Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,866,730 T→G A733A (GCA→GCC)  PA14_RS26890 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,866,7300TG99.8% 2227.6 / NA 582A733A (GCA→GCC) PA14_RS26890ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (346/234);  total (348/234)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 582 total aligned reads displayed.

CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877
                                                                                                                                                    |                                                                                                                                                   
 ggATGAGCGGATTCTTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcg                                                                                                                                                  <  1:6724869/151‑1 (MQ=255)
  gATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGTTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGgcgc                                                                                                                                                 <  1:6576117/151‑1 (MQ=255)
    tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                               <  1:1557952/151‑1 (MQ=255)
    tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCg                                                                                                                                               >  1:8698472/1‑151 (MQ=255)
       gCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcg                                                                                                                                            >  1:9739808/1‑151 (MQ=255)
         ggATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgcg                                                                                                                                          >  1:8373695/1‑151 (MQ=255)
         ggATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgcg                                                                                                                                          >  2:210911/1‑151 (MQ=255)
          gATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCgcgg                                                                                                                                         >  1:3710748/1‑151 (MQ=255)
                   cggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTa                                                                                                                                >  1:2326072/1‑151 (MQ=255)
                   cggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTa                                                                                                                                >  2:1351695/1‑151 (MQ=255)
                     gcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAcc                                                                                                                              <  1:8084430/151‑1 (MQ=255)
                        gATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAg                                                                                                                           >  1:7204032/1‑151 (MQ=255)
                        gATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAg                                                                                                                           <  1:2660995/151‑1 (MQ=255)
                         aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgg                                                                                                                          >  2:6339661/1‑151 (MQ=255)
                          tGTAAGCCGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGGTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGc                                                                                                                         <  1:6887772/151‑1 (MQ=255)
                            tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                       <  1:3120258/151‑1 (MQ=255)
                            tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                       <  1:1952380/151‑1 (MQ=255)
                            tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGt                                                                                                                       <  2:7772829/151‑1 (MQ=255)
                             aaGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTg                                                                                                                      >  2:3910008/1‑151 (MQ=255)
                             aaGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTg                                                                                                                      >  1:1685340/1‑151 (MQ=255)
                              aGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTgc                                                                                                                     >  2:7524934/1‑151 (MQ=255)
                                 cGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcc                                                                                                                  >  1:9294144/1‑151 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                 <  1:3523832/151‑1 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                 >  2:35884/1‑151 (MQ=255)
                                  gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                 >  1:4786503/1‑151 (MQ=255)
                                      ttGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAgg                                                                                                             >  2:469173/1‑151 (MQ=255)
                                      ttGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAgg                                                                                                             <  2:6217516/151‑1 (MQ=255)
                                       tGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGc                                                                                                            >  1:3303575/1‑151 (MQ=255)
                                         aaCGCGCGGATCTAAGATGTAAAAAGCTTTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGTCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGTCGTGCGCCCCAGGCTc                                                                                                          >  2:861431/1‑151 (MQ=255)
                                         aaCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTc                                                                                                          <  2:965296/151‑1 (MQ=255)
                                           cgcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgc                                                                                                        <  1:10305585/151‑1 (MQ=255)
                                              gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCggg                                                                                                     <  1:5217223/151‑1 (MQ=255)
                                              gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCggg                                                                                                     >  1:451099/1‑151 (MQ=255)
                                                ggATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTc                                                                                                   <  1:1243936/151‑1 (MQ=255)
                                                  aTCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGa                                                                                                 >  2:91565/1‑151 (MQ=255)
                                                  aTCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGa                                                                                                 >  2:2241179/1‑151 (MQ=255)
                                                       aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCg                                                                                            <  1:5649711/151‑1 (MQ=255)
                                                       aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCg                                                                                            >  2:4979205/1‑151 (MQ=255)
                                                       aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCg                                                                                            <  2:4287469/151‑1 (MQ=255)
                                                       aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCg                                                                                            >  1:4617652/1‑151 (MQ=255)
                                                       aGATGTAAAAAGCCCTAAGGAAGATGCTCGCCGCGCCGCTCACCCGGCCGCTGACACGGCCACGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCg                                                                                            <  2:1854551/151‑1 (MQ=255)
                                                        gATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCgg                                                                                           >  1:6104286/1‑151 (MQ=255)
                                                        gATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCgg                                                                                           >  2:8270831/1‑151 (MQ=255)
                                                         aTGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                          <  1:1757407/151‑1 (MQ=255)
                                                         aTGTAAAAAGCTGTAAGGAAGTTGGTCGCCGCGCCGCTCAACCGGCCGATGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                          <  2:5316097/151‑1 (MQ=255)
                                                            tAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgc                                                                                       >  2:1551236/1‑151 (MQ=255)
                                                            tAAAAAGCTGTAAGGAAGGTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgc                                                                                       >  1:10709494/1‑151 (MQ=255)
                                                                 aGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCa                                                                                  >  2:10018830/1‑151 (MQ=255)
                                                                   cTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAgg                                                                                >  2:5121591/1‑151 (MQ=255)
                                                                    tGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAggg                                                                               >  2:1799381/1‑151 (MQ=255)
                                                                    tGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAggg                                                                               >  2:10267697/1‑151 (MQ=255)
                                                                     ggaaGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGc                                                                              >  2:2463790/3‑151 (MQ=255)
                                                                      tAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGcc                                                                             >  2:8949362/1‑151 (MQ=255)
                                                                       aaGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCt                                                                            <  2:3678910/151‑1 (MQ=255)
                                                                          gAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcgc                                                                         <  1:395068/151‑1 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     >  1:9623838/1‑151 (MQ=255)
                                                                              ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTc                                                                     >  2:4444361/1‑151 (MQ=255)
                                                                                gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCa                                                                   <  1:4901238/151‑1 (MQ=255)
                                                                                gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCAACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCa                                                                   >  1:3390358/1‑151 (MQ=255)
                                                                                 ttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGTCTTCGACGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCTGGTCGACCCCCGCCGCGCCCAGGGCCTCGCGCTCCAg                                                                  >  2:5796759/1‑151 (MQ=255)
                                                                                   cgccgcgccgCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCt                                                                <  1:4191008/151‑1 (MQ=255)
                                                                                   cgccgcgccgCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCt                                                                <  2:7462214/151‑1 (MQ=255)
                                                                                    gccgcgccgcTCAACCGGCCGCTGGGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTc                                                               <  1:2126857/151‑1 (MQ=255)
                                                                                    gccgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTc                                                               >  2:3158113/1‑151 (MQ=255)
                                                                                     ccgcgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCg                                                              >  2:5587445/1‑151 (MQ=255)
                                                                                        cgccgcTCAACCGGCCGCTGTGTCGGCTTCTCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGAGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGccc                                                           >  1:753415/1‑151 (MQ=255)
                                                                                        cgccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGccc                                                           <  1:8469469/151‑1 (MQ=255)
                                                                                         gccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                          >  2:6956159/1‑151 (MQ=255)
                                                                                         gccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                          >  1:8410031/1‑151 (MQ=255)
                                                                                           cgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAgg                                                        <  1:4278890/151‑1 (MQ=255)
                                                                                           cgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAgg                                                        >  1:9490961/1‑151 (MQ=255)
                                                                                             cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGcc                                                      <  2:729081/151‑1 (MQ=255)
                                                                                               cAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCgg                                                    >  2:6777211/1‑151 (MQ=255)
                                                                                               cAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCgg                                                    >  2:7829553/1‑151 (MQ=255)
                                                                                                acccGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTAGCCAGGCGGGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCCGGGCCTCGCGCTCCAGCTCGCCCAGGCCGgc                                                   <  1:5312131/149‑1 (MQ=255)
                                                                                                   cGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACGCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGcc                                                <  1:2519281/151‑1 (MQ=255)
                                                                                                    ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCg                                               >  2:3095834/1‑151 (MQ=255)
                                                                                                      ccGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAg                                             >  1:10546810/1‑151 (MQ=255)
                                                                                                      ccGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAg                                             >  1:8999902/1‑151 (MQ=255)
                                                                                                       cGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgc                                            >  1:1419450/1‑151 (MQ=255)
                                                                                                          tGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTGCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcc                                         >  1:6377475/1‑151 (MQ=255)
                                                                                                           gAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcca                                        <  1:7290748/151‑1 (MQ=255)
                                                                                                                ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAgg                                   >  2:10609002/1‑151 (MQ=255)
                                                                                                                   ttCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                                >  2:10854915/1‑151 (MQ=255)
                                                                                                                    tCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCGACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCa                               <  2:8714886/151‑1 (MQ=255)
                                                                                                                      gCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATg                             >  1:9838632/1‑151 (MQ=255)
                                                                                                                       ccGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATgg                            >  2:9039179/1‑151 (MQ=255)
                                                                                                                         gAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAg                          >  1:5375733/1‑151 (MQ=255)
                                                                                                                               gCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAg                    >  1:7764137/1‑151 (MQ=255)
                                                                                                                                ccGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGc                   >  1:6640154/1‑151 (MQ=255)
                                                                                                                                 cggcgcggcgCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                  <  2:5885911/151‑1 (MQ=255)
                                                                                                                                    cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTcc               <  1:2888670/151‑1 (MQ=255)
                                                                                                                                    cgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTcc               <  2:9373914/151‑1 (MQ=255)
                                                                                                                                        gcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCCCCAGGCCCATGGAGTGGCAGCTTCCGGAc           <  2:5728325/151‑1 (MQ=255)
                                                                                                                                         cgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACa          >  1:10007390/1‑151 (MQ=255)
                                                                                                                                         cgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTGCCGGACa          >  2:3400432/1‑151 (MQ=255)
                                                                                                                                          gcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAg         <  1:9397328/151‑1 (MQ=255)
                                                                                                                                           cAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAgg        <  1:5901025/151‑1 (MQ=255)
                                                                                                                                            aGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc       <  2:2816949/151‑1 (MQ=255)
                                                                                                                                               gCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgat    >  2:6557870/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATGG  >  NC_008463/5866582‑5866877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑