Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,150,987 Δ1 bp intergenic (‑192/+24) pvcA ← / ← PA14_RS14420 paerucumarin biosynthesis protein PvcA/asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,150,9850C.99.8% 2033.9 / ‑3.4 411intergenic (‑190/+26)pvcA/PA14_RS14420paerucumarin biosynthesis protein PvcA/asparaginase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (224/186);  minor base T (1/0);  total (225/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131
                                                                                                                                                      |                                                                                                                                                  
ggCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGc                                                                                                                                                    >  2:10997336/1‑151 (MQ=255)
    gCGGAAGGCGCCATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccgcc                                                                                                                                                <  2:3723000/151‑4 (MQ=255)
      ggAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcgg                                                                                                                                             >  2:1286275/1‑151 (MQ=255)
         aGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcggcgg                                                                                                                                          >  2:6321980/1‑151 (MQ=255)
         aGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcggcgg                                                                                                                                          <  1:3990419/151‑1 (MQ=255)
            cgtcATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACCGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCggcgggcg                                                                                                                                       >  1:8751066/4‑151 (MQ=255)
            cGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCggcgggcg                                                                                                                                       >  1:8523947/1‑151 (MQ=255)
             gACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGGTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGgcgggcgg                                                                                                                                      >  2:2773325/1‑151 (MQ=255)
               cATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGcc                                                                                                                                    <  1:8985462/151‑1 (MQ=255)
               cATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGcc                                                                                                                                    <  1:8037551/151‑1 (MQ=255)
               cATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGcc                                                                                                                                    <  1:5181513/151‑1 (MQ=255)
                  ccTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGTAAACACTGTTCGCTGAAGATTTACGATGGGCGCCGGGCAGGGGCGGGCCGCCGGCGTTGTCGCGTATCGGGGGGAGCTCGGAAGTAAGCGG‑CCGCCGGCGGGCGGCCGCa                                                                                                                                 <  1:1017516/151‑1 (MQ=255)
                    tgtgGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAgg                                                                                                                               >  1:5047003/1‑151 (MQ=255)
                     gtgGACTCGCCGCCGGGGGATAAACCGGGGTAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAggg                                                                                                                              <  2:6533832/151‑1 (MQ=255)
                          cTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAGGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTc                                                                                                                         >  2:827587/1‑151 (MQ=255)
                           tCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCa                                                                                                                        >  2:9698250/1‑151 (MQ=255)
                            cgccgcCGGGGGATAAACCGGGGACGGCCGGAAACACGGTTCGCTGATGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGAATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAg                                                                                                                       <  2:8528297/151‑1 (MQ=255)
                             gccgccGGGGGATAAACCGGGGCAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  1:7742027/1‑151 (MQ=255)
                             gccgccGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  1:2858143/1‑151 (MQ=255)
                             gccgccGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  1:10383181/1‑151 (MQ=255)
                              ccgccgGGGGATAAACCGGGGATGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTc                                                                                                                     <  2:8937721/151‑1 (MQ=255)
                              ccgccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTc                                                                                                                     <  2:7067556/151‑1 (MQ=255)
                                gccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCgg                                                                                                                   >  1:3672613/1‑151 (MQ=255)
                                 ccgGGGGATCAACGGGGGAAGGCCGGAAACACGGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTGGGAATGACGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGc                                                                                                                  <  1:5824099/151‑1 (MQ=255)
                                   gggggATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGt                                                                                                                >  1:9268011/1‑151 (MQ=255)
                                       gATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTtctc                                                                                                            <  2:10537432/151‑1 (MQ=255)
                                       gATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTtctc                                                                                                            <  1:4418006/151‑1 (MQ=255)
                                       gATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTtctc                                                                                                            >  2:2396248/1‑151 (MQ=255)
                                          aaaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgcc                                                                                                         >  2:4169600/1‑151 (MQ=255)
                                          aaaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgcc                                                                                                         >  2:7194452/1‑151 (MQ=255)
                                          aaaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCGTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGCCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTACTcgcc                                                                                                         <  1:2300132/151‑1 (MQ=255)
                                           aaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgccg                                                                                                        >  1:2178477/1‑151 (MQ=255)
                                           aaCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgccg                                                                                                        >  1:1317294/1‑151 (MQ=255)
                                            aCCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgccgc                                                                                                       >  1:9359989/1‑151 (MQ=255)
                                             ccGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGca                                                                                                      <  2:1654868/151‑1 (MQ=255)
                                              cGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGTCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGcac                                                                                                     >  1:2667208/1‑151 (MQ=255)
                                              cGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGcac                                                                                                     >  2:3760967/1‑151 (MQ=255)
                                                gggAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAg                                                                                                   >  2:3030750/1‑151 (MQ=255)
                                                      gCCGGAAACACGGGTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAg                                                                                             <  2:9555432/151‑1 (MQ=255)
                                                       cctgAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTGGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAgg                                                                                            <  2:518357/148‑1 (MQ=255)
                                                        cGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAggg                                                                                           >  2:766601/1‑151 (MQ=255)
                                                         gtaaaCACTGTTCGCTGAAGCTTGACGTTGGGCGCCGGGCACGGGCGGGCCGCGGGGGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          <  2:8577459/149‑1 (MQ=255)
                                                         ggAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          >  1:11092554/1‑151 (MQ=255)
                                                         ggAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          <  1:868353/151‑1 (MQ=255)
                                                         ggAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          >  2:1936888/1‑151 (MQ=255)
                                                          gAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCg                                                                                         <  2:7831810/151‑1 (MQ=255)
                                                            aaCACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGaa                                                                                       >  1:9249826/1‑151 (MQ=255)
                                                              cacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAcc                                                                                     >  1:8389721/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    >  2:5592951/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    <  1:4306777/151‑1 (MQ=255)
                                                                     cGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGTAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTg                                                                              >  1:3456151/1‑151 (MQ=255)
                                                                     cGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTg                                                                              >  2:7941223/1‑151 (MQ=255)
                                                                     cGCTGAAGCTTGACGATGCGCGCCGGGCACGTGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGACCGCGGAAGGAAGCGG‑CAGCCGGAGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGtgg                                                                              >  1:6452654/1‑149 (MQ=255)
                                                                      gCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgc                                                                             >  1:295933/1‑151 (MQ=255)
                                                                      gCTGAAGCTTGACGAGGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGATGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgc                                                                             <  1:5640517/151‑1 (MQ=255)
                                                                       cTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGACCCAGCGTTgcg                                                                            >  2:1149374/1‑151 (MQ=255)
                                                                       cTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgcg                                                                            <  2:1801341/151‑1 (MQ=255)
                                                                         gAAGCTTGACGCTGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGGGGATCGCGGGGAGCTCGGCAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGGCAGTCGGCGTGCTCGCCGCACAGGTCGGGGGCGAACCAGCGTTGCGCt                                                                          >  2:5925275/1‑151 (MQ=255)
                                                                          aaGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGGCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgcgcgt                                                                         >  1:1699887/1‑149 (MQ=255)
                                                                           aGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtc                                                                        >  1:8129536/1‑151 (MQ=255)
                                                                           aGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtc                                                                        <  1:6847424/151‑1 (MQ=255)
                                                                             cTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgt                                                                      <  2:10100382/151‑1 (MQ=255)
                                                                             cTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgt                                                                      >  1:6210106/1‑151 (MQ=255)
                                                                                gACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgc                                                                   >  1:4729714/1‑151 (MQ=255)
                                                                                 aCGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgcg                                                                  >  1:7181202/1‑151 (MQ=255)
                                                                                  cGAAGGGCCCCGGGGACGGGCGGGCCGCCGGCGTTGACGCGTATCGCGGGGAGCACGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGCGGGCGTACCAGCGTTGCGCTTCGTCGCGt                                                                 <  2:8659274/151‑1 (MQ=255)
                                                                                      gggCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGa                                                             <  2:3504463/151‑1 (MQ=255)
                                                                                         cgcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGc                                                          <  1:7084774/151‑1 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         <  2:6784736/151‑1 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         >  1:1778125/1‑151 (MQ=255)
                                                                                           ccGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCg                                                        <  1:3560611/151‑1 (MQ=255)
                                                                                           ccGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCg                                                        >  1:4924232/1‑151 (MQ=255)
                                                                                              ggCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgcg                                                     <  2:756118/151‑1 (MQ=255)
                                                                                               gCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgcgc                                                    >  2:4857719/1‑151 (MQ=255)
                                                                                                   gggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAg                                                <  2:927366/151‑1 (MQ=255)
                                                                                                   gggcgggcCGCCGGCGTTGTCGCGGATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAg                                                >  1:1423878/1‑151 (MQ=255)
                                                                                                      cgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAg                                             >  2:6967228/1‑151 (MQ=255)
                                                                                                          ccgccgGCGTGGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTACTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCg                                         >  1:10797496/1‑151 (MQ=255)
                                                                                                            gccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGGTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGaa                                       <  2:2201790/151‑1 (MQ=255)
                                                                                                             ccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAag                                      >  2:5264347/1‑151 (MQ=255)
                                                                                                             ccgGCGTTGTCGCGTATCGCGGGGAGCACGGAAGGAAGCGG‑CCGACGTCTGGCGGCCGCAGGGCGGACAGTCGGCGTACTCTCCGCACAGGTCGAGGGCGAACCAGCGATGCGCTTCGTCGCGTCCGAGCCCGGCGCCGAGCAGGCCCAag                                      >  2:2896082/1‑151 (MQ=255)
                                                                                                              cgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAaga                                     >  1:10311258/1‑151 (MQ=255)
                                                                                                                 cGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCCGGCCGAAGAGCt                                  <  2:4438225/151‑1 (MQ=255)
                                                                                                                   ttGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTg                                >  1:3169228/1‑151 (MQ=255)
                                                                                                                      tCGCGTATCGCGGGGAGCTCGGAAGGACGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTGCTCGCCGCACAGGTCGCGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGccc                             <  2:8904583/151‑1 (MQ=255)
                                                                                                                      tCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGccc                             <  2:4978832/151‑1 (MQ=255)
                                                                                                                       cgcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCa                            <  2:4576315/151‑1 (MQ=255)
                                                                                                                       cgcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCa                            <  1:7569447/151‑1 (MQ=255)
                                                                                                                       cgcgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCa                            <  1:7879591/151‑1 (MQ=255)
                                                                                                                           tATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgc                        >  1:3308011/1‑151 (MQ=255)
                                                                                                                           tATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgc                        >  1:9907172/1‑151 (MQ=255)
                                                                                                                             tCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgccg                      <  1:7368733/151‑1 (MQ=255)
                                                                                                                               ggggggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCGTCGTCGCGGCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgccgcc                    >  2:212341/3‑151 (MQ=255)
                                                                                                                               gcgGGGAGCGCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCCCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGATGGCGAACCACCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgccccc                    >  2:8374351/1‑151 (MQ=255)
                                                                                                                                cgGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCt                   >  1:5442315/1‑151 (MQ=255)
                                                                                                                                 ggggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTc                  <  1:6544328/151‑1 (MQ=255)
                                                                                                                                    gAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTcgcg               <  1:10020373/151‑1 (MQ=255)
                                                                                                                                       cTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCgggg            >  2:10692303/1‑150 (MQ=255)
                                                                                                                                         cGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTAGGCGTTCTCGCCTCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGACCAGGGCCGCCTCGCGGGTCa          >  1:5479332/1‑151 (MQ=255)
                                                                                                                                                 agCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTccacca  >  1:10918502/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑