Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,384 A→G Q128Q (CAA→CAG)  PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,3840AG99.7% 1482.7 / NA 380Q128Q (CAA→CAG) PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (184/195);  minor base T (1/0);  total (185/195)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

GCCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAG  >  NC_008463/3670236‑3670531
                                                                                                                                                    |                                                                                                                                                   
gCCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGc                                                                                                                                                   >  1:3584175/1‑151 (MQ=255)
 ccccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGACGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGcc                                                                                                                                                  >  2:5559062/1‑151 (MQ=255)
  cccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                 <  1:9361403/151‑1 (MQ=255)
  cccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                 <  1:2105109/151‑1 (MQ=255)
   ccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcg                                                                                                                                                <  1:9644178/151‑1 (MQ=255)
     tACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgcg                                                                                                                                              >  1:3391666/1‑151 (MQ=255)
     tACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgcg                                                                                                                                              >  2:7794764/1‑151 (MQ=255)
       cATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGAc                                                                                                                                            <  2:4163572/151‑1 (MQ=255)
         tCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGt                                                                                                                                          <  1:1717867/151‑1 (MQ=255)
         tCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGt                                                                                                                                          >  2:7561714/1‑151 (MQ=255)
          cAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTg                                                                                                                                         >  1:23077/1‑151 (MQ=255)
          cAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTg                                                                                                                                         <  1:2892680/151‑1 (MQ=255)
            gCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCt                                                                                                                                       >  1:4419661/1‑151 (MQ=255)
             ccAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                      >  1:1375865/1‑151 (MQ=255)
             ccAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                      <  1:6529681/151‑1 (MQ=255)
             ccAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTc                                                                                                                                      <  2:8549616/151‑1 (MQ=255)
               aaCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGc                                                                                                                                    >  1:7226276/1‑151 (MQ=255)
                  tGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                 <  1:7482050/151‑1 (MQ=255)
                  tGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                 <  1:10244832/151‑1 (MQ=255)
                   gATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                                >  2:5805134/1‑151 (MQ=255)
                   gATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                                <  1:4495747/151‑1 (MQ=255)
                     tCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGt                                                                                                                              <  1:5615730/151‑1 (MQ=255)
                     tCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGt                                                                                                                              >  2:9373071/1‑151 (MQ=255)
                         cGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAtt                                                                                                                          <  1:2245075/151‑1 (MQ=255)
                          gagaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAttt                                                                                                                         <  1:4116636/151‑1 (MQ=255)
                            gaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                       <  2:3157546/151‑1 (MQ=255)
                            gaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                       >  1:9503078/1‑151 (MQ=255)
                             aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACg                                                                                                                      <  1:7059458/151‑1 (MQ=255)
                               ggACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGc                                                                                                                    <  1:3141593/151‑1 (MQ=255)
                                gACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCt                                                                                                                   >  1:8733402/1‑151 (MQ=255)
                                 aCGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCtt                                                                                                                  >  1:5726374/1‑151 (MQ=255)
                                    gCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcg                                                                                                               <  1:2709145/151‑1 (MQ=255)
                                     cTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcgc                                                                                                              <  1:3233537/151‑1 (MQ=255)
                                         gTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                          <  2:10028602/151‑1 (MQ=255)
                                              ttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATc                                                                                                     <  1:2952393/151‑1 (MQ=255)
                                               tGGCCGCCGCTCCCCTACCGCTACGACACCATCAACTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATcc                                                                                                    <  2:8840910/151‑1 (MQ=255)
                                               tGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATcc                                                                                                    <  1:9189886/151‑1 (MQ=255)
                                                    gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTc                                                                                               >  1:4007758/1‑151 (MQ=255)
                                                    gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTc                                                                                               <  1:7791724/151‑1 (MQ=255)
                                                     ccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCg                                                                                              <  1:6421563/151‑1 (MQ=255)
                                                       gATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGcc                                                                                            >  2:9022782/1‑151 (MQ=255)
                                                         tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                          <  1:6576981/151‑1 (MQ=255)
                                                             ctccAGCTACGACACCATCACCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                      <  1:3737472/148‑1 (MQ=255)
                                                             cTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                      <  1:11035292/151‑1 (MQ=255)
                                                              tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                     >  1:10492499/1‑151 (MQ=255)
                                                              tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                     >  2:3408596/1‑151 (MQ=255)
                                                                 aGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctga                                                                                  >  2:1974347/1‑151 (MQ=255)
                                                                 aGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGACGGTCCTCTTCGccctgaccctga                                                                                  >  2:8107288/1‑151 (MQ=255)
                                                                   cTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                                >  1:10010721/1‑151 (MQ=255)
                                                                   cTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCctgaccctgacc                                                                                >  2:7073985/1‑151 (MQ=255)
                                                                    tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCg                                                                               <  1:8221547/151‑1 (MQ=255)
                                                                     aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                              <  1:4555966/151‑1 (MQ=255)
                                                                     aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                              >  2:5359138/1‑151 (MQ=255)
                                                                     aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                              <  1:3838414/151‑1 (MQ=255)
                                                                          acCATCAACTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCa                                                                         <  2:8646483/151‑1 (MQ=255)
                                                                             aTCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGtt                                                                      >  2:3790990/1‑151 (MQ=255)
                                                                                aaCTCCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGCCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTACGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                   <  2:1452860/151‑1 (MQ=255)
                                                                                aaCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                   <  1:9703465/151‑1 (MQ=255)
                                                                                  cTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTg                                                                 >  2:504596/1‑151 (MQ=255)
                                                                                      gAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCCCCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                             >  1:2367461/1‑151 (MQ=255)
                                                                                      gAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                             <  2:6290085/151‑1 (MQ=255)
                                                                                        gCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGc                                                           <  1:1988425/151‑1 (MQ=255)
                                                                                         cTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCg                                                          >  1:5451079/1‑151 (MQ=255)
                                                                                          tGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                         >  2:3356679/1‑151 (MQ=255)
                                                                                                  cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCg                                                 >  2:10618937/1‑151 (MQ=255)
                                                                                                   ccgcccccgcccCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTAACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCgg                                                >  2:4455885/1‑151 (MQ=255)
                                                                                                     gcccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTACGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcg                                              >  2:7243907/1‑151 (MQ=255)
                                                                                                        cccgccccgcCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGccc                                           <  2:669877/151‑1 (MQ=255)
                                                                                                          cgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTg                                         <  1:8346309/151‑1 (MQ=255)
                                                                                                           gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                        >  2:248991/1‑151 (MQ=255)
                                                                                                           gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                        >  2:1638423/1‑151 (MQ=255)
                                                                                                            ccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCa                                       <  2:10792253/151‑1 (MQ=255)
                                                                                                            ccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCa                                       <  2:10267527/151‑1 (MQ=255)
                                                                                                             cccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCaa                                      <  2:2217545/151‑1 (MQ=255)
                                                                                                                gccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGc                                   <  2:8182002/151‑1 (MQ=255)
                                                                                                                gccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGc                                   <  2:4782215/151‑1 (MQ=255)
                                                                                                                 ccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGct                                  <  2:3609143/151‑1 (MQ=255)
                                                                                                                 ccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGct                                  >  1:669286/1‑151 (MQ=255)
                                                                                                                  cgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGAGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCTATCCTCTTCGCCCTGAACCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGcta                                 >  2:5669146/1‑151 (MQ=255)
                                                                                                                     cggcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctacta                              >  1:5996704/1‑151 (MQ=255)
                                                                                                                      ggcggACAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCtactac                             <  2:2702657/151‑1 (MQ=255)
                                                                                                                      ggcggAAAACTGGCTGGGCACCGACGACCATGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCtactac                             >  2:2324314/1‑151 (MQ=255)
                                                                                                                       gcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTACGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCTGCGTCACCGTCGGCGCCCAGCAAGGCTACTAcg                            >  2:4525612/1‑151 (MQ=255)
                                                                                                                        cggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcgg                           <  1:1614865/151‑1 (MQ=255)
                                                                                                                         ggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggc                          <  2:9711878/151‑1 (MQ=255)
                                                                                                                         ggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAATTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggc                          >  1:5078536/1‑151 (MQ=255)
                                                                                                                           aaaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcgg                        >  2:131879/1‑151 (MQ=255)
                                                                                                                            aaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCGGACCGTGCTCAGTTCTCTGATCGGCGTCAGCGTCGGCGCCCTGCAAGGCTACTACggcggc                       <  1:8056893/151‑1 (MQ=255)
                                                                                                                                tggctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTggg                   >  1:6209068/1‑151 (MQ=255)
                                                                                                                                    tggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGa               <  2:2130066/151‑1 (MQ=255)
                                                                                                                                    tggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGa               >  2:187704/1‑151 (MQ=255)
                                                                                                                                      ggCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGAcc             <  2:5888756/151‑1 (MQ=255)
                                                                                                                                       gCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCt            <  2:6206153/151‑1 (MQ=255)
                                                                                                                                       gCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCt            <  1:8929935/151‑1 (MQ=255)
                                                                                                                                        cACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTg           <  1:2581388/151‑1 (MQ=255)
                                                                                                                                         aCCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGc          >  1:7407738/1‑151 (MQ=255)
                                                                                                                                         aCCGACGACCAGGCCCGCGACGTGCTCGCCCGGGGGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGc          >  1:10768238/1‑151 (MQ=255)
                                                                                                                                           cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTc        <  2:8714181/151‑1 (MQ=255)
                                                                                                                                           cgacgaCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTc        <  1:4714816/151‑1 (MQ=255)
                                                                                                                                            gacgacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCg       >  2:10342225/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAG  >  NC_008463/3670236‑3670531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑