Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,468 A→G L277L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,4680AG100.0% 1688.8 / NA 419L277L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (223/196);  total (223/196)

Only 100 of 419 total aligned reads displayed.

TCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCAG  >  NC_008463/2955320‑2955615
                                                                                                                                                    |                                                                                                                                                   
     gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                                                                                                                                              <  1:724010/151‑1 (MQ=255)
     gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                                                                                                                                              >  1:418958/1‑151 (MQ=255)
       cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgc                                                                                                                                            <  1:9119117/151‑1 (MQ=255)
        gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                                                                                                                                           >  1:4854993/1‑151 (MQ=255)
          aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                                                                                                                                         <  2:10581519/151‑1 (MQ=255)
           cTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTg                                                                                                                                        >  1:6237296/1‑151 (MQ=255)
            tGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                                                                                                                                       >  2:2491226/1‑151 (MQ=255)
             gCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCGGGc                                                                                                                                      <  2:8966559/151‑1 (MQ=255)
              cAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGcc                                                                                                                                     >  1:8874709/1‑151 (MQ=255)
               aGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                                                                                                                                    >  1:1605661/1‑151 (MQ=255)
                      tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt                                                                                                                             <  1:4019267/151‑1 (MQ=255)
                         ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                                                          >  2:8243056/1‑151 (MQ=255)
                         ggTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg                                                                                                                          >  2:4431298/1‑151 (MQ=255)
                            cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                                                       >  1:7621186/1‑151 (MQ=255)
                             gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                      >  1:7420797/1‑151 (MQ=255)
                             gCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa                                                                                                                      >  1:4422179/1‑151 (MQ=255)
                              cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc                                                                                                                     >  1:8787844/1‑151 (MQ=255)
                                    aggCGGGTGGTTTCGATCTTGCCGGTATACGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCgg                                                                                                               <  2:9418992/149‑1 (MQ=255)
                                        gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                                                           <  2:7751834/151‑1 (MQ=255)
                                        gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                                                           >  2:3171859/1‑151 (MQ=255)
                                        gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                                                           >  1:5213645/1‑151 (MQ=255)
                                        gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                                                           <  1:5670634/151‑1 (MQ=255)
                                              tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                                                     >  1:8601638/1‑151 (MQ=255)
                                               ttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGc                                                                                                    >  1:9540199/1‑151 (MQ=255)
                                                 cGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAc                                                                                                  <  1:10085707/151‑1 (MQ=255)
                                                  gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                 <  1:3673099/151‑1 (MQ=255)
                                                  gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                                                                 >  2:800519/1‑151 (MQ=255)
                                                    tCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGc                                                                                               >  2:4539756/1‑151 (MQ=255)
                                                     cTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGcc                                                                                              >  1:8669204/1‑151 (MQ=255)
                                                      ttGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGATGGGGATACGCCCGGTGACGAGCCGCACCGCCt                                                                                             <  1:8971058/151‑1 (MQ=255)
                                                       tGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTc                                                                                            <  2:3408126/151‑1 (MQ=255)
                                                       tGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTc                                                                                            <  1:5816862/151‑1 (MQ=255)
                                                          cGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTc                                                                                         >  1:2044638/1‑151 (MQ=255)
                                                            gTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCa                                                                                       <  2:193284/151‑1 (MQ=255)
                                                             tataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAg                                                                                      >  2:7332181/1‑151 (MQ=255)
                                                              ataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGACCAGc                                                                                     >  1:10317664/1‑151 (MQ=255)
                                                               taAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCt                                                                                    <  2:232098/151‑1 (MQ=255)
                                                                 aGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCg                                                                                  <  2:10440522/151‑1 (MQ=255)
                                                                 aGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCg                                                                                  <  2:5336218/151‑1 (MQ=255)
                                                                 aGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCg                                                                                  >  1:4240774/1‑151 (MQ=255)
                                                                   ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                                >  2:6841790/1‑151 (MQ=255)
                                                                   ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                                <  1:9954761/151‑1 (MQ=255)
                                                                   ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                                <  2:6963240/151‑1 (MQ=255)
                                                                   ggTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAt                                                                                <  1:10197720/151‑1 (MQ=255)
                                                                      tCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTa                                                                             >  2:4683652/1‑151 (MQ=255)
                                                                        aGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATg                                                                           <  1:10689563/151‑1 (MQ=255)
                                                                         gcgcGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATgg                                                                          <  1:2673313/151‑1 (MQ=255)
                                                                           gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                                                        >  2:7536430/1‑151 (MQ=255)
                                                                                cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                                                   >  2:1116890/1‑151 (MQ=255)
                                                                                cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                                                   <  2:2044599/151‑1 (MQ=255)
                                                                                cGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTc                                                                   <  2:1325142/151‑1 (MQ=255)
                                                                                 gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                                                                  >  2:1461844/1‑151 (MQ=255)
                                                                                 gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                                                                  >  1:7559159/1‑151 (MQ=255)
                                                                                   cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                                                                >  1:5616240/1‑151 (MQ=255)
                                                                                   cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                                                                <  2:2622943/151‑1 (MQ=255)
                                                                                   cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                                                                >  1:1884757/1‑151 (MQ=255)
                                                                                   cTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGa                                                                <  2:4221405/151‑1 (MQ=255)
                                                                                       aaCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCgg                                                            <  1:4625564/151‑1 (MQ=255)
                                                                                        aCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCggg                                                           >  2:8663879/1‑151 (MQ=255)
                                                                                         cATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGt                                                          <  2:294514/151‑1 (MQ=255)
                                                                                             aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                      <  1:5381028/151‑1 (MQ=255)
                                                                                             aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                                                      >  1:6891908/1‑151 (MQ=255)
                                                                                               cGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCCGGCTGGGGATACGCCCGGTGACGAGCCGCCCCGCCTCGTCCAGCTCGATGTATGGAACCTTCGGACCGGGTgcggcg                                                    <  2:10155826/151‑1 (MQ=255)
                                                                                               cGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcggcg                                                    >  2:1612206/1‑151 (MQ=255)
                                                                                                ggATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGgcgc                                                   >  1:6559827/1‑151 (MQ=255)
                                                                                                ggATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGgcgc                                                   >  1:3065486/1‑151 (MQ=255)
                                                                                                     ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc                                              >  2:7443630/1‑151 (MQ=255)
                                                                                                     ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc                                              >  2:2781880/1‑151 (MQ=255)
                                                                                                     ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc                                              >  1:9162814/1‑151 (MQ=255)
                                                                                                       aGCCGCGTCCGGCCACGAACATCTGGCTGTTGGCGTTGCCGGGGAGGAACACCGCGCGGGCCTCGCGGCTGGGGCTACGCCCGGTGACGAGCCGCACCGCCACGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCAc                                            <  1:644007/151‑1 (MQ=255)
                                                                                                       aGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGACCACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCAc                                            <  1:9381163/151‑1 (MQ=255)
                                                                                                        gCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg                                           <  2:3802506/151‑1 (MQ=255)
                                                                                                        gCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGGTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg                                           <  2:10055979/151‑1 (MQ=255)
                                                                                                            cgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg                                       <  1:9651681/151‑1 (MQ=255)
                                                                                                             gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc                                      <  1:928073/151‑1 (MQ=255)
                                                                                                              tCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgcg                                     >  1:8271371/1‑151 (MQ=255)
                                                                                                                 ggCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGt                                  >  1:3570564/2‑151 (MQ=255)
                                                                                                                    cACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCg                               >  2:9638800/1‑151 (MQ=255)
                                                                                                                    cACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCg                               >  1:3794961/1‑151 (MQ=255)
                                                                                                                    cACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCg                               >  1:8112932/1‑151 (MQ=255)
                                                                                                                     aCGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGa                              >  2:10485986/1‑151 (MQ=255)
                                                                                                                      cGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGAt                             >  2:4896966/1‑151 (MQ=255)
                                                                                                                        aaCATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGa                           <  2:5826635/151‑1 (MQ=255)
                                                                                                                        aaCATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGa                           <  1:3402827/151‑1 (MQ=255)
                                                                                                                         aCATCGGGCTGTAGGCGGTTCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGCGCCTCACCGCCTCGTCCAGCTCGATGTAAGGAACCTTCTGACCGGGTGCGGCGCGGGGCCCGTCGGCGCGAGCGATGAc                          <  2:3912916/151‑1 (MQ=255)
                                                                                                                           aTCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCg                        >  2:643468/1‑151 (MQ=255)
                                                                                                                              gggCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAg                     >  1:3226581/1‑151 (MQ=255)
                                                                                                                                  tGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGAt                 <  2:8846508/151‑1 (MQ=255)
                                                                                                                                  tGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGAt                 <  1:1336377/151‑1 (MQ=255)
                                                                                                                                    tAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGc               <  1:4991886/151‑1 (MQ=255)
                                                                                                                                     aGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGcc              >  2:5631324/1‑151 (MQ=255)
                                                                                                                                      ggCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGTTGTCCGGAGGGATGCcg             >  2:5505245/1‑151 (MQ=255)
                                                                                                                                        cGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtc           <  2:8714786/151‑1 (MQ=255)
                                                                                                                                         gTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCCCCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGAGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCcgtcg          <  1:2215767/151‑1 (MQ=255)
                                                                                                                                           tGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGtt        >  2:8976570/1‑151 (MQ=255)
                                                                                                                                           tGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGtt        <  2:6897561/151‑1 (MQ=255)
                                                                                                                                              cGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGt     >  2:6300216/1‑151 (MQ=255)
                                                                                                                                               gggCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTc    >  2:609535/1‑151 (MQ=255)
                                                                                                                                                ggCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCa   >  2:8442061/1‑151 (MQ=255)
                                                                                                                                                 gCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCAg  <  2:3689769/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCGCGTGCGATGACCGGAGGGATGCCGTCGTTGGTCAG  >  NC_008463/2955320‑2955615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑