Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4500TG32.9% 279.3 / 19.9 163intergenic (‑170/+32)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (51/43);  new base G (43/3);  total (113/50)
Fisher's exact test for biased strand distribution p-value = 1.03e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 167 total aligned reads displayed.

CGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGC  >  NC_008463/4616301‑4616600
                                                                                                                                                       |                                                                                                                                                       
cGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑Tc                                                                                                                                                        <  2:7899030/151‑1 (MQ=255)
 gAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑Tcc                                                                                                                                                       <  2:8983235/151‑1 (MQ=255)
  aaaaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATCCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCCG‑CCCGC‑GCC‑g                                                                                                                                                     >  1:10423892/1‑151 (MQ=255)
  aaaaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCcc‑gcc‑g                                                                                                                                                     >  2:5462320/1‑151 (MQ=255)
    aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑Gtt                                                                                                                                                   <  1:2551731/151‑1 (MQ=255)
    aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑Gtt                                                                                                                                                   <  2:2924607/151‑1 (MQ=255)
      cgccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGc                                                                                                                                                 <  1:125796/151‑1 (MQ=255)
      cgccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGc                                                                                                                                                 <  2:5930995/151‑1 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑GCCGC‑Gcccgtggct                                                                                                                                                >  2:1006457/1‑144 (MQ=255)
          gcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAg                                                                                                                                             <  1:5286288/151‑1 (MQ=255)
           cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAgg                                                                                                                                            <  1:610349/151‑1 (MQ=255)
            aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGcgagaga                                                                                                                                           >  2:3903791/1‑145 (MQ=255)
            aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGa                                                                                                                                           <  2:10657321/151‑1 (MQ=255)
               aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCg                                                                                                                                        <  2:4386390/151‑1 (MQ=255)
                gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCGG‑CCCGC‑GCC‑GGTTCATTTGAGCgg                                                                                                                                       >  2:7207561/1‑151 (MQ=255)
                 tccccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGa                                                                                                                                      <  2:3466001/148‑1 (MQ=255)
                 tCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGa                                                                                                                                      <  1:5616560/151‑1 (MQ=255)
                  cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑GCC‑GGTTAAAAGGAGCGGaa                                                                                                                                     >  2:4817710/1‑150 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATc                                                                                                                                    <  1:4997170/151‑1 (MQ=255)
                   aCCGTAAAGGCTGCAAAGAGTATACGCCTCGGCAAGGCCGCACACTGAGCAACTAGGCAAGCAAAATCACAGGATTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATc                                                                                                                                    <  2:709888/151‑1 (MQ=255)
                    ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCg                                                                                                                                   <  1:5766320/151‑1 (MQ=255)
                    ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCg                                                                                                                                   <  2:10767121/151‑1 (MQ=255)
                     cGTAAAGGCTGCAAAGTTTATACGCCGCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCgg                                                                                                                                  <  2:654489/151‑1 (MQ=255)
                      gtcaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GGTTGATAGGTGCGGGTCGgc                                                                                                                                 >  2:9461254/4‑151 (MQ=255)
                        aaaGGTTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCC‑CCCGC‑TCC‑GGTGCAAAGGAGCGGAACCgcgc                                                                                                                               >  2:2358288/1‑151 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCACCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCGG‑CCCGC‑ACC‑CGGTCATTGGAGCGGGTCggggg                                                                                                                               >  2:2334999/1‑148 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGgcgc                                                                                                                               <  1:6844827/151‑1 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGgcgc                                                                                                                               <  1:3048899/151‑1 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCC‑GCC‑CTTGCATAGTAGCGGaaacgcgc                                                                                                                               >  2:6109289/1‑144 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCCCCCG‑CCCGC‑TGC‑CGTGCAAAGTAGCGGATGGgcgg                                                                                                                               >  2:10445694/1‑150 (MQ=255)
                         aaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCGG‑CCCCCGCCC‑GTTGC‑TAGGGGCGGCTCGgcgcg                                                                                                                              >  2:7407035/1‑151 (MQ=255)
                         aaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCCC‑GAC‑CGTGCAAATGAGCGGATCGgcgcg                                                                                                                              >  2:3834993/1‑151 (MQ=255)
                            gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCCG‑GCCCC‑CCC‑GGTTCAGAGGAGGGGATGCGCGCGCCg                                                                                                                           >  1:5766607/1‑151 (MQ=255)
                            gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCg                                                                                                                           <  2:7038901/151‑1 (MQ=255)
                            gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCg                                                                                                                           <  1:4327760/151‑1 (MQ=255)
                            gCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCg                                                                                                                           >  2:5640390/1‑151 (MQ=255)
                             cTGCAAAGTGTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCGG‑CCCGC‑GCC‑GGGGGAAAGGTGCGGGTGCGCGCGCCgg                                                                                                                          >  1:9476207/1‑150 (MQ=255)
                                cAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCCG‑CCCGC‑ACC‑CGTGGATAGGAGCGGCTCGGCGCGCCGAcac                                                                                                                       >  1:7973928/1‑149 (MQ=255)
                                 aaaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCGG‑GCCCC‑ACC‑CGTGCGGAGGTGCGGGTGGGCgcggcgccgca                                                                                                                      >  1:3394634/1‑146 (MQ=255)
                                 aaaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCGGG‑CCCCC‑GCC‑CGTGGATATTTGCGGCTCGGGGCGCCgccgac                                                                                                                      >  1:5648292/1‑146 (MQ=255)
                                   aggttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGC‑CCCCC‑GCC‑CGTGACTAGGAGCGGAGAGGCGCGCCGACGCAca                                                                                                                    >  1:1581213/4‑150 (MQ=255)
                                      ttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑GCC‑GTTGGCTAGGCGCGGATCCGCGCCCCCACTCACAGcc                                                                                                                 >  2:8641094/1‑151 (MQ=255)
                                        ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGCGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑GCCGC‑TCC‑GGTGGATAGGAGCGGATCGGCGCGCCGCCTCACGGCCAg                                                                                                               >  2:7891390/1‑151 (MQ=255)
                                        ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCCGCCC‑GTGGCA‑AGGGGCGGCTAGGGGCGCCGACACACAGCCac                                                                                                               >  1:6079490/1‑150 (MQ=255)
                                        ataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCC‑GAC‑GGTGGATAGGGGCGGGTCGGCGCGCCGACACACAGcccc                                                                                                               >  2:4049051/1‑149 (MQ=255)
                                          aCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCCG‑GCCGG‑GGC‑GGTGCATAGGGGCGGGTACGGGCGCCGCCACACTggccacc                                                                                                             >  2:1797719/1‑145 (MQ=255)
                                            gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCCC‑GCC‑GGTGGCTTGGGGCGGGGGGGCGCGCCGGCACAcagccagccgg                                                                                                           >  1:7955510/1‑148 (MQ=255)
                                            gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCCG‑CCCCC‑GCC‑CGTGGATAGGTTCGGGTGGGCGCGCCGCCACACAGCCACCggg                                                                                                           >  1:5979676/1‑150 (MQ=255)
                                             ccTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑GCCGGGTGC‑TAGGAGCGGGTCGGCGCGCCGACACACAGCCAGCggcc                                                                                                          >  2:7361084/1‑149 (MQ=255)
                                               tCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCGC‑GCC‑GGTGCGTAGGTGCGGGTCGGGGCGCCGACGCACAGGCACCGGgaaa                                                                                                        >  2:7669099/1‑150 (MQ=255)
                                               tCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GGTGGATAGGTGCGGATCGGCGCGCGGCCGCACTGCCAGCgggatt                                                                                                        >  2:7669011/1‑147 (MQ=255)
                                                cGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TAC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAAtg                                                                                                       >  2:7647839/1‑150 (MQ=255)
                                                     aGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCCG‑CCCGC‑GCC‑GGTGCGGAGGATCGGGTCGGGGCGCCCGCACCCTGCCAGCGGAAAATCAGtt                                                                                                  >  1:10752061/1‑151 (MQ=255)
                                                       gCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCGG‑CCCGC‑GGC‑GTTGCATTGGTGCGGCTGGGCGCGCCCGCGCACAGGCCGCCGAAATTGAGGTgg                                                                                                >  2:635735/1‑151 (MQ=255)
                                                           cacaATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GGTGCCTATGTGCGGGTGGGGGCGCCGACTAACAGCCAGCGGGACATCAGTTGGgaag                                                                                            >  2:10219499/1‑149 (MQ=255)
                                                            acaATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCCC‑GCC‑CGTGCATAGGGGCGGGGCGGCGCGCCGCCACACAGCCCCCGGGAAGTAAGTTggggaga                                                                                           >  1:8950238/1‑147 (MQ=255)
                                                             caATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCGC‑GCC‑CGTGGAAAGGTGCGGGTAGGGGCGCCCACACACATCCAGCGGGAAATAAATTTGgaccac                                                                                          >  2:10559234/1‑151 (MQ=255)
                                                               aTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCC‑TCC‑CGTGGGGAGGCGCGGGGGGGCGCGCCGACGCACTGCCCGCGGGAATTCAGGTGGgacgacca                                                                                        >  2:3248727/1‑149 (MQ=255)
                                                                   gCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCCG‑CCCCC‑ACC‑CTTGGCACGGGGCCGCTCGGCGCGCCCCCACACATGCAGCGGGAAATAAGGTGGGGCGACggcggc                                                                                    >  2:2959444/1‑151 (MQ=255)
                                                                     aaCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCCC‑ACC‑CGTGGATAGGGGCGGATTGGCGCGCCGCCACACGTCCACCGGGGAATAAGTTGGGAGGACGACGGGAc                                                                                  >  2:6519488/1‑151 (MQ=255)
                                                                      accacccaagaaaAACCACCGGTTGGCCTATCGCCCCGCCTCCTGCCCCAGCCCAGCGCCCGTCCCCGCGCCGC‑CCCGC‑TCC‑GTGGCATCGGGGCTCAGCGCCGCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCAcc                                                                                 <  2:6228734/145‑1 (MQ=255)
                                                                             aagcaaAATCAAAGGTTTTCCTAAAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGG‑TCC‑GGTTGTTAGGAGGGGATGGGCGCGCCGACTCACTGGCAGGGGTGATTGGGTAGGGACGGAGACCGCAACGgcgcgc                                                                          >  2:7703009/1‑151 (MQ=255)
                                                                             aagcaaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCCG‑GCCGC‑TGC‑GATGGCGAGGACGGGAGAGGCGCGCCCACGCACAGCCAGAGCGAAATCAGTTGGGTCGCCGACGGCAACCCCgcgc                                                                          >  2:3167436/1‑151 (MQ=255)
                                                                               taaaaaTCAATGTTTGGCCTTTCGCGGGGGCTCCTTCCCCCTACCACGGGCCGGTCGCGCGCCGG‑CCCGC‑TGC‑GTTGAATTGGTGCGGTGGGGCGCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTg                                                                        <  2:2519288/149‑1 (MQ=255)
                                                                                caaAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCCTCGGCCGGTCGCGCGCCGGCCCCGT‑GCG‑GTTGC‑TAGGTGCGGATCGGCGCGCCGACTCACAGGCCGCGGGAATTGAGGTGGGTCGCCCGCGGGACCGCGGCGCTgg                                                                       >  2:5687321/1‑151 (MQ=255)
                                                                                   aaTCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCGC‑TCC‑GGGGAATAGGGGGGGGTGGGCGCCCCGACTCCCAGGCCGCGGGAAATTAGGTGGGGCGACCGCGGCACCGCCCCGGGGGgcg                                                                    >  2:10518705/1‑151 (MQ=255)
                                                                                     tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCGC‑GCC‑GGTGCAAAGGAGCGGAGCGGCGCCCCCGCGCAAAGACCCCCGCAAATCACTTTGGTCGACGACGGCAACGCCGCCCGGGgccca                                                                  >  2:2469979/1‑148 (MQ=255)
                                                                                       aaaGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCGG‑CCCGC‑GCC‑CTTGGCTAGGAGCGGGTCGGGGCGCCGCCGCACAGCCCGCGGAAATTACGTTGGGGCGGAGACGGCGCCCCCGCCGCGGgcgcggg                                                                >  2:3038509/1‑148 (MQ=255)
                                                                                          ggTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCGC‑TCC‑CGTGGATAGGAGCGGAGCGGCGCGCCGGCGCACGGCCCGCGGGGATTCAGGTGGGTCGACGACGGCACCGCCGCGCGGGGCGCGGCggg                                                             >  2:5716977/1‑151 (MQ=255)
                                                                                                ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCCG‑CCCGC‑GCC‑GGTGGAAAGGGGAGGAACGGGGGCCCGCCCCACGGCCAGCGGGCAGTGCGGGGGGTCGACGACGGCACCCCCGCGCGGGGCGCGGCgggcgggcg                                                       >  2:7524583/1‑150 (MQ=255)
                                                                                                   aTAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCC‑ACC‑GGTGCATATGAGCGGATAGGCGCCCCGACACACTTCCACCGGAAAATAAGTTGGGCCGAAGACGGCACCCCCCCGCGGGGGGCAGCGGGCAGGCCggg                                                    >  2:10765889/1‑150 (MQ=255)
                                                                                                    tAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCCC‑TCC‑CGGGGAGTGGAGCGGGTGGGCGCGCCGACACACGGCCCGCGGCAATTAAGGTGGGCCGACGACGGCCCCCCCCCGCGGGGCGCGGGGGGGTGGCCGGAg                                                   >  2:2619088/1‑151 (MQ=255)
                                                                                                          aGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCCG‑CCCCC‑GCC‑CTTTCATAGGAGCGGAGACGGGCGCCGACTCCCAGGCCGCGGAAATTCAGGTGGGGCCGCGGCCGCACCCCCGCGCGTGGGGCGGGGGGCTGGCCGGAGGCagac                                             >  2:8815911/1‑148 (MQ=255)
                                                                                                             cTCCTTGCCCAGAGCATCGGCCGGTCGCGGGCCGG‑CCCGC‑GCC‑GGTTCATATGTGCGGATCGGGGCGCCGCCACACAGCCCGCGGCAAATGATGTGGGTCGCAGCCGGCACCCCCGCGCGGGGGGCGGCGGGCGGGCCGGGGGcagccagc                                          >  2:746013/1‑151 (MQ=255)
                                                                                                                     ccAGAGCATCGGCCGGTCGCGCGCCGG‑CCCCC‑TCC‑GGTGCCTATGGGCGGATCGGGGCGCCGACGCACATCCAACGGAAATTAAGTTGGGTCGACGACCGCACCGCCGCCCTGGGCGCGGCGGGCTGGCCGGGGGCATGCAGCCGGACGcg                                  >  2:7523038/1‑150 (MQ=255)
                                                                                                                      cAGAGCAGCGGCCGCTCGCGCGCCGG‑CCCGC‑GCC‑GTGTCTTAGGTGGGTAGCGGCGCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTg                                 <  1:8810883/151‑1 (MQ=255)
                                                                                                                           cagcggcggGCCGCGCGCGGG‑CCGGC‑TCC‑GGTGCTTGGGCGGGGATGGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGc                            <  2:8804233/148‑1 (MQ=255)
                                                                                                                           caccgcccGGCCGCGCCCCGG‑CCCGC‑TCG‑GTTCAAATGTCGGGGAGGGCCGCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGc                            <  1:7202801/145‑1 (MQ=255)
                                                                                                                              cggcggGTCGCGCGCCGG‑CCCGC‑CCG‑GTTGCATCGGTGCGGCTCGGCCGCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCgg                         <  2:5348224/151‑1 (MQ=255)
                                                                                                                                    ggcgcgcgCCGC‑CCCGC‑GCC‑GTTGACGAGGCGCGGAGCGGCCCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTc                   <  1:9907257/149‑1 (MQ=255)
                                                                                                                                      cgggcgcCGC‑CCCGC‑TCG‑GTTGCTTCGGCGCGGTCCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCt                 <  2:10856271/148‑1 (MQ=255)
                                                                                                                                        cgcgcCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGc               >  2:1521889/1‑151 (MQ=255)
                                                                                                                                         gcgcCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCa              >  2:6653856/1‑151 (MQ=255)
                                                                                                                                          cgcCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCCGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa             >  1:7625556/1‑151 (MQ=255)
                                                                                                                                          cgcCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCaa             >  1:7167169/1‑151 (MQ=255)
                                                                                                                                             cGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg          >  1:9863553/1‑151 (MQ=255)
                                                                                                                                              gg‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGTAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc         >  1:5569607/1‑151 (MQ=255)
                                                                                                                                              gg‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc         >  2:2797919/1‑151 (MQ=255)
                                                                                                                                              gg‑CCCGC‑TCC‑GGTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGACGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc         >  2:4730096/1‑151 (MQ=255)
                                                                                                                                               g‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg        >  2:2616461/1‑151 (MQ=255)
                                                                                                                                                 cccGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcgc       >  2:8635219/1‑151 (MQ=255)
                                                                                                                                                  ccGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc      >  2:8459420/1‑151 (MQ=255)
                                                                                                                                                   cGC‑TCG‑TTTTCATTGGAGGGTATCGGCGCCCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa     <  2:5039384/151‑1 (MQ=255)
                                                                                                                                                   cGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa     >  1:1245946/1‑151 (MQ=255)
                                                                                                                                                   cGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCa     >  2:9598020/1‑151 (MQ=255)
                                                                                                                                                     c‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg   >  2:3519943/1‑151 (MQ=255)
                                                                                                                                                     c‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg   >  1:9329515/1‑151 (MQ=255)
                                                                                                                                                       tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc  >  2:301380/1‑151 (MQ=255)
                                                                                                                                                       tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc  >  1:10106166/1‑151 (MQ=255)
                                                                                                                                                       tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGc  >  1:8170476/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
CGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGCCGG‑CCCGC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCAGTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGC  >  NC_008463/4616301‑4616600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑