Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,933,352 C→G P346P (CCC→CCG)  PA14_RS17955 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,933,3520CG99.8% 1937.7 / NA 494P346P (CCC→CCG) PA14_RS17955MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (227/266);  minor base T (0/1);  total (227/267)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 506 total aligned reads displayed.

CGATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGTC  >  NC_008463/3933204‑3933499
                                                                                                                                                    |                                                                                                                                                   
 gATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCggcg                                                                                                                                                  >  2:3476993/1‑151 (MQ=255)
 gATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCggcg                                                                                                                                                  >  2:6780409/1‑151 (MQ=255)
     gTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGa                                                                                                                                              >  1:5772271/1‑151 (MQ=255)
      ttCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGac                                                                                                                                             >  2:7803129/1‑151 (MQ=255)
       tCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGTCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGacc                                                                                                                                            <  1:2538770/151‑1 (MQ=255)
         ccACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGaccac                                                                                                                                          >  1:4690257/1‑151 (MQ=255)
             cGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgc                                                                                                                                      >  2:2385648/1‑151 (MQ=255)
              gCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgca                                                                                                                                     <  2:10383485/151‑1 (MQ=255)
               ccTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgcag                                                                                                                                    <  1:8918500/151‑1 (MQ=255)
                cTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgcagc                                                                                                                                   <  1:8431560/151‑1 (MQ=255)
                cTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGgcagc                                                                                                                                   <  1:2266897/151‑1 (MQ=255)
                  tCGAGGTAGCCGGCCAGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCAt                                                                                                                                 <  1:1170140/151‑1 (MQ=255)
                    gAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTc                                                                                                                               <  1:4964575/151‑1 (MQ=255)
                    gAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTc                                                                                                                               >  1:2680488/1‑151 (MQ=255)
                    gAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTc                                                                                                                               <  1:2741432/151‑1 (MQ=255)
                      ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCg                                                                                                                             >  1:9086805/1‑151 (MQ=255)
                      ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCg                                                                                                                             <  2:10185382/151‑1 (MQ=255)
                      ggTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCAGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCg                                                                                                                             <  1:1330087/151‑1 (MQ=255)
                          gCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGagag                                                                                                                         >  1:7003681/1‑151 (MQ=255)
                           ccGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGt                                                                                                                        <  1:10731606/151‑1 (MQ=255)
                            cGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTg                                                                                                                       <  2:6627444/151‑1 (MQ=255)
                             ggCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTgg                                                                                                                      >  2:6708882/1‑151 (MQ=255)
                              gCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                     <  2:6031925/151‑1 (MQ=255)
                              gCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCGGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGt                                                                                                                     <  2:7630894/151‑1 (MQ=255)
                               ccTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTc                                                                                                                    <  1:2955879/151‑1 (MQ=255)
                                cTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCg                                                                                                                   <  1:8143767/151‑1 (MQ=255)
                                 tGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                  <  1:11047788/151‑1 (MQ=255)
                                 tGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCgg                                                                                                                  >  1:10420482/1‑151 (MQ=255)
                                  gCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGc                                                                                                                 <  2:7341203/151‑1 (MQ=255)
                                   cTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGcc                                                                                                                >  2:10847903/1‑151 (MQ=255)
                                       gCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCctgct                                                                                                            <  1:9254348/151‑1 (MQ=255)
                                           gATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGTTCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTc                                                                                                        >  2:3733191/1‑151 (MQ=255)
                                           gATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTc                                                                                                        <  1:10602399/151‑1 (MQ=255)
                                             tCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCat                                                                                                      <  2:4740186/151‑1 (MQ=255)
                                              ccTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCatg                                                                                                     <  1:3524757/151‑1 (MQ=255)
                                                   tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATggg                                                                                                <  2:9142424/151‑1 (MQ=255)
                                                   tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATggg                                                                                                <  1:5662368/151‑1 (MQ=255)
                                                   tCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATggg                                                                                                <  2:177120/151‑1 (MQ=255)
                                                    cGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGa                                                                                               >  2:5549885/1‑151 (MQ=255)
                                                     ggCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGAc                                                                                              <  1:4318110/151‑1 (MQ=255)
                                                     ggCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGAc                                                                                              >  1:826489/1‑151 (MQ=255)
                                                           gCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGAccc                                                                                        >  1:5060777/1‑151 (MQ=255)
                                                           gCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGAccc                                                                                        >  2:2838333/1‑151 (MQ=255)
                                                            ccTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCt                                                                                       >  1:3268240/1‑151 (MQ=255)
                                                             cTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTg                                                                                      >  2:2665701/1‑151 (MQ=255)
                                                              tCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCCTTCCGATAGTGGTCGGCCTGCTGTGCATGATGGGACTGACCCTGt                                                                                     >  2:7173231/1‑151 (MQ=255)
                                                              tCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGt                                                                                     <  2:793167/151‑1 (MQ=255)
                                                                ggACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTAc                                                                                   <  2:3351513/151‑1 (MQ=255)
                                                                 gACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACg                                                                                  <  1:6260100/151‑1 (MQ=255)
                                                                  aCAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACgg                                                                                 >  1:10512337/1‑151 (MQ=255)
                                                                   cAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGc                                                                                <  2:5230189/151‑1 (MQ=255)
                                                                   cAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGc                                                                                <  2:250240/151‑1 (MQ=255)
                                                                     aGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGccc                                                                              <  2:1949981/151‑1 (MQ=255)
                                                                       cTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCgg                                                                            <  1:8592458/151‑1 (MQ=255)
                                                                       cTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCgg                                                                            <  2:4490440/151‑1 (MQ=255)
                                                                        tGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGa                                                                           <  2:8310429/151‑1 (MQ=255)
                                                                        tGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGa                                                                           <  2:5823890/151‑1 (MQ=255)
                                                                         gTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGAc                                                                          <  1:6017505/151‑1 (MQ=255)
                                                                          ttCGGCGCACGGCGCATGCCGGCCAGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACt                                                                         <  1:4496680/151‑1 (MQ=255)
                                                                           tCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTc                                                                        <  2:9591087/151‑1 (MQ=255)
                                                                           tCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTc                                                                        >  2:9898705/1‑151 (MQ=255)
                                                                            cggcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCg                                                                       >  2:10054173/1‑151 (MQ=255)
                                                                             ggcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCga                                                                      >  2:8561081/1‑151 (MQ=255)
                                                                              gcgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCgat                                                                     <  2:1097326/151‑1 (MQ=255)
                                                                               cgcacggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCgatg                                                                    >  1:7723503/1‑151 (MQ=255)
                                                                                  acggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATc                                                                 <  1:1972964/151‑1 (MQ=255)
                                                                                   cggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCt                                                                <  2:8084587/151‑1 (MQ=255)
                                                                                    ggcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTg                                                               <  1:6384884/151‑1 (MQ=255)
                                                                                     gcgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGc                                                              <  1:2070348/151‑1 (MQ=255)
                                                                                      cgcaTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGCTGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCg                                                             >  2:6184731/1‑151 (MQ=255)
                                                                                        caTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGTTGATCTGCGGc                                                           >  2:5780719/1‑151 (MQ=255)
                                                                                         aTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                          <  2:2083059/151‑1 (MQ=255)
                                                                                         aTGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGAATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCt                                                          <  2:4920688/151‑1 (MQ=255)
                                                                                                 cTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCGGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCgccgcc                                                  >  1:4157113/1‑151 (MQ=255)
                                                                                                   gCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCAt                                                >  1:9477854/1‑151 (MQ=255)
                                                                                                    cGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGTCTCGATGATATGCGGCTCCGCCGCCATc                                               <  2:1272312/151‑1 (MQ=255)
                                                                                                    cGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATc                                               >  2:1548866/1‑151 (MQ=255)
                                                                                                    cGTGATCAGCCAGCGCGCGCTGACCGTCTGCCTGGCGCGGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATCGGGGTCGGCCAGCTGTTCATGATGGGACTGCCCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATc                                               <  2:6973819/151‑1 (MQ=255)
                                                                                                      tGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGa                                             >  1:6599929/1‑151 (MQ=255)
                                                                                                          cAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTc                                         <  2:3986905/151‑1 (MQ=255)
                                                                                                             ccTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGc                                      >  2:8861161/1‑151 (MQ=255)
                                                                                                               tgctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCAc                                    >  1:2876650/1‑151 (MQ=255)
                                                                                                                 ctgGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCg                                  <  1:7283475/151‑1 (MQ=255)
                                                                                                                   ggctcagACCGGCTGCCTGGCGCTGATCGTCCCGGCGATGACCTCCGGCAGCATTCCGATCGTGGTCGGCCTGCTGTTCAAGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGc                                <  2:9438961/145‑1 (MQ=21)
                                                                                                                     cgcTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGa                              >  1:9185427/1‑151 (MQ=255)
                                                                                                                       cTGACCGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAgg                            >  2:6456554/1‑151 (MQ=255)
                                                                                                                            cGTCTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGgccgcc                       <  1:4382665/151‑1 (MQ=255)
                                                                                                                               cTGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGc                    >  1:10005680/1‑151 (MQ=255)
                                                                                                                                tGCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCa                   >  2:8220442/1‑151 (MQ=255)
                                                                                                                                 gCCTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCAc                  <  1:5317422/151‑1 (MQ=255)
                                                                                                                                  ccTGGCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCAcc                 <  1:5486476/151‑1 (MQ=255)
                                                                                                                                     ggCGCTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGcc              >  1:1595162/1‑151 (MQ=255)
                                                                                                                                      gcgcTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCt             <  1:10181165/151‑1 (MQ=255)
                                                                                                                                         cTGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCg          >  1:7114968/1‑151 (MQ=255)
                                                                                                                                          tGTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCgg         >  1:3801897/1‑151 (MQ=255)
                                                                                                                                           gTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGc        >  2:3131066/1‑151 (MQ=255)
                                                                                                                                           gTTCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGc        >  2:9235989/1‑151 (MQ=255)
                                                                                                                                            ttCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCt       >  1:4048748/1‑151 (MQ=255)
                                                                                                                                            ttCGTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACACGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCt       >  2:4220612/1‑151 (MQ=255)
                                                                                                                                               gTCCCGGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCg    >  1:9764915/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGATCGTTCCCACCGCCTTCGAGGTAGCCGGCCTGCTCGGCCCGATCCTGATCGGCTTCGCCTCGGACAAGCTGTTCGGCGCACGGCGCATGCCGGCCTGCGTGATCAGCCTGCTGGCGCTGACCGTCTGCCTGGCGCTGTTCGTCCCCGCGATGACCACCGGCAGCATTCCGATAGTGGTCGGCCTGCTGTTCATGATGGGACTGACCCTGTACGGCCCGGACTCGATGATCTGCGGCTCCGCCGCCATCGACTTCGGCACCGGCGAGGCCGCCGGCACCGCCTCCGGCTTCGTC  >  NC_008463/3933204‑3933499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑