Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,373,667 G→C V352V (GTC→GTG)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,373,6670GC99.4% 1794.3 / NA 474V352V (GTC→GTG) PA14_RS15385aminotransferase class V‑fold PLP‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base C (194/277);  total (197/277)
Fisher's exact test for biased strand distribution p-value = 7.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814
                                                                                                                                                    |                                                                                                                                                   
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   <  2:3686381/151‑1 (MQ=255)
 aGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACg                                                                                                                                                  <  1:6312845/151‑1 (MQ=255)
 aGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACg                                                                                                                                                  >  1:537575/1‑151 (MQ=255)
    gCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTg                                                                                                                                               <  1:3758276/151‑1 (MQ=255)
    gCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTg                                                                                                                                               <  2:5403635/151‑1 (MQ=255)
      gggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCg                                                                                                                                             >  2:7396222/1‑151 (MQ=255)
      gggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCg                                                                                                                                             >  2:6824472/1‑151 (MQ=255)
         tCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGcc                                                                                                                                          <  1:2565000/151‑1 (MQ=255)
           gAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAg                                                                                                                                        >  2:7890650/1‑151 (MQ=255)
           gAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAg                                                                                                                                        >  2:3890422/1‑151 (MQ=255)
             gccggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAgcg                                                                                                                                      <  2:2702147/151‑1 (MQ=255)
                ggccgCTCAGCCTTCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGAGGGTGAAGCGACCGAGGCTCAGGCGCACGCTGCGGCCTGCGCTg                                                                                                                                   >  2:7202197/1‑151 (MQ=255)
                ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTg                                                                                                                                   >  1:2213252/1‑151 (MQ=255)
                ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTg                                                                                                                                   <  1:3442894/151‑1 (MQ=255)
                ggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTg                                                                                                                                   >  1:1323650/1‑151 (MQ=255)
                  ccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggc                                                                                                                                 <  2:8294584/151‑1 (MQ=255)
                  ccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggc                                                                                                                                 >  2:1910557/1‑151 (MQ=255)
                   cgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcg                                                                                                                                <  2:8455379/151‑1 (MQ=255)
                   cgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcg                                                                                                                                >  2:5559656/1‑151 (MQ=255)
                    gCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcgg                                                                                                                               >  1:9655088/1‑151 (MQ=255)
                    gCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcgg                                                                                                                               <  2:1651324/151‑1 (MQ=255)
                    gCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCCCCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcgg                                                                                                                               >  1:2652004/1‑151 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             <  2:3040551/151‑1 (MQ=255)
                         gCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCg                                                                                                                          >  2:6443459/1‑151 (MQ=255)
                          ccTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGt                                                                                                                         >  2:4667352/1‑151 (MQ=255)
                                cTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGCGGCGCAGGCGCACGCTGCGGCCCGCGCTGGCGGCATCGTGGCCGa                                                                                                                   >  2:6641922/1‑151 (MQ=255)
                                        cccGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCgaga                                                                                                           <  1:4171455/151‑1 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGCGAAGCa                                                                                                       >  2:275224/1‑151 (MQ=255)
                                            aGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCa                                                                                                       >  2:1535340/1‑151 (MQ=255)
                                               aaCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGt                                                                                                    <  1:2046094/151‑1 (MQ=255)
                                                aCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCCCGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTg                                                                                                   >  2:3340670/1‑151 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGa                                                                                                <  1:10045397/151‑1 (MQ=255)
                                                          cGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgcc                                                                                         >  2:2363038/1‑151 (MQ=255)
                                                          cGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgcc                                                                                         <  1:10706022/151‑1 (MQ=255)
                                                                 gcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGTCCTCGCGGGTGTAGCGACCTAGGCGCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCCCGTGGGACGGCGCCGGCcgcg                                                                                  >  2:5064917/1‑151 (MQ=255)
                                                                 gcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCcgcg                                                                                  >  2:6060526/1‑151 (MQ=255)
                                                                 gcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCcgcg                                                                                  <  1:4551522/151‑1 (MQ=255)
                                                                  cgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCgcgc                                                                                 >  1:3004199/1‑151 (MQ=255)
                                                                   gcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGcc                                                                                >  2:5649125/1‑151 (MQ=255)
                                                                   gcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGcc                                                                                <  2:5953041/151‑1 (MQ=255)
                                                                   gcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGcc                                                                                >  1:145675/1‑151 (MQ=255)
                                                                      aCTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                             >  2:10854831/1‑151 (MQ=255)
                                                                              ccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGc                                                                     <  1:8640905/151‑1 (MQ=255)
                                                                               cgcgGCCTTGAGGGCGGCCCCCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCAGCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcc                                                                    <  2:9892432/151‑1 (MQ=255)
                                                                               cgcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcc                                                                    <  1:3328870/151‑1 (MQ=255)
                                                                               cgcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGcc                                                                    <  1:9146397/151‑1 (MQ=255)
                                                                                gcgGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCg                                                                   >  2:7049734/1‑151 (MQ=255)
                                                                                  ggCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAg                                                                 <  1:473023/151‑1 (MQ=255)
                                                                                   gCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAgg                                                                <  2:7290846/151‑1 (MQ=255)
                                                                                    ccTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAggg                                                               >  2:136924/1‑150 (MQ=255)
                                                                                         aGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTgg                                                          >  1:5037890/1‑151 (MQ=255)
                                                                                              ggcCACCGCCGCGTCGACGTCGGCCTCCCGGGTGTAGCGACCGAGGGTCTGGGGCACGATGCGGCCAGCGCTGGCGGAATCGAGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACAcc                                                     >  2:2130829/1‑151 (MQ=255)
                                                                                               gcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCg                                                    <  1:10447302/151‑1 (MQ=255)
                                                                                                ccACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGc                                                   <  2:4073872/151‑1 (MQ=255)
                                                                                                 cACCGCCGCGTCGACTTCGGCCTCGCGGGTTTAGCGACCGTGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGcc                                                  <  2:9162344/151‑1 (MQ=255)
                                                                                                  aCCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCa                                                 <  1:2322840/151‑1 (MQ=255)
                                                                                                   ccgccgCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAg                                                <  2:10528604/151‑1 (MQ=255)
                                                                                                    cgccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAgg                                               <  1:7724838/151‑1 (MQ=255)
                                                                                                    cgccgcGGCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAgg                                               <  2:9028885/151‑1 (MQ=255)
                                                                                                      ccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCt                                             <  1:4795991/151‑1 (MQ=255)
                                                                                                       cgcgTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTg                                            >  1:6442930/1‑151 (MQ=255)
                                                                                                           tCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACt                                        <  1:9043297/151‑1 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCCCGTGGGTCGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  1:5194984/1‑151 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGGAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGACGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  2:772742/1‑151 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   <  1:4625915/151‑1 (MQ=255)
                                                                                                                    gCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                               <  1:1857690/151‑1 (MQ=255)
                                                                                                                      cTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGAGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                             >  2:4817136/1‑151 (MQ=255)
                                                                                                                       tCGCGGGTGGAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                            <  2:8867709/151‑1 (MQ=255)
                                                                                                                        cgcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                           <  1:3163030/151‑1 (MQ=255)
                                                                                                                        cgcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                           <  1:6547893/151‑1 (MQ=255)
                                                                                                                         gcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggggg                          <  1:1127595/151‑1 (MQ=255)
                                                                                                                          cgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGa                         >  1:7426584/1‑151 (MQ=255)
                                                                                                                           gggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        >  2:4206624/1‑151 (MQ=255)
                                                                                                                           gggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCGGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        <  2:9338432/151‑1 (MQ=255)
                                                                                                                            ggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                       <  2:57839/151‑1 (MQ=255)
                                                                                                                            ggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                       <  1:27465/151‑1 (MQ=255)
                                                                                                                            ggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACa                       >  1:7951840/1‑151 (MQ=255)
                                                                                                                              tgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaaa                     <  1:3953646/151‑1 (MQ=255)
                                                                                                                              tgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaaa                     <  1:7136601/151‑1 (MQ=255)
                                                                                                                               gtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                    <  2:9655088/151‑1 (MQ=255)
                                                                                                                               gtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                    <  2:9768015/151‑1 (MQ=255)
                                                                                                                               gtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAg                    <  2:10723089/151‑1 (MQ=255)
                                                                                                                                tAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAgg                   <  1:8381938/151‑1 (MQ=255)
                                                                                                                                tAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGCGGTGCTGGACACCGCGAGGCTGGACTCCAGTGCCTGGGGGACatcgg                   >  2:10089549/1‑147 (MQ=255)
                                                                                                                                  gCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGcc                 <  1:5825268/151‑1 (MQ=255)
                                                                                                                                  gCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGcc                 <  1:3856176/151‑1 (MQ=255)
                                                                                                                                  gCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGcc                 <  1:6860542/151‑1 (MQ=255)
                                                                                                                                  gCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGcc                 >  2:4396902/1‑151 (MQ=255)
                                                                                                                                   cGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCg                <  1:10141141/151‑1 (MQ=255)
                                                                                                                                    gACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCgg               >  2:10566600/1‑151 (MQ=255)
                                                                                                                                     aCCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCggg              <  2:3482593/151‑1 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           <  2:8243717/151‑1 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           <  2:10337993/151‑1 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           >  1:9355118/1‑151 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           >  1:1753457/1‑151 (MQ=255)
                                                                                                                                            cTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGa       <  1:5940715/151‑1 (MQ=255)
                                                                                                                                             tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGaa      <  1:8251726/151‑1 (MQ=255)
                                                                                                                                               aGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACATTGGCCGGGGTGTCGAAgg    >  1:1607943/1‑151 (MQ=255)
                                                                                                                                               aGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAgg    <  2:1016006/151‑1 (MQ=255)
                                                                                                                                               aGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAgg    >  1:9795361/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑