Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846372,4400TC99.8% 1905.7 / NA 477K132E (AAG→GAG) PA14_RS00315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (189/287);  minor base A (1/0);  total (190/287)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587
                                                                                                                                                    |                                                                                                                                                   
cGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGc                                                                                                                                                   <  1:8953055/151‑1 (MQ=255)
 ggCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTcgcg                                                                                                                                                  <  1:10267945/151‑1 (MQ=255)
   cAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGc                                                                                                                                                >  2:286899/1‑151 (MQ=255)
   cAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGc                                                                                                                                                <  1:803389/151‑1 (MQ=255)
       ggaccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTACGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                            <  2:352853/148‑1 (MQ=255)
       ggTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                            >  2:8201113/1‑151 (MQ=255)
              cgcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAt                                                                                                                                     <  2:1956763/151‑1 (MQ=255)
               gcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAtt                                                                                                                                    <  1:11015617/151‑1 (MQ=255)
                cAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTc                                                                                                                                   <  1:7711598/151‑1 (MQ=255)
                cAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGACACGGGAGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCACCTCCTACCATCGCGCCCTCGCCTTGCGCCGATCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTc                                                                                                                                   <  1:3923258/151‑1 (MQ=255)
                  gCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTccc                                                                                                                                 <  1:1444801/151‑1 (MQ=255)
                    cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtg                                                                                                                               <  1:2969768/151‑1 (MQ=255)
                       cGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGAGCCAATTCCCtgttg                                                                                                                            >  2:10847372/1‑151 (MQ=255)
                        gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttgt                                                                                                                           >  2:10989744/1‑151 (MQ=255)
                          ggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTcc                                                                                                                         >  1:2099142/1‑151 (MQ=255)
                           gagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTcat                                                                                                                        >  1:1123350/1‑149 (MQ=255)
                               agcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTc                                                                                                                    <  2:5269980/151‑1 (MQ=255)
                                  agcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTa                                                                                                                 <  2:7063602/151‑1 (MQ=255)
                                  agcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTa                                                                                                                 <  2:10687074/151‑1 (MQ=255)
                                   gcgcGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTag                                                                                                                <  1:6889192/151‑1 (MQ=255)
                                    cgcgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTaga                                                                                                               <  2:2175490/151‑1 (MQ=255)
                                         gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTc                                                                                                          <  2:7953513/151‑1 (MQ=255)
                                         gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTc                                                                                                          <  1:1098056/151‑1 (MQ=255)
                                           gggACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGc                                                                                                        >  1:8814341/1‑151 (MQ=255)
                                            ggACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGcc                                                                                                       >  2:5439737/1‑151 (MQ=255)
                                                gagaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAgg                                                                                                   >  1:3693465/1‑151 (MQ=255)
                                                 agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                  <  1:4473241/151‑1 (MQ=255)
                                                 agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                  <  1:723490/151‑1 (MQ=255)
                                                  gaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                 <  1:4010682/151‑1 (MQ=255)
                                                  gaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                 <  2:1686007/151‑1 (MQ=255)
                                                   aCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGAc                                                                                                <  1:1647681/151‑1 (MQ=255)
                                                    cGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGCCg                                                                                               >  1:5057488/1‑151 (MQ=255)
                                                    cGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                               >  1:5615491/1‑151 (MQ=255)
                                                    cGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                               >  1:6627967/1‑151 (MQ=255)
                                                    cGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                               >  1:3130239/1‑151 (MQ=255)
                                                      ggTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGa                                                                                             <  1:868779/151‑1 (MQ=255)
                                                        tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                           >  1:1324565/1‑151 (MQ=255)
                                                        tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                           <  2:11015808/151‑1 (MQ=255)
                                                          aGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                         >  1:6678192/1‑151 (MQ=255)
                                                          aGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                         <  2:3978539/151‑1 (MQ=255)
                                                            tGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGa                                                                                       >  1:6047245/1‑151 (MQ=255)
                                                             ggACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAt                                                                                      <  1:3860121/151‑1 (MQ=255)
                                                               aCGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgt                                                                                    <  2:4718585/151‑1 (MQ=255)
                                                               aCGCATGGGGCGGGCGCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgt                                                                                    <  2:8413800/151‑1 (MQ=255)
                                                                 gcgtGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAGTCCCTGTTGTCCTGGTCGGAGAGCTTCGCCCAGGGACGGAAGCCGATGTGc                                                                                  >  1:10729998/4‑151 (MQ=255)
                                                                 gCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGc                                                                                  <  1:1701095/151‑1 (MQ=255)
                                                                 gCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGc                                                                                  <  1:8215111/151‑1 (MQ=255)
                                                                 gCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGc                                                                                  <  1:7425139/151‑1 (MQ=255)
                                                                  cATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGcc                                                                                 >  1:7833704/1‑151 (MQ=255)
                                                                     ggggCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCgg                                                                              >  2:8619363/1‑151 (MQ=255)
                                                                      gggcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGc                                                                             <  1:2677856/151‑1 (MQ=255)
                                                                        gcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCt                                                                           <  1:5850120/151‑1 (MQ=255)
                                                                         cgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTc                                                                          >  2:6817371/1‑151 (MQ=255)
                                                                         cgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTc                                                                          >  1:10160546/1‑151 (MQ=255)
                                                                          gggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTcc                                                                         >  1:9767680/1‑151 (MQ=255)
                                                                           ggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCa                                                                        >  1:984713/1‑151 (MQ=255)
                                                                             cTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                      <  1:6434195/151‑1 (MQ=255)
                                                                              tCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGc                                                                     <  2:8892109/151‑1 (MQ=255)
                                                                              tCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGc                                                                     >  1:9067314/1‑151 (MQ=255)
                                                                              tCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGc                                                                     <  1:3417720/151‑1 (MQ=255)
                                                                                 ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                  <  1:10021633/151‑1 (MQ=255)
                                                                                   aGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                                <  2:1203276/151‑1 (MQ=255)
                                                                                   aGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                                <  1:4236383/151‑1 (MQ=255)
                                                                                    gggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgc                                                               >  2:5914324/1‑151 (MQ=255)
                                                                                      gCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGACCGGCCTCCAGGCGGTAgcgc                                                             >  2:10236602/1‑151 (MQ=255)
                                                                                          tCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                         >  2:9547237/1‑151 (MQ=255)
                                                                                           cAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGcc                                                        >  1:5767872/1‑151 (MQ=255)
                                                                                             gCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGGCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAGGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                      <  2:3361646/151‑1 (MQ=255)
                                                                                              cTCCGAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCCGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgc                                                     <  2:10484399/151‑1 (MQ=255)
                                                                                               gccTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcg                                                    >  1:2769154/2‑151 (MQ=255)
                                                                                                ccTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcgc                                                   >  1:5236473/1‑151 (MQ=255)
                                                                                                 cgaGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                  >  2:4498171/3‑151 (MQ=255)
                                                                                                 cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                  >  1:2383273/1‑151 (MQ=255)
                                                                                                  tAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTg                                                 <  2:7189335/151‑1 (MQ=255)
                                                                                                    gCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                               >  2:3113429/1‑151 (MQ=255)
                                                                                                    gCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                               <  2:7604026/151‑1 (MQ=255)
                                                                                                       tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTc                                            <  2:1861555/151‑1 (MQ=255)
                                                                                                         gcgcCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCt                                          <  1:759608/151‑1 (MQ=255)
                                                                                                          cgcCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCACGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCtt                                         <  1:4873103/151‑1 (MQ=255)
                                                                                                            cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                       <  2:4546502/151‑1 (MQ=255)
                                                                                                            cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                       >  1:1980138/1‑151 (MQ=255)
                                                                                                              cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAAc                                     >  1:8547530/1‑151 (MQ=255)
                                                                                                              cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAAc                                     >  2:749535/1‑151 (MQ=255)
                                                                                                                cGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCATTTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGACGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                   <  2:2613887/151‑1 (MQ=255)
                                                                                                                cGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                   <  1:4102378/151‑1 (MQ=255)
                                                                                                                cGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                   <  2:2426327/151‑1 (MQ=255)
                                                                                                                  ccTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                 <  2:6290514/151‑1 (MQ=255)
                                                                                                                  ccTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                 <  1:4983575/151‑1 (MQ=255)
                                                                                                                           gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                        >  2:6588642/1‑151 (MQ=255)
                                                                                                                            ttCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTg                       <  2:1332324/151‑1 (MQ=255)
                                                                                                                                   cTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTGGAACTTGAGGTTGAGCTGGCAATcg                >  2:4944358/1‑151 (MQ=255)
                                                                                                                                    ttCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcgc               <  2:5634915/151‑1 (MQ=255)
                                                                                                                                    ttCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcgc               <  1:10539163/151‑1 (MQ=255)
                                                                                                                                     tCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcgcg              <  1:1199973/151‑1 (MQ=255)
                                                                                                                                      cgcgCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCgcgc             >  1:6713172/1‑151 (MQ=255)
                                                                                                                                      cgcgCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCgcgc             >  2:7743714/1‑151 (MQ=255)
                                                                                                                                       gcgcAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGcc            >  2:9212285/1‑151 (MQ=255)
                                                                                                                                           aGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCagag        <  1:9422150/151‑1 (MQ=255)
                                                                                                                                               ccccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGACGGCTCTGAAGTCCTAGTCCTGGAGGTTGAGCTGGAACTCGAGCCAGAGCggg    >  2:3769159/1‑151 (MQ=255)
                                                                                                                                                 ccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCt  >  1:8329202/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑