Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,566,730 A→G pseudogene (1081/1275 nt) PA14_RS16215 → fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,566,7300AG99.8% 1943.6 / ‑4.4 505pseudogene (1081/1275 nt)PA14_RS16215fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (236/268);  minor base . (0/1);  total (236/269)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 505 total aligned reads displayed.

GAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCC  >  NC_008463/3566582‑3566876
                                                                                                                                                    |                                                                                                                                                   
gAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAg                                                                                                                                                  >  2:747755/1‑151 (MQ=255)
gAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAg                                                                                                                                                  >  1:222650/1‑151 (MQ=255)
   ccTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTGCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGATc                                                                                                                                               >  2:6159302/1‑151 (MQ=255)
     tGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCCGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCACCGAGGCGCGCCCCTCGAAGTTCgg                                                                                                                                             >  2:10447574/1‑151 (MQ=255)
     tGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCgg                                                                                                                                             <  1:10015845/151‑1 (MQ=255)
     tGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCgg                                                                                                                                             <  1:5125638/151‑1 (MQ=255)
      gggCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGc                                                                                                                                            >  2:8462441/1‑151 (MQ=255)
         cGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCg                                                                                                                                         >  2:7395126/1‑151 (MQ=255)
                ctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccg                                                                                                                                  >  1:6138527/1‑150 (MQ=255)
                ctgATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccg                                                                                                                                  <  2:577665/151‑2 (MQ=255)
                  gATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgag                                                                                                                                <  2:5141151/151‑4 (MQ=255)
                   aTCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgaga                                                                                                                               >  2:152873/1‑147 (MQ=255)
                    tCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCCTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgcgac                                                                                                                              >  2:8093976/1‑146 (MQ=255)
                    tCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGAccgagac                                                                                                                              <  1:10545558/151‑6 (MQ=255)
                     cGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCACGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGCGGCGCGCCCCTCGACGTTCGGCCCGATGAccgagtcc                                                                                                                             >  2:5901241/1‑145 (MQ=255)
                      gACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCt                                                                                                                            >  1:293335/1‑151 (MQ=255)
                        cATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCTAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTgg                                                                                                                          <  2:827790/151‑1 (MQ=255)
                        cATCCCGCTGCGGGGGTTCTCCTTCCTGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTgg                                                                                                                          <  1:10623177/151‑1 (MQ=255)
                          tCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTgggg                                                                                                                        <  2:6023233/151‑1 (MQ=255)
                             cGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCAt                                                                                                                     >  2:5714929/1‑151 (MQ=255)
                                tGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCAtgtg                                                                                                                  >  2:10032671/1‑151 (MQ=255)
                                    ggTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGag                                                                                                              <  2:1335213/151‑2 (MQ=255)
                                      tgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                            <  1:2051737/151‑1 (MQ=255)
                                      tgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCGGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                            <  2:10320236/151‑1 (MQ=255)
                                       gtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAAc                                                                                                           <  1:8546832/151‑1 (MQ=255)
                                        ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                          >  1:8966008/1‑151 (MQ=255)
                                        ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                          <  2:2175838/151‑1 (MQ=255)
                                         tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                         >  1:10413374/1‑151 (MQ=255)
                                         tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                         <  2:4246028/151‑1 (MQ=255)
                                         tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                         <  1:4451991/151‑1 (MQ=255)
                                         tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                         <  1:8205707/151‑1 (MQ=255)
                                          ctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTc                                                                                                        <  2:9589908/151‑1 (MQ=255)
                                            ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCt                                                                                                      <  2:5305866/151‑1 (MQ=255)
                                             cTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGACCTTCCTg                                                                                                     >  2:504470/1‑151 (MQ=255)
                                              ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                    >  2:9400049/1‑151 (MQ=255)
                                              ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                    <  1:10738858/151‑1 (MQ=255)
                                              ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                    >  1:6265196/1‑151 (MQ=255)
                                                 aTGCAGGACCTGCCCAGCCACGACTTCCACC‑CCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATcc                                                                                                 <  2:3906635/151‑1 (MQ=255)
                                                    cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                                              <  1:7463078/151‑1 (MQ=255)
                                                    cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                                              >  2:1213889/1‑151 (MQ=255)
                                                    cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTACTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                                              <  2:3279184/151‑1 (MQ=255)
                                                      ggACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgc                                                                                            >  1:4782317/1‑151 (MQ=255)
                                                         ccTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGac                                                                                         >  2:5558758/1‑151 (MQ=255)
                                                          cTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGacc                                                                                        <  2:5430508/151‑1 (MQ=255)
                                                            gCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                      >  2:10244370/1‑151 (MQ=255)
                                                            gCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                      >  1:1085120/1‑151 (MQ=255)
                                                             cccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccacc                                                                                     <  1:9389668/151‑1 (MQ=255)
                                                              ccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACct                                                                                    <  1:686626/151‑1 (MQ=255)
                                                                  ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGc                                                                                <  2:3461503/151‑1 (MQ=255)
                                                                   cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                               >  1:4646583/1‑151 (MQ=255)
                                                                     cGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgc                                                                             >  2:7734966/1‑151 (MQ=255)
                                                                        cTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                          <  2:7876596/151‑1 (MQ=255)
                                                                         ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                         >  1:5977015/1‑151 (MQ=255)
                                                                          tCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                        >  1:7211854/1‑151 (MQ=255)
                                                                           ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                                       >  1:8299955/1‑151 (MQ=255)
                                                                              accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTc                                                                     <  2:4452161/147‑1 (MQ=255)
                                                                              accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTc                                                                     >  2:6403998/5‑151 (MQ=255)
                                                                              accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTc                                                                     >  2:2722727/5‑151 (MQ=255)
                                                                               ccaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCg                                                                    <  1:10120108/148‑1 (MQ=255)
                                                                                caccGCAAGCCGGGGGTGGACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGATACCTGGGGCATGTGGGAGAACTTCCTGAACCTGCGCGACCACCTCTGCCGCGGCCAGTCgg                                                                   >  2:4584091/3‑151 (MQ=255)
                                                                                 accGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGa                                                                  >  2:8841106/2‑151 (MQ=255)
                                                                                    gCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAccc                                                               >  2:1828339/1‑151 (MQ=255)
                                                                                       aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                            <  1:4225141/151‑1 (MQ=255)
                                                                                       aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                            >  1:6505372/1‑151 (MQ=255)
                                                                                        gCCGGGGGTGAACTTCCGGCGCAACGCGCCGGAGCGCGCGGCCAACGCGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGCGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTc                                                           <  2:2544337/151‑1 (MQ=255)
                                                                                         ccGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCg                                                          <  1:3661584/151‑1 (MQ=255)
                                                                                             gggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                     <  1:9660167/151‑3 (MQ=255)
                                                                                                 gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAAtcgtc                                                  >  1:2606364/1‑151 (MQ=255)
                                                                                                   aCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGa                                                >  2:4696945/1‑151 (MQ=255)
                                                                                                   aCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGa                                                >  1:1584082/1‑151 (MQ=255)
                                                                                                   aCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGa                                                >  1:3746318/1‑151 (MQ=255)
                                                                                                    cTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGAc                                               <  2:8371655/151‑1 (MQ=255)
                                                                                                    cTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGAc                                               >  2:3083411/1‑151 (MQ=255)
                                                                                                        tGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGt                                           <  2:7120998/151‑1 (MQ=255)
                                                                                                           cgcCTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGGAcc                                        <  2:3694790/151‑1 (MQ=255)
                                                                                                           cgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTAcc                                        >  1:1383820/1‑151 (MQ=255)
                                                                                                            gcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcg                                       >  2:9669023/1‑151 (MQ=255)
                                                                                                               tCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCga                                    >  2:6098434/1‑151 (MQ=255)
                                                                                                                cgcgCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCgag                                   <  1:9127129/151‑1 (MQ=255)
                                                                                                                cgcgCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCgag                                   >  2:6610885/1‑151 (MQ=255)
                                                                                                                  cgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGaa                                 >  1:5744450/1‑151 (MQ=255)
                                                                                                                   gcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAAc                                >  2:10591352/1‑151 (MQ=255)
                                                                                                                    ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAAcc                               <  1:6632383/151‑1 (MQ=255)
                                                                                                                     cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCa                              <  1:548050/151‑1 (MQ=255)
                                                                                                                      ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCAt                             <  1:190854/151‑1 (MQ=255)
                                                                                                                       gAGCGCGCGGCCAACGAGGCGCGCCCCTCGACGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATg                            <  2:6305260/151‑1 (MQ=255)
                                                                                                                          cgcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGccc                         <  1:7627588/151‑1 (MQ=255)
                                                                                                                          cgcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGccc                         <  2:10764379/151‑1 (MQ=255)
                                                                                                                              cgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgct                     >  1:8829136/1‑151 (MQ=255)
                                                                                                                               ggCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                    >  1:3431988/1‑151 (MQ=255)
                                                                                                                               ggCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                    <  1:6665578/151‑1 (MQ=255)
                                                                                                                               ggCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                    <  1:244864/151‑1 (MQ=255)
                                                                                                                               ggCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctc                    <  1:10333211/151‑1 (MQ=255)
                                                                                                                                gCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctcg                   <  1:3176054/151‑1 (MQ=255)
                                                                                                                                   aacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgctc                <  2:6858705/151‑1 (MQ=255)
                                                                                                                                     cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCg              <  2:9769173/149‑1 (MQ=255)
                                                                                                                                        ggCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGgag           <  2:1994240/151‑1 (MQ=255)
                                                                                                                                              ccccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGAt     <  1:6104172/151‑1 (MQ=255)
                                                                                                                                               cccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGTCTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATg    <  1:783257/151‑1 (MQ=255)
                                                                                                                                                 cTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgcc  >  1:9260913/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGCCTGGGCGTGCTGCTGATCGACATCCCGCTGCGGGTGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCC  >  NC_008463/3566582‑3566876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑