Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,997,7860TC100.0% 1860.0 / NA 479K173E (AAG→GAG) PA14_RS22840DUF853 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (232/247);  total (232/247)

Only 100 of 479 total aligned reads displayed.

CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933
                                                                                                                                                    |                                                                                                                                                   
cgcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGc                                                                                                                                                   <  2:4573970/151‑1 (MQ=255)
cgcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGc                                                                                                                                                   >  1:3488704/1‑151 (MQ=255)
   ccccGCCCGTCCGGCTCGGGGCGGAGTATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGACCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGa                                                                                                                                                <  1:5739878/151‑1 (MQ=255)
   ccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGa                                                                                                                                                >  2:1274838/1‑151 (MQ=255)
     ccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAgg                                                                                                                                              <  1:684723/151‑1 (MQ=255)
     ccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAgg                                                                                                                                              <  1:502947/151‑1 (MQ=255)
      cgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGa                                                                                                                                             <  1:7308894/151‑1 (MQ=255)
       gcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                            >  1:315860/1‑151 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           <  1:9672795/151‑1 (MQ=255)
          cgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtctc                                                                                                                                         <  2:2252083/151‑1 (MQ=255)
          cgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtctc                                                                                                                                         <  2:6651440/151‑1 (MQ=255)
          cgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCGGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtctc                                                                                                                                         <  1:10305140/151‑1 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        >  1:2756718/1‑151 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        <  2:8501942/151‑1 (MQ=255)
             ccGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCgg                                                                                                                                      <  2:1352419/151‑1 (MQ=255)
                gCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAtt                                                                                                                                   >  2:5901466/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  2:3380957/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  2:3048017/1‑151 (MQ=255)
                  tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGt                                                                                                                                 >  1:10774954/1‑151 (MQ=255)
                  tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGt                                                                                                                                 >  2:10863134/1‑151 (MQ=255)
                    ggggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTcc                                                                                                                               >  2:4934257/1‑151 (MQ=255)
                     gggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCt                                                                                                                              >  2:1066001/1‑151 (MQ=255)
                     gggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCt                                                                                                                              <  2:6359785/151‑1 (MQ=255)
                      ggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtt                                                                                                                             >  2:2465890/1‑151 (MQ=255)
                      ggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtt                                                                                                                             >  2:6953900/1‑151 (MQ=255)
                       gCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTc                                                                                                                            <  2:9207865/151‑1 (MQ=255)
                       gCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTc                                                                                                                            <  1:8292411/151‑1 (MQ=255)
                                gTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGACGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCGGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTg                                                                                                                   <  1:4874492/151‑1 (MQ=255)
                                 tCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGa                                                                                                                  >  1:8651944/1‑151 (MQ=255)
                                  cTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGag                                                                                                                 >  1:1710056/1‑151 (MQ=255)
                                  cTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGag                                                                                                                 <  2:9584825/151‑1 (MQ=255)
                                   ttCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagc                                                                                                                >  2:8601134/1‑151 (MQ=255)
                                    tCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                               <  1:7911730/151‑1 (MQ=255)
                                     ccAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcag                                                                                                              >  1:9881259/1‑151 (MQ=255)
                                      cAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGTGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcagc                                                                                                             >  1:2518472/1‑151 (MQ=255)
                                       aGTTGCAGGGCAGGCTCGCCGTAGTAGGCCTCGGCGCCCTGCTGCTCCGGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGCGGATCTCCGGATTGTCCTTCAGGTGGTGGAGCAgcg                                                                                                            >  2:6222952/1‑151 (MQ=255)
                                        gTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcgc                                                                                                           >  2:5665328/1‑151 (MQ=255)
                                         ttGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcc                                                                                                          <  2:7570477/151‑1 (MQ=255)
                                          tGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcct                                                                                                         >  1:3963606/1‑151 (MQ=255)
                                          tGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcct                                                                                                         >  2:3822057/1‑151 (MQ=255)
                                             agggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttca                                                                                                      <  1:6337460/151‑1 (MQ=255)
                                             agggcaggCTCGCCGAAGAAGGCCTCGGCGCCCGGCTGCTCCCGAGTGGCCAGGCGGCGCAGCAGCGCCGGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttca                                                                                                      <  2:2729579/151‑1 (MQ=255)
                                                 caggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                  <  1:2217522/151‑1 (MQ=255)
                                                 caggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                  >  1:2314209/1‑151 (MQ=255)
                                                 caggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtc                                                                                                  >  2:6797996/1‑151 (MQ=255)
                                                  aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcc                                                                                                 <  1:3283545/151‑1 (MQ=255)
                                                   ggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcct                                                                                                <  1:755446/151‑1 (MQ=255)
                                                   ggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcct                                                                                                <  2:8349767/151‑1 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               <  1:1309219/151‑1 (MQ=255)
                                                     cTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttc                                                                                              >  1:10136664/1‑151 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         >  2:173720/1‑151 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         <  1:990624/151‑1 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         >  1:5146197/1‑151 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCAGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGCTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         >  2:360818/1‑151 (MQ=255)
                                                           gaagaaGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCg                                                                                        <  2:7261739/151‑1 (MQ=255)
                                                           gaagaaGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCATACAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCg                                                                                        >  2:523632/1‑151 (MQ=255)
                                                             agaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGag                                                                                      <  1:2635618/151‑1 (MQ=255)
                                                              gaagGCCTCGGCGCCCTGCTGCTCCAGCGGGGCCAGGCGGCGCAGCAGCGCCTGCCCGGCAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagc                                                                                     <  2:9834604/151‑1 (MQ=255)
                                                               aagGCCGCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCTGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCGGGTGGTTGAGCAGCGCCTTCAGGTCCGTCAGGTCGagca                                                                                    >  2:5148493/1‑151 (MQ=255)
                                                                 ggCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagc                                                                                  <  1:4056196/151‑1 (MQ=255)
                                                                 ggCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagc                                                                                  <  1:4747376/151‑1 (MQ=255)
                                                                  gCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagca                                                                                 >  2:9061438/1‑151 (MQ=255)
                                                                   ccTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcag                                                                                <  2:8048224/151‑1 (MQ=255)
                                                                       ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAgg                                                                            >  2:2571169/1‑151 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 <  2:2001883/151‑1 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 >  1:7094676/1‑151 (MQ=255)
                                                                                   ctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCg                                                                >  1:1886724/1‑151 (MQ=255)
                                                                                     ccAGAGCGGCCAGGCGGCGCAGCAGCGCCTGCCCGGCAGCTCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGCATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGTTCGAGCAGCAGCAGGCCTTCCCGGTCGGc                                                              <  2:8874967/151‑1 (MQ=255)
                                                                                             gCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGa                                                      <  1:4344369/151‑1 (MQ=255)
                                                                                             gCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGa                                                      <  1:5371788/151‑1 (MQ=255)
                                                                                              ccAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGaa                                                     <  1:1163569/151‑1 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAGTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   >  2:436307/1‑151 (MQ=255)
                                                                                                  gcggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcg                                                 <  1:6676972/151‑1 (MQ=255)
                                                                                                   cggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAGTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcgg                                                <  2:10087619/151‑1 (MQ=255)
                                                                                                    ggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcggc                                               <  2:1562450/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCTGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       >  2:3482983/1‑151 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCGTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       >  1:10553840/1‑151 (MQ=255)
                                                                                                              cgcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGcgcg                                     <  2:3753940/151‑1 (MQ=255)
                                                                                                                ccTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGc                                   <  1:363020/151‑1 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  <  1:9355560/151‑1 (MQ=255)
                                                                                                                  ggCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCACGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCct                                 <  1:3894028/150‑1 (MQ=255)
                                                                                                                        gcaGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATGGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGc                           <  2:9119547/149‑1 (MQ=255)
                                                                                                                         aaGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCt                          <  1:2384123/151‑1 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGt                    <  2:10257469/151‑1 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGGCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGACCAGCGCGGCCTGCTGGCTATCGGt                    <  2:4090533/151‑1 (MQ=255)
                                                                                                                                ggTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                   <  2:5425960/151‑1 (MQ=255)
                                                                                                                                ggTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTc                   >  2:4737969/1‑151 (MQ=255)
                                                                                                                                  tGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCaa                 <  1:8467109/151‑1 (MQ=255)
                                                                                                                                   gAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc                <  1:8906093/151‑1 (MQ=255)
                                                                                                                                     aCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTc              <  2:10023116/151‑1 (MQ=255)
                                                                                                                                     aCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTc              >  2:2503499/1‑151 (MQ=255)
                                                                                                                                       aGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCa            >  2:6095096/1‑151 (MQ=255)
                                                                                                                                       aGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCa            >  1:6891851/1‑151 (MQ=255)
                                                                                                                                          gcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagga         >  1:5904217/1‑151 (MQ=255)
                                                                                                                                          gcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGCCAACTCCagga         >  1:9557793/1‑151 (MQ=255)
                                                                                                                                           cccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggag        <  1:6204511/151‑1 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGCGCAGCGCCGTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       >  2:9533608/1‑151 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       <  1:5459808/151‑1 (MQ=255)
                                                                                                                                                tCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTgg   <  1:7837866/151‑1 (MQ=255)
                                                                                                                                                 ccTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGc  <  1:4227205/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑