BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,730,681	2 bp→CG	intergenic (+19/+95)	PA14_RS12860 → / ← ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,730,681	0	G	C	100.0%	1322.6 / NA	343	intergenic (+19/+96)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (132/211); total (132/211)
*	NC_008463	2,730,682	0	C	G	99.7%	1300.8 / NA	343	intergenic (+20/+95)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (132/210); minor base T (0/1); total (132/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGT  >  NC_008463/2730537‑2730823

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ggCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATa                                                                                                                                          >  1:8674480/1‑151 (MQ=255)

ggCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATa                                                                                                                                          >  1:9029508/1‑151 (MQ=255)

  ccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAgg                                                                                                                                        <  1:2987205/151‑1 (MQ=255)

    gccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                      <  1:437252/151‑1 (MQ=255)

       tGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                   >  2:527342/1‑151 (MQ=255)

       tGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                   <  1:5380368/151‑1 (MQ=255)

            ggCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGc                                                                                                                              <  1:2244915/151‑1 (MQ=255)

               cGGCTGCCGCACTACGACGGCTACGAGTGGTTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAg                                                                                                                           <  2:10860545/151‑1 (MQ=255)

                  cTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGcc                                                                                                                        <  2:4431511/151‑1 (MQ=255)

                    gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGGGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGcccc                                                                                                                      <  2:10788539/151‑1 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGt                                                                                                                    >  2:4811456/1‑151 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGt                                                                                                                    <  2:8505969/151‑1 (MQ=255)

                       gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                   <  2:7966552/151‑1 (MQ=255)

                       gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAACGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                   >  1:4182699/1‑151 (MQ=255)

                        cacgccgacgGCTACGACTCGCTGCAGGCCGGCACCGACCGGCCGCTGGTGGCGGCGGCGACCGGGCTGAACTACGAAGTCCTGGACAACGCACACGACTGCGCGGGCCAGCGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTg                                                                                                                  <  2:5680069/146‑1 (MQ=255)

                        cACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTg                                                                                                                  >  1:2397273/1‑151 (MQ=255)

                          cTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGa                                                                                                                <  2:6656439/151‑1 (MQ=255)

                            acgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                              <  1:9846859/151‑1 (MQ=255)

                            acgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTCTCCGCCAGGCCCCGTTGGAGt                                                                                                              >  2:4960777/1‑151 (MQ=255)

                              gacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACGGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                            <  2:3640466/151‑1 (MQ=255)

                              cacgGCAACGCGTCGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGAGCTACGACGTGCTGGACACCCCTCACGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCAATCCGCCAGGCCCCGTGGGAGTgg                                                                                                            <  2:9629399/150‑1 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATGGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                          >  2:1280095/1‑151 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                          <  1:6310008/151‑1 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                          >  2:8560031/1‑151 (MQ=255)

                                  gCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                        >  1:2063593/1‑151 (MQ=255)

                                  gCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                        <  1:6619859/151‑1 (MQ=255)

                                  gCTAAGAGTGGATGCAGGCCGGCACCGACGTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGATCTCGACTGAGCGGGCCAGGGCGGGTTGCGCCATTGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGa                                                                                                        <  2:10461041/151‑1 (MQ=255)

                                    tACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATa                                                                                                      <  2:42270/151‑1 (MQ=255)

                                      cGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGGTGGAGTGGCGGATAAc                                                                                                    >  1:1938884/1‑151 (MQ=255)

                                       gAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAcc                                                                                                   >  2:382185/1‑151 (MQ=255)

                                       gAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAcc                                                                                                   <  1:122260/151‑1 (MQ=255)

                                         gTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgt                                                                                                 <  1:3098402/151‑1 (MQ=255)

                                         gTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgt                                                                                                 >  1:1791611/1‑151 (MQ=255)

                                          tGGCTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATCGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAGAACCgtg                                                                                                <  2:10287383/151‑1 (MQ=255)

                                            gATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                              >  1:9147290/1‑151 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTg                                                                                             <  1:1099756/151‑1 (MQ=255)

                                               gCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcg                                                                                           <  1:10163558/151‑1 (MQ=255)

                                                    ccGGCACCGACCTGCTGCTGGTGGCGGTGGCGACGGGGCTGATCTTCGAAGTGGTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCTCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGt                                                                                      <  2:2160295/151‑1 (MQ=255)

                                                      ggCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTcc                                                                                    >  2:8144429/1‑151 (MQ=255)

                                                      ggCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTcc                                                                                    <  1:1376172/151‑1 (MQ=255)

                                                      ggCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTcc                                                                                    <  2:8790655/151‑1 (MQ=255)

                                                       gCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCg                                                                                   <  1:8555955/151‑1 (MQ=255)

                                                         aCCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGcc                                                                                 >  2:925189/1‑151 (MQ=255)

                                                         aCCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGcc                                                                                 >  2:6972683/1‑151 (MQ=255)

                                                           cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                               <  2:5295893/151‑1 (MQ=255)

                                                           cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                               >  1:3172153/1‑151 (MQ=255)

                                                            gACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACTAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTa                                                                              <  1:10511122/151‑1 (MQ=255)

                                                             aCCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTAc                                                                             <  2:9515626/151‑1 (MQ=255)

                                                                 gcggctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCCACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCg                                                                         <  1:4524388/148‑1 (MQ=255)

                                                                     ctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGa                                                                     <  2:3992435/151‑1 (MQ=255)

                                                                     ctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGa                                                                     <  2:4366561/151‑1 (MQ=255)

                                                                      tggtggCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGaa                                                                    <  2:3387485/151‑1 (MQ=255)

                                                                         tggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGCCTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGACGTc                                                                 <  1:1159620/151‑1 (MQ=255)

                                                                         tggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTc                                                                 >  1:7220553/1‑151 (MQ=255)

                                                                           gcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCt                                                               <  2:2447154/151‑1 (MQ=255)

                                                                             ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             >  1:5243293/1‑151 (MQ=255)

                                                                             ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             <  1:1574597/151‑1 (MQ=255)

                                                                             ggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCttt                                                             >  2:1519493/1‑151 (MQ=255)

                                                                              gtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCtttt                                                            <  2:6112881/151‑1 (MQ=255)

                                                                               tggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATCACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTc                                                           <  2:3230009/151‑1 (MQ=255)

                                                                               tggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTc                                                           <  1:9194012/151‑1 (MQ=255)

                                                                                ggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCt                                                          <  1:4824988/151‑1 (MQ=255)

                                                                                     ccGGGCTGATCTACGAAGTGCTGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCATTTCTTAccc                                                     <  1:768774/151‑1 (MQ=255)

                                                                                     ccGGGCTGATCTACGAAGAGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAccc                                                     <  1:9698289/151‑1 (MQ=255)

                                                                                        ggCTGATCTACGACGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAg                                                  <  1:3685844/151‑1 (MQ=255)

                                                                                        ggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAg                                                  <  1:8225050/151‑1 (MQ=255)

                                                                                         gCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAgg                                                 <  2:2826021/151‑1 (MQ=255)

                                                                                           tGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTATCGCCATAGGCGCTATCCGCCAGGCCCAGTTGGAGTGGCGGATAACCGTGATCCGCTTTTCCGCCCAACCTCGTGGAAGACCTTTTCTTACCCCAGGTc                                               >  1:3274527/1‑151 (MQ=255)

                                                                                            gATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCa                                              <  1:5458716/151‑1 (MQ=255)

                                                                                             aTCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAg                                             <  1:6543491/151‑1 (MQ=255)

                                                                                                   gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGc                                       <  1:6823666/151‑1 (MQ=255)

                                                                                                   gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGc                                       >  1:7346899/1‑151 (MQ=255)

                                                                                                    aaGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCa                                      <  1:10258828/151‑1 (MQ=255)

                                                                                                    aaGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCa                                      <  1:4813047/151‑1 (MQ=255)

                                                                                                       tGCTGGACAACGCTCTCGACTGTGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGc                                   >  1:3855719/1‑151 (MQ=255)

                                                                                                       tGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGc                                   <  2:9423588/151‑1 (MQ=255)

                                                                                                          tGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGtt                                >  2:7496744/1‑151 (MQ=255)

                                                                                                           ggACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTg                               >  1:3539710/1‑151 (MQ=255)

                                                                                                               aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAgg                           <  2:583975/151‑1 (MQ=255)

                                                                                                                        gACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGccc                  >  2:6471540/1‑151 (MQ=255)

                                                                                                                           tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATACCCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               <  1:4700151/151‑1 (MQ=255)

                                                                                                                           tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               <  1:4609339/151‑1 (MQ=255)

                                                                                                                           tGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGt               >  1:5817218/1‑151 (MQ=255)

                                                                                                                            gAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGtt              >  2:10680785/1‑151 (MQ=255)

                                                                                                                             aGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTg             <  1:2663134/151‑1 (MQ=255)

                                                                                                                             aGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTg             <  2:6267919/151‑1 (MQ=255)

                                                                                                                              gCGGGCCAGGGCGGGTAGCGCCATCGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgc            <  2:1892129/151‑1 (MQ=255)

                                                                                                                              gCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgc            <  1:8600559/151‑1 (MQ=255)

                                                                                                                              gCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgc            <  1:10400174/151‑1 (MQ=255)

                                                                                                                               cGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcg           >  2:2646686/1‑151 (MQ=255)

                                                                                                                                gggCCTGGGCGGGTAGCGCCATCGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGTAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcgc          <  1:10567432/151‑1 (MQ=255)

                                                                                                                                gggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcgc          >  1:7641528/1‑151 (MQ=255)

                                                                                                                                 ggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTGTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCCTCGCCCAGTTGcgcg         >  1:7528504/1‑151 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCgg        <  1:6020165/151‑1 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCgg        <  1:8200252/151‑1 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCACAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCgg        >  1:8700327/1‑151 (MQ=255)

                                                                                                                                    cAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgag      >  2:7045938/1‑151 (MQ=255)

                                                                                                                                    cAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgag      >  2:5560640/1‑151 (MQ=255)

                                                                                                                                       ggcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAgg   >  1:1465485/1‑151 (MQ=255)

                                                                                                                                       ggcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAgg   <  2:9872505/151‑1 (MQ=255)

||

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGGT  >  NC_008463/2730537‑2730823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`