BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,281,477	G→C	intergenic (‑25/+44)	katE ← / ← PA14_RS14985	catalase HPII/general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,281,477	0	G	C	100.0%	1400.2 / NA	357	intergenic (‑25/+44)	katE/PA14_RS14985	catalase HPII/general stress protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (173/184); total (173/184)

Only 100 of 361 total aligned reads displayed.

TGGCGTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGGGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTGC  >  NC_008463/3281329‑3281624

   cGTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATg                                                                                                                                                <  1:10159234/151‑1 (MQ=255)

     tCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGcc                                                                                                                                              <  1:4483465/151‑1 (MQ=255)

      ccTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCt                                                                                                                                             <  2:10951494/151‑1 (MQ=255)

       cTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTc                                                                                                                                            >  1:10457386/1‑151 (MQ=255)

       cTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTc                                                                                                                                            >  1:3017555/1‑151 (MQ=255)

        tCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTcc                                                                                                                                           <  1:3476426/151‑1 (MQ=255)

          gcgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCt                                                                                                                                         <  2:3713290/151‑1 (MQ=255)

           cgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTg                                                                                                                                        >  1:3777549/1‑151 (MQ=255)

           cgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTg                                                                                                                                        >  1:1810490/1‑151 (MQ=255)

           cgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTg                                                                                                                                        >  2:1603987/1‑151 (MQ=255)

           cgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGGTGCGACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGGTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTg                                                                                                                                        <  2:873523/151‑1 (MQ=255)

             aTAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCt                                                                                                                                      <  1:10018963/151‑1 (MQ=255)

             aTAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCt                                                                                                                                      >  1:6498612/1‑151 (MQ=255)

               aGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTcc                                                                                                                                    >  1:2215460/1‑151 (MQ=255)

               aGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTcc                                                                                                                                    <  2:10989606/151‑1 (MQ=255)

                 gCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGc                                                                                                                                  >  1:2257070/1‑151 (MQ=255)

                  ccTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGcc                                                                                                                                 >  1:1448301/1‑151 (MQ=255)

                         cTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttca                                                                                                                          <  1:401951/151‑1 (MQ=255)

                           gTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcact                                                                                                                        >  1:10074575/1‑151 (MQ=255)

                            ttCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcactt                                                                                                                       >  1:2744900/1‑151 (MQ=255)

                                aGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATtcacttcact                                                                                                                   <  2:1121588/151‑1 (MQ=255)

                                aGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATtcacttcact                                                                                                                   <  2:2834000/151‑1 (MQ=255)

                                 gCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTg                                                                                                                  <  2:6347271/151‑1 (MQ=255)

                                 gCTTGGCGTTGCTGGTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTg                                                                                                                  <  1:9513559/151‑1 (MQ=255)

                                  cTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTgc                                                                                                                 <  1:9239117/151‑1 (MQ=255)

                                   ttGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTgcg                                                                                                                <  1:2372873/151‑1 (MQ=255)

                                         ttGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTa                                                                                                          >  2:4044688/1‑151 (MQ=255)

                                         ttGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTa                                                                                                          <  1:3177000/151‑1 (MQ=255)

                                          tGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTAt                                                                                                         <  1:3581841/151‑1 (MQ=255)

                                          tGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTAt                                                                                                         >  2:7014864/1‑151 (MQ=255)

                                          tGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTAt                                                                                                         >  2:8186806/1‑151 (MQ=255)

                                             tGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAg                                                                                                      >  2:685104/1‑151 (MQ=255)

                                               ttGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGtt                                                                                                    <  2:4725037/151‑1 (MQ=255)

                                               ttGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGtt                                                                                                    >  2:7723759/1‑151 (MQ=255)

                                                 gCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCACATCCTGGGCATGACAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTcc                                                                                                  >  2:4330644/1‑151 (MQ=255)

                                                 gCCACGGTCGACGGTGTCGGGGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTcc                                                                                                  <  2:9676316/151‑1 (MQ=255)

                                                    aCGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgc                                                                                               >  2:6945564/1‑151 (MQ=255)

                                                     cGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgcc                                                                                              <  1:6760779/151‑1 (MQ=255)

                                                     cGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCAGCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgcc                                                                                              >  2:2013706/1‑151 (MQ=255)

                                                        tCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCAGCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTg                                                                                           <  2:9389873/151‑1 (MQ=255)

                                                         cGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTgg                                                                                          <  2:2668683/151‑1 (MQ=255)

                                                           aCGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCt                                                                                        <  2:6637388/151‑1 (MQ=255)

                                                                 tCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTg                                                                                  <  1:6147306/151‑1 (MQ=255)

                                                                 tCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTg                                                                                  <  1:943349/151‑1 (MQ=255)

                                                                    gTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGcc                                                                               <  2:822347/151‑1 (MQ=255)

                                                                     tGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCa                                                                              >  1:2635013/1‑151 (MQ=255)

                                                                     tGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATGCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCa                                                                              >  2:473680/1‑151 (MQ=255)

                                                                         ggccgCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCtt                                                                          <  1:9061968/151‑1 (MQ=255)

                                                                           ccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTc                                                                        >  1:168011/1‑151 (MQ=255)

                                                                           ccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTc                                                                        <  1:11011467/151‑1 (MQ=255)

                                                                            cgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCt                                                                       >  1:6600984/1‑151 (MQ=255)

                                                                              ccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTg                                                                     <  2:7368761/151‑1 (MQ=255)

                                                                              ccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTg                                                                     <  2:716705/151‑1 (MQ=255)

                                                                               cTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGc                                                                    >  2:4518731/1‑151 (MQ=255)

                                                                                tGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGcc                                                                   <  2:9240452/151‑1 (MQ=255)

                                                                                    tgcgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGcc                                                               <  2:7994358/151‑1 (MQ=255)

                                                                                    tgcgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGcc                                                               <  1:4315368/151‑1 (MQ=255)

                                                                                     gcgctgcTCGTTGTTCTGCACGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCt                                                              >  2:8959329/1‑151 (MQ=255)

                                                                                      cgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTc                                                             <  1:125719/151‑1 (MQ=255)

                                                                                      cgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTc                                                             >  1:4139928/1‑151 (MQ=255)

                                                                                      cgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTc                                                             <  2:5068287/151‑1 (MQ=255)

                                                                                         tgctCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGa                                                          >  1:4809382/1‑151 (MQ=255)

                                                                                         tgctCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGa                                                          >  1:8847755/1‑151 (MQ=255)

                                                                                            tCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGc                                                       >  1:5632848/1‑151 (MQ=255)

                                                                                              gttgttCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGccc                                                     <  2:1411703/151‑1 (MQ=255)

                                                                                                   tCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGc                                                <  2:1780281/151‑1 (MQ=255)

                                                                                                    cTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCg                                               <  1:2713373/151‑1 (MQ=255)

                                                                                                        tcgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATc                                           >  2:608318/1‑151 (MQ=255)

                                                                                                        tcgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATc                                           <  2:443396/151‑1 (MQ=255)

                                                                                                         cgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCg                                          >  1:10551430/1‑151 (MQ=255)

                                                                                                             cATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCt                                      >  1:1395431/1‑151 (MQ=255)

                                                                                                              aTCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCtt                                     <  1:6444628/151‑1 (MQ=255)

                                                                                                                cGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGa                                   <  2:10736879/151‑1 (MQ=255)

                                                                                                                 ggTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTGTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGaa                                  >  2:4947461/1‑151 (MQ=255)

                                                                                                                    ttCCACTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTGCACGGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTACTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGtt                               <  2:10575039/151‑1 (MQ=255)

                                                                                                                     tcctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTg                              <  1:7563145/151‑1 (MQ=255)

                                                                                                                      cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgc                             >  1:4953222/1‑151 (MQ=255)

                                                                                                                      cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgc                             <  1:7873028/151‑1 (MQ=255)

                                                                                                                      cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgc                             <  2:7528017/151‑1 (MQ=255)

                                                                                                                       ctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgcc                            >  2:3246150/1‑151 (MQ=255)

                                                                                                                       ctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgcc                            >  1:3108557/1‑151 (MQ=255)

                                                                                                                        tctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgccg                           >  2:5357640/1‑151 (MQ=255)

                                                                                                                         ctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgccgc                          >  1:5293172/1‑151 (MQ=255)

                                                                                                                          tcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgccgcc                         >  2:8495912/1‑151 (MQ=255)

                                                                                                                           cctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGccgccg                        >  2:1266468/1‑151 (MQ=255)

                                                                                                                               aTCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctgg                    >  2:235924/1‑151 (MQ=255)

                                                                                                                                 ccTGGGCATGAAAATGTGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcc                  >  2:2571430/1‑151 (MQ=255)

                                                                                                                                 ccTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcc                  >  1:5028615/1‑151 (MQ=255)

                                                                                                                                 ccTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACGTCCCTGCGCGGTTAGCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcc                  <  2:5982755/151‑1 (MQ=255)

                                                                                                                                  cTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcct                 >  2:5282887/1‑151 (MQ=255)

                                                                                                                                  cTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcct                 >  2:5754488/1‑151 (MQ=255)

                                                                                                                                      gCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCtggcctggcc             <  1:9941173/151‑1 (MQ=255)

                                                                                                                                        aTGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTAGCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGgccgc           >  2:7450184/1‑151 (MQ=255)

                                                                                                                                          gAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGccc         >  1:9716471/1‑151 (MQ=255)

                                                                                                                                          gAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGccc         <  1:854687/151‑1 (MQ=255)

                                                                                                                                               tGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttctt    <  2:10865334/151‑1 (MQ=255)

                                                                                                                                               tGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTATCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttctt    >  1:7067618/1‑151 (MQ=255)

                                                                                                                                               tGGGGCGGATGCCGCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttctt    <  2:10499357/151‑1 (MQ=255)

                                                                                                                                                ggggcggATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTg   >  2:10189055/1‑151 (MQ=255)

                                                                                                                                                 gggcggATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTGc  >  1:8696155/1‑151 (MQ=255)

TGGCGTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGGGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTGC  >  NC_008463/3281329‑3281624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`