Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,558,205 A→G Q390Q (CAA→CAG)  PA14_RS07290 → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,558,2050AG99.8% 1916.6 / NA 486Q390Q (CAA→CAG) PA14_RS07290propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (243/241);  minor base T (0/1);  total (243/243)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 492 total aligned reads displayed.

GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352
                                                                                                                                                    |                                                                                                                                                   
 cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                  <  1:7890282/151‑1 (MQ=255)
 cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                  <  1:2145267/151‑1 (MQ=255)
 cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                  >  2:6085601/1‑151 (MQ=255)
  aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCGTGGACCATTGGTGGCAGACCg                                                                                                                                                 <  1:7689356/151‑1 (MQ=255)
     gAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgaga                                                                                                                                              <  2:1635006/151‑1 (MQ=255)
     gAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCGAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgaga                                                                                                                                              <  2:7618490/151‑1 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            <  2:8023668/151‑1 (MQ=255)
        gACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCagaccgagaccg                                                                                                                                           <  1:1637767/151‑1 (MQ=255)
          cccGCATGGCGAACGGGTGACGCGCTACGAACTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGc                                                                                                                                         >  1:385429/1‑151 (MQ=255)
             gCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTgg                                                                                                                                      <  1:7117608/151‑1 (MQ=255)
               aTGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGcc                                                                                                                                    >  1:8420230/1‑151 (MQ=255)
                  gCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGt                                                                                                                                 <  2:6849174/151‑1 (MQ=255)
                   cGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                                <  2:10542939/151‑1 (MQ=255)
                    gAACGGGTGAAGCGCTACGACCTAAGGTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                               <  1:3085322/151‑1 (MQ=255)
                      aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                             <  1:10836138/151‑1 (MQ=255)
                      aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                             <  1:1060632/151‑1 (MQ=255)
                      aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGGTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                             <  2:2505960/151‑1 (MQ=255)
                        gggTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCAGTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgc                                                                                                                           >  2:9337892/1‑151 (MQ=255)
                              aGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTg                                                                                                                     <  1:3703415/151‑1 (MQ=255)
                               gcgcTACGACCTAAGGTCGCCGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCCGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATGGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGc                                                                                                                    <  2:3967720/151‑1 (MQ=255)
                                cgcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCa                                                                                                                   >  1:7365133/1‑151 (MQ=255)
                                  cTACGACCTAAGTTCGCTGCGCCACCTGGTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATc                                                                                                                 <  2:10451430/151‑1 (MQ=255)
                                   tACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCGGGCCGGTGACCGCGCCCTGCATCg                                                                                                                >  2:7804963/1‑151 (MQ=255)
                                      gACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGTGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGa                                                                                                             >  2:5765625/1‑151 (MQ=255)
                                          tAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                         >  2:5283588/1‑151 (MQ=255)
                                           aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGcc                                                                                                        >  2:5008911/1‑151 (MQ=255)
                                               tCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTTGTGGCAGACCGAGAACGGCTGGCCGGTGACCGCGCACTGCATCGTGAGCGCCGGcc                                                                                                    >  1:8149484/1‑151 (MQ=255)
                                               tCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGcc                                                                                                    <  1:8309838/151‑1 (MQ=255)
                                                  cTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACg                                                                                                 <  2:2812878/151‑1 (MQ=255)
                                                  cTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACg                                                                                                 <  1:122302/151‑1 (MQ=255)
                                                  cTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACg                                                                                                 <  2:2276628/151‑1 (MQ=255)
                                                      gcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCt                                                                                             >  1:2352103/1‑151 (MQ=255)
                                                       ccACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTg                                                                                            >  1:8629489/1‑151 (MQ=255)
                                                         aCCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                          <  1:1105142/151‑1 (MQ=255)
                                                         aCCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                          <  1:5686258/151‑1 (MQ=255)
                                                           cTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCggg                                                                                        <  2:8357535/151‑1 (MQ=255)
                                                            tGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGc                                                                                       <  2:4280867/151‑1 (MQ=255)
                                                             gTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGcc                                                                                      <  1:9602402/151‑1 (MQ=255)
                                                               tCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCgg                                                                                    <  2:9114670/151‑1 (MQ=255)
                                                                 cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                  >  1:2008196/1‑151 (MQ=255)
                                                                 cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                  <  1:3368886/151‑1 (MQ=255)
                                                                  tGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTc                                                                                 <  1:9181471/151‑1 (MQ=255)
                                                                   ggCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCg                                                                                >  2:5656058/1‑151 (MQ=255)
                                                                   ggCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCg                                                                                >  2:3375711/1‑151 (MQ=255)
                                                                    gCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGa                                                                               >  2:4554411/1‑151 (MQ=255)
                                                                    gCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGa                                                                               >  2:8935939/1‑151 (MQ=255)
                                                                      cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                             >  2:10317712/1‑151 (MQ=255)
                                                                      cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                             >  1:6120723/1‑151 (MQ=255)
                                                                      cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                             >  2:10952503/1‑151 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            >  1:750683/1‑151 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            >  1:5933447/1‑151 (MQ=255)
                                                                             aaGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcg                                                                      <  1:10026346/151‑1 (MQ=255)
                                                                             aaGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcg                                                                      >  2:5920836/1‑151 (MQ=255)
                                                                              aGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgc                                                                     >  2:5144854/1‑151 (MQ=255)
                                                                                cTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                   <  1:5194612/151‑1 (MQ=255)
                                                                                cTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                   >  2:2997312/1‑151 (MQ=255)
                                                                                 tCGACAGCAGCACCCAGCACTGGCTGGACGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                  <  2:10069150/151‑1 (MQ=255)
                                                                                 tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                  <  1:1849528/151‑1 (MQ=255)
                                                                                  cGACAGCCGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGGCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGGGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTg                                                                 <  2:7436917/151‑1 (MQ=255)
                                                                                    aCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGcc                                                               >  1:1572976/1‑151 (MQ=255)
                                                                                    aCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGcc                                                               >  1:2611813/1‑151 (MQ=255)
                                                                                     cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGGGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                              <  2:10762478/151‑1 (MQ=255)
                                                                                         agcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCt                                                          >  2:3681524/1‑151 (MQ=255)
                                                                                          gcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTa                                                         <  2:150877/151‑1 (MQ=255)
                                                                                               cAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACg                                                    <  1:9727295/151‑1 (MQ=255)
                                                                                                 gCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTg                                                  <  1:2941054/151‑1 (MQ=255)
                                                                                                  cACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGc                                                 <  1:6278066/151‑1 (MQ=255)
                                                                                                   aCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCa                                                >  2:2348218/1‑151 (MQ=255)
                                                                                                     tggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGGTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                              >  2:9822601/1‑151 (MQ=255)
                                                                                                      ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                             <  1:7401496/151‑1 (MQ=255)
                                                                                                      ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                             <  1:4823963/151‑1 (MQ=255)
                                                                                                       gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                            >  2:6309688/1‑151 (MQ=255)
                                                                                                       gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                            <  1:5492493/151‑1 (MQ=255)
                                                                                                        ctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGc                                           >  2:9448535/1‑151 (MQ=255)
                                                                                                           gatgagCACACCGGGAGGCCGGTCCACGCCCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCCCGTGCAGGTGCTgg                                        <  1:4392598/148‑1 (MQ=255)
                                                                                                           gAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTgg                                        >  1:5402975/1‑151 (MQ=255)
                                                                                                            aaGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGga                                       >  2:7227335/1‑151 (MQ=255)
                                                                                                            aaGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGga                                       <  2:6645244/151‑1 (MQ=255)
                                                                                                             agagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgac                                      <  2:1184173/151‑1 (MQ=255)
                                                                                                              gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacg                                     <  2:3808577/151‑1 (MQ=255)
                                                                                                              gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacg                                     >  2:7056319/1‑151 (MQ=255)
                                                                                                                gCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacgac                                   <  2:2083164/151‑1 (MQ=255)
                                                                                                                 cacaCCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGacgacg                                  >  2:4781355/1‑151 (MQ=255)
                                                                                                                  acacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGAcgacga                                 >  1:6778896/1‑151 (MQ=255)
                                                                                                                     ccGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAgg                              <  1:5254528/151‑1 (MQ=255)
                                                                                                                     ccGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAgg                              <  1:9034283/151‑1 (MQ=255)
                                                                                                                        ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                           <  1:9967797/151‑1 (MQ=255)
                                                                                                                         gcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCACCCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGTCGCAggccgg                          >  2:2926317/1‑151 (MQ=255)
                                                                                                                         gcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccgg                          >  2:8490771/1‑151 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        >  1:5998946/1‑151 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        >  1:4617622/1‑151 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        >  1:4111166/1‑151 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        >  1:2812774/1‑151 (MQ=255)
                                                                                                                               ggTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTg                    >  1:3510332/1‑151 (MQ=255)
                                                                                                                                  ccACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGa                 <  1:4022787/151‑1 (MQ=255)
                                                                                                                                  ccACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGa                 <  1:10226555/151‑1 (MQ=255)
                                                                                                                                      gACCATTGGTGGCAGAACGAGACCGGATTGCCGGAGACCGCGCCCTGCATCGGGATCGCCGGCCAAGACCTGCGGTCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGa             >  2:8783784/1‑151 (MQ=255)
                                                                                                                                      gACCATTGGAGGCACACCGAGACCGGCAGGCCGGTGCCCGCTCCCTGCATCGGGACCGCCGGCCACGACCTGCGGGCCGGTTCGACCACCCGCGCGGTGCCCGGCTACCACGTGCAGGTGCCGGACGACGAAGGCCGGCCGCTGGGAGCGa             <  2:964671/151‑1 (MQ=255)
                                                                                                                                        ccATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAc           <  1:8793565/151‑1 (MQ=255)
                                                                                                                                         cATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAcc          <  2:1861758/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑