Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,443,2920CG99.8% 1788.8 / NA 465P459A (CCC→GCC) PA14_RS15680GntP family permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (249/215);  minor base T (0/1);  total (249/216)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTGC  >  NC_008463/3443144‑3443439
                                                                                                                                                    |                                                                                                                                                   
cggcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTAcgcc                                                                                                                                                   <  1:2806663/151‑1 (MQ=255)
 ggcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCa                                                                                                                                                  <  1:7586684/151‑1 (MQ=255)
   cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                                <  1:1954007/151‑1 (MQ=255)
    gggCTGGACCCCCTGCCGCACAACGGCGCGGTGATCACCCCCCCGGCGCTCACCGGCCTGACCCACCGCGAGGCGTACAAGGACATTTTCGGCACTACCCTGCTCAAGACCCTGGCGGTGTTCTTCGTCTTCGTTGTGTTGTACGCCACCg                                                                                                                                               <  2:1830026/151‑1 (MQ=255)
    gggATGGAGACCCTGCCGCACACCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGTCTGTTCTACGCCACCg                                                                                                                                               <  2:550171/151‑1 (MQ=255)
     ggatggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCgg                                                                                                                                              <  2:8930631/151‑1 (MQ=255)
       atggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGcc                                                                                                                                            <  1:5267343/151‑1 (MQ=255)
         ggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                          <  2:4646507/151‑1 (MQ=255)
               ccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGa                                                                                                                                    >  1:796733/1‑151 (MQ=255)
                        caACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtt                                                                                                                           <  2:9508881/151‑1 (MQ=255)
                         aaCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttt                                                                                                                          >  2:10069487/1‑151 (MQ=255)
                            ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                       <  1:7830883/151‑1 (MQ=255)
                            ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                       <  1:852387/151‑1 (MQ=255)
                               gcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCAt                                                                                                                    >  1:1398849/1‑151 (MQ=255)
                                cgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATc                                                                                                                   <  2:8391182/151‑1 (MQ=255)
                                 ggTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATcc                                                                                                                  >  1:5736423/1‑151 (MQ=255)
                                   tGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTg                                                                                                                >  2:9726402/1‑151 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                           >  1:3409277/1‑151 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                           <  2:3078098/151‑1 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                           >  2:6630363/1‑151 (MQ=255)
                                              ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                     <  1:9840257/151‑1 (MQ=255)
                                                ggCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGc                                                                                                   >  2:4184226/1‑151 (MQ=255)
                                                ggCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGc                                                                                                   <  1:10460674/151‑1 (MQ=255)
                                                  cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCAt                                                                                                 >  1:9599505/1‑151 (MQ=255)
                                                  cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCAt                                                                                                 <  1:4692157/151‑1 (MQ=255)
                                                  cGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCAt                                                                                                 <  2:4270025/151‑1 (MQ=255)
                                                   ggTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATg                                                                                                >  2:10555416/1‑151 (MQ=255)
                                                   ggTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATg                                                                                                >  2:5324281/1‑151 (MQ=255)
                                                     tCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAc                                                                                              >  2:5205306/1‑151 (MQ=255)
                                                     tCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAc                                                                                              >  2:9834818/1‑151 (MQ=255)
                                                       aCCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAccc                                                                                            >  1:3579978/1‑151 (MQ=255)
                                                         cGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                          <  2:10711016/151‑1 (MQ=255)
                                                         cGGCCTGACCCATCGGGAGGCGTACAAGGACATGTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                          <  2:6960836/151‑1 (MQ=255)
                                                          ggCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGa                                                                                         >  1:6000662/1‑151 (MQ=255)
                                                          ggCCTGACCCAACGGGAGGCGTACACGCACATTTTCGGCATTACCCTGATCAAGACCCGGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCAGCTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGa                                                                                         <  2:9079554/151‑1 (MQ=255)
                                                           gCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaa                                                                                        >  1:2228328/1‑151 (MQ=255)
                                                            ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaaa                                                                                       <  2:3640313/151‑1 (MQ=255)
                                                            ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaaa                                                                                       <  1:5033010/151‑1 (MQ=255)
                                                            ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaaa                                                                                       >  2:8271553/1‑151 (MQ=255)
                                                             cTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAg                                                                                      <  1:5384892/151‑1 (MQ=255)
                                                             cTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAg                                                                                      >  2:2152285/1‑151 (MQ=255)
                                                               gACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGc                                                                                    >  1:5195674/1‑151 (MQ=255)
                                                                aCCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCa                                                                                   >  1:8641579/1‑151 (MQ=255)
                                                                aCCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCa                                                                                   >  1:10536596/1‑151 (MQ=255)
                                                                aCCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAACCTGAAAGGCa                                                                                   >  1:3092468/1‑151 (MQ=255)
                                                                 cccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCaa                                                                                  >  2:495704/1‑151 (MQ=255)
                                                                 cccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCaa                                                                                  <  2:485808/151‑1 (MQ=255)
                                                                   cATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGa                                                                                >  1:5527576/1‑151 (MQ=255)
                                                                     tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAcc                                                                              <  1:6652977/151‑1 (MQ=255)
                                                                      cGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                             >  2:8098221/1‑151 (MQ=255)
                                                                      cGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                             <  1:107986/151‑1 (MQ=255)
                                                                       gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                            >  2:3367284/1‑151 (MQ=255)
                                                                        ggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGcc                                                                           >  2:1600898/1‑151 (MQ=255)
                                                                           ggCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTg                                                                        >  2:2381905/1‑151 (MQ=255)
                                                                             cGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGt                                                                      <  2:5155484/151‑1 (MQ=255)
                                                                               tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCa                                                                    >  1:9695853/1‑151 (MQ=255)
                                                                               tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCa                                                                    >  2:6529653/1‑151 (MQ=255)
                                                                                 cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACGGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                  >  2:5766816/1‑151 (MQ=255)
                                                                                    ggtcATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                               >  1:10115875/4‑151 (MQ=255)
                                                                                    ggACCTTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                               >  1:8983793/1‑151 (MQ=255)
                                                                                    ggACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                               >  2:10852426/1‑151 (MQ=255)
                                                                                     gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGtt                                                              >  1:1855702/1‑151 (MQ=255)
                                                                                      aCATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTc                                                             <  1:8445159/151‑1 (MQ=255)
                                                                                      aCATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTc                                                             <  1:9361486/151‑1 (MQ=255)
                                                                                      aCATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTc                                                             <  1:4583797/151‑1 (MQ=255)
                                                                                       cATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTcc                                                            <  2:1108174/151‑1 (MQ=255)
                                                                                        aTTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGGTcca                                                           >  2:3030706/1‑151 (MQ=255)
                                                                                         ttttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccac                                                          <  2:7922841/151‑1 (MQ=255)
                                                                                             cGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGc                                                      <  1:10333476/151‑1 (MQ=255)
                                                                                              ggCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                  <  2:4511739/138‑1 (MQ=255)
                                                                                              ggCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                  >  1:4511739/1‑138 (MQ=255)
                                                                                               gCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCgg                                                    >  1:8793964/1‑151 (MQ=255)
                                                                                                   tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                                >  2:10381735/1‑151 (MQ=255)
                                                                                                   tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGGTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                                <  1:2498368/151‑1 (MQ=255)
                                                                                                    aCCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCg                                               >  1:4152605/1‑151 (MQ=255)
                                                                                                       cTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTGTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGcc                                            <  2:6103903/151‑1 (MQ=255)
                                                                                                         gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTg                                          >  1:7174875/1‑151 (MQ=255)
                                                                                                           tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTggg                                        >  1:697120/1‑151 (MQ=255)
                                                                                                           tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTggg                                        >  1:709540/1‑151 (MQ=255)
                                                                                                           tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTggg                                        >  2:2722883/1‑151 (MQ=255)
                                                                                                             aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCa                                      >  2:3609905/1‑151 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                    <  2:8639570/151‑1 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTATTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                    <  1:3711807/151‑1 (MQ=255)
                                                                                                                aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCCGCGGCATCGGCCTGGGCATCg                                   >  2:7390609/1‑151 (MQ=255)
                                                                                                                aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCg                                   <  1:9173970/151‑1 (MQ=255)
                                                                                                                aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCg                                   >  1:483678/1‑151 (MQ=255)
                                                                                                                        ggTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGc                           >  2:6748740/1‑151 (MQ=255)
                                                                                                                         gtgtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGcc                          <  1:1721992/151‑1 (MQ=255)
                                                                                                                          tgtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCt                         >  1:8191150/1‑151 (MQ=255)
                                                                                                                            ttcttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTgg                       >  1:1452050/1‑151 (MQ=255)
                                                                                                                             tcttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGc                      >  1:293502/1‑151 (MQ=255)
                                                                                                                               ttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCg                    <  1:6433463/151‑1 (MQ=255)
                                                                                                                                  gTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAgg                 <  1:2902683/151‑1 (MQ=255)
                                                                                                                                       cGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgc            >  2:6338279/1‑151 (MQ=255)
                                                                                                                                       cGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgc            >  2:7675681/1‑151 (MQ=255)
                                                                                                                                        ggACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgcg           >  2:3979006/1‑151 (MQ=255)
                                                                                                                                           cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg        >  2:323146/1‑151 (MQ=255)
                                                                                                                                           cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg        >  2:1761233/1‑151 (MQ=255)
                                                                                                                                            tGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGa       >  1:2433303/1‑151 (MQ=255)
                                                                                                                                                 tACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTGc  >  2:9188026/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTGC  >  NC_008463/3443144‑3443439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑