                                                    aGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCg                                                                                               <  1:6693000/151‑1 (MQ=255)
                                                        cGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAggg                                                                                           >  1:2549827/1‑151 (MQ=255)
                                                         ggAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          >  2:6426213/1‑151 (MQ=255)
                                                            aaCACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGaa                                                                                       >  2:789871/1‑151 (MQ=255)
                                                             acacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAc                                                                                      <  1:1780958/151‑1 (MQ=255)
                                                              cacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAcc                                                                                     >  2:5055104/1‑151 (MQ=255)
                                                              cacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAcc                                                                                     >  1:790272/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    >  1:5411046/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGATGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    >  1:3653493/1‑151 (MQ=255)
                                                                  gTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCg                                                                                 >  2:1962870/1‑151 (MQ=255)
                                                                       cTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgcg                                                                            >  2:1589732/1‑151 (MQ=255)
                                                                        tGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgcgc                                                                           >  1:6914524/1‑151 (MQ=255)
                                                                         gAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCt                                                                          <  2:3569174/151‑1 (MQ=255)
                                                                          atgCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCGGGCGTTGTCGCTTTTCGCGGGGCGCTCGGATGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCtt                                                                         <  1:6085781/149‑1 (MQ=255)
                                                                            gCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcg                                                                       <  1:6791926/151‑1 (MQ=255)
                                                                                gACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgc                                                                   <  2:10813582/151‑1 (MQ=255)
                                                                                 aCGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgcg                                                                  <  1:7692710/151‑1 (MQ=255)
                                                                                 aCGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgcg                                                                  >  1:2936314/1‑151 (MQ=255)
                                                                                  cGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACTGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGt                                                                 >  1:8861315/1‑151 (MQ=255)
                                                                                    aTGGGCGCCGGGCACGGGCGGGCCGCCTGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTcc                                                               <  2:6342928/151‑1 (MQ=255)
                                                                                       ggCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAg                                                            <  2:10110800/151‑1 (MQ=255)
                                                                                         cgcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGCGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGTACCAGCGTTGCGCTTCGTCGCGTCCGAGGc                                                          <  2:7177715/151‑1 (MQ=255)
                                                                                         cgcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGc                                                          <  1:3134356/151‑1 (MQ=255)
                                                                                         cgcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGc                                                          >  2:213625/1‑151 (MQ=255)
                                                                                           ccGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCg                                                        <  2:1480608/151‑1 (MQ=255)
                                                                                            cGGGCACGGGCGGGCCGCCGGCGTTGTCGCTTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCgg                                                       >  2:233980/1‑151 (MQ=255)
                                                                                            cGGGCACGGGCGGGCCGCCGGCGGTGTCGGGTATCTCGGGGAGCTCGGCAGGACGCGG‑CCGCCGGAGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGCGCGCGCGTTGCGCTTCGTCGCGTCGGGGGGCgg                                                       >  1:2771719/1‑151 (MQ=255)
                                                                                             gggCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgc                                                      <  1:3898764/151‑1 (MQ=255)
                                                                                             gggCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgc                                                      >  2:3152843/1‑151 (MQ=255)
                                                                                                cACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGcc                                                   <  2:4959909/151‑1 (MQ=255)
                                                                                                cACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGcc                                                   >  2:10611650/1‑151 (MQ=255)
                                                                                                   gggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAg                                                >  1:190451/1‑151 (MQ=255)
                                                                                                      cgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAg                                             >  1:9017291/1‑151 (MQ=255)
                                                                                                      cgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAg                                             <  2:8977537/151‑1 (MQ=255)
                                                                                                        ggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGc                                           <  2:3096275/151‑1 (MQ=255)
                                                                                                        ggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGc                                           >  1:7234746/1‑151 (MQ=255)
                                                                                                        ggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGc                                           <  1:6556248/151‑1 (MQ=255)
                                                                                                        ggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGc                                           >  1:10495512/1‑151 (MQ=255)
                                                                                                          ccgccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCg                                         >  1:50350/1‑151 (MQ=255)
                                                                                                          ccgccgGCGGTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCg                                         <  1:9846493/151‑1 (MQ=255)
                                                                                                            gccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGaa                                       >  1:5790449/1‑151 (MQ=255)
                                                                                                              cgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAaga                                     <  1:2295548/151‑1 (MQ=255)
                                                                                                               ggCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAagag                                    <  1:10822971/151‑1 (MQ=255)
                                                                                                                    tGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGc                               <  2:1242390/151‑1 (MQ=255)
                                                                                                                    tGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGc                               <  1:4754477/151‑1 (MQ=255)
                                                                                                                        gcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAg                           >  1:10689101/1‑151 (MQ=255)
                                                                                                                        gcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAg                           >  2:7848086/1‑151 (MQ=255)
                                                                                                                        gcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAg                           >  2:2417871/1‑151 (MQ=255)
                                                                                                                          gTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAggg                         <  2:7447731/151‑1 (MQ=255)
                                                                                                                            aTCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgcc                       <  1:5314501/151‑1 (MQ=255)
                                                                                                                               gcgGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgccgcc                    >  2:953208/1‑151 (MQ=255)
                                                                                                                               gcgGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgccgcc                    <  2:1796018/151‑1 (MQ=255)
                                                                                                                                 ggggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTc                  <  1:9535807/151‑1 (MQ=255)
                                                                                                                                 ggggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTc                  <  1:4622957/151‑1 (MQ=255)
                                                                                                                                  gggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTcg                 >  1:70377/1‑151 (MQ=255)
                                                                                                                                  gggAGCGCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCCGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGTGCAGGCCGAAGTGCTTGCCCAGGGCCGCCTcg                 >  1:447196/1‑151 (MQ=255)
                                                                                                                                   ggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTcgc                >  2:2686321/1‑151 (MQ=255)
                                                                                                                                   ggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTcgc                >  2:8714250/1‑151 (MQ=255)
                                                                                                                                     aGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCgg              <  1:3953729/151‑1 (MQ=255)
                                                                                                                                      gCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCggg             <  2:10360066/151‑1 (MQ=255)
                                                                                                                                         cGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCa          >  1:3345010/1‑151 (MQ=255)
                                                                                                                                                 agCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTccacca  <  2:5919740/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑