Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,658 G→C A165A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,6580GC99.9% 2787.1 / NA 713A165A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (302/410);  minor base A (1/0);  total (303/410)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 714 total aligned reads displayed.

CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805
                                                                                                                                                    |                                                                                                                                                   
cccAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGa                                                                                                                                                   <  2:3222649/151‑1 (MQ=255)
 ccAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGaa                                                                                                                                                  >  2:7597735/1‑151 (MQ=255)
  cAGGCCGCCAGGTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAg                                                                                                                                                 <  2:8820727/151‑1 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                <  2:7265683/151‑1 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                >  2:4254877/1‑151 (MQ=255)
   aGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGc                                                                                                                                                <  2:8282670/151‑1 (MQ=255)
     gccgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGa                                                                                                                                              <  2:9458915/151‑1 (MQ=255)
       cgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATc                                                                                                                                            <  2:2758829/151‑1 (MQ=255)
       cgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATc                                                                                                                                            <  2:4091144/151‑1 (MQ=255)
              tGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCgg                                                                                                                                     <  2:11072575/151‑1 (MQ=255)
              tGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCgg                                                                                                                                     >  2:3055076/1‑151 (MQ=255)
                cGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTg                                                                                                                                   >  1:9905487/1‑151 (MQ=255)
                cGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTg                                                                                                                                   <  1:3742727/151‑1 (MQ=255)
                 ggggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgc                                                                                                                                  >  2:6658992/1‑151 (MQ=255)
                    gTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcggc                                                                                                                               <  2:11001421/151‑1 (MQ=255)
                          tACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagg                                                                                                                         <  1:8479969/151‑1 (MQ=255)
                          tACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagg                                                                                                                         >  1:1431983/1‑151 (MQ=255)
                          tACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgagg                                                                                                                         >  2:10521918/1‑151 (MQ=255)
                            cAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgaggag                                                                                                                       >  1:2765238/1‑151 (MQ=255)
                             cgCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCAGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGc                                                                                                                      <  2:5339095/150‑1 (MQ=255)
                              gCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGcc                                                                                                                     >  2:8420629/1‑150 (MQ=255)
                                  cGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGATCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGc                                                                                                                 >  2:6763732/1‑151 (MQ=255)
                                      tGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAgg                                                                                                             <  2:4555213/151‑1 (MQ=255)
                                        ggACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCCAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGgcg                                                                                                           >  2:3810242/1‑151 (MQ=255)
                                        ggACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGgcg                                                                                                           <  1:2052923/151‑1 (MQ=255)
                                         gACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGgcgc                                                                                                          <  1:5485356/151‑1 (MQ=255)
                                          aCGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCt                                                                                                         <  2:5970892/151‑1 (MQ=255)
                                            gCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTgg                                                                                                       >  2:6366951/1‑151 (MQ=255)
                                             cccTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGa                                                                                                      >  1:7797272/1‑151 (MQ=255)
                                                 gCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAgcg                                                                                                  >  2:436390/1‑151 (MQ=255)
                                                  cAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAgcgc                                                                                                 >  2:2881824/1‑151 (MQ=255)
                                                   aaCGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCa                                                                                                >  2:2822607/1‑151 (MQ=255)
                                                   aaCGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCa                                                                                                <  1:3909035/151‑1 (MQ=255)
                                                     cGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAgg                                                                                              <  2:8037562/151‑1 (MQ=255)
                                                      gCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAggg                                                                                             <  2:7406038/151‑1 (MQ=255)
                                                       cAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCTGGCGCTGGAAGCGCAGGGc                                                                                            >  2:2141834/1‑151 (MQ=255)
                                                       cAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGc                                                                                            <  2:2495867/151‑1 (MQ=255)
                                                        aGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCa                                                                                           <  1:3556389/151‑1 (MQ=255)
                                                         gCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCACCGCCAGGCGCTGGAAGCGCAGGGCAt                                                                                          >  2:8993284/1‑151 (MQ=255)
                                                          ccAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCGGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATc                                                                                         <  1:6649772/151‑1 (MQ=255)
                                                             ggTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgcc                                                                                      <  1:2572615/151‑1 (MQ=255)
                                                              gTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgccg                                                                                     <  2:6556633/151‑1 (MQ=255)
                                                               tCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgccgc                                                                                    >  2:8456684/1‑151 (MQ=255)
                                                                  aGGCCGGCACCCGGCTCCTGGTAATCGGCGCCACCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgct                                                                                 <  2:916467/151‑1 (MQ=255)
                                                                   ggCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctg                                                                                <  2:2413363/151‑1 (MQ=255)
                                                                   ggCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctg                                                                                <  1:5287708/151‑1 (MQ=255)
                                                                    gCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctgc                                                                               >  1:2971285/1‑151 (MQ=255)
                                                                    gCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctgc                                                                               >  2:10080990/1‑151 (MQ=255)
                                                                        gCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctgctgct                                                                           >  1:2375224/1‑151 (MQ=255)
                                                                          aCCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTgg                                                                         >  1:9423937/1‑151 (MQ=255)
                                                                           cccGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTggg                                                                        >  2:3649048/1‑151 (MQ=255)
                                                                              ggCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     >  2:10731402/1‑151 (MQ=255)
                                                                              ggCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     <  1:1749848/151‑1 (MQ=255)
                                                                                 tCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgcc                                                                  >  2:3360919/1‑151 (MQ=255)
                                                                                 tCCTGGAAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTTCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgcc                                                                  >  1:516487/1‑151 (MQ=255)
                                                                                     ggTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggcc                                                              <  1:8954757/151‑1 (MQ=255)
                                                                                     ggTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggcc                                                              <  1:3396286/151‑1 (MQ=255)
                                                                                        aaTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGaa                                                           >  1:10322107/1‑151 (MQ=255)
                                                                                        aaTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGaa                                                           <  2:1025997/151‑1 (MQ=255)
                                                                                        aaTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGaa                                                           >  1:9248639/1‑151 (MQ=255)
                                                                                          tCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACt                                                         >  1:10032327/1‑151 (MQ=255)
                                                                                            ggCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTgg                                                       >  2:3485730/1‑151 (MQ=255)
                                                                                              cgcCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTggcg                                                     <  1:10942307/151‑1 (MQ=255)
                                                                                                 cAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGca                                                  >  1:8622213/1‑151 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             <  2:6458830/151‑1 (MQ=255)
                                                                                                       cgcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTg                                            >  1:4852285/1‑151 (MQ=255)
                                                                                                        gcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTgg                                           >  2:2959784/1‑151 (MQ=255)
                                                                                                               gCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGAt                                    <  1:4549813/151‑1 (MQ=255)
                                                                                                               gCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGAt                                    <  2:6222457/151‑1 (MQ=255)
                                                                                                               gCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGAt                                    <  1:6392176/151‑1 (MQ=255)
                                                                                                                cAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATc                                   <  2:10400374/151‑1 (MQ=255)
                                                                                                                 aaGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCt                                  <  2:1577600/151‑1 (MQ=255)
                                                                                                                 aaGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCt                                  <  1:10688458/151‑1 (MQ=255)
                                                                                                                 aaGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCt                                  >  2:8133235/1‑151 (MQ=255)
                                                                                                                 aaGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCt                                  <  1:8792520/151‑1 (MQ=255)
                                                                                                                   ggCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTc                                >  1:8136255/1‑151 (MQ=255)
                                                                                                                    gCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTcc                               <  1:681770/151‑1 (MQ=255)
                                                                                                                     ccGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTccc                              >  1:1045153/1‑151 (MQ=255)
                                                                                                                      cgcgCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTcccc                             <  2:8617150/151‑1 (MQ=255)
                                                                                                                      cgcgCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTcccc                             <  2:1404492/151‑1 (MQ=255)
                                                                                                                       gcgctggcgctgGCCCAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcg                            <  1:7069020/151‑1 (MQ=255)
                                                                                                                         gctggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggc                          <  2:1223733/151‑1 (MQ=255)
                                                                                                                          ctggcgctggcCAAGGCCCTGGGGGCCGACGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggcg                         <  1:7462065/151‑1 (MQ=255)
                                                                                                                              cgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggc                     >  2:5568320/1‑151 (MQ=255)
                                                                                                                              cgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggc                     <  1:6278581/151‑1 (MQ=255)
                                                                                                                               gctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggcc                    <  2:1825034/151‑2 (MQ=255)
                                                                                                                                 tggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                  <  1:2202627/151‑1 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                >  2:9713054/1‑151 (MQ=255)
                                                                                                                                    ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt               >  1:7372473/1‑151 (MQ=255)
                                                                                                                                    ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt               <  1:4325078/151‑1 (MQ=255)
                                                                                                                                     cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg              >  2:5108710/1‑151 (MQ=255)
                                                                                                                                      aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa             >  2:6948361/1‑151 (MQ=255)
                                                                                                                                      aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa             >  1:5005617/1‑151 (MQ=255)
                                                                                                                                      aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa             <  1:6071930/151‑1 (MQ=255)
                                                                                                                                       aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGAt            <  2:4795393/151‑1 (MQ=255)
                                                                                                                                        ggCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATc           <  1:1229115/151‑1 (MQ=255)
                                                                                                                                        ggCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATc           <  1:4609599/151‑1 (MQ=255)
                                                                                                                                           ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc        <  1:1845938/151‑1 (MQ=255)
                                                                                                                                             tGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGa      >  1:5865806/1‑151 (MQ=255)
                                                                                                                                              gggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGAt     >  1:9546926/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑