Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,514,060 (G)5→4 intergenic (+15/+136) PA14_RS25260 → / ← PA14_RS25265 tRNA‑Met/HigA family addiction module antitoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,514,0560G.99.4% 1767.4 / 2.7 361intergenic (+11/+140)PA14_RS25260/PA14_RS25265tRNA‑Met/HigA family addiction module antitoxin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (177/181);  minor base A (2/0);  total (180/181)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 390 total aligned reads displayed.

AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCC  >  NC_008463/5513906‑5514201
                                                                                                                                                      |                                                                                                                                                 
aGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAg                                                                                                                                                   >  1:3099041/1‑151 (MQ=255)
aGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAg                                                                                                                                                   <  2:3879145/151‑1 (MQ=255)
  aaaaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggg                                                                                                                                                 >  1:2404428/1‑151 (MQ=255)
    aaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggt                                                                                                                                               >  1:464722/1‑150 (MQ=255)
    aaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggt                                                                                                                                               >  1:7792199/1‑150 (MQ=255)
     aTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggtt                                                                                                                                              <  1:2449108/151‑3 (MQ=255)
      ttATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggtta                                                                                                                                             >  1:6102722/1‑148 (MQ=255)
       tATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggttag                                                                                                                                            <  1:9597005/151‑5 (MQ=255)
       tATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggttag                                                                                                                                            >  1:8487609/1‑147 (MQ=255)
       tATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggttag                                                                                                                                            <  2:7880117/151‑5 (MQ=255)
        aTCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgc                                                                                                                                          <  1:9190545/151‑1 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                       >  2:3769579/1‑151 (MQ=255)
           tAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCa                                                                                                                                       <  1:2912854/151‑1 (MQ=255)
            aGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCaa                                                                                                                                      >  2:7619336/1‑151 (MQ=255)
             ggCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAg                                                                                                                                     <  1:9924543/151‑1 (MQ=255)
             ggCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACAGAGCTCAGTCGGTTAGAGCGCAGCATTCATAAGGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTT‑AAAGGGGTTAGCGCAAg                                                                                                                                     >  2:3233684/1‑151 (MQ=255)
              gCCCAGGGGTTGACAGCCCCGCCCGATATACGGAAAAAGCGAGCCACTCGGCTACATACCTCAGTCGGTGAGATCGCATCATACATAATGCTGATGTCCCAGGTTCAAGTCACGGTGTAGCAACCATATTTTTCAA‑GGGGTTAGCGCAAGc                                                                                                                                    >  2:5073607/1‑151 (MQ=255)
               cccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCt                                                                                                                                   >  2:4951026/1‑151 (MQ=255)
                   ggggTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAAcc                                                                                                                               >  1:10116629/1‑151 (MQ=255)
                      gTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCt                                                                                                                            <  1:9363183/151‑1 (MQ=255)
                      gTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCt                                                                                                                            >  1:7402582/1‑151 (MQ=255)
                       ttGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCtt                                                                                                                           <  2:9879970/151‑1 (MQ=255)
                       ttGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTGTTCAA‑GGGGTTAGCGCAAGCTAACCCCtt                                                                                                                           >  1:4268699/1‑151 (MQ=255)
                         gACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCtttt                                                                                                                         <  2:4545266/151‑1 (MQ=255)
                          aCAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttttt                                                                                                                        <  1:2611600/151‑1 (MQ=255)
                          aCAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTC‑ATGGGGTTAGCGCAAGCTAACCCCttttt                                                                                                                        >  2:7474382/1‑151 (MQ=255)
                              ccccGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgttt                                                                                                                    >  2:8921880/1‑151 (MQ=255)
                              ccccGCCCGAGATACGGAAAAGGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgttt                                                                                                                    <  2:4839483/151‑1 (MQ=255)
                               cccgcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtttt                                                                                                                   >  1:9756357/1‑151 (MQ=255)
                                  gcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCTA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGt                                                                                                                >  1:4139862/1‑151 (MQ=255)
                                    ccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCg                                                                                                              >  2:2992107/1‑151 (MQ=255)
                                      gagaTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGc                                                                                                            >  1:1549619/1‑151 (MQ=255)
                                       agaTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCa                                                                                                           >  2:5739819/1‑151 (MQ=255)
                                          tACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTCACCCCTTTTTGTTTTGGTCGGCAgac                                                                                                        >  2:6936177/1‑151 (MQ=255)
                                           aCGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgact                                                                                                       >  1:5228812/1‑151 (MQ=255)
                                             ggAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactac                                                                                                     <  2:5956997/151‑1 (MQ=255)
                                               aaaaTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaa                                                                                                   >  2:1402557/1‑151 (MQ=255)
                                                aaaTGCGCGCCACTAGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaa                                                                                                  <  1:10024678/151‑1 (MQ=255)
                                                  aTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaacc                                                                                                <  1:2728992/151‑1 (MQ=255)
                                                    gcgcgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccga                                                                                              >  1:183954/1‑151 (MQ=255)
                                                      gcgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgact                                                                                            <  2:8713974/151‑1 (MQ=255)
                                                       cgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgacta                                                                                           >  2:2738322/1‑151 (MQ=255)
                                                        gcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactac                                                                                          >  1:7510490/1‑151 (MQ=255)
                                                        gcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactac                                                                                          <  1:545844/151‑1 (MQ=255)
                                                         ccACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactaca                                                                                         <  1:3278165/151‑1 (MQ=255)
                                                          cACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgacgacaa                                                                                        <  2:10215392/151‑1 (MQ=255)
                                                            cTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaac                                                                                      >  1:5824416/1‑151 (MQ=255)
                                                              cGGCTACATACCTCAGTCGCTTAGAGCGCAGCATACATAAGGCTGCTGGCCCAGGTTCAAGTCCCGGTGTAGCCACCAAAATTTTCAA‑GGGGTTAGCGCAAGCGAACCCCTTTTTGATTTGGACGGCAGactacaaaccgactacaaaccg                                                                                    <  2:1968111/151‑1 (MQ=255)
                                                                gCTACATAGCTCAGTCGGTTAGAGCGCAGCCTTCATAATGCTGATGGCCCAGGGTCAAGTCCCGGTGTAGCCACCATAACTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTATTTGTTGTCGTCGGCAGACTACAAACCGACTACAAACCGTc                                                                                  <  2:10054685/151‑1 (MQ=255)
                                                                 cTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCt                                                                                 <  2:9266611/151‑1 (MQ=255)
                                                                    cATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACg                                                                              <  1:1455995/151‑1 (MQ=255)
                                                                    cATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACg                                                                              <  1:4442321/151‑1 (MQ=255)
                                                                     aTAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGc                                                                             >  1:2269879/1‑151 (MQ=255)
                                                                      tAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGcc                                                                            >  1:3499498/1‑151 (MQ=255)
                                                                      tAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGcc                                                                            <  1:1353613/151‑1 (MQ=255)
                                                                          tCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCt                                                                        <  1:3013691/151‑1 (MQ=255)
                                                                          tCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTGTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCt                                                                        >  2:3353666/1‑151 (MQ=255)
                                                                            aGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCttt                                                                      >  2:95473/1‑151 (MQ=255)
                                                                             gTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTa                                                                     >  1:8068693/1‑151 (MQ=255)
                                                                               cGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTCACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACACACCGTCTACGCCCCCTTTAcc                                                                   >  2:2323423/1‑151 (MQ=255)
                                                                                ggTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCg                                                                  >  2:152274/1‑151 (MQ=255)
                                                                                ggTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCAGATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCTGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCg                                                                  <  2:8319594/151‑1 (MQ=255)
                                                                                 gTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGa                                                                 >  1:5696811/1‑151 (MQ=255)
                                                                                   tAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAtt                                                               <  1:6869329/151‑1 (MQ=255)
                                                                                   tAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTGTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAtt                                                               >  2:10488395/1‑151 (MQ=255)
                                                                                    agagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAttt                                                              >  2:601140/1‑151 (MQ=255)
                                                                                    agagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAttt                                                              >  1:6674542/1‑151 (MQ=255)
                                                                                     gagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTa                                                             >  2:2508511/1‑151 (MQ=255)
                                                                                     gagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTa                                                             <  1:8579648/151‑1 (MQ=255)
                                                                                         gcagcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGt                                                         <  1:9024593/151‑1 (MQ=255)
                                                                                          cagcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGtt                                                        <  1:492556/151‑1 (MQ=255)
                                                                                            gcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCg                                                      <  1:3819654/151‑1 (MQ=255)
                                                                                            gcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCg                                                      <  2:7229175/151‑1 (MQ=255)
                                                                                             caTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGc                                                     <  1:10305695/151‑1 (MQ=255)
                                                                                              aTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACCAACCGTCTACGCCCCCTTTCCCGATTTACGGTTCGCa                                                    <  2:2173809/151‑1 (MQ=255)
                                                                                              aTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCa                                                    >  1:4510858/1‑151 (MQ=255)
                                                                                              aTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCa                                                    >  1:6297065/1‑151 (MQ=255)
                                                                                                  aTAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaa                                                >  1:10553805/1‑151 (MQ=255)
                                                                                                    aaTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaa                                              >  1:5196821/1‑151 (MQ=255)
                                                                                                     aTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGTCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaaa                                             <  2:7030983/151‑1 (MQ=255)
                                                                                                           aTGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCg                                       <  1:8643119/151‑1 (MQ=255)
                                                                                                              tCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCAt                                    >  1:3386290/1‑151 (MQ=255)
                                                                                                               cccAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATc                                   <  2:8467438/151‑1 (MQ=255)
                                                                                                                ccAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCt                                  <  2:1221898/151‑1 (MQ=255)
                                                                                                                       cAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGc                           <  1:4015917/151‑1 (MQ=255)
                                                                                                                       cAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGc                           <  1:10704695/151‑1 (MQ=255)
                                                                                                                       cAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGc                           <  2:1631824/151‑1 (MQ=255)
                                                                                                                        aaGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCCAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGct                          <  2:525017/151‑1 (MQ=255)
                                                                                                                         aGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAATGCCCCGCATCTGCGGGGctc                         >  2:5392942/1‑151 (MQ=255)
                                                                                                                         aGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGctc                         >  1:10365698/1‑151 (MQ=255)
                                                                                                                          gTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGctct                        <  2:8137349/151‑1 (MQ=255)
                                                                                                                          gTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCATATTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGctct                        <  1:3640747/151‑1 (MQ=255)
                                                                                                                            cccGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTAc                      >  2:4209237/1‑151 (MQ=255)
                                                                                                                               ggTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGAt                   >  2:8374065/1‑151 (MQ=255)
                                                                                                                                gtgtAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATc                  >  1:7065260/1‑151 (MQ=255)
                                                                                                                                       caccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCTACTACAAACCGTATACGCCCCCTTTACCGATGTACGGTTCGAATGAAAAATCCCCGCATCTGCGGGGCTCTACGATCAACGTCa           >  2:966513/1‑151 (MQ=255)
                                                                                                                                        accaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCAc          <  1:8371998/151‑1 (MQ=255)
                                                                                                                                        accaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCAGCTGCGGGGCTCTACGATCAACGTCAc          >  2:10753027/1‑151 (MQ=255)
                                                                                                                                          caTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTGACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCg        <  2:4047372/151‑1 (MQ=255)
                                                                                                                                           atatTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGc       <  2:6293333/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCC  >  NC_008463/5513906‑5514201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑