Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,443,2920CG100.0% 1811.3 / NA 449P459A (CCC→GCC) PA14_RS15680GntP family permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (216/233);  total (216/233)

Only 100 of 453 total aligned reads displayed.

CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCT  >  NC_008463/3443144‑3443437
                                                                                                                                                    |                                                                                                                                                 
cggcggGATGGATACCCTGCCGCACACCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTAcgcc                                                                                                                                                 <  2:2450632/151‑1 (MQ=255)
 ggcggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCa                                                                                                                                                >  1:7729933/1‑151 (MQ=255)
   cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                              >  2:3737794/1‑151 (MQ=255)
   cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                              <  2:5670416/151‑1 (MQ=255)
   cggGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                              >  2:1031869/1‑151 (MQ=255)
        tggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCACGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCt                                                                                                                                         <  2:1493115/151‑1 (MQ=255)
         ggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                        <  2:6798341/151‑1 (MQ=255)
         ggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                        >  1:3792751/1‑151 (MQ=255)
           atACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGt                                                                                                                                      <  1:7895957/151‑1 (MQ=255)
                  gCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATcc                                                                                                                               <  1:7317458/151‑1 (MQ=255)
                  gCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATcc                                                                                                                               >  1:2802510/1‑151 (MQ=255)
                   ccGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCg                                                                                                                              >  2:3929028/1‑151 (MQ=255)
                    cGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGc                                                                                                                             <  1:9599901/151‑1 (MQ=255)
                      cacaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGccc                                                                                                                           <  2:1908803/151‑1 (MQ=255)
                      cacaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGccc                                                                                                                           <  2:709271/151‑1 (MQ=255)
                        caACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtt                                                                                                                         >  2:2757813/1‑151 (MQ=255)
                         aaCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttt                                                                                                                        >  1:5300208/1‑151 (MQ=255)
                            ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                     >  1:2238327/1‑151 (MQ=255)
                            ggCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttttt                                                                                                                     <  1:7588749/151‑1 (MQ=255)
                              cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGTTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                   <  2:4587898/151‑1 (MQ=255)
                              cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                   <  1:3615742/151‑1 (MQ=255)
                              cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                   <  1:8122439/151‑1 (MQ=255)
                                 ggTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGGTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATcc                                                                                                                >  1:10514441/1‑151 (MQ=255)
                                       cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                          <  1:2543268/151‑1 (MQ=255)
                                       cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                          <  1:1867378/151‑1 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                         <  1:6185413/151‑1 (MQ=255)
                                             cctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAAc                                                                                                    <  1:3480853/151‑1 (MQ=255)
                                              ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                   <  2:3571026/151‑1 (MQ=255)
                                              ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                   <  2:2937580/151‑1 (MQ=255)
                                              ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                   >  2:7199917/1‑151 (MQ=255)
                                                ggCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGc                                                                                                 <  1:7053601/151‑1 (MQ=255)
                                                 gCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCa                                                                                                >  1:5068385/1‑151 (MQ=255)
                                                   ggTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATg                                                                                              >  2:1333165/1‑151 (MQ=255)
                                                    gTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGa                                                                                             >  1:1329498/1‑151 (MQ=255)
                                                    gTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGa                                                                                             <  2:2830728/151‑1 (MQ=255)
                                                       aCCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAccc                                                                                          <  1:8333701/151‑1 (MQ=255)
                                                        ccGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCt                                                                                         >  2:3429825/1‑151 (MQ=255)
                                                         cGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                        >  2:4338682/1‑151 (MQ=255)
                                                          ggCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGa                                                                                       <  1:8515513/151‑1 (MQ=255)
                                                             cTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAg                                                                                    <  2:6328830/151‑1 (MQ=255)
                                                                 cccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCaa                                                                                <  2:6554375/151‑1 (MQ=255)
                                                                  ccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAg                                                                               <  1:8096010/151‑1 (MQ=255)
                                                                  ccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAg                                                                               >  1:4615418/1‑151 (MQ=255)
                                                                  ccATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAg                                                                               <  1:3907067/151‑1 (MQ=255)
                                                                     tCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGAcc                                                                            >  2:2590729/1‑151 (MQ=255)
                                                                       gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                          >  2:2212312/1‑151 (MQ=255)
                                                                        ggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGcc                                                                         <  2:5110999/151‑1 (MQ=255)
                                                                           ggCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTg                                                                      <  2:961712/151‑1 (MQ=255)
                                                                             cGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGt                                                                    >  2:4698705/1‑151 (MQ=255)
                                                                              gTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTc                                                                   <  2:8818174/151‑1 (MQ=255)
                                                                               tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCa                                                                  >  1:1721790/1‑151 (MQ=255)
                                                                                 cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                >  2:8456182/1‑151 (MQ=255)
                                                                                 cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGACCTGGTCAcc                                                                >  2:616701/1‑151 (MQ=255)
                                                                                     gACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGtt                                                            >  1:2900036/1‑151 (MQ=255)
                                                                                      aCATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTc                                                           >  2:9672671/1‑151 (MQ=255)
                                                                                         ttttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccac                                                        >  2:4156928/1‑151 (MQ=255)
                                                                                           ttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccacca                                                      <  2:2372469/151‑1 (MQ=255)
                                                                                           ttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccacca                                                      >  1:500124/1‑151 (MQ=255)
                                                                                             cgtcATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCTGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCATTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGc                                                    <  2:5180287/148‑1 (MQ=255)
                                                                                             cGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGc                                                    <  1:2695307/151‑1 (MQ=255)
                                                                                                cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                 >  1:5985992/1‑151 (MQ=255)
                                                                                                 aTTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCa                                                >  1:9990740/1‑151 (MQ=255)
                                                                                                   tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                              <  1:8652326/151‑1 (MQ=255)
                                                                                                     cccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCAACCGGCCTGGTCTGATCCGCCCTATTTTAATCCTGCGGGAGAAACAGCATGACCCTGAAATGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCgg                                            >  1:4236293/1‑151 (MQ=255)
                                                                                                      ccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                           >  1:9149588/1‑151 (MQ=255)
                                                                                                      ccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                           >  2:8721620/1‑151 (MQ=255)
                                                                                                       cTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGcc                                          >  1:10018764/1‑151 (MQ=255)
                                                                                                        tGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                         >  2:7518949/1‑151 (MQ=255)
                                                                                                          aTCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                       <  1:1227332/151‑1 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTGTTCGTCTTCGGTCTGTTCTACGCCACCGGCCTGTTCTGATCCTCGCTTTGTTCATCATGCGGGAGTAACAGCATGACCCAGTAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                  <  2:9159619/151‑1 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                  >  1:9344257/1‑151 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                  <  2:9011560/151‑1 (MQ=255)
                                                                                                                aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCg                                 >  2:1774813/1‑151 (MQ=255)
                                                                                                                  ccTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGcc                               >  1:6783084/1‑151 (MQ=255)
                                                                                                                  ccTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGcc                               >  1:3154761/1‑151 (MQ=255)
                                                                                                                       cGGTGTTCTTCGTCCTCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAg                          <  1:10408219/151‑1 (MQ=255)
                                                                                                                       cGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAg                          >  2:5623887/1‑151 (MQ=255)
                                                                                                                       cGGTGTTCTACGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTATTACATCCTGCGGGAGAAACAGCATGACCCTGACAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAg                          <  2:592575/151‑1 (MQ=255)
                                                                                                                         gtgtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGcc                        <  1:1635606/151‑1 (MQ=255)
                                                                                                                           gtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTg                      <  1:5577380/151‑1 (MQ=255)
                                                                                                                             tcttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGc                    >  1:1074104/1‑151 (MQ=255)
                                                                                                                              cttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGcc                   <  1:6050262/151‑1 (MQ=255)
                                                                                                                                tcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGa                 >  2:10722088/1‑151 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                <  1:648044/151‑1 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                <  1:8823336/151‑1 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                <  1:9467658/151‑1 (MQ=255)
                                                                                                                                  gTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAgg               >  1:2061266/1‑151 (MQ=255)
                                                                                                                                   tCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGc              >  2:4550605/1‑151 (MQ=255)
                                                                                                                                    cATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGGGCCACCAGCGGCCTCGGCCTGGGCGTCGCCCTGAGCCTGGCCGAGGcc             >  2:7343627/1‑151 (MQ=255)
                                                                                                                                    cATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTACATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGcc             <  2:7795558/151‑1 (MQ=255)
                                                                                                                                      tCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCgg           >  1:10122310/1‑151 (MQ=255)
                                                                                                                                       cGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgc          >  2:8214313/1‑151 (MQ=255)
                                                                                                                                        ggACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgcg         >  2:3157775/1‑151 (MQ=255)
                                                                                                                                        ggACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgcg         >  2:2061509/1‑151 (MQ=255)
                                                                                                                                           cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg      >  2:6708862/1‑151 (MQ=255)
                                                                                                                                           cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg      <  2:4818686/151‑1 (MQ=255)
                                                                                                                                           cTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCg      <  1:5938320/151‑1 (MQ=255)
                                                                                                                                               tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCt  <  2:7911925/151‑1 (MQ=255)
                                                                                                                                               tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCt  >  2:6042155/1‑151 (MQ=255)
                                                                                                                                               tCTACGCCACCGGCCTGGTCTGATCCGCCCTTATTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCt  <  2:9763576/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCT  >  NC_008463/3443144‑3443437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑