Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,076,066 G→C R105R (CGG→CGC)  PA14_RS27775 → methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,076,0660GC99.8% 2128.3 / NA 552R105R (CGG→CGC) PA14_RS27775methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (294/256);  minor base A (1/0);  total (296/256)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 552 total aligned reads displayed.

GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213
                                                                                                                                                    |                                                                                                                                                   
ggCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCtt                                                                                                                                                   >  2:9581620/1‑151 (MQ=255)
  cgcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCa                                                                                                                                                 <  1:6954346/151‑1 (MQ=255)
    cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                               >  1:4142931/1‑151 (MQ=255)
    cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                               >  1:6133232/1‑151 (MQ=255)
    cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                               >  1:8622334/1‑151 (MQ=255)
     aGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATg                                                                                                                                              <  1:8431853/151‑1 (MQ=255)
      gCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                             <  1:10281649/151‑1 (MQ=255)
      gCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                             <  2:5759878/151‑1 (MQ=255)
       ccTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTc                                                                                                                                            >  2:3/1‑151 (MQ=255)
        cTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCg                                                                                                                                           <  1:4570265/151‑1 (MQ=255)
          cGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGAc                                                                                                                                         <  1:9171953/151‑1 (MQ=255)
            caccggCCGACTCGTAGTACTATCCGCCCAGGCGGCGGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGt                                                                                                                                       <  1:6639469/147‑1 (MQ=255)
            aaCAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGt                                                                                                                                       <  2:5290987/151‑1 (MQ=255)
                   cGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGa                                                                                                                                <  1:5669710/151‑1 (MQ=255)
                     aCGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTTCCGGGACg                                                                                                                              >  2:10709845/1‑151 (MQ=255)
                       gcgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCa                                                                                                                            >  1:6855463/1‑151 (MQ=255)
                       gcgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCa                                                                                                                            <  2:8062179/151‑1 (MQ=255)
                         ggAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGGACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAg                                                                                                                          >  1:2587590/1‑151 (MQ=255)
                            gccccaCCCGCCCACCCGCCACGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCCGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCACTGTCGACGTACCGGGACGCAAGTgg                                                                                                                       <  2:10882838/146‑1 (MQ=255)
                               caACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcag                                                                                                                    <  2:9659111/151‑1 (MQ=255)
                                aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagc                                                                                                                   >  2:10606656/1‑151 (MQ=255)
                                aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagc                                                                                                                   >  2:7394736/1‑151 (MQ=255)
                                 aCCTGCCCTCGCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagca                                                                                                                  <  2:2669479/151‑1 (MQ=255)
                                  ccAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGcatcag                                                                                                                 <  2:10093677/151‑1 (MQ=255)
                                    accccAGCCCCCTGGCCGCTCCCGAGCGCTATCCGCAGCTCGCCGCCCAGGCCGCTGCGGTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGAt                                                                                                               <  1:2976587/149‑1 (MQ=255)
                                           ccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTg                                                                                                        <  1:529513/151‑1 (MQ=255)
                                             gCCTGGGCGCGCACGCGCCGTATCCGCAGCTCGCCGCCCAGGGCGCAGCGCTGGGCGCGGTCGGCGAACTGCCGTTGGCGGAACGCCCGCAGGCACCGAACCGCGTCAATGTCGACGTACCGGGGCGCAAGTGGGAGCAGATCGGCCGGtg                                                                                                      >  2:667706/1‑150 (MQ=255)
                                                tGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGc                                                                                                   >  2:882732/1‑151 (MQ=255)
                                                 ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                  >  2:1730176/1‑151 (MQ=255)
                                                    cgcgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCggg                                                                                               <  1:48310/151‑1 (MQ=255)
                                                     gcgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgc                                                                                              <  2:1571045/151‑1 (MQ=255)
                                                          cgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTg                                                                                         <  2:3278415/151‑1 (MQ=255)
                                                           gcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTACAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGc                                                                                        <  2:7700270/151‑1 (MQ=255)
                                                             gccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAg                                                                                      >  1:8786503/1‑151 (MQ=255)
                                                                ttATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTc                                                                                   >  1:3776841/1‑151 (MQ=255)
                                                                  aTCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGc                                                                                 >  1:6519886/1‑151 (MQ=255)
                                                                   tCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                                >  1:1859035/1‑151 (MQ=255)
                                                                   tCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                                <  2:10326432/151‑1 (MQ=255)
                                                                    ccGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGccc                                                                               <  1:10038410/151‑1 (MQ=255)
                                                                       cAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggc                                                                            <  1:10645278/151‑1 (MQ=255)
                                                                       cAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggc                                                                            <  1:7847330/151‑1 (MQ=255)
                                                                           tCGCCGCCCAGGCCGCAGCGCTGCGCGAGGTCGGCGAACTGCCATAGGCGGAACGCCCGCAGGCACCGACCCGCTTCAAGGTCGACGTACCGGGACGCCAGTGCCAGCAGATCGGCCTGTACGCAGGGCGCCTGGAGTTCGCCCGGCCGgt                                                                        <  2:7170223/151‑1 (MQ=255)
                                                                              ccgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgt                                                                     >  2:10999307/1‑151 (MQ=255)
                                                                                gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGAGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTACGCCCGGCCGGTCGTcc                                                                   >  2:5002828/1‑151 (MQ=255)
                                                                                 cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCa                                                                  >  2:822048/1‑151 (MQ=255)
                                                                                   cAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCAcg                                                                >  1:3881606/1‑150 (MQ=255)
                                                                                    aGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTg                                                               >  2:6258803/1‑151 (MQ=255)
                                                                                        cGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTc                                                           <  2:5424638/151‑1 (MQ=255)
                                                                                        cGCAGCGCGGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTc                                                           <  2:6506017/151‑1 (MQ=255)
                                                                                            gcgcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACt                                                       <  1:7632825/151‑1 (MQ=255)
                                                                                             cgcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCGGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTg                                                      >  2:9364915/1‑151 (MQ=255)
                                                                                              gcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTgg                                                     >  2:6787176/1‑151 (MQ=255)
                                                                                               cTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGt                                                    >  2:4776798/1‑151 (MQ=255)
                                                                                               cTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGt                                                    <  1:7259910/151‑1 (MQ=255)
                                                                                                tGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                   >  1:1530684/1‑151 (MQ=255)
                                                                                                   gcgcGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTc                                                >  2:5681097/1‑151 (MQ=255)
                                                                                                     gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCg                                              <  1:3695135/151‑1 (MQ=255)
                                                                                                     gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCg                                              <  1:2830776/151‑1 (MQ=255)
                                                                                                         tCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCaa                                          >  1:9673546/1‑151 (MQ=255)
                                                                                                         tCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTCCAAGGTCGACGGACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCaa                                          <  2:8941915/151‑1 (MQ=255)
                                                                                                            gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAggg                                       <  2:9247001/151‑1 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     >  2:2302172/1‑151 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     >  2:6007819/1‑151 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     >  2:1275805/1‑151 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     <  1:2445635/151‑1 (MQ=255)
                                                                                                              gAACTGCCATGGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     <  2:8635525/151‑1 (MQ=255)
                                                                                                               aaCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCa                                    >  2:9138136/1‑151 (MQ=255)
                                                                                                                 cTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATc                                  <  1:11083965/151‑1 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCACGGGCCATCt                                 >  2:10003554/1‑151 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 <  1:626662/151‑1 (MQ=255)
                                                                                                                   gCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTg                                <  2:11097924/151‑1 (MQ=255)
                                                                                                                    ccATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTgg                               <  1:5175690/151‑1 (MQ=255)
                                                                                                                     cATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTggg                              <  2:7842552/151‑1 (MQ=255)
                                                                                                                     cATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTggg                              >  1:7149551/1‑151 (MQ=255)
                                                                                                                      aTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCCTCTGGgc                             >  2:1939682/1‑151 (MQ=255)
                                                                                                                        tGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccg                           >  1:5552324/1‑151 (MQ=255)
                                                                                                                         ggCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccgc                          >  2:6714250/1‑151 (MQ=255)
                                                                                                                         ggCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccgc                          <  1:3386768/151‑1 (MQ=255)
                                                                                                                            ggAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                       >  1:801327/1‑151 (MQ=255)
                                                                                                                            ggAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                       >  1:9920528/1‑151 (MQ=255)
                                                                                                                              aaCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgct                     <  2:4664822/151‑1 (MQ=255)
                                                                                                                                cGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTgg                   >  1:2165251/1‑151 (MQ=255)
                                                                                                                                 gCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGc                  <  1:3107764/151‑1 (MQ=255)
                                                                                                                                   ccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGccc                >  2:1908933/1‑151 (MQ=255)
                                                                                                                                     gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCAt              >  2:8457662/1‑151 (MQ=255)
                                                                                                                                     gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCAt              <  2:3393933/151‑1 (MQ=255)
                                                                                                                                     gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCAt              <  2:2454852/151‑1 (MQ=255)
                                                                                                                                     gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGGCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCAt              >  1:5903682/1‑151 (MQ=255)
                                                                                                                                      cAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg             <  1:6368528/151‑1 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         <  1:4302105/151‑1 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         >  2:3684746/1‑151 (MQ=255)
                                                                                                                                           aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        >  2:4523744/1‑151 (MQ=255)
                                                                                                                                           aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        >  1:1034640/1‑151 (MQ=255)
                                                                                                                                            ccGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGc       <  2:6995133/151‑1 (MQ=255)
                                                                                                                                            ccGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGc       <  2:447291/151‑1 (MQ=255)
                                                                                                                                            ccGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGc       >  2:3984910/1‑151 (MQ=255)
                                                                                                                                              gAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCaa     <  1:4366669/151‑1 (MQ=255)
                                                                                                                                              gAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCaa     <  1:10161257/151‑1 (MQ=255)
                                                                                                                                               caCCGCTTCCATGTCGACGCACCGGGACGCAAGTGGCAGCAGATCGGCCTGGTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAc    <  2:5097841/150‑1 (MQ=255)
                                                                                                                                                 ccGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCAGGGCCGCCAGCTGGCCCATGCGGGGCAACCg  >  1:10947234/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑