BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,387,884	T→C	F48L (TTC→CTC)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,387,884	0	T	C	99.8%	1581.8 / NA	414	F48L (TTC→CTC)	cynS	cyanase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (202/211); minor base G (1/0); total (203/211)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

TCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACC  >  NC_008463/3387736‑3388028

tCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTc                                                                                                                                                >  1:1093260/1‑151 (MQ=255)

   tCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGc                                                                                                                                             >  1:8122727/1‑151 (MQ=255)

       aagCAACACAGCCAAGGGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGc                                                                                                                                         <  2:1820385/149‑1 (MQ=255)

         gCAACACAGCCAAGTGTCCCCCCCCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCAt                                                                                                                                       <  1:5596291/151‑1 (MQ=255)

          cAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATg                                                                                                                                      >  2:3252723/1‑151 (MQ=255)

             cacaGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGccc                                                                                                                                   >  1:7468260/1‑151 (MQ=255)

                aGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGc                                                                                                                                >  2:8138577/1‑151 (MQ=255)

                aGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGc                                                                                                                                >  1:9417491/1‑151 (MQ=255)

                 gCCAAGTGTCCCCCACCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACACGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGcc                                                                                                                               <  2:2085980/151‑1 (MQ=255)

                  ccAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCa                                                                                                                              <  2:4130784/151‑1 (MQ=255)

                   cAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                             >  1:1237803/1‑151 (MQ=255)

                   cAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCag                                                                                                                             >  2:6828831/1‑151 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           >  1:3420445/1‑151 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           >  1:5124064/1‑151 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           <  1:4768600/151‑1 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           <  2:1906732/151‑1 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           >  1:10305775/1‑151 (MQ=255)

                     aGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGACGCCCTGCTCGGCCAGCATGCCCTGCCagag                                                                                                                           <  2:81200/151‑1 (MQ=255)

                      gtgtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAgg                                                                                                                          <  1:9110369/151‑1 (MQ=255)

                      gtgtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAgg                                                                                                                          >  1:5399439/1‑151 (MQ=255)

                      gtgtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCAGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAgg                                                                                                                          <  2:5179823/151‑1 (MQ=255)

                        gtCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcg                                                                                                                        <  2:9040429/151‑1 (MQ=255)

                            cccAACGCGCGCCAGCAACTGTCCGTGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCGTGGCAGGATCTGGCCGACGGCACCGGTCTCTCCATGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCATGCCCGAggcggcgg                                                                                                                    >  2:9431871/1‑151 (MQ=255)

                             ccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggcggc                                                                                                                   >  1:8300447/1‑151 (MQ=255)

                             ccAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAggcggcggc                                                                                                                   <  1:7931355/151‑1 (MQ=255)

                              cAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGgcggcggcg                                                                                                                  <  1:1945987/151‑1 (MQ=255)

                               aaCGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGgcgc                                                                                                                 >  1:7620074/1‑151 (MQ=255)

                                   cgcgcCAGCACCTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCaa                                                                                                             <  2:10555144/151‑1 (MQ=255)

                                      gcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGt                                                                                                          >  2:10810893/1‑151 (MQ=255)

                                      gcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGt                                                                                                          >  2:5497965/1‑151 (MQ=255)

                                      gcCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGt                                                                                                          >  2:9280096/1‑151 (MQ=255)

                                           cAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTcgccg                                                                                                     >  1:2482555/1‑151 (MQ=255)

                                               tGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCa                                                                                                 >  2:8833809/1‑151 (MQ=255)

                                                    gagaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCg                                                                                            >  2:5228327/1‑151 (MQ=255)

                                                     agaCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCgg                                                                                           >  1:8468263/1‑151 (MQ=255)

                                                       aCCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGcc                                                                                         <  1:6586036/151‑2 (MQ=255)

                                                       aCCGTGGTCCAGCACAACGCCCGCCTGGGCCTGTCCTCGCAGGATCTGGCCGACGGCACCCGTCCCCCCCGGACCTTCGTCACCGCCCCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGcc                                                                                         <  2:3921973/151‑2 (MQ=255)

                                                         cGTGGTCCTGAACAAGGCCCGCCTGGGCCGGTCCTGGCAGGTTCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                       <  2:5993473/151‑1 (MQ=255)

                                                          gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                      <  2:8393448/151‑1 (MQ=255)

                                                          gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                      >  2:577855/1‑151 (MQ=255)

                                                          gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCGGGCCGACGGCACCGGTCTCGCCCTGACCTGCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTgg                                                                                      <  2:7903915/151‑1 (MQ=255)

                                                           tGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGga                                                                                     >  1:7985247/1‑151 (MQ=255)

                                                           tGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGga                                                                                     <  2:7631034/151‑1 (MQ=255)

                                                             gTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacg                                                                                   <  1:9329653/151‑1 (MQ=255)

                                                                cTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacg                                                                                <  2:6960101/151‑1 (MQ=255)

                                                                cTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacg                                                                                >  1:6135523/1‑151 (MQ=255)

                                                                cTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacg                                                                                <  1:9597235/151‑1 (MQ=255)

                                                                  gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacgac                                                                              >  2:3647144/1‑151 (MQ=255)

                                                                   aaCCAGGCCCGCCTGGGCCGGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGacgacgacg                                                                             <  2:4373414/151‑1 (MQ=255)

                                                                     cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgcg                                                                           <  2:2185888/151‑1 (MQ=255)

                                                                      aaGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCgg                                                                          >  1:9956759/1‑151 (MQ=255)

                                                                       aGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGt                                                                         <  1:2065041/151‑1 (MQ=255)

                                                                          cccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCt                                                                      <  2:10347596/151‑1 (MQ=255)

                                                                          cccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCt                                                                      <  1:6435652/151‑1 (MQ=255)

                                                                           ccGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTc                                                                     <  2:7005568/151‑1 (MQ=255)

                                                                            cGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTcc                                                                    <  2:3772010/151‑1 (MQ=255)

                                                                            cGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCGTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTcc                                                                    <  2:799025/151‑1 (MQ=255)

                                                                             gCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCct                                                                   >  1:6902279/1‑151 (MQ=255)

                                                                             gCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCct                                                                   >  2:8544634/1‑151 (MQ=255)

                                                                               cTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgc                                                                 >  1:10024558/1‑151 (MQ=255)

                                                                                tGGGCCTGTCCTGGCAGGAGCTGGCCGACGGCACCGGTCTCGCCCTGTCCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCTGAGGCGGCGGCGCGCTAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCctgct                                                                >  1:4023013/1‑151 (MQ=255)

                                                                                   gCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCa                                                             <  1:4168710/151‑1 (MQ=255)

                                                                                   gCCTGTCCTGGCAGGAGCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCCGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTGGGCCTGGACGACGACGCGGTGCTCCtgctgct                                                             >  2:5411897/1‑150 (MQ=255)

                                                                                    ccTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCaa                                                            <  1:7228376/151‑1 (MQ=255)

                                                                                      tGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                          <  1:304675/151‑1 (MQ=255)

                                                                                         ccTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCAt                                                       >  2:10241715/1‑151 (MQ=255)

                                                                                           tGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATcc                                                     >  1:599122/1‑151 (MQ=255)

                                                                                            ggCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCCGCATGCCCTGCCAGAGGCGGCGGCGCGCCAGGTCGCCGCCCCACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCACAGCGTccg                                                    >  2:5715008/1‑150 (MQ=255)

                                                                                            ggCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATccc                                                    >  2:2681673/1‑151 (MQ=255)

                                                                                            ggCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATccc                                                    <  1:2534980/151‑1 (MQ=255)

                                                                                             gCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCg                                                   >  1:1319966/1‑151 (MQ=255)

                                                                                              cAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGc                                                  >  2:6421996/1‑151 (MQ=255)

                                                                                                 gATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgc                                               <  2:7349833/151‑1 (MQ=255)

                                                                                                    cTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGcgcg                                            <  1:10590255/151‑1 (MQ=255)

                                                                                                     tGGCCGACGGCACCGGTCTCGCCCTGACCGTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                           <  2:4237792/151‑1 (MQ=255)

                                                                                                         cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagc                                       <  1:5732341/151‑1 (MQ=255)

                                                                                                          gACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagct                                      >  2:4763412/1‑150 (MQ=255)

                                                                                                          gACGGCACCGGTCTCGCCCTGACCTACGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcagca                                      <  2:6092135/151‑1 (MQ=255)

                                                                                                               cACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTccc                                 <  1:8933790/151‑1 (MQ=255)

                                                                                                               cACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTccc                                 >  2:1557644/1‑151 (MQ=255)

                                                                                                                aCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcg                                >  2:7807061/1‑151 (MQ=255)

                                                                                                                 ccGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                               <  1:2814512/151‑1 (MQ=255)

                                                                                                                 ccGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                               >  2:1958580/1‑151 (MQ=255)

                                                                                                                   ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggcg                             <  1:9743847/151‑1 (MQ=255)

                                                                                                                    gtctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggcgg                            >  1:7776410/4‑151 (MQ=255)

                                                                                                                     tctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCggcggc                           <  2:4926879/149‑1 (MQ=255)

                                                                                                                      ctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCa                          >  2:2934685/2‑151 (MQ=255)

                                                                                                                        cGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAtt                        <  2:142655/151‑1 (MQ=255)

                                                                                                                        cGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAtt                        >  1:2344499/1‑151 (MQ=255)

                                                                                                                           ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTccc                     <  2:9853302/151‑1 (MQ=255)

                                                                                                                             tGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAGTCCCGGCGGCATTCCCAg                   >  2:80609/1‑151 (MQ=255)

                                                                                                                              gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGc                  <  1:3644316/151‑1 (MQ=255)

                                                                                                                                ccttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcga                <  2:2260724/146‑1 (MQ=255)

                                                                                                                                   tcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgaccc             <  1:3771589/149‑1 (MQ=255)

                                                                                                                                    cgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccg            >  2:1268607/2‑151 (MQ=255)

                                                                                                                                     gTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccga           <  1:9187698/151‑1 (MQ=255)

                                                                                                                                           gccgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCt     <  2:10101118/151‑1 (MQ=255)

                                                                                                                                           gccgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCt     >  1:4560688/1‑151 (MQ=255)

                                                                                                                                           gccgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCt     >  2:2613274/1‑151 (MQ=255)

                                                                                                                                              gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAcc  <  1:1476807/151‑1 (MQ=255)

TCATCCCATGCAACACAGCCAAGTGTCCCCCAACGCGCGCCAGCAACTGGCCGAGACCGTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACC  >  NC_008463/3387736‑3388028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`