Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,651,339 T→C F68L (TTC→CTC)  PA14_RS12480 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,651,3390TC100.0% 1925.0 / NA 491F68L (TTC→CTC) PA14_RS12480MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (224/267);  total (224/267)

Only 100 of 491 total aligned reads displayed.

GGTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGC  >  NC_008463/2651191‑2651486
                                                                                                                                                    |                                                                                                                                                   
ggtggCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTc                                                                                                                                                   >  1:7410405/1‑151 (MQ=255)
 gtggCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCa                                                                                                                                                  >  1:7271718/1‑151 (MQ=255)
    gCGTTCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGGTCATCGCACCGATGAGCGCCGAGGCCGCCGGGGACCGCGCCGCGGTGTCGTTGCGGGTGGCGCGGTTCGGAGTGGCGGTCGCCAGCACCAGCCCGCTCAAgg                                                                                                                                               >  2:9121873/1‑151 (MQ=255)
           tGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggctggct                                                                                                                                        >  2:14808/1‑151 (MQ=255)
           tGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGggctggct                                                                                                                                        <  1:5178040/151‑1 (MQ=255)
             cTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGa                                                                                                                                      <  1:160251/151‑1 (MQ=255)
             cTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGa                                                                                                                                      >  2:10088459/1‑151 (MQ=255)
              tCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGAt                                                                                                                                     <  1:9963302/151‑1 (MQ=255)
                   cccACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGAcc                                                                                                                                <  1:438262/151‑1 (MQ=255)
                      aCCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGtt                                                                                                                             >  1:7156282/1‑151 (MQ=255)
                       ccGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTg                                                                                                                            <  2:8812085/151‑1 (MQ=255)
                         ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTggg                                                                                                                          >  2:4396915/1‑151 (MQ=255)
                          gCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgggg                                                                                                                         <  2:5696520/151‑1 (MQ=255)
                                 gctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcgg                                                                                                                  <  1:10267960/151‑1 (MQ=255)
                                  ctgAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCgcggc                                                                                                                 >  2:2800249/1‑151 (MQ=255)
                                    gAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGcggcgg                                                                                                               >  1:9302902/1‑151 (MQ=255)
                                      acacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGAc                                                                                                             >  1:3452849/1‑151 (MQ=255)
                                       cacCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACa                                                                                                            <  1:8911121/151‑1 (MQ=255)
                                         ccTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                          >  2:5435200/1‑151 (MQ=255)
                                           ttCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGc                                                                                                        <  1:6891318/151‑1 (MQ=255)
                                           ttCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGc                                                                                                        <  2:6616489/151‑1 (MQ=255)
                                           ttCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGc                                                                                                        >  1:1787348/1‑151 (MQ=255)
                                            tCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCt                                                                                                       >  1:4787229/1‑151 (MQ=255)
                                              aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCg                                                                                                     >  1:10149600/1‑151 (MQ=255)
                                               gTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                    <  2:4863707/151‑1 (MQ=255)
                                               gTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCgg                                                                                                    <  1:2366642/151‑1 (MQ=255)
                                                tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCggg                                                                                                   <  2:969359/151‑1 (MQ=255)
                                                tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTGGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCggg                                                                                                   <  2:4198182/151‑1 (MQ=255)
                                                  tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCt                                                                                                 <  2:6230989/151‑1 (MQ=255)
                                                      cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                             <  1:4684806/151‑1 (MQ=255)
                                                      cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                             >  2:9540645/1‑151 (MQ=255)
                                                         cGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACcgcg                                                                                          <  1:6990335/151‑1 (MQ=255)
                                                          gCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCgg                                                                                         >  2:7681659/1‑151 (MQ=255)
                                                             ccGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGccc                                                                                      >  1:10640907/1‑151 (MQ=255)
                                                              cGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCt                                                                                     >  2:1614325/1‑151 (MQ=255)
                                                               gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                    <  1:3071938/151‑1 (MQ=255)
                                                               gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGGGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAGGCTCGGGCTGACCGCGGCCCTg                                                                                    >  1:6639615/1‑151 (MQ=255)
                                                                aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGt                                                                                   >  2:6370031/1‑151 (MQ=255)
                                                                 tGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTc                                                                                  <  2:9840193/151‑1 (MQ=255)
                                                                    gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGATGGGGCGCGCCGCGGAATAAGCTCGGGCTGACCGCGGCACTggagct                                                                               >  1:6802735/1‑146 (MQ=255)
                                                                      gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                             <  2:3328557/151‑1 (MQ=255)
                                                                       ccGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgcc                                                                            <  1:1315631/151‑1 (MQ=255)
                                                                        cGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccg                                                                           >  1:6309105/1‑151 (MQ=255)
                                                                          aGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgct                                                                         >  2:4569204/1‑151 (MQ=255)
                                                                           gTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgctg                                                                        >  2:4321279/1‑151 (MQ=255)
                                                                           gTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgccgctg                                                                        >  1:8505209/1‑151 (MQ=255)
                                                                             aCGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCgctgct                                                                      <  2:6515501/151‑1 (MQ=255)
                                                                               gCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCg                                                                    <  2:8127145/151‑1 (MQ=255)
                                                                                ccTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCgg                                                                   >  2:5043665/1‑151 (MQ=255)
                                                                                 cTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGc                                                                  >  1:4088496/1‑151 (MQ=255)
                                                                                   gggACCGCGCCGAGGTGTCGTTGATGTTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTTATCGACCGTTGGGGAGCGCGGCGGACAATGCTCGGGCAGACCGCGGCACTGTCGATGCCGCTTCTCGGCCt                                                                >  2:1870030/1‑151 (MQ=255)
                                                                                       ccGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcg                                                            <  2:10251902/151‑1 (MQ=255)
                                                                                           gcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGcggcgg                                                        <  2:6845392/151‑1 (MQ=255)
                                                                                           gcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGcggcgg                                                        >  2:2059698/1‑151 (MQ=255)
                                                                                            ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                       >  2:2967427/1‑151 (MQ=255)
                                                                                             cGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                      <  1:9694380/151‑1 (MQ=255)
                                                                                             cGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                      <  2:3859034/151‑1 (MQ=255)
                                                                                              gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGa                                                     >  2:5961329/1‑151 (MQ=255)
                                                                                                ggTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGGGAAt                                                   >  1:8346671/1‑151 (MQ=255)
                                                                                                 gtgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATg                                                  <  1:5963968/151‑1 (MQ=255)
                                                                                                    tCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCg                                               <  2:10367014/151‑1 (MQ=255)
                                                                                                     cGTTGCCGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCCGCCCGCTCAAGGGATGGCTGATCGACCGTTGGGGCGCGCCGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCACGCACTGGCGGCGGTGCAAGccct                                              >  2:10147053/1‑149 (MQ=255)
                                                                                                      gTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTc                                             >  1:2720928/1‑151 (MQ=255)
                                                                                                       ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCt                                            <  2:2415623/151‑1 (MQ=255)
                                                                                                       ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCt                                            >  2:3192341/1‑151 (MQ=255)
                                                                                                          cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGc                                         >  2:5623835/1‑151 (MQ=255)
                                                                                                          cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCACCTCGGCCTGGCGGCGGTGCATGCCGTCTGGc                                         >  2:89745/1‑151 (MQ=255)
                                                                                                           tGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCa                                        >  2:7162195/1‑151 (MQ=255)
                                                                                                              tGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAct                                     <  1:10911389/151‑1 (MQ=255)
                                                                                                               gACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAActc                                    <  1:10075932/151‑1 (MQ=255)
                                                                                                                 cGCTGTTCGGCCTGGCGGTCGCCATCACCCGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACCATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCGCGGCCTGGCGGCGGGGAATGCCGTCTGGCAACTCTa                                  <  2:7808474/151‑1 (MQ=255)
                                                                                                                   cggTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAAGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcg                                <  2:9314294/149‑1 (MQ=255)
                                                                                                                   cTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcg                                <  1:2979935/151‑1 (MQ=255)
                                                                                                                   cTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGACTGGCAACTCTAcg                                >  1:7780779/1‑151 (MQ=255)
                                                                                                                    tGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                               >  2:8210143/1‑151 (MQ=255)
                                                                                                                    tGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgc                               >  1:2439498/1‑151 (MQ=255)
                                                                                                                     gTTCGGACTGGCGTTCGCCATCACCAGCCCGCTCAATGGCTGGCAGATAGACCGTTGGTGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcgcg                              <  2:119835/151‑1 (MQ=255)
                                                                                                                      ttaggACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCAGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACgcgc                             >  1:6378853/4‑151 (MQ=255)
                                                                                                                      ttCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTTCgcgc                             >  1:7976961/1‑151 (MQ=255)
                                                                                                                       tCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                            >  2:1292572/1‑151 (MQ=255)
                                                                                                                        cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTg                           <  1:209449/151‑1 (MQ=255)
                                                                                                                           aCTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTc                        <  1:1875974/151‑1 (MQ=255)
                                                                                                                             tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                      <  2:1719978/151‑1 (MQ=255)
                                                                                                                             tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                      >  2:7905507/1‑151 (MQ=255)
                                                                                                                                 ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgctg                  >  2:5294795/1‑151 (MQ=255)
                                                                                                                                   tCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAt                <  2:7521833/151‑1 (MQ=255)
                                                                                                                                     gCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCTACTCTACGCGCTGTTCCTGCTGATCg              >  1:32703/1‑151 (MQ=255)
                                                                                                                                     gCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAGGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAtag              <  1:8828215/151‑3 (MQ=255)
                                                                                                                                       cATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc            >  2:5447041/1‑151 (MQ=255)
                                                                                                                                         tCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCct          <  1:7724958/151‑1 (MQ=255)
                                                                                                                                         tCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCct          <  2:7319187/151‑1 (MQ=255)
                                                                                                                                         tCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCct          <  1:384256/151‑1 (MQ=255)
                                                                                                                                          cACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctg         <  1:9162287/151‑1 (MQ=255)
                                                                                                                                          cACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctg         >  1:10505573/1‑151 (MQ=255)
                                                                                                                                            ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct       <  2:3959309/151‑1 (MQ=255)
                                                                                                                                            ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct       >  2:2637505/1‑151 (MQ=255)
                                                                                                                                             cAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgctg      >  1:10799931/1‑151 (MQ=255)
                                                                                                                                              aGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTgg     >  1:5787314/1‑151 (MQ=255)
                                                                                                                                               gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGgc    <  1:548408/151‑1 (MQ=255)
                                                                                                                                               gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGgc    <  2:10588865/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTGGCGATCATGCTCGGGCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGC  >  NC_008463/2651191‑2651486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑