Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,051,443 T→C K359E (AAG→GAG)  PA14_RS13990 ← SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,051,4430TC100.0% 1722.5 / NA 448K359E (AAG→GAG) PA14_RS13990SfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (251/197);  total (251/197)

Only 100 of 448 total aligned reads displayed.

GACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGC  >  NC_008463/3051296‑3051590
                                                                                                                                                   |                                                                                                                                                   
gACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGaa                                                                                                                                                  >  1:8775890/1‑151 (MQ=255)
gACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGaa                                                                                                                                                  <  2:9197405/151‑1 (MQ=255)
 aCGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAag                                                                                                                                                 <  2:1965947/151‑1 (MQ=255)
   gcgcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAagag                                                                                                                                               <  2:5557651/151‑1 (MQ=255)
     gcCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCt                                                                                                                                             >  1:841216/1‑151 (MQ=255)
      ccGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCtt                                                                                                                                            >  1:6638785/1‑151 (MQ=255)
       cGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtc                                                                                                                                           <  2:3142677/151‑1 (MQ=255)
         gACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtctc                                                                                                                                         >  2:8489244/1‑151 (MQ=255)
         gACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTtctc                                                                                                                                         <  1:4533427/151‑1 (MQ=255)
          aCGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCg                                                                                                                                        >  2:1744130/1‑151 (MQ=255)
           cGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGc                                                                                                                                       >  2:6119901/1‑151 (MQ=255)
            gggCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGGGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCt                                                                                                                                      >  2:7581646/1‑151 (MQ=255)
               cgcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggc                                                                                                                                   >  1:7303753/1‑151 (MQ=255)
                gcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcg                                                                                                                                  <  1:8993262/151‑1 (MQ=255)
                gcCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTggcg                                                                                                                                  >  1:6419287/1‑151 (MQ=255)
                    ttCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCa                                                                                                                              <  1:5610505/151‑1 (MQ=255)
                      cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                                                            <  2:6841659/151‑1 (MQ=255)
                      cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                                                            >  1:1695653/1‑151 (MQ=255)
                      cAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAgg                                                                                                                            <  2:10219392/151‑1 (MQ=255)
                        ggTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCt                                                                                                                          >  1:5140425/1‑151 (MQ=255)
                         gtagtaGTCGCCGACCGCGTGGACCTCCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTg                                                                                                                         >  1:9222826/1‑151 (MQ=255)
                          tagtagTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGa                                                                                                                        >  1:9562709/1‑151 (MQ=255)
                          tagtagTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGa                                                                                                                        >  1:3456441/1‑151 (MQ=255)
                             tagtCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGGGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGAtct                                                                                                                     <  2:4096102/151‑1 (MQ=255)
                                 cGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGt                                                                                                                 <  1:2872423/151‑1 (MQ=255)
                                 cGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGt                                                                                                                 >  1:8597259/1‑151 (MQ=255)
                                  gCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTg                                                                                                                >  2:8880544/1‑151 (MQ=255)
                                   ccGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGc                                                                                                               >  1:10056868/1‑151 (MQ=255)
                                     gACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                                                             >  2:10061349/1‑151 (MQ=255)
                                     gACCGCGTGGACCGTCCAGCGTACCGGGTCTTGCAGGGTGTGGGTGCTGGCGTTGCGCCAGGGCCGGTCGAGGTTGAACTCGGCGAGCGAGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTc                                                                                                             <  1:1390707/151‑1 (MQ=255)
                                       ccGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAg                                                                                                           <  1:6004711/151‑1 (MQ=255)
                                           gTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGGTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAcc                                                                                                       <  2:3418498/151‑1 (MQ=255)
                                           gTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCAcc                                                                                                       >  2:7284463/1‑151 (MQ=255)
                                              gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGACGAGCGTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    >  2:2913025/1‑151 (MQ=255)
                                              gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    <  2:4340770/151‑1 (MQ=255)
                                              gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    <  1:5518468/151‑1 (MQ=255)
                                              gACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    >  1:364739/1‑151 (MQ=255)
                                              gACCTGCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGGTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGTCCTGGCTGATCTCGGTGCTCAGCACCTTg                                                                                                    <  1:4641063/151‑1 (MQ=37)
                                                 cTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGcc                                                                                                 <  2:7434052/151‑1 (MQ=255)
                                                  ttCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCt                                                                                                <  1:7177895/151‑1 (MQ=255)
                                                    ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              >  2:9470554/1‑151 (MQ=255)
                                                    ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              <  2:6708878/151‑1 (MQ=255)
                                                    ccAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCg                                                                                              <  1:2251751/151‑1 (MQ=255)
                                                      aGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGc                                                                                            <  2:6480270/151‑1 (MQ=255)
                                                         gTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCAc                                                                                         >  2:5403618/1‑151 (MQ=255)
                                                          tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                                                                        <  1:10410609/151‑1 (MQ=255)
                                                          tACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACg                                                                                        >  1:9973964/1‑151 (MQ=255)
                                                                gTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGACCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGTAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATc                                                                                  >  2:1227025/1‑151 (MQ=255)
                                                                   gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               >  2:9093263/1‑151 (MQ=255)
                                                                   gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               >  1:4178463/1‑151 (MQ=255)
                                                                   gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               >  2:5943846/1‑151 (MQ=255)
                                                                   gTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAg                                                                               >  1:9931315/1‑151 (MQ=255)
                                                                    tGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAgg                                                                              >  1:10318776/1‑151 (MQ=255)
                                                                     gCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgc                                                                             <  1:4797656/151‑1 (MQ=255)
                                                                     gCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgc                                                                             <  2:8350015/151‑1 (MQ=255)
                                                                       aGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGgcgc                                                                           >  1:7042648/1‑151 (MQ=255)
                                                                        gggTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGcgcg                                                                          <  2:448620/151‑1 (MQ=255)
                                                                         ggTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGt                                                                         <  1:10804352/151‑1 (MQ=255)
                                                                               ggTGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTgccgcc                                                                   <  2:8572142/151‑1 (MQ=255)
                                                                                 tGCGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGa                                                                 >  2:8939157/1‑151 (MQ=255)
                                                                                   cGGGCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACt                                                               >  1:4362715/1‑151 (MQ=255)
                                                                                    gggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTc                                                              >  1:3984832/1‑151 (MQ=255)
                                                                                    gggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTc                                                              >  1:3065789/1‑151 (MQ=255)
                                                                                     ggCGTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCg                                                             <  1:6254015/151‑1 (MQ=255)
                                                                                        gTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCg                                                          >  2:10383736/1‑151 (MQ=255)
                                                                                        gTTGCGCCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCg                                                          >  1:3220887/1‑151 (MQ=255)
                                                                                             gcCAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAc                                                     <  2:8028738/151‑1 (MQ=255)
                                                                                              ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc                                                    <  1:3895899/151‑1 (MQ=255)
                                                                                              ccAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCAcc                                                    >  2:1451302/1‑151 (MQ=255)
                                                                                               cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                                                   >  2:7204197/1‑151 (MQ=255)
                                                                                               cAGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCg                                                   >  1:5400358/1‑151 (MQ=255)
                                                                                                aGTGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCgg                                                  >  2:2880115/1‑151 (MQ=255)
                                                                                                  tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  1:7728716/151‑1 (MQ=255)
                                                                                                  tGCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  1:8168640/151‑1 (MQ=255)
                                                                                                  ggCCGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGcc                                                <  2:3923522/150‑1 (MQ=255)
                                                                                                     cGGTCGAGGTTGAACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcg                                             >  1:6583144/1‑151 (MQ=255)
                                                                                                       gtcgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCcgcgcg                                           >  1:2355248/4‑151 (MQ=255)
                                                                                                         cgaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGc                                         >  1:7972216/2‑151 (MQ=255)
                                                                                                          gaggttgaACTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGcc                                        <  1:1951709/151‑1 (MQ=255)
                                                                                                                  aCTCGGCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTtcgtc                                >  1:6062764/1‑151 (MQ=255)
                                                                                                                     cggCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAg                             <  1:7451116/150‑1 (MQ=255)
                                                                                                                     cggCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAg                             <  2:10773293/150‑1 (MQ=255)
                                                                                                                     cggCGAGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAg                             <  2:4024940/150‑1 (MQ=255)
                                                                                                                          aGCGTGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCt                        <  1:853456/151‑1 (MQ=255)
                                                                                                                              tGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTggcc                    <  2:5645793/151‑1 (MQ=255)
                                                                                                                              tGGCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTggcc                    >  2:6280705/1‑151 (MQ=255)
                                                                                                                               ggCGCGGCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTggccg                   >  2:5253047/1‑151 (MQ=255)
                                                                                                                                   cgGCTGCCGGCCAGCTCGAAGAGCTGCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCGACTGCGGCGCGGACCTggccgggcg               >  2:432349/1‑148 (MQ=38)
                                                                                                                                    ggCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCtt              <  2:6154604/151‑1 (MQ=255)
                                                                                                                                    ggCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCtt              <  2:9586776/151‑1 (MQ=255)
                                                                                                                                     gCTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTtc             <  1:2659472/151‑1 (MQ=255)
                                                                                                                                      cTGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTtct            >  2:4841487/1‑151 (MQ=255)
                                                                                                                                       tGCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTtctc           >  2:10630443/1‑151 (MQ=255)
                                                                                                                                        gCCGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTcc          >  1:1842318/1‑151 (MQ=255)
                                                                                                                                         ccGGCCATCTCGCAGAGCTTCTCGCTGGCGGCCAGGCTGAGCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCca         <  2:4699408/151‑1 (MQ=255)
                                                                                                                                         ccGGCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCCCCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCca         >  1:5913596/1‑151 (MQ=255)
                                                                                                                                           ggCCAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCcagc       <  2:7005530/151‑1 (MQ=255)
                                                                                                                                             ccAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCcagcag     >  1:787467/1‑151 (MQ=255)
                                                                                                                                              cAGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCCCCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAgg    >  2:9253464/1‑151 (MQ=255)
                                                                                                                                               aGCTCGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAggg   >  1:2861882/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACGCGCCGGACGGGCGCCGTTCAGGTAGTAGTCGCCGACCGCGTGGACCTTCCAGCGTACCGGGTCGTGCAGGGTGTGGGTGCGGGCGTTGCGCCAGTGCCGGTTGAGGTTGAACTTGGCGAGCGTGGCGCGGCTGCCGGCCAGCTTGAAGAGCTTCTCGCTGGCGGCCAGGCTGATCTCGGTGCTCAGCACCTTGGCCTCGGCCACGGCGATCGAGGCGCGTGCCGCCGACTCGGCGTCCACCGGCCGCGCGGCCACTTCGTCGAGGACCTGGCCGGCCTTCTCCAGCAGGGC  >  NC_008463/3051296‑3051590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑