Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,997,7860TC100.0% 2387.0 / NA 606K173E (AAG→GAG) PA14_RS22840DUF853 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (286/320);  total (286/320)

Only 100 of 606 total aligned reads displayed.

CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933
                                                                                                                                                    |                                                                                                                                                   
 gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGcc                                                                                                                                                  >  2:6620015/1‑151 (MQ=255)
   ccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGa                                                                                                                                                <  1:2351331/151‑1 (MQ=255)
    cccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAg                                                                                                                                               >  2:9046656/1‑151 (MQ=255)
       gcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAt                                                                                                                                            <  2:3417339/151‑1 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           <  1:10171257/151‑1 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           <  1:722366/151‑1 (MQ=255)
         ccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtct                                                                                                                                          <  2:3597599/151‑1 (MQ=255)
         ccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtct                                                                                                                                          >  1:8162392/1‑151 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        <  1:9285416/151‑1 (MQ=255)
             ccGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCCGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCgg                                                                                                                                      >  2:2036288/1‑151 (MQ=255)
               ggCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAt                                                                                                                                    <  2:9471491/151‑1 (MQ=255)
                gCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAtt                                                                                                                                   <  2:6871708/151‑1 (MQ=255)
                gCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAtt                                                                                                                                   >  1:10032235/1‑151 (MQ=255)
                  tCGGGGCGGAGGATGTCTCCCAGTTGCCGGGCAGGCTCGCCGCAGACGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCCGCAGCGCCTGCCCGGAAGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATGGt                                                                                                                                 <  2:731355/151‑1 (MQ=255)
                   cGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTc                                                                                                                                <  1:7766158/151‑1 (MQ=255)
                     gggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGGGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCt                                                                                                                              <  2:10213405/151‑1 (MQ=255)
                      ggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtg                                                                                                                             >  1:462827/1‑150 (MQ=255)
                        cGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                           >  2:1596475/1‑151 (MQ=255)
                        cGGAGGATGTCTTCCAGGTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                           <  2:7752695/151‑1 (MQ=255)
                           aggaTGTCTTCCAGGTGCAGGGCAGGCGCGCCGGAGACGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCTGCAGCGCCTGCCCGGACGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAGTGTCCTTCAggt                                                                                                                        >  2:5881781/1‑151 (MQ=255)
                            ggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTTCTCCAGAGTGGCCAGGCTGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtg                                                                                                                       >  2:7035727/1‑151 (MQ=255)
                            ggaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtg                                                                                                                       <  2:5754311/151‑1 (MQ=255)
                               tGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGtt                                                                                                                    <  1:1369618/151‑1 (MQ=255)
                                 tCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGa                                                                                                                  <  1:8563133/151‑1 (MQ=255)
                                  cTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGag                                                                                                                 <  2:6007762/151‑1 (MQ=255)
                                     ccAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcag                                                                                                              <  1:7416350/151‑1 (MQ=255)
                                      cAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcagc                                                                                                             <  1:985008/151‑1 (MQ=255)
                                       aGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcg                                                                                                            <  1:4412746/151‑1 (MQ=255)
                                        gTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAgcgc                                                                                                           <  1:2560917/151‑1 (MQ=255)
                                         ttGCAGGGCAGGCTCGCCGCAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcc                                                                                                          <  2:2428669/151‑1 (MQ=255)
                                          tGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcct                                                                                                         >  2:81266/1‑151 (MQ=255)
                                           gcagggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGcctt                                                                                                        <  1:4922654/151‑1 (MQ=255)
                                            cagggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttc                                                                                                       <  2:7231954/151‑1 (MQ=255)
                                               ggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcagg                                                                                                    >  2:4671020/1‑151 (MQ=255)
                                                  aggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcc                                                                                                 <  2:17682/151‑1 (MQ=255)
                                                   ggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCACCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcct                                                                                                >  2:920726/1‑151 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCAGCTGCTCCAGAGTGGCCAGGAGGCACATCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               <  2:2515109/151‑1 (MQ=255)
                                                     cTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttc                                                                                              >  2:993139/1‑151 (MQ=255)
                                                       cGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcag                                                                                            >  1:11051942/1‑151 (MQ=255)
                                                       cGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcag                                                                                            <  2:2945254/151‑1 (MQ=255)
                                                         ccGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcaggt                                                                                          >  1:6214364/1‑151 (MQ=255)
                                                         ccGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcaggt                                                                                          <  2:4802856/151‑1 (MQ=255)
                                                          cGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCcttcaggtccttcaggtc                                                                                         <  1:6836686/151‑1 (MQ=255)
                                                            aagaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                       <  1:5405599/151‑1 (MQ=255)
                                                            aagaagGCCTCGGCGCCCTGCTGCACCAGAGTGGACAGGCGGATCAGCAGCGCCTGCACGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCTAGGATCACCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                       >  2:5544125/1‑151 (MQ=255)
                                                                 ggCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagc                                                                                  >  1:6411616/1‑151 (MQ=255)
                                                                  gCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagca                                                                                 <  1:8882694/151‑1 (MQ=255)
                                                                    cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcagc                                                                               >  1:1558791/1‑151 (MQ=255)
                                                                    cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcagc                                                                               >  2:411997/1‑151 (MQ=255)
                                                                    cTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcagcagc                                                                               >  2:8419132/1‑151 (MQ=255)
                                                                       ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAgg                                                                            <  1:291737/151‑1 (MQ=255)
                                                                        gcgcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGc                                                                           <  1:7594440/151‑1 (MQ=255)
                                                                          gcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCt                                                                         <  1:10721827/151‑1 (MQ=255)
                                                                             ctgctgCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTcc                                                                      >  1:9753854/1‑151 (MQ=255)
                                                                               gctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCg                                                                    <  1:2234637/151‑1 (MQ=255)
                                                                                ctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCgg                                                                   <  1:4009894/151‑1 (MQ=255)
                                                                                ctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGGCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCgg                                                                   >  2:5173166/1‑151 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 <  1:3675788/151‑1 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 >  2:8846013/1‑151 (MQ=255)
                                                                                        gagTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCAc                                                           <  2:2766072/151‑1 (MQ=255)
                                                                                            ggCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTgg                                                       >  2:1247334/1‑151 (MQ=255)
                                                                                               cAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAg                                                    <  1:6699055/151‑1 (MQ=255)
                                                                                               cAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAg                                                    >  1:10344672/1‑151 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGCGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   <  2:10558227/151‑1 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCTGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   >  2:3139032/1‑151 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   >  1:3810319/1‑151 (MQ=255)
                                                                                                  gcggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcg                                                 <  2:3984718/151‑1 (MQ=255)
                                                                                                       gcagcaGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGaa                                            >  1:1599003/1‑151 (MQ=255)
                                                                                                         agcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                          >  1:7930375/1‑151 (MQ=255)
                                                                                                          gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCCGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGACCAg                                         >  2:10061853/1‑151 (MQ=255)
                                                                                                          gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAg                                         <  2:3225768/151‑1 (MQ=255)
                                                                                                          gcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAg                                         <  1:8630744/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       <  2:2205835/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       <  1:554797/151‑1 (MQ=255)
                                                                                                             gcgcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcgc                                      <  2:10898355/151‑1 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  >  2:7416167/1‑151 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  >  1:3526296/1‑151 (MQ=255)
                                                                                                                   gCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctg                                <  1:9141161/151‑1 (MQ=255)
                                                                                                                     ccGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgct                              <  2:6428944/151‑1 (MQ=255)
                                                                                                                      cGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgctg                             >  1:10518851/1‑151 (MQ=255)
                                                                                                                       ggAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTgg                            <  1:4122002/151‑1 (MQ=255)
                                                                                                                       ggAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTgg                            >  1:3092662/1‑151 (MQ=255)
                                                                                                                         aaGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCt                          <  1:9601834/151‑1 (MQ=255)
                                                                                                                            cgcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                       <  1:2746339/151‑1 (MQ=255)
                                                                                                                             gcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCg                      <  1:7094417/151‑1 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGt                    >  1:1280713/1‑151 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGt                    <  1:56475/151‑1 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGt                    >  2:3766051/1‑151 (MQ=255)
                                                                                                                               cGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGt                    >  2:1109143/1‑151 (MQ=255)
                                                                                                                                 gTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCa                  <  2:6207786/151‑1 (MQ=255)
                                                                                                                                 gTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCa                  <  1:8422855/151‑1 (MQ=255)
                                                                                                                                    aaCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACt               >  2:10418447/1‑151 (MQ=255)
                                                                                                                                     aCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTc              >  1:1795267/1‑151 (MQ=255)
                                                                                                                                       aGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCa            <  2:9765867/151‑1 (MQ=255)
                                                                                                                                        gcgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCag           <  2:7606469/151‑1 (MQ=255)
                                                                                                                                         cgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagg          >  2:1383886/1‑151 (MQ=255)
                                                                                                                                          gcCCGGGCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGCGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagga         <  2:493596/151‑1 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       >  1:5735808/1‑151 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       <  2:4514838/151‑1 (MQ=255)
                                                                                                                                                 ccTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGc  >  2:4366574/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑