Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,651,357 A→G N74D (AAC→GAC)  PA14_RS12480 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,651,3570AG100.0% 2004.7 / NA 509N74D (AAC→GAC) PA14_RS12480MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (237/272);  total (237/272)

Only 100 of 509 total aligned reads displayed.

GCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTACG  >  NC_008463/2651209‑2651504
                                                                                                                                                    |                                                                                                                                                   
 cccACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGAcc                                                                                                                                                  <  2:10205198/151‑1 (MQ=255)
   cACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGt                                                                                                                                                <  2:10433203/151‑1 (MQ=255)
    aCCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGtt                                                                                                                                               >  1:7156282/1‑151 (MQ=255)
    aCCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGtt                                                                                                                                               >  2:1278000/1‑151 (MQ=255)
     ccGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTg                                                                                                                                              >  2:6099862/1‑151 (MQ=255)
       ggCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTggg                                                                                                                                            <  2:5119938/151‑1 (MQ=255)
        gCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgggg                                                                                                                                           <  1:6354651/151‑1 (MQ=255)
        gCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTgggg                                                                                                                                           <  2:5696520/151‑1 (MQ=255)
         cATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgc                                                                                                                                          >  2:8867697/1‑151 (MQ=255)
          aTCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcg                                                                                                                                         <  2:560021/151‑1 (MQ=255)
            ccTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGgcgcg                                                                                                                                       <  2:2765747/151‑1 (MQ=255)
                  gAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGcggcgg                                                                                                                                 >  1:9302902/1‑151 (MQ=255)
                  gAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGcggcgg                                                                                                                                 <  2:689600/151‑1 (MQ=255)
                       ccTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAt                                                                                                                            >  2:5435200/1‑151 (MQ=255)
                         ttCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGc                                                                                                                          <  1:6891318/151‑1 (MQ=255)
                            aGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGCCCATGCTCg                                                                                                                       >  2:4006182/1‑151 (MQ=255)
                              tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCggg                                                                                                                     >  1:8075308/1‑151 (MQ=255)
                              tCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCggg                                                                                                                     <  2:969344/151‑1 (MQ=255)
                                tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCt                                                                                                                   <  2:10737447/151‑1 (MQ=255)
                                tGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCt                                                                                                                   <  1:2739527/151‑1 (MQ=255)
                                  ttCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGa                                                                                                                 <  1:4384575/151‑1 (MQ=255)
                                    cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                                               <  2:8174367/151‑1 (MQ=255)
                                    cATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGAcc                                                                                                               <  1:4684806/151‑1 (MQ=255)
                                         cACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGc                                                                                                          >  1:4199731/1‑151 (MQ=255)
                                            cGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCt                                                                                                       <  2:8374313/151‑1 (MQ=255)
                                             gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                                      <  1:3071296/151‑1 (MQ=255)
                                             gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                                      >  1:7447980/1‑151 (MQ=255)
                                             gATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTg                                                                                                      >  1:10674967/1‑151 (MQ=255)
                                              aTGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGt                                                                                                     <  1:2281209/151‑1 (MQ=255)
                                                gAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcg                                                                                                   >  2:4939301/1‑151 (MQ=255)
                                                  gcgcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGATGGGGCGCGCCGCGGAATAAGCTCGGGCTGACCGCGGCACTggagct                                                                                                 >  1:6802735/1‑146 (MQ=255)
                                                    gcCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTcgctgc                                                                                               <  2:3328557/151‑1 (MQ=255)
                                                         ggaCGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGGcgctgccgctg                                                                                          >  1:8505164/3‑151 (MQ=255)
                                                            cGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTc                                                                                       <  1:3794806/151‑1 (MQ=255)
                                                             gCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCg                                                                                      >  1:2531162/1‑151 (MQ=255)
                                                              ccTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCgg                                                                                     <  1:6170542/151‑1 (MQ=255)
                                                               cTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGc                                                                                    <  1:3260420/151‑1 (MQ=255)
                                                                   gACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGCTCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTgg                                                                                <  2:6463126/151‑1 (MQ=255)
                                                                    aCCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggc                                                                               >  1:9108960/1‑151 (MQ=255)
                                                                     ccGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcg                                                                              <  1:5602233/151‑1 (MQ=255)
                                                                     ccGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTggcg                                                                              >  2:163068/1‑151 (MQ=255)
                                                                        cgcCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGgcggcg                                                                           >  2:2006399/1‑151 (MQ=255)
                                                                          ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGTCCGCGGCCCTGACGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                                         >  2:9031852/1‑151 (MQ=255)
                                                                          ccGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGt                                                                         >  2:2967427/1‑151 (MQ=255)
                                                                           cGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTg                                                                        <  1:9694380/151‑1 (MQ=255)
                                                                            gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCTGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGTCAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGa                                                                       <  2:1824433/151‑1 (MQ=255)
                                                                            gAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGa                                                                       >  2:5961329/1‑151 (MQ=255)
                                                                              ggTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGGGAAt                                                                     >  1:8346671/1‑151 (MQ=255)
                                                                              ggTGTCGGTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAAt                                                                     <  2:7465391/151‑1 (MQ=255)
                                                                                tgtCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGc                                                                   >  2:9227966/1‑151 (MQ=255)
                                                                                   cGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGt                                                                <  1:4964993/151‑1 (MQ=255)
                                                                                     ttGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCt                                                              >  2:3192373/1‑151 (MQ=255)
                                                                                        cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGACTGCCGTCTGGc                                                           >  2:7215249/1‑151 (MQ=255)
                                                                                        cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGc                                                           >  2:6581977/1‑151 (MQ=255)
                                                                                        cTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGGGAATGCCGTCTGGc                                                           <  2:1378914/151‑1 (MQ=255)
                                                                                         tGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCa                                                          >  2:2438085/1‑151 (MQ=255)
                                                                                         tGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCa                                                          >  1:8877535/1‑151 (MQ=255)
                                                                                          ggTGACGGTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCGGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGTCCGCGGCCCTGTAGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCaa                                                         <  1:243080/151‑1 (MQ=255)
                                                                                           gTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAc                                                        <  1:1791085/151‑1 (MQ=255)
                                                                                            tGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAAct                                                       <  1:10911389/151‑1 (MQ=255)
                                                                                             gACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAActc                                                      <  1:10075932/151‑1 (MQ=255)
                                                                                               cGCTGTTCGGCCTGGCGGTCGCCATCACCCGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACCATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCGCGGCCTGGCGGCGGGGAATGCCGTCTGGCAACTCTa                                                    <  2:7808474/151‑1 (MQ=255)
                                                                                                gCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAc                                                   >  2:9502368/1‑151 (MQ=255)
                                                                                                 cTGTTCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTAcg                                                  >  1:7780825/1‑151 (MQ=255)
                                                                                                    ttCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACgcgc                                               <  2:4766631/151‑1 (MQ=255)
                                                                                                     tCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                                              <  2:4277110/151‑1 (MQ=255)
                                                                                                     tCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                                              <  1:10157510/151‑1 (MQ=255)
                                                                                                     tCGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCt                                              <  1:6646619/151‑1 (MQ=255)
                                                                                                      cGGACTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTg                                             <  2:4439432/151‑1 (MQ=255)
                                                                                                         aCTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTc                                          <  2:3930997/151‑1 (MQ=255)
                                                                                                         aCTGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTc                                          <  1:1877303/151‑1 (MQ=255)
                                                                                                           tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                                        >  2:6810172/1‑151 (MQ=255)
                                                                                                           tGGCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCct                                        >  2:7905507/1‑151 (MQ=255)
                                                                                                             gCGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgc                                      >  2:10261154/1‑151 (MQ=255)
                                                                                                              cGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                                     <  1:10392910/151‑1 (MQ=255)
                                                                                                              cGGTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgct                                     <  2:10122808/151‑1 (MQ=255)
                                                                                                               ggTCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCctgctg                                    <  1:7239622/151‑1 (MQ=255)
                                                                                                                 tCGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAt                                  >  1:10558085/1‑151 (MQ=255)
                                                                                                                  cGCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATc                                 <  1:3944024/151‑1 (MQ=255)
                                                                                                                   gCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCg                                >  2:7605969/1‑151 (MQ=255)
                                                                                                                   gCCATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAAGGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGAtag                                <  1:8828215/151‑3 (MQ=255)
                                                                                                                     cATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc                              >  2:5447041/1‑151 (MQ=255)
                                                                                                                     cATCACCAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGc                              >  2:4379561/1‑151 (MQ=255)
                                                                                                                          ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct                         <  2:6353359/151‑1 (MQ=255)
                                                                                                                          ccAGCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCctgct                         >  2:2637505/1‑151 (MQ=255)
                                                                                                                             gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGgc                      <  2:9831500/151‑1 (MQ=255)
                                                                                                                             gCCCGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGgc                      >  1:7731719/1‑151 (MQ=255)
                                                                                                                               ccGCTCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTTACAGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACACTACGCGCTGTTCATGCTGATCGGCCTGCAGgcgc                    >  1:5414799/1‑151 (MQ=255)
                                                                                                                                 gctCAAGGGCTGGCTGCTCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCa                  <  2:1773946/149‑1 (MQ=255)
                                                                                                                                  ctCAAGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAg                 <  1:5859909/150‑1 (MQ=255)
                                                                                                                                     aaGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCa              >  1:3351500/1‑151 (MQ=255)
                                                                                                                                      aGGGCTGGCTGATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCaa             >  1:120917/1‑151 (MQ=255)
                                                                                                                                         gctggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCt          <  1:6487801/151‑1 (MQ=255)
                                                                                                                                         gctggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCt          >  1:9523491/1‑151 (MQ=255)
                                                                                                                                          ctggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTg         >  1:10839437/1‑151 (MQ=255)
                                                                                                                                          ctggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTg         <  1:9996761/151‑1 (MQ=255)
                                                                                                                                           tggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGGTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGc        >  1:1624457/1‑151 (MQ=255)
                                                                                                                                            ggctgATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGcc       <  2:1533941/151‑1 (MQ=255)
                                                                                                                                                gATCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTAc   >  2:8037467/1‑151 (MQ=255)
                                                                                                                                                 aTCGACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTACg  >  1:1353560/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCACCGGCATCCTGCTGAACACCTTCAGTCTGTTCATCGCACCGATGAGCGCCGAGTACGCCTGGGACCGCGCCGAGGTGTCGTTGCTGGTGACGCTGTTCGGACTGGCGGTCGCCATCACCAGCCCGTTCAAGGGCTGGCTGATCAACCGTTGGGGCGCGCGGCGGACAATGCTCGGGCTGACCGCGGCCCTGTCGCTGCCGCTGCTCGGCCTGGCGGCGGTGAATGCCGTCTGGCAACTCTACGCGCTGTTCCTGCTGATCGGCCTGCTGGCGCCAGGCAACCTGCCTTACG  >  NC_008463/2651209‑2651504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑