Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,356,5480GC99.8% 2230.9 / NA 584R69G (CGT→GGT) PA14_RS06375MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (214/369);  minor base A (1/0);  total (215/369)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 584 total aligned reads displayed.

ATGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCCG  >  NC_008463/1356400‑1356695
                                                                                                                                                    |                                                                                                                                                   
  gCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAg                                                                                                                                                 <  1:6050642/151‑1 (MQ=255)
  gCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAg                                                                                                                                                 <  1:8054276/151‑1 (MQ=255)
  gCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAg                                                                                                                                                 >  2:2640701/1‑151 (MQ=255)
     gTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                              >  1:742571/1‑151 (MQ=255)
     gTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                              >  1:3534180/1‑151 (MQ=255)
          aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                         <  1:4153276/151‑1 (MQ=255)
          aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                         >  1:3275137/1‑151 (MQ=255)
            ccGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAgg                                                                                                                                       <  2:5079391/151‑1 (MQ=255)
             cGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgc                                                                                                                                      <  1:8641298/151‑1 (MQ=255)
             cGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgc                                                                                                                                      <  1:9934905/151‑1 (MQ=255)
                ggaggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCt                                                                                                                                   <  2:1074882/151‑1 (MQ=255)
                ggaggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCt                                                                                                                                   >  1:2997149/1‑151 (MQ=255)
                   ggaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAg                                                                                                                                <  1:7663015/151‑1 (MQ=255)
                      cGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCAt                                                                                                                             >  2:8535060/1‑151 (MQ=255)
                        ccGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCt                                                                                                                           <  2:10624435/151‑1 (MQ=255)
                          gATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                         <  2:6697799/151‑1 (MQ=255)
                           aTCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcg                                                                                                                        >  1:5181378/1‑151 (MQ=255)
                               gggTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGt                                                                                                                    <  2:7311098/151‑1 (MQ=255)
                                      ggATACCCCCATCAGCAGCCGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                             <  2:6902621/151‑1 (MQ=255)
                                      ggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                             <  1:9477321/151‑1 (MQ=255)
                                        aTACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGt                                                                                                           >  1:9809287/1‑151 (MQ=255)
                                         tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTc                                                                                                          <  2:7645418/151‑1 (MQ=255)
                                         tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTc                                                                                                          >  1:7830400/1‑151 (MQ=255)
                                            ccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGc                                                                                                       <  2:4862413/151‑1 (MQ=255)
                                            ccccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGc                                                                                                       >  2:1593040/1‑151 (MQ=255)
                                             cccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                      <  1:67302/151‑1 (MQ=255)
                                                     cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                              <  2:4041219/151‑1 (MQ=255)
                                                     cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                              <  1:10053393/151‑1 (MQ=255)
                                                     cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                              <  1:6602030/151‑1 (MQ=255)
                                                     cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                              <  1:5403699/151‑1 (MQ=255)
                                                      agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATa                                                                                             >  2:8804105/1‑151 (MQ=255)
                                                       gcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAc                                                                                            <  1:1352266/151‑1 (MQ=255)
                                                        cagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAcc                                                                                           <  2:10141069/151‑1 (MQ=255)
                                                         agcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGACTACCg                                                                                          >  2:1711194/1‑151 (MQ=255)
                                                            gAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaaga                                                                                                <  1:9164130/142‑1 (MQ=255)
                                                            gAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAc                                                                                       >  1:10858093/1‑151 (MQ=255)
                                                             aGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGAcc                                                                                      <  2:3926128/151‑1 (MQ=255)
                                                              ggCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCg                                                                                     >  2:2795555/1‑151 (MQ=255)
                                                                cGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGAt                                                                                   <  2:3605260/151‑1 (MQ=255)
                                                                cGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGAt                                                                                   <  1:3670303/151‑1 (MQ=255)
                                                                    cAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                               >  1:2395224/1‑151 (MQ=255)
                                                                     aGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCa                                                                              >  1:8290604/1‑151 (MQ=255)
                                                                       ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                            <  2:4347201/151‑1 (MQ=255)
                                                                       ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                            <  2:9434431/151‑1 (MQ=255)
                                                                       ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAgg                                                                            <  1:2524828/151‑1 (MQ=255)
                                                                         ttGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggc                                                                          <  1:7292847/151‑1 (MQ=255)
                                                                               tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcggc                                                                    >  1:6857951/1‑151 (MQ=255)
                                                                                gcgcCCGCTGCTGTCGCCGACGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGCTACCGAAGGTGAGGAAGGCGCTGAGCCTCAGCGCGGTGCCGGATGTCTGCGGGAAGAATCCCGGACCGATCACCAGGGCGGCGGCa                                                                   <  2:7199849/151‑1 (MQ=255)
                                                                                gcgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCa                                                                   <  1:4418582/151‑1 (MQ=255)
                                                                                 cgctctaTGCTGTCGCCGAAGTGGCCGTACAGCGCCGAGCGCAGCGGGCGAGCGAGGTTGGCGATACCGATGGTGAGGAATGCGCTGAGCATCTGCGCGGTGCCTGTTGTCTGCGGGATGAATTCCGGACCTTTAACCAGTGCTGCGGCAg                                                                  <  2:9031431/145‑1 (MQ=255)
                                                                                  gcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                 <  1:4702096/151‑1 (MQ=255)
                                                                                  gcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                 <  1:3764362/151‑1 (MQ=255)
                                                                                  gcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                 <  2:9275467/151‑1 (MQ=255)
                                                                                   cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGACGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                                >  1:6316358/1‑151 (MQ=255)
                                                                                     cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                              >  2:10215624/1‑151 (MQ=255)
                                                                                     cGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGc                                                              <  2:4429616/151‑1 (MQ=255)
                                                                                       aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                            <  1:7937720/151‑1 (MQ=255)
                                                                                       aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                            <  1:1643808/151‑1 (MQ=255)
                                                                                        tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                           >  2:1399874/1‑151 (MQ=255)
                                                                                        tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                           <  2:8720013/151‑1 (MQ=255)
                                                                                         gCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAg                                                          <  2:5636284/151‑1 (MQ=255)
                                                                                          cGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGa                                                         <  1:5854382/151‑1 (MQ=255)
                                                                                            gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACg                                                       <  1:1250110/151‑1 (MQ=255)
                                                                                               gCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAg                                                    >  1:3934380/1‑151 (MQ=255)
                                                                                                ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGa                                                   <  2:10316609/151‑1 (MQ=255)
                                                                                                ccGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGa                                                   <  1:9225229/151‑1 (MQ=255)
                                                                                                   aaGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAAt                                                >  1:1808004/1‑151 (MQ=255)
                                                                                                      tGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGt                                             >  1:1983109/1‑151 (MQ=255)
                                                                                                       ggCCGAACAGCGCCGAGCCCAGCGGGCGTGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTa                                            <  2:534785/151‑1 (MQ=255)
                                                                                                       ggCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTa                                            <  1:8880571/151‑1 (MQ=255)
                                                                                                         ccGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGTCATAGACGTAGAAATCGTAGa                                          <  2:7757504/151‑1 (MQ=255)
                                                                                                         ccGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGa                                          <  2:3699041/151‑1 (MQ=255)
                                                                                                          cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGaa                                         <  1:10438793/151‑1 (MQ=255)
                                                                                                          cGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGaa                                         <  2:2078570/151‑1 (MQ=255)
                                                                                                             aCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTc                                      <  1:7877412/151‑1 (MQ=255)
                                                                                                              cAGCGCCGAGCCCAGCGGGCGAGCGTGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCg                                     >  1:2924023/1‑151 (MQ=255)
                                                                                                              cAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCg                                     <  2:7992160/151‑1 (MQ=255)
                                                                                                               aGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGa                                    >  1:2202613/1‑151 (MQ=255)
                                                                                                               aGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGa                                    >  2:1879495/1‑151 (MQ=255)
                                                                                                                  gcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                 >  1:9385772/1‑151 (MQ=255)
                                                                                                                  gcCGAGCCCAGCGGGCGAGCGAGGAAGGCGAGACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                 <  2:6068269/151‑1 (MQ=255)
                                                                                                                   ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAATGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTATGCGGGAAGAATACCGGAACGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGc                                <  2:3331099/151‑1 (MQ=255)
                                                                                                                     gAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCgg                              >  1:2798875/1‑151 (MQ=255)
                                                                                                                      aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGt                             <  1:5542995/151‑1 (MQ=255)
                                                                                                                      aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGt                             <  2:5836549/151‑1 (MQ=255)
                                                                                                                      aGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGt                             <  2:747444/151‑1 (MQ=255)
                                                                                                                        cccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATTTCTGCGGGTAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGc                           <  2:6227071/151‑1 (MQ=255)
                                                                                                                          cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCg                         <  2:4182894/151‑1 (MQ=255)
                                                                                                                            gCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGAt                       <  2:9618462/151‑1 (MQ=255)
                                                                                                                              gggCGAGCGAGGAAGGCGATACCGAAGGTGAGGACGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGACATCGTAGAATTCGATGGCGGTGCCGATGa                     <  2:466103/151‑1 (MQ=255)
                                                                                                                               ggCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGaa                    <  2:2447680/151‑1 (MQ=255)
                                                                                                                               ggCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGaa                    <  2:5441309/151‑1 (MQ=255)
                                                                                                                                 cgagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGNTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                  >  1:5689986/1‑151 (MQ=255)
                                                                                                                                   agcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTg                <  1:9625938/151‑1 (MQ=255)
                                                                                                                                   agcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTg                >  2:8183525/1‑151 (MQ=255)
                                                                                                                                    gcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTgg               <  2:9706359/151‑1 (MQ=255)
                                                                                                                                     cgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGc              <  2:2264385/151‑1 (MQ=255)
                                                                                                                                           aGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGc        <  2:1098802/151‑1 (MQ=255)
                                                                                                                                            ggCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCg       <  2:310455/151‑1 (MQ=255)
                                                                                                                                             gCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGa      >  2:5186043/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCCG  >  NC_008463/1356400‑1356695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑