Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,807,266 G→C V137L (GTG→CTG)  PA14_RS13175 → leucine‑rich repeat‑containing protein kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,807,2660GC99.8% 1704.5 / NA 429V137L (GTG→CTG) PA14_RS13175leucine‑rich repeat‑containing protein kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (229/199);  minor base A (1/0);  total (230/199)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413
                                                                                                                                                    |                                                                                                                                                   
 gCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGa                                                                                                                                                  >  2:5809642/1‑151 (MQ=255)
    gACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCa                                                                                                                                               >  2:9241461/1‑151 (MQ=255)
    gACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCa                                                                                                                                               >  2:8933914/1‑151 (MQ=255)
      cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATg                                                                                                                                             <  1:182551/151‑1 (MQ=255)
        cccTGGAGGTGCTCAACCTCAGCGGCAACGGCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCCTCGTCTCCCGGTGCTGTTCTGTTCCGCCAACGAGTTCGCCGAAGTGCCGGCGGCGGTGGGCGTGTGTCCGGGGCTGTGCAgggg                                                                                                                                           >  2:6101980/1‑150 (MQ=255)
         ccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTc                                                                                                                                          >  2:421997/1‑151 (MQ=255)
         ccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTc                                                                                                                                          <  1:3649962/151‑1 (MQ=255)
          cTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCg                                                                                                                                         <  2:7685175/151‑1 (MQ=255)
           tggtggTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCgg                                                                                                                                        <  2:684870/151‑1 (MQ=255)
            ggAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGc                                                                                                                                       >  2:1765149/1‑151 (MQ=255)
            ggAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGc                                                                                                                                       >  1:42099/1‑151 (MQ=255)
               ggTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTc                                                                                                                                    <  2:8491262/151‑1 (MQ=255)
                gTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCa                                                                                                                                   <  2:2850271/151‑1 (MQ=255)
                gTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCa                                                                                                                                   <  1:3368727/151‑1 (MQ=255)
                   cTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGt                                                                                                                                <  2:5662587/151‑1 (MQ=255)
                    tCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                               <  2:5480769/151‑1 (MQ=255)
                     cAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTcc                                                                                                                              >  1:3058762/1‑151 (MQ=255)
                     cAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTcc                                                                                                                              >  2:5471798/1‑151 (MQ=255)
                      aaCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCa                                                                                                                             >  1:2686751/1‑151 (MQ=255)
                      aaCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGGCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCa                                                                                                                             >  2:9783522/1‑151 (MQ=255)
                        ccTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAAc                                                                                                                           >  2:3179606/1‑151 (MQ=255)
                         cTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAAcc                                                                                                                          >  1:2192378/1‑151 (MQ=255)
                            aGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCa                                                                                                                       >  2:7383045/1‑151 (MQ=255)
                             gCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCAt                                                                                                                      <  1:2497450/151‑1 (MQ=255)
                              cGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATc                                                                                                                     <  2:6150989/151‑1 (MQ=255)
                                 cAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAg                                                                                                                  >  1:5544674/1‑151 (MQ=255)
                                  aaCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGc                                                                                                                 >  1:4433010/1‑151 (MQ=255)
                                  aaCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGc                                                                                                                 >  2:3913513/1‑151 (MQ=255)
                                   aCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCg                                                                                                                <  2:6958982/151‑1 (MQ=255)
                                      cccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGt                                                                                                             <  2:6565574/151‑1 (MQ=255)
                                        cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgc                                                                                                           <  2:1111336/151‑1 (MQ=255)
                                        cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgc                                                                                                           <  2:10026502/151‑1 (MQ=255)
                                         tGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTGCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgcc                                                                                                          >  1:7146450/1‑151 (MQ=255)
                                          ggACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccg                                                                                                         <  1:3464581/151‑1 (MQ=255)
                                          ggACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccg                                                                                                         <  1:8844991/151‑1 (MQ=255)
                                           gACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccgg                                                                                                        >  1:4759150/1‑151 (MQ=255)
                                             cAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggcc                                                                                                      >  1:10295400/1‑151 (MQ=255)
                                             cAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggcc                                                                                                      <  1:1070316/151‑1 (MQ=255)
                                              aGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                     >  1:6547656/1‑151 (MQ=255)
                                              aGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                     >  1:10549844/1‑151 (MQ=255)
                                               gCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCcgc                                                                                                    >  1:988603/1‑151 (MQ=255)
                                                  tGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGc                                                                                                 >  1:8901250/1‑151 (MQ=255)
                                                  tGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGc                                                                                                 <  1:592825/151‑1 (MQ=255)
                                                     cGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGGTCCGCCAACGTCTTCGCCTTATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTGCAACCGCATCGAGCGGGTGACGGCCGCGGCCCt                                                                                              <  2:9422116/151‑1 (MQ=255)
                                                      ggACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTg                                                                                             <  1:2401059/151‑1 (MQ=255)
                                                      ggACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTg                                                                                             >  1:6824/1‑151 (MQ=255)
                                                        acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgcc                                                                                           >  1:5383424/1‑151 (MQ=255)
                                                          gacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                         >  1:10063506/1‑151 (MQ=255)
                                                          gacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                         <  2:7602978/151‑1 (MQ=255)
                                                              tCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGc                                                                                     <  2:9015079/151‑1 (MQ=255)
                                                                   cggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgc                                                                                <  2:8148074/151‑1 (MQ=255)
                                                                   cggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgc                                                                                >  1:4061924/1‑151 (MQ=255)
                                                                    ggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgcg                                                                               >  2:5071106/1‑151 (MQ=255)
                                                                     gcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgcgc                                                                              >  1:7278161/1‑151 (MQ=255)
                                                                      cTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgct                                                                             >  2:8780580/1‑151 (MQ=255)
                                                                        gCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctgg                                                                           <  2:2597568/151‑1 (MQ=255)
                                                                             gTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGAt                                                                      >  2:9692204/1‑151 (MQ=255)
                                                                              tctcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATc                                                                     >  2:8029912/1‑151 (MQ=255)
                                                                               ctcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATcc                                                                    >  1:6261515/1‑151 (MQ=255)
                                                                                 cAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTc                                                                  >  2:4560734/1‑151 (MQ=255)
                                                                                           ttctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAcc                                                        <  1:1411104/151‑1 (MQ=255)
                                                                                           ttctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAcc                                                        <  1:9704213/151‑1 (MQ=255)
                                                                                           ttctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAcc                                                        >  1:9560189/1‑151 (MQ=255)
                                                                                            tctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCg                                                       <  2:3444090/151‑1 (MQ=255)
                                                                                             ctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCgg                                                      >  1:6869555/1‑151 (MQ=255)
                                                                                                   cGCCCACGACTTAGCCGAATTTCCGGCGGCGGTTGGCGACAGTCCGGCGCAGAGCAATGTCGGCTTAAAGTCCAAACCCCTCGAGCGGGTGCCCGCCCCGGCCCAGCCGCCGGCGTTGCGCTGGCTGATCCACACCCACAACCCGATTGcc                                                >  2:8579686/1‑151 (MQ=255)
                                                                                                   cGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGcc                                                <  1:2092919/151‑1 (MQ=255)
                                                                                                   cGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGcc                                                <  1:7975945/151‑1 (MQ=255)
                                                                                                    gCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCa                                               >  1:4640396/1‑151 (MQ=255)
                                                                                                    gCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCa                                               >  2:2768308/1‑151 (MQ=255)
                                                                                                      cAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACg                                             >  1:84258/1‑151 (MQ=255)
                                                                                                      cAACGACTTCGCCGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCTGCTTCTAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAGTGCCACg                                             <  2:2345481/151‑1 (MQ=255)
                                                                                                        aCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCt                                           <  1:6474652/151‑1 (MQ=255)
                                                                                                          gACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                         >  1:5062737/1‑151 (MQ=255)
                                                                                                           aCTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGcc                                        >  2:3300724/1‑151 (MQ=255)
                                                                                                            ctacGCCGTATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGccc                                       <  2:2491425/148‑1 (MQ=255)
                                                                                                               cGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAg                                    >  2:3994667/1‑151 (MQ=255)
                                                                                                                gCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAgg                                   <  1:9143386/151‑1 (MQ=255)
                                                                                                                 ccGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAGTGCCACGCTGCCCGAGGa                                  >  2:10390278/1‑151 (MQ=255)
                                                                                                                  cGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGaa                                 <  2:3795134/151‑1 (MQ=255)
                                                                                                                   gAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAAc                                <  2:479960/151‑1 (MQ=255)
                                                                                                                   gAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAAc                                <  1:3938427/151‑1 (MQ=255)
                                                                                                                       tGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTggg                            <  2:7309281/151‑1 (MQ=255)
                                                                                                                         ccGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTc                          >  1:1520942/1‑151 (MQ=255)
                                                                                                                             cggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccg                      <  1:10599026/151‑1 (MQ=255)
                                                                                                                              ggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccgg                     >  2:2143132/1‑151 (MQ=255)
                                                                                                                               gcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggc                    <  2:4115865/151‑1 (MQ=255)
                                                                                                                                   tGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGtt                <  1:9240506/151‑1 (MQ=255)
                                                                                                                                     ggCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGc              <  2:8342979/151‑1 (MQ=255)
                                                                                                                                      gCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCa             <  2:3012706/151‑1 (MQ=255)
                                                                                                                                        gACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGa           >  1:7355743/1‑151 (MQ=255)
                                                                                                                                         aCTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGaa          <  2:6703415/151‑1 (MQ=255)
                                                                                                                                           tGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGc        <  2:5963904/151‑1 (MQ=255)
                                                                                                                                            gTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCt       >  1:7017985/1‑151 (MQ=255)
                                                                                                                                             tCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTg      <  2:1873510/151‑1 (MQ=255)
                                                                                                                                               cggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt    >  2:140448/1‑151 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   >  2:2213970/1‑151 (MQ=255)
                                                                                                                                                 gcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGc  >  2:194949/1‑151 (MQ=255)
                                                                                                                                                 gcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGc  >  2:3650515/1‑151 (MQ=255)
                                                                                                                                                 gcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGc  <  2:7250363/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑