BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,722,861	Δ2 bp	coding (400‑401/1527 nt)	PA14_RS16920 →	inactive transglutaminase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,722,861	0	G	.	100.0%	1834.7 / NA	368	coding (400/1527 nt)	PA14_RS16920	inactive transglutaminase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (159/209); total (159/209)
*	NC_008463	3,722,862	0	A	.	100.0%	1834.7 / NA	368	coding (401/1527 nt)	PA14_RS16920	inactive transglutaminase family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (159/209); total (159/209)

Only 100 of 368 total aligned reads displayed.

TCAGCATCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGCGACAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTCAAGCTG  >  NC_008463/3722718‑3723004

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  aGCATCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGccc                                                                                                                                      >  1:4888539/1‑151 (MQ=255)

  aGCATCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGccc                                                                                                                                      <  2:6671737/151‑1 (MQ=255)

   gCATCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGcccc                                                                                                                                     >  1:9653290/1‑151 (MQ=255)

      tCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTgg                                                                                                                                  >  2:8800771/1‑151 (MQ=255)

      tCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTgg                                                                                                                                  <  1:5327794/151‑1 (MQ=255)

       cAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCACGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGa                                                                                                                                 >  1:3128780/1‑151 (MQ=255)

       cAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGa                                                                                                                                 <  2:10742123/151‑1 (MQ=255)

         aTCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAg                                                                                                                               <  1:8876732/151‑1 (MQ=255)

           cgcgCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGc                                                                                                                             <  1:10817796/151‑1 (MQ=255)

             cgcCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGccc                                                                                                                           <  1:2795641/151‑1 (MQ=255)

              gcCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCg                                                                                                                          <  1:2480922/151‑1 (MQ=255)

                cGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGga                                                                                                                        >  1:2356214/1‑151 (MQ=255)

                 gACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGgag                                                                                                                       >  1:2205832/1‑151 (MQ=255)

                 gACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGgag                                                                                                                       <  2:7400445/151‑1 (MQ=255)

                     gCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGTAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGagaaga                                                                                                                   <  2:1669218/151‑1 (MQ=255)

                     gCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGagaaga                                                                                                                   <  2:130895/151‑1 (MQ=255)

                     gCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGagaaga                                                                                                                   >  2:8307362/1‑151 (MQ=255)

                      cAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGAt                                                                                                                  <  1:5472918/151‑1 (MQ=255)

                      cAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGAt                                                                                                                  <  1:6670062/151‑1 (MQ=255)

                        aTCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATcg                                                                                                                <  2:2748124/151‑1 (MQ=255)

                         tCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATcgc                                                                                                               <  1:1851424/151‑1 (MQ=255)

                           gCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATcgccg                                                                                                             <  1:2111474/151‑1 (MQ=255)

                              aGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAg                                                                                                          <  2:7845983/151‑1 (MQ=255)

                               ggTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGa                                                                                                         <  2:7223609/151‑1 (MQ=255)

                                gTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGaa                                                                                                        <  1:9518729/151‑1 (MQ=255)

                                  gACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGc                                                                                                      >  2:1571294/1‑151 (MQ=255)

                                   aCCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGcc                                                                                                     >  1:5836807/1‑151 (MQ=255)

                                    ccTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGccc                                                                                                    >  2:6156006/1‑151 (MQ=255)

                                     cTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCct                                                                                                   <  2:6894070/151‑1 (MQ=255)

                                        gTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCctgct                                                                                                <  2:2574670/151‑1 (MQ=255)

                                         tCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCctgctg                                                                                               <  2:4952002/151‑1 (MQ=255)

                                          ccgcccgcCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTgg                                                                                              >  1:9922765/1‑151 (MQ=255)

                                             cccgccGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGTCCAAGGAGAAAGGTCCGATCTTCAGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGccc                                                                                           >  2:5305463/1‑151 (MQ=255)

                                                  cgcgCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCAtt                                                                                      <  2:2960570/151‑1 (MQ=255)

                                                    cgcCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCg                                                                                    <  1:9532580/151‑1 (MQ=255)

                                                    cgcCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCg                                                                                    >  1:7690843/1‑151 (MQ=255)

                                                    cgcCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCg                                                                                    >  1:1958301/1‑151 (MQ=255)

                                                      ccAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGcc                                                                                  >  2:10470474/1‑151 (MQ=255)

                                                      ccAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGcc                                                                                  >  2:9757972/1‑151 (MQ=255)

                                                       cAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCa                                                                                 >  2:8549127/1‑151 (MQ=255)

                                                       cAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCAGTCGCCa                                                                                 >  1:7458205/1‑151 (MQ=255)

                                                         aCGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGc                                                                               >  1:4401136/1‑151 (MQ=255)

                                                          cGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCa                                                                              <  2:2109165/151‑1 (MQ=255)

                                                            gCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCCAGGAGAAACGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCAcg                                                                            >  2:7137957/1‑150 (MQ=255)

                                                             cAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTc                                                                           >  2:8460578/1‑151 (MQ=255)

                                                               aGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCgg                                                                         <  1:1586238/151‑1 (MQ=255)

                                                                  aGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCg                                                                      <  2:4573573/151‑1 (MQ=255)

                                                                  aGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCAGCCg                                                                      <  1:10265462/151‑1 (MQ=255)

                                                                      ccTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGt                                                                  <  2:558798/151‑1 (MQ=255)

                                                                       cTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCTAGAAGCCCATGTAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTc                                                                 <  2:32867/151‑1 (MQ=255)

                                                                       cTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTc                                                                 <  2:6037596/151‑1 (MQ=255)

                                                                        tGTACTACCGCCTGGTGCTGACCAAGCGCGACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCg                                                                <  2:9100812/151‑1 (MQ=255)

                                                                         gTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCga                                                               <  2:7448967/151‑1 (MQ=255)

                                                                           actacCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCgaga                                                             >  1:6135765/1‑151 (MQ=255)

                                                                             tacCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGAcc                                                           <  1:10560024/151‑1 (MQ=255)

                                                                                 gCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCg                                                       <  2:3719749/151‑1 (MQ=255)

                                                                                    tGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCa                                                    >  2:9348818/1‑151 (MQ=255)

                                                                                     ggTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAg                                                   <  1:5157430/151‑1 (MQ=255)

                                                                                       tGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCg                                                 >  1:3833479/1‑151 (MQ=255)

                                                                                        gCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGa                                                <  2:3505557/151‑1 (MQ=255)

                                                                                         cTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGaa                                               <  1:1258318/151‑1 (MQ=255)

                                                                                          tGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAg                                              <  1:3667814/151‑1 (MQ=255)

                                                                                            aCCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGcc                                            <  1:9447359/151‑1 (MQ=255)

                                                                                             ccAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCa                                           >  1:7961008/1‑151 (MQ=255)

                                                                                              cAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCAt                                          <  2:10675157/151‑1 (MQ=255)

                                                                                                 gcgcTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCaa                                       >  1:5927655/1‑151 (MQ=255)

                                                                                                  cgcTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAg                                      <  1:4094912/151‑1 (MQ=255)

                                                                                                    cTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcg                                    >  1:6440507/1‑151 (MQ=255)

                                                                                                      acacCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcgcg                                  >  2:2294135/1‑151 (MQ=255)

                                                                                                      acacCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcgcg                                  >  1:154135/1‑151 (MQ=255)

                                                                                                      acacCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcgcg                                  >  1:10375094/1‑151 (MQ=255)

                                                                                                      acacCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcgcg                                  <  1:8555377/151‑1 (MQ=255)

                                                                                                       cacCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAgcgcgc                                 <  1:4613238/151‑1 (MQ=255)

                                                                                                         cctgCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCca                               <  2:8052325/148‑1 (MQ=255)

                                                                                                             cGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCcaacaa                           <  2:4338271/151‑1 (MQ=255)

                                                                                                              gagaAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCaacaac                          >  2:10224648/1‑151 (MQ=255)

                                                                                                              gagaAGACCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCaacaac                          <  2:4167888/151‑1 (MQ=255)

                                                                                                               agaagaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACg                         >  1:3536451/1‑151 (MQ=255)

                                                                                                               agaagaCCAAGGAGAAAGGTCCGATCGTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACg                         <  2:1918352/151‑1 (MQ=255)

                                                                                                                gaagaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTGGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACgg                        <  1:4515369/151‑1 (MQ=255)

                                                                                                                gaagaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACgg                        <  1:7261087/151‑1 (MQ=255)

                                                                                                                gaagaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACgg                        <  1:7493657/151‑1 (MQ=255)

                                                                                                                   gaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCaa                     <  2:6454887/151‑1 (MQ=255)

                                                                                                                   gaCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCaa                     <  2:9591912/151‑1 (MQ=255)

                                                                                                                    aCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCTCTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAac                    <  1:9074474/151‑1 (MQ=255)

                                                                                                                    aCCAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAac                    >  1:5602352/1‑151 (MQ=255)

                                                                                                                     ccAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAacg                   >  2:4487746/1‑151 (MQ=255)

                                                                                                                     ccAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAacg                   <  2:7952809/151‑1 (MQ=255)

                                                                                                                      cAAGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAacga                  >  2:3686581/1‑151 (MQ=255)

                                                                                                                       taGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGATGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCTGCACTCGGCCGACGTCGAGACCTTCGTCAGCGTAGCCATCAAGCGCGCCTACAACGGCAacgac                 <  2:1384736/150‑1 (MQ=255)

                                                                                                                       aaGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAacgac                 <  1:4783420/151‑1 (MQ=255)

                                                                                                                        aGGAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAacgacg                <  2:6298557/151‑1 (MQ=255)

                                                                                                                         ggAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAAcgacga               >  1:9264102/1‑151 (MQ=255)

                                                                                                                         ggAGAAAGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAAcgacga               >  1:7853064/1‑151 (MQ=255)

                                                                                                                             aaaGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAAc           >  2:4582121/1‑151 (MQ=255)

                                                                                                                               aGGTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGt         >  2:5922484/1‑151 (MQ=255)

                                                                                                                                ggTCCGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTc        <  2:9151446/151‑1 (MQ=255)

                                                                                                                                   ccGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTCAAg     >  2:8249387/1‑151 (MQ=255)

                                                                                                                                    cGATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTCAAgc    >  1:1020290/1‑151 (MQ=255)

                                                                                                                                     gATCTTCCGC‑‑CAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTCAAgct   >  1:8405018/1‑151 (MQ=255)

||

TCAGCATCAATCGCGCCGACGGCAATCGCAAGGTGACCTGGTCCGCCCGCCGCGCCAACGGCAAGCAGACCCTGTACTACCGCCTGGTGCTGACCAAGCGCTACACCGGCGAGAAGACCAAGGAGAAAGGTCCGATCTTCCGCGACAGCCTGCCCCTGGAAGGCCCGGAGAAGATCGCCGCAGAAGCCCTGCTGGCCCCCATTCGCCAGCACTCGGCCGACGTCGAGACCTTCGTCAGCGAAGCCATCAAGCGCGCCAACAACGGCAACGACGACAACGTCAAGCTG  >  NC_008463/3722718‑3723004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`