Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,356,495 G→C intergenic (+22/+49) PA14_RS15330 → / ← PA14_RS31420 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,356,4950GC99.8% 1560.1 / NA 411intergenic (+22/+49)PA14_RS15330/PA14_RS31420hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (224/186);  total (225/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 423 total aligned reads displayed.

CGCCCGCGAGAAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGGGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCGGGAAA  >  NC_008463/3356347‑3356642
                                                                                                                                                    |                                                                                                                                                   
 gcCCGCGAGAAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGACTAAGGTTCTCCTCCGGCAAAAGGGcgcg                                                                                                                                                  >  1:4315236/1‑151 (MQ=255)
      cgAGAAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTcc                                                                                                                                             >  1:2469443/1‑151 (MQ=255)
       gagaAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCg                                                                                                                                            >  1:5561676/1‑151 (MQ=255)
       gagaAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCg                                                                                                                                            <  2:7473688/151‑1 (MQ=255)
        agaAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTGCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGCATAAGGTTCTCCTCCGGCAAATGGGCGCGGCTCCGa                                                                                                                                           >  2:6652650/1‑151 (MQ=255)
          aaGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATa                                                                                                                                         >  2:6908702/1‑151 (MQ=255)
          aaGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATa                                                                                                                                         <  1:4115038/151‑1 (MQ=255)
               ggCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGc                                                                                                                                    <  1:6603754/151‑1 (MQ=255)
                   cAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCcgc                                                                                                                                >  1:6220567/1‑151 (MQ=255)
                       tCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGccc                                                                                                                            <  2:2768903/151‑1 (MQ=255)
                          tCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCttt                                                                                                                         >  2:7057343/1‑151 (MQ=255)
                           ccaGGGGTTCAACGAGACCGTGCAGTTCACTACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCtttt                                                                                                                        <  2:5514127/149‑1 (MQ=255)
                           cAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCtttt                                                                                                                        >  1:9885911/1‑151 (MQ=255)
                             aGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTc                                                                                                                      >  1:4681648/1‑151 (MQ=255)
                             aGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTc                                                                                                                      <  2:6336182/151‑1 (MQ=255)
                             aGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCAGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTc                                                                                                                      <  1:2306445/151‑1 (MQ=255)
                              gggTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCa                                                                                                                     >  1:71036/1‑151 (MQ=255)
                                  ttCAACGAGACCGTGCAGTTCAAGACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGGTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCg                                                                                                                 <  1:1791272/151‑1 (MQ=255)
                                   tCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGc                                                                                                                >  1:7311335/1‑151 (MQ=255)
                                     aaCGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGccc                                                                                                              >  1:2136652/1‑151 (MQ=255)
                                      aCGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCa                                                                                                             >  1:10023248/1‑151 (MQ=255)
                                      aCGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCa                                                                                                             >  1:9006214/1‑151 (MQ=255)
                                       cGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAg                                                                                                            <  2:8823297/151‑1 (MQ=255)
                                        gaggCCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGc                                                                                                           >  1:9424061/1‑151 (MQ=255)
                                         agaCCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGcc                                                                                                          >  1:5010623/1‑151 (MQ=255)
                                           aCCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCcgc                                                                                                        >  1:9336133/1‑151 (MQ=255)
                                            ccGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCcgcg                                                                                                       <  1:1426696/151‑1 (MQ=255)
                                            ccGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGGTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTATTTCATTCGCCCAGCcgcg                                                                                                       <  2:839139/151‑1 (MQ=255)
                                              gTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGcc                                                                                                     >  1:9962059/1‑151 (MQ=255)
                                              gTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGcc                                                                                                     <  1:9262903/151‑1 (MQ=255)
                                              gTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGcc                                                                                                     >  1:4517665/1‑151 (MQ=255)
                                               tGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGccc                                                                                                    <  1:7572845/151‑1 (MQ=255)
                                               tGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTGCATTCGCCCAGCCGCGccc                                                                                                    >  1:6929045/1‑151 (MQ=255)
                                                     tCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGCGCACAGCCGAACTGGACTAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCATTGTCAGTCGCCCAGCCGCGCCCGGCCGc                                                                                              >  2:3405220/1‑151 (MQ=255)
                                                        aTACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCg                                                                                           >  1:6141531/1‑151 (MQ=255)
                                                         tACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGc                                                                                          >  2:2151155/1‑151 (MQ=255)
                                                          aCCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTGTTCATTCGCCCAGCCGCGCCCGGCCGCTCGcc                                                                                         >  1:9835480/1‑151 (MQ=255)
                                                            cGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCt                                                                                       >  2:6502117/1‑151 (MQ=255)
                                                            cGCTCTGCACGCCATCAGTCATACCGGGGTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCt                                                                                       <  2:4889110/151‑1 (MQ=255)
                                                             gCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTc                                                                                      >  1:7987334/1‑151 (MQ=255)
                                                                ctGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCggg                                                                                   >  2:3594751/1‑151 (MQ=255)
                                                                 tGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGa                                                                                  >  1:10471065/1‑151 (MQ=255)
                                                                  gCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGAc                                                                                 <  2:10739742/151‑1 (MQ=255)
                                                                    aCGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGAcgc                                                                               >  2:5679024/1‑151 (MQ=255)
                                                                    aCGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGAcgc                                                                               >  1:10817936/1‑151 (MQ=255)
                                                                      gCCATCAGTCATACCGGGTTGACACGCCAGGCAGAGAGCACAGCCGAACGGGACTACGGTTCGCCTCCGGCAAAAGGGCGCGGCTCCGCGACTGGCCCGCGCCCTTTTTCAGTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCgg                                                                             >  2:9131639/1‑151 (MQ=255)
                                                                        cATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTg                                                                           <  2:3209531/151‑1 (MQ=255)
                                                                             gTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTgccgcc                                                                      >  2:5221516/1‑151 (MQ=255)
                                                                                 tACCGGGTTGAAACGCCAGGCAGAGAGCACAGCCGACCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGcc                                                                  <  2:8316309/151‑1 (MQ=255)
                                                                                  aCCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTATTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCa                                                                 >  2:1429389/1‑151 (MQ=255)
                                                                                      ggTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTc                                                             >  2:4726561/1‑151 (MQ=255)
                                                                                          gAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCg                                                         >  1:1175851/1‑151 (MQ=255)
                                                                                             aCGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTg                                                      >  1:1588300/1‑151 (MQ=255)
                                                                                               gCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTgcg                                                    <  2:1778406/151‑1 (MQ=255)
                                                                                                ccAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTgcgc                                                   <  2:5749112/151‑1 (MQ=255)
                                                                                                   ggAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCaga                                                <  2:8548851/151‑1 (MQ=255)
                                                                                                         gagCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGtt                                          >  2:7794319/1‑151 (MQ=255)
                                                                                                         gagCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGtt                                          <  2:9632141/151‑1 (MQ=255)
                                                                                                         gagCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCACCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGtt                                          <  2:906247/151‑1 (MQ=255)
                                                                                                          agCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTtg                                         >  1:3384556/1‑151 (MQ=255)
                                                                                                           gCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTtgt                                        <  2:9381811/151‑1 (MQ=255)
                                                                                                             acaGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTgc                                      <  1:4187684/151‑1 (MQ=255)
                                                                                                               aGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTgcgc                                    <  1:8249588/151‑1 (MQ=255)
                                                                                                               aGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTgcgc                                    >  2:4031240/1‑151 (MQ=255)
                                                                                                                 ccGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCg                                  >  2:5426655/1‑151 (MQ=255)
                                                                                                                  cGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCgg                                 <  2:2494637/151‑1 (MQ=255)
                                                                                                                  cGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCgg                                 <  1:9684362/151‑1 (MQ=255)
                                                                                                                    aaCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGAc                               >  2:3706543/1‑151 (MQ=255)
                                                                                                                    aaCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGAc                               >  1:671614/1‑151 (MQ=255)
                                                                                                                    aaCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGAGACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGAc                               <  2:5023189/151‑1 (MQ=255)
                                                                                                                     aCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACa                              >  1:3160708/1‑151 (MQ=255)
                                                                                                                     aCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACa                              >  1:9213582/1‑151 (MQ=255)
                                                                                                                     aCTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACa                              <  2:7529860/151‑1 (MQ=255)
                                                                                                                      cTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATCCTGGCCCGCGCCCATTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAg                             <  2:7410681/151‑1 (MQ=255)
                                                                                                                      cTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAg                             >  2:1118600/1‑151 (MQ=255)
                                                                                                                      cTGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAg                             >  2:6054798/1‑151 (MQ=255)
                                                                                                                       tGGAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCCGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAgc                            >  2:10349891/1‑151 (MQ=255)
                                                                                                                         gAATAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAgcgc                          <  1:5389822/151‑1 (MQ=255)
                                                                                                                          aaTAAGGTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGcc                         >  1:9541587/1‑151 (MQ=255)
                                                                                                                              cggTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCa                     <  2:2289899/150‑1 (MQ=255)
                                                                                                                               ggTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAg                    <  1:86048/151‑1 (MQ=255)
                                                                                                                                gTTCTCCTCCGGCAATAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGGTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAgg                   >  2:4395719/1‑151 (MQ=255)
                                                                                                                                gTTCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAgg                   >  1:5634885/1‑151 (MQ=255)
                                                                                                                                 ttCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGgc                  <  2:10547182/151‑1 (MQ=255)
                                                                                                                                 ttCTCCTCCGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGgc                  >  1:5799155/1‑151 (MQ=255)
                                                                                                                                 ttCTCCTCCGGCAAAAGGGCGCGGCTCCGAGACTGGCCCGCGCCCATTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGgc                  <  2:4928989/151‑1 (MQ=255)
                                                                                                                                    tcctccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCa               <  1:3485692/151‑1 (MQ=255)
                                                                                                                                     cctccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCAc              <  1:7620538/151‑1 (MQ=255)
                                                                                                                                     cctccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCAc              <  1:1913506/151‑1 (MQ=255)
                                                                                                                                      ctccGGCAAAAGGGCGCGGCTCCGATCCTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCGCGGGCCGCGGTGCCGCCGGCCCGTTCACCGGGGCGCAGCGCTGTTGTGCGCCGGACCGCGCCGGCTGGCGcccc             >  2:8636948/1‑151 (MQ=255)
                                                                                                                                      ctccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCGCCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCAcc             >  2:8929493/1‑151 (MQ=255)
                                                                                                                                       tccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCAccc            >  1:10301027/1‑151 (MQ=255)
                                                                                                                                        ccGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCAGTCGCCCCGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTCGTGCGCCGGCCAGCGCCGGCAGGCGCACCCa           <  2:8232706/151‑1 (MQ=255)
                                                                                                                                         cGGCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCAt          <  1:8116159/151‑1 (MQ=255)
                                                                                                                                           gCAAAAGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTc        <  2:4154425/151‑1 (MQ=255)
                                                                                                                                             aaaaGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGGCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCCGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCgg      >  2:3024674/1‑151 (MQ=255)
                                                                                                                                              aaaGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCggg     >  2:7983806/1‑151 (MQ=255)
                                                                                                                                              aaaGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCggg     >  2:1255784/1‑151 (MQ=255)
                                                                                                                                               aaGGGCGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCGGGa    <  2:1689783/151‑1 (MQ=255)
                                                                                                                                                 gggcgCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGGGCCGCCGGCCAGTTCACCGGTGCGCTGAGCCGGTGTGCGCCGGACAGCGCCGGCAGGCGCACCCCTGCgggcca  >  1:7876669/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCGCGAGAAGCTGGCGCAGATCGTCAAGGGTTTCAACGAGACCGTGCAGTTCAATACCGCTCTGCACGCCATCAGTCATACCGGGTTGAAACGCCAGGAAGAGAGCACAGCCGAACTGGAATAAGGTTCTCCTCCGGCAAAAGGGGGCGGCTCCGATACTGGCCCGCGCCCTTTTTCATTCGCCCAGCCGCGCCCGGCCGCTCGCCCTCGGGACGCGGTGCCGCCGGCCAGTTCACCGGTGCGCAGAGCAGTTGTGCGCCGGACAGCGCCGGCAGGCGCACCCATTCGGGAAA  >  NC_008463/3356347‑3356642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑