Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463888,0380CT99.3% 2326.4 / NA 560P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (301/254);  minor base A (2/2);  total (304/256)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463888,0390TG99.6% 2191.1 / NA 560P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (304/254);  total (304/256)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 560 total aligned reads displayed.

GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183
                                                                                                                                                 ||                                                                                                                                                
gAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAAt                                                                                                                                              >  1:10051882/1‑151 (MQ=255)
gAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAAt                                                                                                                                              <  1:4486380/151‑1 (MQ=255)
    aTCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCt                                                                                                                                          >  2:3944329/1‑151 (MQ=255)
     tctcTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTg                                                                                                                                         >  1:7443164/1‑151 (MQ=255)
          aCTTCCGCCTCCGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGACCGCAGCGACCGGCTGGCGCTGCTCCGGCGCATCCACGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACACGGCGAGGCAGCCCAGGAATGCCTGCTCgg                                                                                                                                    <  2:6388697/151‑1 (MQ=255)
           cTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGc                                                                                                                                   >  2:8265155/1‑151 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  <  2:7088814/151‑1 (MQ=255)
             tCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGATCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTa                                                                                                                                 >  1:6737693/1‑151 (MQ=255)
             tCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTa                                                                                                                                 >  1:6665039/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              >  1:5156068/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              <  2:3032931/151‑1 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              >  1:3160761/1‑151 (MQ=255)
                  cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                            >  2:9006093/1‑151 (MQ=255)
                  cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                            <  2:4567386/151‑1 (MQ=255)
                   tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                           <  1:1616977/151‑1 (MQ=255)
                   tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                           <  2:10508751/151‑1 (MQ=255)
                    cggcggCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTgg                                                                                                                          >  1:4595674/1‑151 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         <  2:4795542/151‑1 (MQ=255)
                       cggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCg                                                                                                                       <  1:1916409/151‑1 (MQ=255)
                        ggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCgg                                                                                                                      <  1:8399468/151‑1 (MQ=255)
                        ggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCgg                                                                                                                      <  1:10694292/151‑1 (MQ=255)
                          cgcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGc                                                                                                                    <  2:9208639/151‑1 (MQ=255)
                            cccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCaa                                                                                                                  >  1:6149288/1‑151 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAt                                                                                                                 <  2:6096239/151‑1 (MQ=255)
                              cGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATg                                                                                                                >  2:8758012/1‑151 (MQ=255)
                               gCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCGGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGt                                                                                                               <  1:3519994/151‑1 (MQ=255)
                               gCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGt                                                                                                               >  1:826493/1‑151 (MQ=255)
                                  tCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCg                                                                                                            <  2:2827603/151‑1 (MQ=255)
                                   cGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGc                                                                                                           >  1:8646967/1‑151 (MQ=255)
                                         gccgccGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTg                                                                                                     <  1:10185794/151‑1 (MQ=255)
                                           cgccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCg                                                                                                   >  1:5397562/1‑151 (MQ=255)
                                             ccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGc                                                                                                 <  2:569826/151‑1 (MQ=255)
                                                cTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtg                                                                                              >  2:3637697/1‑151 (MQ=255)
                                                 tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgt                                                                                             <  1:2230861/151‑1 (MQ=255)
                                                  aCGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgtg                                                                                            >  2:9433546/1‑151 (MQ=255)
                                                  aCGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgtg                                                                                            <  1:1270409/151‑1 (MQ=255)
                                                        aCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgcgc                                                                                      >  2:529711/1‑151 (MQ=255)
                                                         cGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCt                                                                                     >  1:6395368/1‑151 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCtc                                                                                    <  1:9392633/151‑2 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    >  1:8065071/1‑151 (MQ=255)
                                                            aGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACg                                                                                  <  2:1982072/151‑1 (MQ=255)
                                                              cGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGcc                                                                                <  1:4826723/151‑1 (MQ=255)
                                                                aCCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTg                                                                              <  1:3396129/151‑1 (MQ=255)
                                                                  cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcg                                                                            >  2:4180775/1‑151 (MQ=255)
                                                                  cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcg                                                                            <  2:5518195/151‑1 (MQ=255)
                                                                  cGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAGGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcg                                                                            <  2:1465668/151‑1 (MQ=255)
                                                                    gCTGGCGCTGATCCGGCTCATCCTCGACGAGGAATCCGCGCTCTGCGGCGTACGCATCGAACTCGGCGAGGCAGGACTTGAATGTCTGCTCGGCTATCGCTGGCCGGGCAATGTTCGCCTGTTGCGTCCTGGGTTGCGCTCCGCCTgcgcg                                                                          <  2:2241811/151‑1 (MQ=255)
                                                                     cTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcgcgc                                                                         >  1:7538107/1‑151 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        <  2:6005287/151‑1 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        >  2:4038892/1‑151 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        >  1:1150725/1‑151 (MQ=255)
                                                                         cgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgtc                                                                     <  1:9296426/151‑2 (MQ=255)
                                                                          gcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgc                                                                    <  2:7753251/151‑1 (MQ=255)
                                                                           cTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcg                                                                   <  2:3338909/151‑1 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 >  2:717864/1‑151 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 >  2:10291944/1‑151 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                >  2:7979812/1‑151 (MQ=255)
                                                                                ccGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                              <  1:7212546/151‑1 (MQ=255)
                                                                                 cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggcg                                                             <  1:9670286/151‑1 (MQ=255)
                                                                                 cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggcg                                                             >  2:10823608/1‑151 (MQ=255)
                                                                                 cGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggcg                                                             <  1:10444484/151‑1 (MQ=255)
                                                                                  ggCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGgcgc                                                            >  2:6251471/1‑151 (MQ=255)
                                                                                     gcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAc                                                         >  1:5546704/1‑151 (MQ=255)
                                                                                     gcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAc                                                         >  1:6556251/1‑151 (MQ=255)
                                                                                       aTCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAccc                                                       <  2:8374591/151‑1 (MQ=255)
                                                                                       aTCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAccc                                                       <  2:11105259/151‑1 (MQ=255)
                                                                                          cTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGc                                                    >  1:1726925/1‑151 (MQ=255)
                                                                                           tCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCa                                                   <  1:10917890/151‑1 (MQ=255)
                                                                                               cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTc                                               <  1:3985663/151‑1 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              >  2:1265082/1‑151 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              <  2:3797671/151‑1 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCGATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTTCGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              <  2:1866604/151‑1 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          >  1:4208400/1‑151 (MQ=255)
                                                                                                     aaCCGCGCACTGCGGCGTACGCATCGAACGCGGCGAGGCAGCCAGGGACTGCCTGCTCGGCGATCGCTGGCCGGGCAAGGTCCGCCAGTTGCGGCCGGTGTTGCGCTACGCCTGCGCGCTGTGCGGGGGCGCCACCCTGCAAGTCGCCGAc                                         >  2:4264664/1‑151 (MQ=255)
                                                                                                        cgcgCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGTAACTCGCCGACCtt                                      <  1:2713552/151‑1 (MQ=255)
                                                                                                              cTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCa                                <  2:7588123/151‑1 (MQ=255)
                                                                                                                 cggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCagag                             >  1:6981872/1‑151 (MQ=255)
                                                                                                                   gcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCt                           >  1:8985975/1‑151 (MQ=255)
                                                                                                                    cgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTg                          <  1:2654545/151‑1 (MQ=255)
                                                                                                                    cgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTg                          <  1:3670146/151‑1 (MQ=255)
                                                                                                                    cgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTg                          <  2:2590852/151‑1 (MQ=255)
                                                                                                                     gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  2:7549420/1‑151 (MQ=255)
                                                                                                                          cATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCCCCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGc                    <  1:5751178/151‑1 (MQ=255)
                                                                                                                          cATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGc                    >  2:6987869/1‑151 (MQ=255)
                                                                                                                            tCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGa                  >  2:5378425/1‑151 (MQ=255)
                                                                                                                            tCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGa                  >  1:9939371/1‑151 (MQ=255)
                                                                                                                              gAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAg                >  1:6792968/1‑151 (MQ=255)
                                                                                                                               aaCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAgg               <  2:10033325/151‑1 (MQ=255)
                                                                                                                               aaCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAgg               >  2:4908827/1‑151 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              <  2:5800021/151‑1 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              >  1:7809352/1‑151 (MQ=255)
                                                                                                                                  tCGGCGAGGCCGCCCTGGAATGCCTGCTCGGGTCTCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTCCGCCTGCGCGCTGGGCGGCGGCGCCCCCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCg            >  2:8606103/1‑151 (MQ=255)
                                                                                                                                   cGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGTGCTGCGTGGCGAAGGCCgg           >  2:9761949/1‑151 (MQ=255)
                                                                                                                                    ggCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGa          >  2:3221694/1‑151 (MQ=255)
                                                                                                                                     gCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGac         <  1:2469523/151‑1 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacac       >  2:1624498/1‑151 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacac       >  2:6586955/1‑151 (MQ=255)
                                                                                                                                        aGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  2:242840/1‑132 (MQ=255)
                                                                                                                                           cAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGc   >  1:5966116/1‑151 (MQ=255)
                                                                                                                                            aGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGcc  <  2:6681466/151‑1 (MQ=255)
                                                                                                                                                 ||                                                                                                                                                
GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑