BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,673,534	3 bp→CTC	intergenic (+83/+13)	fabI → / ← PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,673,534	0	T	C	100.0%	1535.9 / NA	397	intergenic (+83/+15)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (228/169); total (228/169)
*	NC_008463	3,673,535	0	C	T	95.4%	1655.7 / 38.6	416	intergenic (+84/+14)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (13/6); new base T (227/167); total (241/175)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01
*	NC_008463	3,673,536	0	T	C	95.4%	1507.7 / 47.9	416	intergenic (+85/+13)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (13/6); new base C (228/169); total (241/175)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 416 total aligned reads displayed.

ATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTC  >  NC_008463/3673392‑3673680

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 tCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGAACACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtct                                                                                                                                           <  1:6433839/151‑1 (MQ=255)

  ccTCTACGTCGATGGCGGCTTCAACACTCCCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAAGAACCGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtctc                                                                                                                                          <  2:4428342/151‑1 (MQ=255)

  ccTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtctc                                                                                                                                          <  2:4345882/151‑1 (MQ=255)

   ctctACGTCGATGGCGGCTTCAACCCTACCCCCAGGGGCCCGCTGTACGACGACTTATCACCCCGCTAATGAAAAATGGCCGCTACGAGCGGCCTTTTTCTTGGGCGTAATAACCAACCGCCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCg                                                                                                                                         <  1:9830742/151‑1 (MQ=255)

     ctACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCGCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGt                                                                                                                                       >  2:1695818/1‑151 (MQ=255)

     ctACGTCGATGGCGGCTACAACACTCCCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGt                                                                                                                                       <  1:5320182/151‑1 (MQ=255)

      tACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtc                                                                                                                                      >  1:1906027/1‑151 (MQ=255)

      caCGCCGATGGCGGCTACAACACTACCGCCATGGGCCCGCTGGACTACGACTGATCACCCCGCACATGAAAAAGGGCCGCTACGAGCGGCCTTTTTCATGGGCGCAAAAACCAAAACGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtc                                                                                                                                      <  1:7620628/150‑1 (MQ=255)

       aCGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtgg                                                                                                                                     >  1:10351104/1‑149 (MQ=255)

         gTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCa                                                                                                                                   >  1:5930496/1‑151 (MQ=255)

           cGATGGCGGCTTCAACACAACCGCCAGGGGCCCGCTGGACGACGACTGATCACCCCGCACATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAACAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGt                                                                                                                                 <  1:5642745/151‑1 (MQ=255)

            gATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTa                                                                                                                                <  2:9339642/151‑1 (MQ=255)

               ggcggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTAttt                                                                                                                             >  1:10456108/1‑151 (MQ=255)

                  ggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGACTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTtctc                                                                                                                          >  2:5320647/1‑151 (MQ=255)

                     ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCTAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                       >  1:3205665/1‑151 (MQ=255)

                     ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAATGGCCGCGGAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCTGTAGTTCTCGAc                                                                                                                       >  2:10001561/1‑151 (MQ=255)

                     ttCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                       >  2:8866533/1‑151 (MQ=255)

                        aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                                    >  1:10832868/1‑151 (MQ=255)

                                 gCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGACTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                                           >  1:2808104/1‑151 (MQ=255)

                                  ccATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTcc                                                                                                          <  2:3137476/151‑1 (MQ=255)

                                     tGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGc                                                                                                       >  2:678157/1‑151 (MQ=255)

                                       ggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCaa                                                                                                     <  2:8473930/151‑1 (MQ=255)

                                       ggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCaa                                                                                                     >  1:4338609/1‑151 (MQ=255)

                                        gaccGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCAGGGGCGGAATAATGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCTGTATTTCTCGACTGCCGACTTGTCCTGCAAc                                                                                                    >  2:3040796/3‑151 (MQ=255)

                                        gCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAAc                                                                                                    >  1:8935079/1‑151 (MQ=255)

                                         cccGGTGGACGACTCCTTATCACCCCGCTCATTAAAATTGGCGGCTTCGAGCGGCTTTTTTCTTGGGCGTAATACAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACt                                                                                                   <  1:9326363/151‑1 (MQ=255)

                                           cGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAAGAAAGAAAAGGCCGCGTCAGCGGCCATTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                                 <  2:6311136/151‑1 (MQ=255)

                                            gCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAc                                                                                                <  1:7089215/151‑1 (MQ=255)

                                            gCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAc                                                                                                <  2:5617906/151‑1 (MQ=255)

                                             cTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTAGGTCTCAGTATTTCTCGACTTCCGCATTGTCCTGCAACTGAcg                                                                                               >  2:1470879/1‑151 (MQ=255)

                                             cTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTCCGAGCGGCCTTTTTCATGGGCGGAATAAAGCAAAGGCCGCGTAAGCGCCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcg                                                                                               <  1:1290024/151‑1 (MQ=255)

                                              tGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcgg                                                                                              <  1:911883/151‑1 (MQ=255)

                                              tGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcgg                                                                                              >  2:355389/1‑151 (MQ=255)

                                                          gATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGgcggcg                                                                                  <  2:7573098/151‑1 (MQ=255)

                                                            tCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGaa                                                                                >  1:11001673/1‑151 (MQ=255)

                                                               ccccGCTCATGAACAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTc                                                                             <  2:3908353/151‑1 (MQ=255)

                                                                cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCt                                                                            >  2:4932714/1‑151 (MQ=255)

                                                                cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTATCCGGGGCGGACTAAAGAAAAGGCCGCGTAAGCGGCCGTTTCTC‑TGCAGCTCGGGCTCAGTATTTCTCTACGTCGGCCGTGTCCTGCAACTGACGGCGGTAGGCGGCGAAGTCt                                                                            >  2:7342460/1‑151 (MQ=255)

                                                                   gCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGcc                                                                         <  2:1192837/151‑1 (MQ=255)

                                                                    cTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTACTC‑GGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCt                                                                        <  2:8319177/151‑1 (MQ=255)

                                                                     tCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTg                                                                       <  2:2496801/151‑1 (MQ=255)

                                                                     tCATGAAAAAGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTg                                                                       <  1:3030847/151‑1 (MQ=255)

                                                                      cATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGt                                                                      <  2:6028383/151‑1 (MQ=255)

                                                                      cATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGt                                                                      >  2:4056446/1‑151 (MQ=255)

                                                                       aTGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTc                                                                     >  1:8402190/1‑151 (MQ=255)

                                                                        tGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTcc                                                                    <  2:8089369/151‑1 (MQ=255)

                                                                         gAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCg                                                                   >  1:2954357/1‑151 (MQ=255)

                                                                            aaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTg                                                                >  1:4986922/1‑151 (MQ=255)

                                                                                     cTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                                       >  1:2556474/1‑151 (MQ=255)

                                                                                     cTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                                       >  1:8102662/1‑151 (MQ=255)

                                                                                        cGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAgg                                                    >  2:6510557/1‑151 (MQ=255)

                                                                                        cGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAgg                                                    <  1:6142797/151‑1 (MQ=255)

                                                                                         gAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                   >  1:3983939/1‑151 (MQ=255)

                                                                                         gAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                   >  2:2274237/1‑151 (MQ=255)

                                                                                          aGCGGCCTTTTTCATGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                  <  2:10301832/151‑1 (MQ=255)

                                                                                          aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                  >  1:2171301/1‑151 (MQ=255)

                                                                                          aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                  >  2:10159001/1‑151 (MQ=255)

                                                                                           gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAg                                                 >  1:7525410/1‑151 (MQ=255)

                                                                                            cGGCCTTTTTCATGGGCGGAATAAAGAAATGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATGTCTCGACTTCCGCCGTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACgc                                                >  2:5093143/1‑151 (MQ=255)

                                                                                              gCCTTTTTCATGGGCGGAATCAAGAACAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcgg                                              <  2:4362476/151‑1 (MQ=255)

                                                                                               ccTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggc                                             >  1:10029300/1‑151 (MQ=255)

                                                                                                cTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcggcg                                            >  1:923056/1‑151 (MQ=255)

                                                                                                  ttttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGt                                          <  1:9059096/151‑1 (MQ=255)

                                                                                                   tttCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTa                                         >  1:10258015/1‑151 (MQ=255)

                                                                                                      cATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACAt                                      <  1:4695467/151‑1 (MQ=255)

                                                                                                         gggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGc                                   >  2:7654759/1‑151 (MQ=255)

                                                                                                          ggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGcc                                  >  2:2162933/1‑151 (MQ=255)

                                                                                                          ggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGcc                                  >  1:9605607/1‑151 (MQ=255)

                                                                                                           gCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCt                                 >  1:8292056/1‑151 (MQ=255)

                                                                                                           gCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCt                                 <  2:3805530/151‑1 (MQ=255)

                                                                                                            cGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                                >  1:1794408/1‑151 (MQ=255)

                                                                                                              gAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtct                              >  1:9715701/1‑151 (MQ=255)

                                                                                                               aaTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                             >  1:3395202/1‑151 (MQ=255)

                                                                                                               aaTAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTtctc                             >  1:10637800/1‑151 (MQ=255)

                                                                                                                aTAACGAAAAGGCCGCGTACGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcg                            <  1:6636209/151‑1 (MQ=255)

                                                                                                                 tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                           <  2:10984378/151‑1 (MQ=255)

                                                                                                                  aacgAAACGGCCGCGTAAGCGGCCTTTTCTC‑CGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGCCTTCtcgtc                          <  2:2900193/148‑1 (MQ=255)

                                                                                                                    aGAAAAGGCCGCGTATGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGACGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt                        >  1:7803427/1‑151 (MQ=255)

                                                                                                                        aaGGCAGCGTAAGCGGCCTTATCTC‑TGCATATCGGTCTCAGTATTTCTCGAATACCGCCTTGTCCTGCAACAGACGTCGGAAGGCGGCGTAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCATTCTCGTCGTCGga                    >  2:10433555/1‑151 (MQ=255)

                                                                                                                         aGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgag                   <  2:10871642/151‑1 (MQ=255)

                                                                                                                         aGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgag                   >  1:5090283/1‑151 (MQ=255)

                                                                                                                          ggCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga                  >  1:1416890/1‑151 (MQ=255)

                                                                                                                             cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg               >  1:6973165/1‑151 (MQ=255)

                                                                                                                             cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg               <  2:5348656/151‑1 (MQ=255)

                                                                                                                             cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAg               >  1:10559697/1‑151 (MQ=255)

                                                                                                                              gCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGc              >  1:2327575/1‑151 (MQ=255)

                                                                                                                               cgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcc             >  2:1986290/2‑151 (MQ=255)

                                                                                                                               cgTAAGCGGCCTTTTCAC‑TGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcc             <  2:878497/150‑1 (MQ=255)

                                                                                                                                gTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct            >  2:9463364/1‑151 (MQ=255)

                                                                                                                                 tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcctc           >  2:914707/1‑151 (MQ=255)

                                                                                                                                 tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTCTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATCGcctc           >  2:9638874/1‑151 (MQ=255)

                                                                                                                                     cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTAATTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc       >  2:3335578/1‑151 (MQ=255)

                                                                                                                                      ggCCTTTTCTC‑TGCCTCACGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCg      <  1:3698948/151‑1 (MQ=255)

                                                                                                                                       gCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTCACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCgg     <  1:1611599/151‑1 (MQ=255)

                                                                                                                                         ccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGc    >  1:4181507/3‑151 (MQ=255)

                                                                                                                                         ccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGc    >  2:5815602/3‑151 (MQ=255)

                                                                                                                                         ccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGc    >  2:10628072/3‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  >  1:1657353/1‑151 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  <  2:714552/151‑1 (MQ=255)

                                                                                                                                           ttttCTCTGCATCTCGGTCTCAGTAGTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTc  <  1:10536572/151‑1 (MQ=255)

|||

ATCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCTC  >  NC_008463/3673392‑3673680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`