Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,165 N→G intergenic (+26/‑35) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1650NG99.8% 1477.9 / NA 393intergenic (+26/‑35)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (191/201);  minor base T (1/0);  total (192/201)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

GTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCCTGA  >  NC_008463/1128017‑1128314
                                                                                                                                                    |                                                                                                                                                     
gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGcc                                                                                                                                                     <  1:548241/151‑1 (MQ=255)
          aGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGATCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                           <  1:5818393/151‑1 (MQ=255)
          aGTACCAGAACACCAACACCGCGAGCCTCACCTCCACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                           <  2:2846012/151‑1 (MQ=255)
           gTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACGTAGTCGCGCTCAGCTGCGGCCTTAACGTCGGTTGTACGCCGGCCCCCTGCGAACCGGGCCGGCGAGCAGGa                                                                                                                                          >  1:4558425/1‑151 (MQ=255)
              ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                       >  2:10257388/1‑151 (MQ=255)
              ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                       >  2:289118/1‑151 (MQ=255)
                aGAACACATACACCGCGCGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAAATTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTac                                                                                                                                     <  1:3633442/151‑2 (MQ=255)
                 gAACCCCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTCCACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                    <  1:5663368/151‑1 (MQ=255)
                 gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                    <  2:10103795/151‑1 (MQ=255)
                 gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                    <  1:7144649/151‑1 (MQ=255)
                 gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                    <  1:5113863/151‑1 (MQ=255)
                  aaCACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCCAGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACg                                                                                                                                   >  1:2023447/1‑151 (MQ=255)
                   acacCTACACCGCGAGCCTCTCATACACCAACTTCTTCGACGCCAAGTACACCACCGTGCACGATCGCGACGTCGTCGCGCTCAGCTTAGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGTCCGGAGAGCAGGACATAAccg                                                                                                                                  >  2:2030351/1‑149 (MQ=255)
                      ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCCGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                               >  2:8717607/1‑151 (MQ=255)
                        tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacg                                                                                                                             >  2:3556111/1‑151 (MQ=255)
                        tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacg                                                                                                                             <  1:3480749/151‑1 (MQ=255)
                         acacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAAACGGGCCGGCGAGCAGGACATAACGATgacga                                                                                                                            >  2:2767025/1‑151 (MQ=255)
                           acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                          <  2:9760803/151‑1 (MQ=255)
                             cgcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGGCTTAACGAGGACGCCGAg                                                                                                                        >  2:5471785/1‑151 (MQ=255)
                             cgcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGAATTCGTCGCGCTCAGCTTAGGCATGAACATCAGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                        >  2:84846/1‑151 (MQ=255)
                              gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGGGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                       <  1:7450851/151‑1 (MQ=255)
                               cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                                      <  2:4086341/151‑1 (MQ=255)
                               cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                                      <  2:6955703/151‑1 (MQ=255)
                                gAGCCTCTCCTACACCAACTTCTTCGACGGCATGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGa                                                                                                                     <  1:10724116/151‑1 (MQ=255)
                                gAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGa                                                                                                                     >  1:9489378/1‑151 (MQ=255)
                                 aGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                                    <  2:5757178/151‑1 (MQ=255)
                                 aGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                                    >  2:9519203/1‑151 (MQ=255)
                                  gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                                   <  2:4988569/151‑1 (MQ=255)
                                  gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                                   <  2:37607/151‑1 (MQ=255)
                                  gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                                   <  1:3796097/151‑1 (MQ=255)
                                    ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACATCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTAACGAGGACGACGAGCGAATGa                                                                                                                 >  2:3020174/1‑151 (MQ=255)
                                     actcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTGAACGAGGACGACGAGCGAATGaa                                                                                                                <  2:74291/150‑1 (MQ=255)
                                       tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaaaa                                                                                                              <  1:9703019/151‑1 (MQ=255)
                                       tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCCCGCCTTCGTCGCGCTCAGCTTCGGCATTAAAATCTGATGGACGCCGGCCCGGTGCGCACCGGGCCGGCGAGCAGGACTTAACTAGGACGACGAGCGAATGaaaa                                                                                                              >  2:9097338/1‑151 (MQ=255)
                                        ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                             <  2:1172387/151‑1 (MQ=255)
                                         cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                            >  2:10735724/1‑151 (MQ=255)
                                         cTACACCAACTGCTTCGACGGCAAGTACCCCACCGGGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                            <  1:10183620/151‑1 (MQ=255)
                                           acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaac                                                                                                          <  1:8511597/151‑1 (MQ=255)
                                           acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaac                                                                                                          <  1:2234627/151‑1 (MQ=255)
                                           acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaacaac                                                                                                          <  2:8346332/151‑1 (MQ=255)
                                            cacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATTAAAACAACg                                                                                                         >  1:7006722/1‑151 (MQ=255)
                                                 aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                                    >  2:6522647/1‑151 (MQ=255)
                                                 aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                                    >  2:4016577/1‑151 (MQ=255)
                                                 aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTACTGATGTACGCCGGCCCGGGGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGACAACAACGAAGAt                                                                                                    >  1:2495476/1‑151 (MQ=255)
                                                   ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttc                                                                                                  >  2:4434670/1‑151 (MQ=255)
                                                   ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttc                                                                                                  >  2:664472/1‑151 (MQ=255)
                                                       tcGACGGCAAGTACACCACCGTTGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCTTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATtcttct                                                                                              <  2:6361149/151‑1 (MQ=255)
                                                        cGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTg                                                                                             >  2:1593782/1‑151 (MQ=255)
                                                         gACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGTAAACAACGAAGATTCTTCTGc                                                                                            >  1:6181724/1‑151 (MQ=255)
                                                         gACGGCAAGCACACCACCGCGGCCGATCGCGCCGACGTCGCGCTCTGCGGCGGCATGAACTTCTGAGGTACGCCGGCCCGCTGCGAACCGGGCCGGCGAGCACGCCTTCCCGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGc                                                                                            <  2:8946991/151‑1 (MQ=255)
                                                           cGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                          <  2:1038187/151‑1 (MQ=255)
                                                           cGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                          <  2:1372781/151‑1 (MQ=255)
                                                              cAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                                       <  1:1927872/151‑1 (MQ=255)
                                                               aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCg                                                                                      <  2:10144019/151‑1 (MQ=255)
                                                                  tACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgtg                                                                                   <  2:965144/151‑1 (MQ=255)
                                                                   acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgtgt                                                                                  <  1:519211/151‑1 (MQ=255)
                                                                      ccaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACt                                                                               <  2:9327254/151‑1 (MQ=255)
                                                                       caccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                              >  2:2635243/1‑151 (MQ=255)
                                                                       caccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                              <  1:6352718/151‑1 (MQ=255)
                                                                          cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGcc                                                                           >  2:4155803/1‑151 (MQ=255)
                                                                            tGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCt                                                                         <  2:608849/151‑1 (MQ=255)
                                                                            tGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCt                                                                         >  2:339010/1‑151 (MQ=255)
                                                                              gACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGa                                                                       >  1:1757758/1‑151 (MQ=255)
                                                                               aCGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAg                                                                      <  1:10309316/151‑1 (MQ=255)
                                                                                  aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCct                                                                   <  1:716443/151‑1 (MQ=255)
                                                                                  aTCGCGACTGCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCct                                                                   <  2:2083406/151‑1 (MQ=255)
                                                                                     gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                                >  2:4674858/1‑151 (MQ=255)
                                                                                     gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                                <  2:10606796/151‑1 (MQ=255)
                                                                                       gACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                              >  1:10544995/1‑151 (MQ=255)
                                                                                               cgcgCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAg                                                      >  2:9719611/1‑151 (MQ=255)
                                                                                                gcgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAgg                                                     >  1:9615431/1‑151 (MQ=255)
                                                                                                gcgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAgg                                                     <  1:8218102/151‑1 (MQ=255)
                                                                                                    tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                                 >  1:6450653/1‑151 (MQ=255)
                                                                                                    tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                                 >  2:7208234/1‑151 (MQ=255)
                                                                                                     cAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATg                                                <  1:4322699/151‑1 (MQ=255)
                                                                                                       gCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                              >  1:3168350/1‑151 (MQ=255)
                                                                                                       gCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                              <  1:5383887/151‑1 (MQ=255)
                                                                                                        cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                             >  1:8082448/1‑151 (MQ=255)
                                                                                                          tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGACGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcggc                                           <  1:1700618/151‑1 (MQ=255)
                                                                                                           cGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGgcggcg                                          >  1:8783066/1‑151 (MQ=255)
                                                                                                              cATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                                       >  2:3179281/1‑151 (MQ=255)
                                                                                                              cATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                                       <  1:7923412/151‑1 (MQ=255)
                                                                                                                tGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                                     >  2:4879996/1‑151 (MQ=255)
                                                                                                                  aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                                   <  2:811242/151‑1 (MQ=255)
                                                                                                                  aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                                   <  1:1325571/151‑1 (MQ=255)
                                                                                                                   aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                                  >  1:9876579/1‑151 (MQ=255)
                                                                                                                     ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                                <  1:1562216/151‑1 (MQ=255)
                                                                                                                     ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGGATccgccg                                <  1:10001452/151‑1 (MQ=255)
                                                                                                                            gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc                         <  2:530107/151‑1 (MQ=255)
                                                                                                                             tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg                        <  2:7867168/151‑1 (MQ=255)
                                                                                                                               cGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcggc                      >  1:3420174/1‑151 (MQ=255)
                                                                                                                                   ggCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg                  >  2:2878821/1‑151 (MQ=255)
                                                                                                                                      ccGGTGCGAACCGGGCCGGCGAGCAGGACTTACCGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc               <  2:5859916/151‑1 (MQ=255)
                                                                                                                                        ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg             >  2:2257341/1‑151 (MQ=255)
                                                                                                                                        ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGGGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg             >  2:8462835/1‑151 (MQ=255)
                                                                                                                                         gTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTCTCCGCCGACGAGGCGGCCAAGCTCGGc            >  2:10285574/1‑151 (MQ=255)
                                                                                                                                            cgaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCAcc         >  1:4046195/3‑151 (MQ=255)
                                                                                                                                              aaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAg       >  1:4939237/1‑151 (MQ=255)
                                                                                                                                                 cgggCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCCt    >  2:1806000/2‑151 (MQ=255)
                                                                                                                                                   ggCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCCTGa  <  1:9213648/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGCCTGA  >  NC_008463/1128017‑1128314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑