Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG98.7% 1510.1 / NA 468K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (256/204);  minor base T (6/0);  total (264/204)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428
                                                                                                                                                    |                                                                                                                                                   
cccgctgcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGCCCGCCGACCTCGCCGCGGGGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   <  2:1875773/145‑1 (MQ=255)
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   <  2:2789718/151‑1 (MQ=255)
  agcagcACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcgg                                                                                                                                                 >  2:6094957/1‑151 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAg                                                                                                                                          <  2:973586/151‑1 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAg                                                                                                                                          <  1:4297954/151‑1 (MQ=255)
           ggCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCa                                                                                                                                        >  2:1148437/1‑151 (MQ=255)
           ggCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATATACGTGGAGAAGGCGGCGGACAGCa                                                                                                                                        >  1:7043456/1‑151 (MQ=255)
              cAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCCTcc                                                                                                                                     >  2:9624189/1‑151 (MQ=255)
               aGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCa                                                                                                                                    <  2:7318108/151‑1 (MQ=255)
               aGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCa                                                                                                                                    <  1:4184497/151‑1 (MQ=255)
                   ttCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTg                                                                                                                                >  2:3916925/1‑151 (MQ=255)
                      gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGGGAAGGCGGCGGACCGCATCCAGCGGATc                                                                                                                             >  1:326685/1‑151 (MQ=255)
                        tgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGACGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTTCGTGGAGAAGGCGGCGGTCAGCATACAGCGGATCGt                                                                                                                           >  2:2733585/1‑151 (MQ=255)
                        tgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGCATACAGCTGATCGt                                                                                                                           >  1:474593/1‑151 (MQ=255)
                         gaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTc                                                                                                                          >  1:10436229/1‑151 (MQ=255)
                          accctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCGGATCGTCa                                                                                                                         >  2:5020622/1‑151 (MQ=255)
                           ccctgaccTGCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAc                                                                                                                        <  1:8124748/151‑1 (MQ=255)
                               gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGTGAAGGCGGCGGACAGCATCCAGCTGATCGGCACCCTc                                                                                                                    >  2:4700122/1‑151 (MQ=255)
                               gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCAGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTc                                                                                                                    >  1:8031272/1‑151 (MQ=255)
                                 ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAc                                                                                                                  <  1:8547721/151‑1 (MQ=255)
                                  cTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATAGTCACCCTCAcc                                                                                                                 >  1:9464095/1‑151 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                          <  2:9507009/151‑1 (MQ=255)
                                          aGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAg                                                                                                         <  2:6583853/151‑1 (MQ=255)
                                            ggCGTGAACGCCGACCTCGCCGCGGTGGTAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGGGGGTATCTTCGGGGAGACGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCGCCTCCcgcg                                                                                                       >  1:2483377/1‑151 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGcc                                                                                                    <  1:1193720/151‑1 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCGCCTCCAGCGGcc                                                                                                    >  2:7144765/1‑151 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACCGCATCCAGCTGATCGGCACCCTCACCTCCTCCAGCGGcc                                                                                                    >  2:8553330/1‑151 (MQ=255)
                                                  aaCGCCGACCTCGCCGCGGTGGAAGTGCAGACCCGCCTGCAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCt                                                                                                 <  2:10249774/151‑1 (MQ=255)
                                                  aaCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGTATCTACGTGGAGAATGCGGCGGTCAGCATCCAGCGGATCGTCACCCTCACCTCCTCCAGCGGCCGcg                                                                                                 >  1:10089003/1‑150 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGCGGCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacg                                                                                              >  2:4894694/1‑151 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCGCCAGCGGCCGCTacg                                                                                              >  2:10826147/1‑151 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTAGCCCCTGCCCGAGTCGGTGCGGCGCGAAGGTATCAAAGTGGAGAAGGCGGCGGACAGCAACCAGCAGATCGTCACCCTCACCACCGACAGCGGCCGCTaag                                                                                              >  2:3065297/1‑149 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgacg                                                                                           <  1:4077736/151‑1 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCAAACAGCAGATAGTCACCCACACCACCACCCGCGGCCGCTaccacg                                                                                           >  1:3784852/1‑151 (MQ=255)
                                                          ccTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGcc                                                                                         <  2:508940/151‑1 (MQ=255)
                                                          ccTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGcc                                                                                         <  2:3159930/151‑1 (MQ=255)
                                                            tCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACCGCATCCAGCTGATCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCAt                                                                                       >  1:9840069/1‑151 (MQ=255)
                                                             cgccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGCCGGTATCTACGTGGAGAAGGCGGCGGACACCATCCAGCTGATCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCATg                                                                                      >  1:5866877/1‑151 (MQ=255)
                                                             cgccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATg                                                                                      <  1:6748722/151‑1 (MQ=255)
                                                               ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGa                                                                                    <  1:8981647/151‑1 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACt                                                                                 <  1:9933733/151‑1 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGGCGGGATCTAAGTGGAGAAGGCGGCGGACAGCCTCCAGCGGATCGTCACCCGCACCACCTCCCGCGGCCGCGACGCCGCCATGGAACt                                                                                 >  1:1117011/1‑151 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTg                                                                                >  2:5670423/1‑151 (MQ=255)
                                                                    gTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGANCTgg                                                                               <  2:454988/151‑1 (MQ=255)
                                                                    gTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTgg                                                                               >  1:6858488/1‑151 (MQ=255)
                                                                     tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCAGACAGCTGATCGTCACCCTCCCCTCCTCCAGCGGCCGCTACGACGCCCTGGAACTggg                                                                              >  1:1422683/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGATGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGAGGACAGCATCCAGCTGATCGTCTCCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             <  1:10753700/151‑1 (MQ=255)
                                                                         aGTGCAGAACCGCCTGAAGATCGTCGAGTCGCCCCAGCCCCAGTCGGTGCGGCGCGACGGTATCAACGTGGAGAACGCGGCGGACAGCCTCCACCTGGACCTCACCCTCACCTCCTCCAGCCGCCGCTACGACGCCCTGGAACAGGGCcag                                                                          >  2:526657/1‑151 (MQ=255)
                                                                           tGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGAt                                                                        <  1:9227638/151‑1 (MQ=255)
                                                                            gCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATc                                                                       >  2:10558524/1‑151 (MQ=255)
                                                                            gCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATc                                                                       <  2:6153986/151‑1 (MQ=255)
                                                                              aGAACCGCCTGACGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGc                                                                     <  1:623408/151‑1 (MQ=255)
                                                                              aGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCCAGTCGGTGCGGCGCGACGGTATATACGTGGTGAAGGCGGCGGACAGCATACAGCAGATCGTCACCCACACCGCCTCCAGCGGCCGCTACCAAGCCAAGGAACAGGGCGAGATAGc                                                                     >  1:2833643/1‑151 (MQ=255)
                                                                                aaCCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCt                                                                   <  1:1866193/151‑1 (MQ=255)
                                                                                aaCCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTTCGGCGCGACGGGATCTACGTGGGTAAGGCGGCGGAAAGCATCCAGCTGATCGTCACCCTCACCGCCAACAGCGGCCGCTAAGACGCCCTGGAACTGGGCGAGATCGCCt                                                                   >  1:5983854/1‑151 (MQ=255)
                                                                                 aCCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTc                                                                  <  2:5702799/151‑1 (MQ=255)
                                                                                    gCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTc                                                               <  2:3728302/151‑1 (MQ=255)
                                                                                     ccTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTcc                                                              <  1:2938311/151‑1 (MQ=255)
                                                                                        gAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGAAGGTATATACGTGGAGAAGGCGGCGGACAGCAACCAGCTGATCGTCACCCTCACCTCCGCCCGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAAc                                                           >  2:4413652/1‑151 (MQ=255)
                                                                                         aaGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACg                                                          <  2:8808171/151‑1 (MQ=255)
                                                                                         aaGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACg                                                          <  2:5875186/151‑1 (MQ=255)
                                                                                                tcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCa                                                   >  1:639536/1‑151 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                  <  2:8430100/151‑1 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGAGCGCCTCGTCCAACGTGTTGctg                                                  >  2:2173190/1‑149 (MQ=255)
                                                                                                  gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                 >  2:1392759/1‑151 (MQ=255)
                                                                                                   cgTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                                <  2:1669744/150‑1 (MQ=255)
                                                                                                   aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                                >  1:2226557/1‑151 (MQ=255)
                                                                                                    gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                               <  1:351718/151‑1 (MQ=255)
                                                                                                     tCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGACCTGGGCGAGATCGCCTCGTCCACCGTGTTGCAGgcgc                                              >  1:556192/1‑151 (MQ=255)
                                                                                                       gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                            <  1:2704697/151‑1 (MQ=255)
                                                                                                       gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                            >  2:2551107/1‑151 (MQ=255)
                                                                                                       gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                            <  2:628268/151‑1 (MQ=255)
                                                                                                         gcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCCACGTGTTGCAGGCGCTgcg                                          >  2:7568272/1‑151 (MQ=255)
                                                                                                          ccTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgcgc                                         >  1:8695481/1‑151 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                      >  1:4462980/1‑151 (MQ=255)
                                                                                                              cccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCggg                                     <  2:3982033/151‑1 (MQ=255)
                                                                                                               ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGt                                    <  1:3118486/151‑1 (MQ=255)
                                                                                                                     cGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAggg                              >  1:842199/1‑151 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             >  1:5107796/1‑151 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGCCCGCTACGACGCCATGGAACAGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             >  2:6438704/1‑151 (MQ=255)
                                                                                                                         gcggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTg                          <  1:4634787/151‑1 (MQ=255)
                                                                                                                           ggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTggg                        >  2:5508361/1‑151 (MQ=255)
                                                                                                                           ggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTggg                        >  2:7794735/1‑151 (MQ=255)
                                                                                                                             cgcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                      >  2:5329551/1‑151 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCGACGACGCCATGGAACTGGGCGAGAACGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                    <  2:2078109/151‑1 (MQ=255)
                                                                                                                                gACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                   >  1:9706100/1‑151 (MQ=255)
                                                                                                                                 aCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                  >  2:6747694/1‑151 (MQ=255)
                                                                                                                                 aCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                  >  2:9128522/1‑151 (MQ=255)
                                                                                                                                  cGGTATCTACGTGGAGATGGCGGGGTACAGCATCCCGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGATACGACGCCTTGGAACTGGGCGAGATCGCCTCGTCAAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTc                 <  2:7964997/151‑1 (MQ=255)
                                                                                                                                  cGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTc                 >  2:10356371/1‑151 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               <  2:3509197/151‑3 (MQ=255)
                                                                                                                                      aTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCgaga             >  2:4337767/1‑151 (MQ=255)
                                                                                                                                      aTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCgaga             <  2:8172493/151‑1 (MQ=255)
                                                                                                                                      aTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCgaga             <  1:7891944/151‑1 (MQ=255)
                                                                                                                                       tCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAc            <  1:6607292/151‑1 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg         >  1:7416102/1‑151 (MQ=255)
                                                                                                                                           cGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgg        >  2:4793254/1‑151 (MQ=255)
                                                                                                                                              ggAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgc     >  1:10195681/1‑151 (MQ=255)
                                                                                                                                                agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc   >  2:6991808/1‑151 (MQ=255)
                                                                                                                                                agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc   >  2:929550/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑