Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 208,430 T→G intergenic (‑36/+52) atsA ← / ← PA14_RS00940 arylsulfatase AtsA/ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463208,4300TG99.8% 2231.4 / NA 570intergenic (‑36/+52)atsA/PA14_RS00940arylsulfatase AtsA/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (259/310);  minor base C (1/0);  total (260/310)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 591 total aligned reads displayed.

GCGATGGCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGTCCGTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTGCAGCAGCGCTTC  >  NC_008463/208282‑208577
                                                                                                                                                    |                                                                                                                                                   
gCGATGGCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGcc                                                                                                                                                   <  1:5146280/151‑1 (MQ=255)
 cGATGGCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGccc                                                                                                                                                  >  2:1318656/1‑150 (MQ=255)
  gATGGCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGccct                                                                                                                                                 <  2:4607371/151‑3 (MQ=255)
     ggCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTAGGGGCGTTTGCTCATGCGCGGCTCTCGACTGGATGGGAGGAGAGGGTGGGGCCCTCGc                                                                                                                                              >  2:7548664/1‑151 (MQ=255)
       ccAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGccc                                                                                                                                            >  1:1348109/1‑151 (MQ=255)
        cAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGcccc                                                                                                                                           >  2:1879002/1‑151 (MQ=255)
        cAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGcccc                                                                                                                                           >  1:117758/1‑151 (MQ=255)
         aGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGccccc                                                                                                                                          >  1:6553236/1‑151 (MQ=255)
         aGGGCGTCGAGGGTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGCTATCGGAGTAGCACAGGTCGTCGGCGACGATCACCCGCACGTGGTGGCGTGTCCTCATGCGCGCGTCTCGGCGGGAGGGGAGGAGAGGGTGGGGCCCTCGccccc                                                                                                                                          >  2:9841922/1‑151 (MQ=255)
              gTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCt                                                                                                                                     >  1:1441942/1‑151 (MQ=255)
              gTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGACGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCt                                                                                                                                     <  2:4753195/151‑1 (MQ=255)
               tCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCtt                                                                                                                                    >  2:2177947/1‑151 (MQ=255)
                cGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTc                                                                                                                                   <  2:10468115/151‑1 (MQ=255)
                 gAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGACGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGCGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTcc                                                                                                                                  >  2:7003847/1‑151 (MQ=255)
                    gTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGc                                                                                                                               <  2:3870482/151‑1 (MQ=255)
                       cGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCCGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAgg                                                                                                                            >  1:8231062/1‑151 (MQ=255)
                        ggCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAggg                                                                                                                           >  1:6648649/1‑151 (MQ=255)
                         gCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAgggg                                                                                                                          <  1:10242336/151‑1 (MQ=255)
                          cGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAggggg                                                                                                                         <  2:7723082/151‑1 (MQ=255)
                            tGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGaa                                                                                                                       <  2:2896261/151‑1 (MQ=255)
                               cGATCTCGCCGCCGAAGGCGCCGATATCGGAGACGCCCAGGTCGTCGGCGACGATCACCAGGAATTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAgg                                                                                                                    <  2:5506042/151‑1 (MQ=255)
                               cGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAgg                                                                                                                    >  1:3755528/1‑151 (MQ=255)
                                gATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAggg                                                                                                                   >  2:7966442/1‑151 (MQ=255)
                                 aTCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGc                                                                                                                  >  1:4106534/1‑151 (MQ=255)
                                  tctcGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGcc                                                                                                                 <  1:11035713/151‑1 (MQ=255)
                                   ctcGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCa                                                                                                                <  2:4088716/151‑1 (MQ=255)
                                    tcGCCGCCGAAGGCGCCGATATCGGAGAAGCCCTGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAg                                                                                                               <  2:7078891/151‑1 (MQ=255)
                                    tcGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAg                                                                                                               <  1:3450966/151‑1 (MQ=255)
                                     cgccgcCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAgg                                                                                                              >  2:784568/1‑151 (MQ=255)
                                     cgccgcCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAgg                                                                                                              <  2:8308372/151‑1 (MQ=255)
                                      gccgccGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAggg                                                                                                             >  1:3014274/1‑151 (MQ=255)
                                      gccgccGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAggg                                                                                                             <  2:3388008/151‑1 (MQ=255)
                                      gccgccGAAGGCGCCGAGATCGGAGAAGCCCAGGTCGTCGGCGACGATCCCCAGGAAGTTGGCGCGTGTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGACAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAggg                                                                                                             <  2:8999896/151‑1 (MQ=255)
                                       ccgccgAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGc                                                                                                            <  2:6649005/151‑1 (MQ=255)
                                          ccgAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAg                                                                                                         >  2:9360303/1‑151 (MQ=255)
                                          ccgAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAg                                                                                                         <  2:8455321/151‑1 (MQ=255)
                                              aGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGc                                                                                                     >  2:5942185/1‑151 (MQ=255)
                                              aGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGCGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGc                                                                                                     >  1:9019578/1‑151 (MQ=255)
                                                   ccGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTAGCTCATGCGCGGGTCTCGTCTGGCTGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTc                                                                                                <  1:5279267/151‑1 (MQ=255)
                                                     gATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTGTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCGTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAg                                                                                              <  2:10274930/151‑1 (MQ=255)
                                                       tatCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCa                                                                                            >  1:228071/1‑151 (MQ=255)
                                                       tatCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCa                                                                                            <  1:6834636/151‑1 (MQ=255)
                                                           ggAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCCTGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCg                                                                                        <  1:9583617/151‑1 (MQ=255)
                                                                  cccAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAAtt                                                                                 <  1:3232354/151‑1 (MQ=255)
                                                                     aGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGa                                                                              >  2:5168305/1‑151 (MQ=255)
                                                                       gtcgtcGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGatg                                                                            >  1:8034223/1‑149 (MQ=255)
                                                                        tcgtcgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAgg                                                                           <  2:8123268/151‑1 (MQ=255)
                                                                         cgtcgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAggg                                                                          <  1:4025691/151‑1 (MQ=255)
                                                                          gtcgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGc                                                                         <  2:1653551/151‑1 (MQ=255)
                                                                           tcgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCa                                                                        <  2:9628220/151‑1 (MQ=255)
                                                                            cgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAg                                                                       <  1:2493458/151‑1 (MQ=255)
                                                                            cgGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAg                                                                       >  1:10835016/1‑151 (MQ=255)
                                                                              gCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGt                                                                     <  2:7633626/151‑1 (MQ=255)
                                                                              gCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGt                                                                     <  2:10077379/151‑1 (MQ=255)
                                                                               cgccgaTCACCAGGCAGATGGGGCGTTTGCTCATGCGCGGGGCTCGTCGGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGtt                                                                    <  1:881111/148‑1 (MQ=255)
                                                                                gacgaTCACGAGGTAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCGGGATGGGAGGTGTGGGTGGGGCCCTCGCGCCCGCCCTTCCCGCTGGGGGTCAGGGCGAGGGCAAGGGGCGTTTCAGGAAGCGAGGAATTCGTCGGGCGGGttg                                                                   >  2:8748859/1‑150 (MQ=255)
                                                                                gacgaTCACCAGGAAGTTGGGGCGTGTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGttt                                                                   <  2:5704205/151‑1 (MQ=255)
                                                                                 acgaTCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGtttt                                                                  >  2:10743802/1‑151 (MQ=255)
                                                                                   gaTCACCTGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGTTGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCGTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTcc                                                                <  1:9947063/151‑1 (MQ=255)
                                                                                    aTCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCa                                                               <  2:8919459/151‑1 (MQ=255)
                                                                                    aTCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCa                                                               <  1:5789812/151‑1 (MQ=255)
                                                                                      cACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCAcc                                                             <  2:1459345/151‑1 (MQ=255)
                                                                                      cACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCAcc                                                             <  2:2323070/151‑1 (MQ=255)
                                                                                      cACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCAcc                                                             <  1:6320100/151‑1 (MQ=255)
                                                                                       aCCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCAccc                                                            <  2:10165213/151‑1 (MQ=255)
                                                                                        ccAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCg                                                           <  1:4412020/151‑1 (MQ=255)
                                                                                         cAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGt                                                          <  1:6462260/151‑1 (MQ=255)
                                                                                         cAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGt                                                          >  1:6063816/1‑151 (MQ=255)
                                                                                             aaGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTcgcg                                                      <  1:9376197/151‑1 (MQ=255)
                                                                                               gTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCgcggc                                                    <  1:10594144/151‑1 (MQ=255)
                                                                                               gTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCgcggc                                                    >  1:2393172/1‑151 (MQ=255)
                                                                                                 tGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCgcggcgg                                                  >  1:9722258/1‑151 (MQ=255)
                                                                                                    ggCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCgcggcggcgg                                               >  1:9170432/1‑151 (MQ=255)
                                                                                                      cGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCgcggcggcggcg                                             >  1:4809206/1‑151 (MQ=255)
                                                                                                       gTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGgcgc                                            >  2:6327226/1‑151 (MQ=255)
                                                                                                       gTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGgcgc                                            <  1:7144210/151‑1 (MQ=255)
                                                                                                        tttGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGcgcg                                           <  1:7939366/151‑1 (MQ=255)
                                                                                                          tGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGAc                                         >  2:9207812/1‑151 (MQ=255)
                                                                                                               aTGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGACTTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACt                                    >  1:7454262/1‑151 (MQ=255)
                                                                                                               aTGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACt                                    <  2:10400737/151‑1 (MQ=255)
                                                                                                                   gcgGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGCAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGcc                                <  1:4718943/151‑1 (MQ=255)
                                                                                                                   gcgGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGcc                                <  2:4960437/151‑1 (MQ=255)
                                                                                                                    cgGGTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGGTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCg                               >  1:1953072/1‑151 (MQ=255)
                                                                                                                     gggTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGt                              >  2:7369477/1‑151 (MQ=255)
                                                                                                                     gggTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGt                              <  1:3892685/151‑1 (MQ=255)
                                                                                                                       gTCTCGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcg                            >  1:2080227/1‑151 (MQ=255)
                                                                                                                        tctcGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcgg                           <  1:4584419/151‑1 (MQ=255)
                                                                                                                        tctcGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcgg                           <  1:294152/151‑1 (MQ=255)
                                                                                                                        tctcGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCGTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcgg                           <  2:3212048/151‑1 (MQ=255)
                                                                                                                          tcGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcggcg                         <  1:8401985/151‑1 (MQ=255)
                                                                                                                          tcGTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcggcg                         <  2:3585440/151‑1 (MQ=255)
                                                                                                                            gTCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCCCCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTcggcggg                       >  2:4356211/1‑150 (MQ=255)
                                                                                                                             tCTGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTc                      <  1:2758039/151‑1 (MQ=255)
                                                                                                                               tGGATGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAg                    >  2:11046372/1‑151 (MQ=255)
                                                                                                                                   tGGGAGGAGAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCg                <  1:10163656/151‑1 (MQ=255)
                                                                                                                                       aggagAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTgca            >  1:756915/1‑151 (MQ=255)
                                                                                                                                        ggagAGGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTgcag           <  2:2496791/151‑1 (MQ=255)
                                                                                                                                            agGGTGGGGCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTGCagcagc       >  2:9087588/1‑151 (MQ=255)
                                                                                                                                             gggtggggCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTGCAGCAgcg      <  1:10044639/151‑1 (MQ=255)
                                                                                                                                                tggggCCCTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTGCAGCAGCGCtt   >  2:6045950/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGATGGCCAGGGCGTCGAGGTTCGGCGTGGCGATCTCGCCGCCGAAGGCGCCGATATCGGAGAAGCCCAGGTCGTCGGCGACGATCACCAGGAAGTTGGGGCGTTTGCTCATGCGCGGGTCTCGTCTGGATGGGAGGAGAGGGTGGGTCCGTCGCCCCCGCCCTTCCCGCAGGGGGAAAGGGCCAGGGCAAGGGGCTTTTCAGCAAGCGAGGAATTCGAAGGGCAGGTTTTCCACCCGTCGCGGCGGCGGCGCGACGTACTCGCCGTCGGCGGTCAGTTCGTGCAGCAGCGCTTC  >  NC_008463/208282‑208577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑