Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,146 Δ1 bp coding (230/1980 nt) PA14_RS14610 ← cation/acetate symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,1440G.98.8% 2042.3 / 9.7 421coding (232/1980 nt)PA14_RS14610cation/acetate symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (221/195);  minor base C (2/3);  total (223/198)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290
                                                                                                                                                      |                                                                                                                                                  
 gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAgg                                                                                                                                                   <  2:10111339/151‑1 (MQ=255)
  ggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCCGAGCCTGGGTCAGTCGATCGAATTCGCCGTGGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                  <  2:1482389/151‑2 (MQ=255)
    cGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcac                                                                                                                                                <  2:1291199/151‑4 (MQ=255)
    cGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcac                                                                                                                                                >  2:10279950/1‑148 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               >  1:4219029/1‑147 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               >  2:3488669/1‑147 (MQ=255)
         ccGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaag                                                                                                                                          <  2:3317829/151‑1 (MQ=255)
         ccGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaag                                                                                                                                          <  2:468050/151‑1 (MQ=255)
         ccGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaag                                                                                                                                          >  1:2556469/1‑151 (MQ=255)
           gACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAgaagaa                                                                                                                                        >  1:3177898/1‑151 (MQ=255)
            aCAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAac                                                                                                                                       >  2:9240339/1‑151 (MQ=255)
             cagcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAaca                                                                                                                                      >  1:10025559/1‑151 (MQ=255)
              agcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAacac                                                                                                                                     <  2:3835957/151‑1 (MQ=255)
                 agGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGaa                                                                                                                                  >  2:839378/1‑151 (MQ=255)
                 agGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGaa                                                                                                                                  >  2:7704047/1‑151 (MQ=255)
                  ggCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAAc                                                                                                                                 <  1:5494783/151‑1 (MQ=255)
                      ggAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACCA‑GGCACAGAAGAACACGAACAGGa                                                                                                                             >  2:8469813/1‑151 (MQ=255)
                          tGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAacac                                                                                                                         <  1:10198958/151‑1 (MQ=255)
                          tGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAacac                                                                                                                         <  1:1529425/151‑1 (MQ=255)
                           gCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACg                                                                                                                        >  2:2098844/1‑151 (MQ=255)
                            cAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                       >  1:7918202/1‑151 (MQ=255)
                              gCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccg                                                                                                                     >  1:2094803/1‑151 (MQ=255)
                              gCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccg                                                                                                                     <  2:2592908/151‑1 (MQ=255)
                              gCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccg                                                                                                                     <  1:6242011/151‑1 (MQ=255)
                                 aCAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                  <  2:5493521/151‑1 (MQ=255)
                                  cAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggccg                                                                                                                 >  1:3865638/1‑151 (MQ=255)
                                  cAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggccg                                                                                                                 >  1:443197/1‑151 (MQ=255)
                                       ccAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcc                                                                                                            >  2:4146052/1‑151 (MQ=255)
                                       ccAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcc                                                                                                            <  2:8843815/151‑1 (MQ=255)
                                         aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcccc                                                                                                          >  1:7651603/1‑151 (MQ=255)
                                          ggggAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCg                                                                                                         <  1:5130043/151‑1 (MQ=255)
                                             gAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAg                                                                                                      >  2:8620081/1‑151 (MQ=255)
                                             gAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAg                                                                                                      >  1:7537078/1‑151 (MQ=255)
                                                ggAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGACCACGCCGGCCGCGACCCCGAAGGTc                                                                                                   >  2:42845/1‑151 (MQ=255)
                                                ggAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTc                                                                                                   <  2:3420731/151‑1 (MQ=255)
                                                 gAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGAC‑ACGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCa                                                                                                  >  1:8516239/1‑151 (MQ=255)
                                                  aGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAt                                                                                                 >  1:3439713/1‑151 (MQ=255)
                                                    cttcttCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGt                                                                                               <  2:8734567/151‑1 (MQ=255)
                                                      tcttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTc                                                                                             <  1:10564286/151‑1 (MQ=255)
                                                      tcttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTc                                                                                             <  1:4782873/151‑1 (MQ=255)
                                                       cttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCg                                                                                            >  1:8668908/1‑151 (MQ=255)
                                                          cATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                         >  2:8277261/1‑151 (MQ=255)
                                                          cATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                         >  2:1670144/1‑151 (MQ=255)
                                                          cATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                         <  1:3286261/151‑1 (MQ=255)
                                                          cATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                         <  1:8239346/151‑1 (MQ=255)
                                                              cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                     <  1:8053255/151‑1 (MQ=255)
                                                              cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                     >  2:9002807/1‑151 (MQ=255)
                                                               gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                    <  1:1471021/151‑1 (MQ=255)
                                                                   cGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCa                                                                                <  1:361908/151‑1 (MQ=255)
                                                                   cGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATACAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCa                                                                                <  2:2317645/151‑1 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                               >  1:1761830/1‑151 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                               >  1:8694922/1‑151 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                               >  1:1254197/1‑151 (MQ=255)
                                                                         tctTCCAGCCCAGCGTCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGcc                                                                          <  1:3524773/151‑1 (MQ=255)
                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTCCACATAGAGCGCCGAC‑ACGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGGTCGTGCCCTGCGCCAGACGcc                                                                          >  2:6635462/1‑151 (MQ=255)
                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGTCGcc                                                                          <  2:7356064/151‑1 (MQ=255)
                                                                            tCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTgg                                                                       <  1:1566458/151‑1 (MQ=255)
                                                                              cagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCa                                                                     >  1:8361355/1‑151 (MQ=255)
                                                                              cagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGACCTGCGCCAGACGCCTGGCa                                                                     >  2:9742915/1‑151 (MQ=255)
                                                                               agcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCaa                                                                    >  2:570037/1‑151 (MQ=255)
                                                                                 cccagcGACCAGAGCCTGGGTCCGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGATCACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCGGGCAATc                                                                  >  2:11075215/1‑151 (MQ=255)
                                                                                  ccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCa                                                                 >  1:3233362/1‑151 (MQ=255)
                                                                                    agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                               >  1:9575118/1‑151 (MQ=255)
                                                                                      cGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCACTCAGCTc                                                             >  1:6697419/1‑151 (MQ=255)
                                                                                       gACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCg                                                            <  2:6063494/151‑1 (MQ=255)
                                                                                       gACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCg                                                            >  1:9748362/1‑151 (MQ=255)
                                                                                        aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGTACACGACCAGGTACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGTCGCCTGGCActcggctcgg                                                           >  1:5927274/1‑151 (MQ=255)
                                                                                        aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                           >  1:8328051/1‑151 (MQ=255)
                                                                                          cAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgggg                                                         <  2:7943411/151‑1 (MQ=255)
                                                                                          cAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgggg                                                         >  2:493417/1‑151 (MQ=255)
                                                                                           agagCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGc                                                        >  1:2847007/1‑151 (MQ=255)
                                                                                              gCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGa                                                     >  2:1089061/1‑151 (MQ=255)
                                                                                                cTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAAc                                                   <  1:3926177/151‑1 (MQ=255)
                                                                                                     tCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAc                                              <  1:4669364/151‑1 (MQ=255)
                                                                                                      cAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAcc                                             >  1:8293817/1‑151 (MQ=255)
                                                                                                      cAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAcc                                             >  1:7419115/1‑151 (MQ=255)
                                                                                                        ggCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAg                                           <  2:10402185/151‑1 (MQ=255)
                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                        >  2:7070532/1‑151 (MQ=255)
                                                                                                              cGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAGGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGag                                     >  2:9896951/1‑150 (MQ=255)
                                                                                                                aaTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGt                                   >  1:7315463/1‑151 (MQ=255)
                                                                                                                 aTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTa                                  <  1:11037111/151‑1 (MQ=255)
                                                                                                                    cGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGcc                               <  2:6876431/151‑1 (MQ=255)
                                                                                                                      ccGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAAGGTCGAGCCATGCGACTGACGCCTGGCACACAGCGAGGGTCAGAACGATACCAGGGCGATGAAGCCGt                             >  2:368042/1‑151 (MQ=255)
                                                                                                                       cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTg                            <  1:9127939/151‑1 (MQ=255)
                                                                                                                        gTTGGCGCGTTACACATAGATCGCCGACAA‑GGCACAGAAGAACACTAACACGAACACGCCTGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAGACCAGCGCGATGTAGCCGTgg                           <  2:9494057/151‑1 (MQ=255)
                                                                                                                           ggCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAAc                        >  1:8838917/1‑151 (MQ=255)
                                                                                                                            gcgcGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACg                       >  1:5596964/1‑151 (MQ=255)
                                                                                                                             cgcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGt                      >  1:3683876/1‑151 (MQ=255)
                                                                                                                              gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                     <  1:493424/151‑1 (MQ=255)
                                                                                                                                  tACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCCGCGCGATGTAGCCGTGGAACGTGGCCa                 >  2:6139657/1‑151 (MQ=255)
                                                                                                                                  tACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCa                 <  1:9393926/151‑1 (MQ=255)
                                                                                                                                   acacATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAg                >  2:9336430/1‑151 (MQ=255)
                                                                                                                                       aTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc            <  2:10003038/151‑1 (MQ=255)
                                                                                                                                       aTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc            <  2:8208241/151‑1 (MQ=255)
                                                                                                                                        tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca           >  2:3085271/1‑151 (MQ=255)
                                                                                                                                        tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca           >  2:6712385/1‑151 (MQ=255)
                                                                                                                                         agagCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcag          >  2:2827677/1‑151 (MQ=255)
                                                                                                                                             cgcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagg      >  2:4412793/1‑151 (MQ=255)
                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga     >  2:5322942/1‑151 (MQ=255)
                                                                                                                                                cGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc   <  2:5139860/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑