Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,707,727 N→G ?75P (CNT→CCT)  PA14_RS12740 ← DUF2285 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,707,7270NG100.0% 1404.7 / NA 368?75P (CNT→CCT) PA14_RS12740DUF2285 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base G (187/181);  total (187/181)

Only 100 of 371 total aligned reads displayed.

GGCAGCAGCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCANGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGCCAGC  >  NC_008463/2707578‑2707876
                                                                                                                                                      |                                                                                                                                                     
  cagcagCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGa                                                                                                                                                    >  1:6373272/1‑151 (MQ=255)
    gcagcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAAc                                                                                                                                                  <  1:2683341/151‑1 (MQ=255)
     cagcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAAcc                                                                                                                                                 <  1:5789597/151‑1 (MQ=255)
      agcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCa                                                                                                                                                <  2:9350942/151‑1 (MQ=255)
      agcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCa                                                                                                                                                <  2:3808702/151‑1 (MQ=255)
         cGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGc                                                                                                                                             <  1:10530323/151‑1 (MQ=255)
          gggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGcc                                                                                                                                            >  1:1297880/1‑151 (MQ=255)
          gggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGcc                                                                                                                                            <  2:6163947/151‑1 (MQ=255)
           ggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCg                                                                                                                                           <  2:6861614/151‑1 (MQ=255)
              cAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGAt                                                                                                                                        <  2:4733413/151‑1 (MQ=255)
               aaTGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGACCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATCGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATg                                                                                                                                       >  2:6385852/1‑151 (MQ=255)
               aaTGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATCGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCCGGCCGGATg                                                                                                                                       >  2:15468/1‑151 (MQ=255)
                  gCGACACCATCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATgcgc                                                                                                                                    <  2:2819623/151‑1 (MQ=255)
                  gCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATgcgc                                                                                                                                    <  1:1635366/151‑1 (MQ=255)
                     acacCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGACCCAGGCCGGATGCGCGTc                                                                                                                                 >  1:8231830/1‑151 (MQ=255)
                     acacCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTc                                                                                                                                 <  1:4971372/151‑1 (MQ=255)
                      caccagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcg                                                                                                                                <  1:9786086/151‑1 (MQ=255)
                      caccagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcg                                                                                                                                >  1:9892532/1‑151 (MQ=255)
                      caccagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcg                                                                                                                                >  2:9876647/1‑151 (MQ=255)
                       accagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgc                                                                                                                               >  1:7538922/1‑151 (MQ=255)
                        ccagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcg                                                                                                                              <  2:6762823/151‑1 (MQ=255)
                        ccagcaccagGCGCGTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcg                                                                                                                              <  2:7080630/151‑1 (MQ=255)
                         cagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGTTAGAGCTGCACCACGGC‑TTCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcgc                                                                                                                             <  2:1804490/151‑1 (MQ=255)
                         cagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcgc                                                                                                                             <  2:7230126/151‑1 (MQ=255)
                          agcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcgcg                                                                                                                            >  1:3924368/1‑151 (MQ=255)
                          agcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGCGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgcgcg                                                                                                                            <  1:2526773/151‑1 (MQ=255)
                           gcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCgcgcgc                                                                                                                           >  1:7679388/1‑151 (MQ=255)
                             accagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAt                                                                                                                         >  1:4289986/1‑151 (MQ=255)
                               cagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATcc                                                                                                                       >  2:3637542/1‑151 (MQ=255)
                               cagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATcc                                                                                                                       <  1:9413832/151‑1 (MQ=255)
                                 ggCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCTGGAACGCGCCAGACCTCGACGGCATGGGCTTCGGGTGGCGGGGCGGCGCCGGGATAGAGCTGCACCACGGC‑AGCGGGGTCAGGGAACCAGGCCGGATGCGCGTGGCGCGCATCCaa                                                                                                                     <  2:8603209/151‑1 (MQ=255)
                                 ggCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCaa                                                                                                                     <  1:8870852/151‑1 (MQ=255)
                                  gcgcTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAg                                                                                                                    <  2:3194823/151‑1 (MQ=255)
                                   cgcTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAgg                                                                                                                   <  1:5292041/151‑1 (MQ=255)
                                      ttGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTg                                                                                                                >  1:2271330/1‑151 (MQ=255)
                                       tGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTgg                                                                                                               <  1:2845153/151‑1 (MQ=255)
                                         ccGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGt                                                                                                             <  2:7984326/151‑1 (MQ=255)
                                          cgtcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTc                                                                                                            >  2:10165377/1‑151 (MQ=255)
                                           gtcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGGGGGTCGGCGTCGGGATAGAGCTGCACCACGGA‑TTCGTGGTCTGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCt                                                                                                           <  1:10036342/151‑1 (MQ=255)
                                              gtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTcc                                                                                                        <  1:3145514/151‑1 (MQ=255)
                                              gtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTcc                                                                                                        <  1:4747794/151‑1 (MQ=255)
                                               tGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCca                                                                                                       >  1:3380119/1‑151 (MQ=255)
                                               tGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCca                                                                                                       >  2:1688019/1‑151 (MQ=255)
                                                ggATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcag                                                                                                      >  1:422359/1‑151 (MQ=255)
                                                   tCAGTTGCTTGCGGCCGGTAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGTATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                   <  1:3054101/151‑1 (MQ=255)
                                                   tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                   >  1:3705356/1‑151 (MQ=255)
                                                   tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                   >  1:3700458/1‑151 (MQ=255)
                                                   tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                   <  2:7660308/151‑1 (MQ=255)
                                                   tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                   <  2:8860229/151‑1 (MQ=255)
                                                     aGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggc                                                                                                 >  2:43034/1‑151 (MQ=255)
                                                     aGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAACCAAGGCTGGGTCTTCCAgcaggc                                                                                                 <  1:6574684/151‑1 (MQ=255)
                                                       gtgcttgcGGCCGGGAACGCGCCAGAACTCGACGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAACCAAGGCTGGGTCTTCCAgcaggcgc                                                                                               <  1:5403211/150‑1 (MQ=255)
                                                        tgcttgcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCGGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGAGGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgca                                                                                              <  1:4372362/151‑1 (MQ=255)
                                                         gcttgcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgcag                                                                                             <  2:623544/151‑1 (MQ=255)
                                                               ggCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCa                                                                                       >  1:9252266/1‑151 (MQ=255)
                                                                 ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAg                                                                                     <  2:7939395/151‑1 (MQ=255)
                                                                 ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAg                                                                                     >  1:10393115/1‑151 (MQ=255)
                                                                 ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAg                                                                                     <  2:3391461/151‑1 (MQ=255)
                                                                   gggAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGcc                                                                                   >  2:871386/1‑151 (MQ=255)
                                                                   gggAACGCCCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGcc                                                                                   <  1:3796028/151‑1 (MQ=255)
                                                                    gtaaCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCt                                                                                  <  2:2119364/149‑1 (MQ=255)
                                                                    ggAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCt                                                                                  >  1:6876004/1‑151 (MQ=255)
                                                                    ggAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCt                                                                                  <  2:4558499/151‑1 (MQ=255)
                                                                    ggAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCt                                                                                  <  2:10010126/151‑1 (MQ=255)
                                                                       aCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGCTAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcg                                                                               >  2:6441226/1‑151 (MQ=255)
                                                                        cgcgCCAGAACTCGAAGGCCTGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcgc                                                                              <  1:6982804/151‑1 (MQ=255)
                                                                        cgcgCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcgc                                                                              <  1:6699580/151‑1 (MQ=255)
                                                                        cgcgCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcgc                                                                              >  1:2083453/1‑151 (MQ=255)
                                                                              aGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTc                                                                        >  1:5572581/1‑151 (MQ=255)
                                                                                  cTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGc                                                                    >  1:1879038/1‑151 (MQ=255)
                                                                                  cTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGc                                                                    >  1:7310520/1‑151 (MQ=255)
                                                                                        ggCTTGGGTTTGGGGGGGGGGGTGGGGGTGGGTTTAGTGCTCCACCAGGGC‑ATGGTGTTCTGTTAACCAGGCCGTTTGGGCGTCGCGCGAATCCATGGATGGTTTTTCATGCTGGGGCTGGCTCCAAGCCTGCGCCGCGTCCGGCCAgcgg                                                              <  2:5278146/151‑1 (MQ=255)
                                                                                        ggCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAgcgg                                                              >  2:1258036/1‑151 (MQ=255)
                                                                                        ggCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAgcgg                                                              >  1:9026783/1‑151 (MQ=255)
                                                                                           aTGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGa                                                           >  2:9162326/1‑151 (MQ=255)
                                                                                            tGGGTTGCGGGTGGCGGGTCGGCGTCGGGAGAGAGCTGCACCACGGC‑AGGGTGTTCTGTGACCCAGGCGGGATGCGCGACGCGCGAACCAACGGCGGGGTCTTCCAGCCCGCGCAGGGACCAAGCCTGCGCCGCGTCCGGCCAGCGGCGAc                                                          <  2:7704062/151‑1 (MQ=255)
                                                                                                cTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGgcg                                                      >  2:4612024/1‑151 (MQ=255)
                                                                                                     gggggggggTCGGCGTCGGGATAGAGCGGCACCCCGGA‑ATCGTGGTCCGGGACCCTGGCCGTATGCGCGCCGCGCGCATCCACGGCTGGGTCTTCCAGCTGGCGCAGGCTCCACCCCGGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGcc                                                 <  2:6927777/145‑1 (MQ=255)
                                                                                                     ggTGGCGGGTCGGCGTCGGTTTAGCGCGGCACC‑CGGCAATCTGGTTCCGGGAACCTGGCCGTTTGCGCGTCGCGCGAATCCATGGCTGGGTATTCCAGCTGGCGCAGGCTCCACGCCTGGGCCGCGTCCGGCCAGCGGCGACGGCGCAGcc                                                 <  2:5694558/151‑1 (MQ=255)
                                                                                                       tGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCTAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCATGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAg                                               >  1:6738924/1‑151 (MQ=255)
                                                                                                       tGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAg                                               >  1:6304561/1‑151 (MQ=255)
                                                                                                         gggggCCGGCGTCGGCACACCGCGCCACCCCGGA‑TTGGTGGTCCGGAACCCCGGCCGGCGGCGCTTCGCGCGCAACCAAGGCGGGGTATTCCAGCAGCCGCTGGCTCCACGCCGGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTc                                             <  2:9472507/149‑1 (MQ=255)
                                                                                                           gggTGGGCTGCGGGACCGTGCTCCACCCCGGC‑ACGGTGGACGGGCAACCCGGCGGGATGCCGGCCCCGGCCACCCAAGGGGGGGTCTCCCCGCTGGGGCAGGCCCCCAGCCGGCCCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTcgc                                           <  2:5587894/151‑1 (MQ=255)
                                                                                                             gTCGGCTGCGGTATAGAGCGGCACCCCGGC‑ATCGTGTTCCGGGACCCCGGCGGGTGGCGCGGCGCGCCCACCCAAGGCTGGGTCGTCCACCAGGCGATGCCACCACCCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCgcgg                                         <  2:5799344/151‑1 (MQ=255)
                                                                                                              tCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCgcggc                                        >  2:2637486/1‑151 (MQ=255)
                                                                                                              tCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCgcggc                                        >  1:2341622/1‑151 (MQ=255)
                                                                                                                ggCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGcggcgg                                      >  1:7935347/1‑151 (MQ=255)
                                                                                                                  cGTCGGTATAGAGCCGCACCACGGC‑AACGGGGTCGGGGACCCCGGCCGGTGGCGCGCCGCGCGCAACCACGGGGGGGTCTCCCAGCAGGCGCCGGCGCCACCCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTa                                    <  2:7521204/151‑1 (MQ=255)
                                                                                                                     cGGGATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATc                                 >  1:506922/1‑151 (MQ=255)
                                                                                                                      gggATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCg                                >  2:5183803/1‑151 (MQ=255)
                                                                                                                        gATAGAGCTGCACCACGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCggg                              >  1:6847837/1‑151 (MQ=255)
                                                                                                                                    ccacGGC‑ATAGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAg                  >  1:2814009/1‑151 (MQ=255)
                                                                                                                                     cacGGC‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGGCGCGGCGGTAATCGGGATTCCTGCGCAgg                 >  2:1623423/1‑151 (MQ=255)
                                                                                                                                          c‑ATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCGGGCGCCGGCTCCAAGCCGGCGCCGCGTCCGGCCCGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGCTTCCTGCGCAGGTAGTc            >  2:1994086/1‑151 (MQ=255)
                                                                                                                                           ctccGTGGTCTGGGACCCCGGCCGGATGCGCGTCGCGCGAATCCATGGCTGGGTATTCCAGCAGGCGCTGGCTCCACGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTc            <  2:2431528/148‑1 (MQ=255)
                                                                                                                                             tCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTccc          >  2:8246704/1‑151 (MQ=255)
                                                                                                                                               gTGGTCAGGGACCCCGGCGGTATGCGCGTCGCGCGCAGCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCGTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCaa        <  2:5930724/151‑1 (MQ=255)
                                                                                                                                               gTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCaa        <  2:9602134/151‑1 (MQ=255)
                                                                                                                                                  gTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTATTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGcc     <  2:10695704/151‑1 (MQ=255)
                                                                                                                                                     aGGGAACCAGGCCGGAAGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTACGCGGGCTTCCTGCGCAGGTATTCCCAAGCCCgc  >  1:1494293/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGCAGCAGCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGC‑ATCGTGGTCANGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGCCAGC  >  NC_008463/2707578‑2707876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑