Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,3570AG100.0% 1668.8 / NA 421L314L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (212/209);  total (212/209)

Only 100 of 421 total aligned reads displayed.

CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504
                                                                                                                                                    |                                                                                                                                                   
cAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGc                                                                                                                                                   <  2:8986188/151‑1 (MQ=255)
     cagcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtg                                                                                                                                              <  2:7563045/151‑1 (MQ=255)
      agcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                             >  1:4160321/1‑151 (MQ=255)
      agcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                             >  1:4709204/1‑151 (MQ=255)
         agGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                          >  1:2336527/1‑151 (MQ=255)
         agGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                          >  1:9228712/1‑151 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  1:1564241/151‑1 (MQ=255)
          ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                         <  2:7375418/151‑1 (MQ=255)
           gCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCg                                                                                                                                        >  2:1612474/1‑151 (MQ=255)
           gCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCg                                                                                                                                        <  1:6735411/151‑1 (MQ=255)
             cGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGAt                                                                                                                                      >  2:1785739/1‑151 (MQ=255)
              gCTGAGCACCATCCTGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATc                                                                                                                                     <  1:9248423/151‑1 (MQ=255)
                tGAGCACCATCATTCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGTTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   <  2:5065561/151‑1 (MQ=255)
                tGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                   >  1:3912743/1‑151 (MQ=255)
                 gAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTg                                                                                                                                  >  1:1884604/1‑151 (MQ=255)
                  aGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGc                                                                                                                                 >  2:8589054/1‑151 (MQ=255)
                    cACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCg                                                                                                                               >  1:7009539/1‑151 (MQ=255)
                     aCCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCgg                                                                                                                              >  1:10580672/1‑151 (MQ=255)
                       catcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                            <  1:3632230/151‑1 (MQ=255)
                       catcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                            <  2:1413268/151‑1 (MQ=255)
                         tcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtata                                                                                                                          <  2:6628395/151‑1 (MQ=255)
                                 agcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATTAGGGTTCa                                                                                                                  <  1:6876079/151‑1 (MQ=255)
                                 agcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCa                                                                                                                  <  2:4256420/151‑1 (MQ=255)
                                 agcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCa                                                                                                                  >  1:1444287/1‑151 (MQ=255)
                                  gcagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAg                                                                                                                 <  1:9861845/151‑1 (MQ=255)
                                   cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATCCCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                                <  2:961828/151‑1 (MQ=255)
                                   cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                                <  2:7051740/151‑1 (MQ=255)
                                   cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                                <  2:5817752/151‑1 (MQ=255)
                                    agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgcg                                                                                                               >  1:5487238/1‑151 (MQ=255)
                                     gcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgcgc                                                                                                              <  1:8137499/151‑1 (MQ=255)
                                       agGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCgg                                                                                                            >  1:10933039/1‑151 (MQ=255)
                                          ccGAAGACGAACCAGCTCAGCGTGACCCAGATACCGCCGAACTCGCCGGTGTGCAGGGGACGCTTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCg                                                                                                         <  2:602735/151‑1 (MQ=255)
                                           cGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATATTTCCGGTATAAGGGTTCAGCGCGGCCGa                                                                                                        >  1:6015130/1‑151 (MQ=255)
                                            gAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGAc                                                                                                       >  1:2580680/1‑151 (MQ=255)
                                            gAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGAc                                                                                                       >  2:5608730/1‑151 (MQ=255)
                                               gACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTgg                                                                                                    <  2:8846488/151‑1 (MQ=255)
                                                  gAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAAc                                                                                                 >  1:7320066/1‑151 (MQ=255)
                                                      cAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGa                                                                                             >  1:6482607/1‑151 (MQ=255)
                                                       aGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAg                                                                                            <  2:10986845/151‑1 (MQ=255)
                                                         aTCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCg                                                                                          <  2:7152778/151‑1 (MQ=255)
                                                            aGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGAt                                                                                       <  1:8458123/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      <  2:1129745/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      >  2:817296/1‑151 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      <  2:2619175/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      <  1:5606095/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      <  1:7592288/151‑1 (MQ=255)
                                                             gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATa                                                                                      >  2:6247846/1‑151 (MQ=255)
                                                               ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAcc                                                                                    <  1:8001049/151‑1 (MQ=255)
                                                                 gACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAg                                                                                  >  1:6668485/1‑151 (MQ=255)
                                                                    ccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCcg                                                                               >  1:87355/1‑151 (MQ=255)
                                                                          aCCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTccg                                                                         <  2:10542276/151‑2 (MQ=255)
                                                                             gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                      <  1:511226/151‑1 (MQ=255)
                                                                             gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                      >  2:783139/1‑151 (MQ=255)
                                                                             gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                      <  1:1747353/151‑1 (MQ=255)
                                                                              ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                     <  1:1872873/151‑1 (MQ=255)
                                                                                  aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                 >  2:4510499/1‑151 (MQ=255)
                                                                                    ccGCCGGTGTGCAGGGGACGCATGGAGTCGGTGACGAACAGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGACCa                                                               <  1:9567241/150‑1 (MQ=255)
                                                                                      gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                             <  2:5242622/151‑1 (MQ=255)
                                                                                      gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                             <  1:7958482/151‑1 (MQ=255)
                                                                                       ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                            >  2:10295917/1‑151 (MQ=255)
                                                                                         ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                          >  2:7983183/1‑151 (MQ=255)
                                                                                          gtgtGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                         >  2:3278063/1‑151 (MQ=255)
                                                                                           tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCt                                                        <  2:3649094/151‑1 (MQ=255)
                                                                                            gtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCAACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTg                                                       <  2:10098523/151‑1 (MQ=255)
                                                                                               cAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAg                                                    >  2:537739/1‑151 (MQ=255)
                                                                                                aGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAgg                                                   <  2:6559169/151‑1 (MQ=255)
                                                                                                   ggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGt                                                >  1:8973943/1‑151 (MQ=255)
                                                                                                     aCGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTg                                              >  2:3132929/1‑151 (MQ=255)
                                                                                                      cGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGc                                             >  1:584248/1‑151 (MQ=255)
                                                                                                       gCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                            >  1:10494082/1‑151 (MQ=255)
                                                                                                         aTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGACCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                          >  2:135570/1‑151 (MQ=255)
                                                                                                         aTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                          <  1:8933619/151‑1 (MQ=255)
                                                                                                           ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGc                                        <  2:1577080/151‑1 (MQ=255)
                                                                                                           ggAGTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGc                                        <  2:4079781/151‑1 (MQ=255)
                                                                                                            gATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCa                                       <  1:6510650/151‑1 (MQ=255)
                                                                                                                cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                   >  2:3864146/1‑151 (MQ=255)
                                                                                                                 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                  >  1:276088/1‑151 (MQ=255)
                                                                                                                 ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTGTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                  <  1:7499734/151‑1 (MQ=255)
                                                                                                                    gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                               >  2:6910747/1‑151 (MQ=255)
                                                                                                                      cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                             >  1:356797/1‑151 (MQ=255)
                                                                                                                      cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                             <  1:4833518/151‑1 (MQ=255)
                                                                                                                      cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                             >  1:5070718/1‑151 (MQ=255)
                                                                                                                        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                           >  1:626303/1‑151 (MQ=255)
                                                                                                                        aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                           <  2:2032793/151‑1 (MQ=255)
                                                                                                                         aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                          <  1:4957938/151‑1 (MQ=255)
                                                                                                                          cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTg                         <  2:1908224/151‑1 (MQ=255)
                                                                                                                          cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTg                         <  1:3025224/151‑1 (MQ=255)
                                                                                                                              aGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                     >  1:8431925/1‑151 (MQ=255)
                                                                                                                               ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                    <  2:7689888/151‑1 (MQ=255)
                                                                                                                                    gTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGc               >  2:2144743/1‑151 (MQ=255)
                                                                                                                                     tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTCTAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt              >  1:3095056/1‑151 (MQ=255)
                                                                                                                                        ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg           >  1:10269610/1‑151 (MQ=255)
                                                                                                                                            gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa       >  1:2206649/1‑151 (MQ=255)
                                                                                                                                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc      <  2:6482607/151‑1 (MQ=255)
                                                                                                                                             cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc      <  2:6054854/151‑1 (MQ=255)
                                                                                                                                              tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg     <  1:7166294/151‑1 (MQ=255)
                                                                                                                                                aGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGcc   <  2:2291250/151‑1 (MQ=255)
                                                                                                                                                 gCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGccc  <  2:2896229/151‑1 (MQ=255)
                                                                                                                                                 gCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGccc  <  1:4452590/151‑1 (MQ=255)
                                                                                                                                                 gCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGccc  >  2:4342084/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCC  >  NC_008463/2955209‑2955504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑