Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,407,236 A→G intergenic (+107/‑205) PA14_RS20115 → / → toxA ATP‑grasp domain‑containing protein/exotoxin A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,407,2360AG99.4% 1402.4 / NA 357intergenic (+107/‑205)PA14_RS20115/toxAATP‑grasp domain‑containing protein/exotoxin A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (155/200);  minor base T (1/1);  total (156/201)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GGCCCGCCGCACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGAAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCTCGCCCGAC  >  NC_008463/4407089‑4407383
                                                                                                                                                   |                                                                                                                                                   
ggCCCGCCGCTCGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCATCCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAAc                                                                                                                                                  <  1:8638348/151‑1 (MQ=255)
 gCCCGCCGCACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACg                                                                                                                                                 <  2:9523527/151‑1 (MQ=255)
  cccGCCGCACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACgg                                                                                                                                                <  1:4374023/151‑1 (MQ=255)
  cccGCCGCACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACgg                                                                                                                                                <  1:9544925/151‑1 (MQ=255)
    cgccgcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGtt                                                                                                                                              <  1:3718201/151‑1 (MQ=255)
     gccgcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTg                                                                                                                                             >  1:8697117/1‑151 (MQ=255)
      ccgcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCACTCCCAACCCGACCAAGCGCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgc                                                                                                                                            <  1:2591386/151‑2 (MQ=255)
       cgcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgtc                                                                                                                                           <  1:10437922/151‑1 (MQ=255)
       cgcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgtc                                                                                                                                           >  2:4080775/1‑151 (MQ=255)
        gcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgtcc                                                                                                                                          >  2:5036933/1‑151 (MQ=255)
        gcACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgtcc                                                                                                                                          <  1:3269231/151‑1 (MQ=255)
          aCGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTgtccgt                                                                                                                                        <  1:8726085/151‑1 (MQ=255)
              ttCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTcccc                                                                                                                                    >  2:6449428/1‑151 (MQ=255)
                cgcgGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTc                                                                                                                                  <  2:10485487/151‑1 (MQ=255)
                 gcggcgATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCACCCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCg                                                                                                                                 <  2:4431138/151‑1 (MQ=255)
                 gcggcgATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCg                                                                                                                                 <  2:9640335/151‑1 (MQ=255)
                      gATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCAt                                                                                                                            <  1:6897472/151‑1 (MQ=255)
                      gATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCAt                                                                                                                            <  2:5912448/151‑1 (MQ=255)
                       aTGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATg                                                                                                                           >  2:6477182/1‑151 (MQ=255)
                        tGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATgg                                                                                                                          <  2:5369907/151‑1 (MQ=255)
                          cTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGat                                                                                                                        <  2:3118644/151‑1 (MQ=255)
                          cTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGat                                                                                                                        <  2:4340101/151‑1 (MQ=255)
                          cTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGat                                                                                                                        >  2:7932218/1‑151 (MQ=255)
                             gCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATAtt                                                                                                                     >  2:4988916/1‑151 (MQ=255)
                              cacaGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATAttt                                                                                                                    <  1:4957068/151‑1 (MQ=255)
                               acaGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATttc                                                                                                                   <  2:3967390/151‑1 (MQ=255)
                                caGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATttct                                                                                                                  >  1:10574710/1‑151 (MQ=255)
                                 aGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATttctt                                                                                                                 >  2:2148002/1‑151 (MQ=255)
                                 aGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGACCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATttctt                                                                                                                 >  2:2374425/1‑151 (MQ=255)
                                  ggCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATttcttc                                                                                                                <  2:1795355/151‑1 (MQ=255)
                                    cATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCa                                                                                                              <  2:118709/151‑1 (MQ=255)
                                     aTAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCAc                                                                                                             >  1:4102188/1‑151 (MQ=255)
                                      tAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCAct                                                                                                            >  2:10712857/1‑151 (MQ=255)
                                        gTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCActct                                                                                                          <  1:101676/151‑1 (MQ=255)
                                          cGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGc                                                                                                        >  1:2744371/1‑151 (MQ=255)
                                          cGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGc                                                                                                        <  2:1005949/151‑1 (MQ=255)
                                           ggAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCa                                                                                                       <  1:6668412/151‑1 (MQ=255)
                                            gAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGACCCCTCGCCCATGGATATTTCTTCCACTCTGCaa                                                                                                      >  2:2725893/1‑151 (MQ=255)
                                             aaCCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAAt                                                                                                     <  1:7144567/151‑1 (MQ=255)
                                             aaCCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAAt                                                                                                     <  1:5321271/151‑1 (MQ=255)
                                               ccGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATcc                                                                                                   <  1:4061907/151‑1 (MQ=255)
                                                cGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCa                                                                                                  >  1:672294/1‑151 (MQ=255)
                                                  cTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGt                                                                                                <  1:7926788/151‑1 (MQ=255)
                                                  cTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGt                                                                                                >  1:3178761/1‑151 (MQ=255)
                                                   tGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGtt                                                                                               >  1:2247185/1‑151 (MQ=255)
                                                   tGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGtt                                                                                               <  1:10313872/151‑1 (MQ=255)
                                                   tGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGtt                                                                                               >  1:3799417/1‑151 (MQ=255)
                                                   tGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGtt                                                                                               <  1:4810105/151‑1 (MQ=255)
                                                   tGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGtt                                                                                               >  1:9612629/1‑151 (MQ=255)
                                                    gCACTCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTc                                                                                              <  2:6990602/151‑1 (MQ=255)
                                                         cccAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATaaa                                                                                         <  1:1958023/151‑1 (MQ=255)
                                                         cccAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATaaa                                                                                         <  2:4640429/151‑1 (MQ=255)
                                                          ccAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAAt                                                                                        <  2:319382/151‑1 (MQ=255)
                                                           cAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATc                                                                                       <  2:4171592/151‑1 (MQ=255)
                                                           cAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATc                                                                                       >  2:4485580/1‑151 (MQ=255)
                                                            aaCCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATcc                                                                                      <  1:10119020/151‑1 (MQ=255)
                                                                cGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATa                                                                                  <  1:8973342/151‑1 (MQ=255)
                                                                 gACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATaa                                                                                 >  1:5543820/1‑151 (MQ=255)
                                                                 gACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATaa                                                                                 <  1:8206722/151‑1 (MQ=255)
                                                                   ccAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATaaaa                                                                               <  1:1524423/151‑1 (MQ=255)
                                                                     aaGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGc                                                                             >  2:9221155/1‑151 (MQ=255)
                                                                       gCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGccc                                                                           <  1:7483120/151‑1 (MQ=255)
                                                                        cTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCAAGGAACGGTTGTCCGTCCCCTCGCCCAGGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCct                                                                          <  2:8164355/151‑1 (MQ=255)
                                                                          ccccTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCctct                                                                        <  2:1865798/151‑1 (MQ=255)
                                                                          ccccTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCctct                                                                        <  1:5647699/151‑1 (MQ=255)
                                                                           cccTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCtt                                                                       <  2:1494378/151‑1 (MQ=255)
                                                                            ccTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTc                                                                      <  2:3687623/151‑1 (MQ=255)
                                                                              ttGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCg                                                                    >  1:295226/1‑151 (MQ=255)
                                                                               tGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGc                                                                   >  2:67082/1‑151 (MQ=255)
                                                                                gatcgatcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCt                                                                  <  1:8037721/151‑1 (MQ=255)
                                                                                  tcgatcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTcc                                                                <  1:3371455/151‑1 (MQ=255)
                                                                                  tcgatcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTcc                                                                <  1:6424425/151‑1 (MQ=255)
                                                                                   cgatcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCAGAAATCCCATAAAAGCCCTCTTCCGCTccc                                                               >  1:6764866/1‑151 (MQ=255)
                                                                                      tcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGc                                                            >  1:8261432/1‑151 (MQ=255)
                                                                                      tcCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGc                                                            >  2:6157150/1‑151 (MQ=255)
                                                                                       cccGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGcc                                                           <  1:246671/151‑1 (MQ=255)
                                                                                        ccGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATGTCTTCCACTCTTCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCa                                                          <  1:159119/151‑1 (MQ=255)
                                                                                           aGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGcc                                                       <  2:771204/151‑1 (MQ=255)
                                                                                           aGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGCTTTACCGTCCACTAGCCCAAGGATATTTCAAACACTCAGCAATCAAGTTCATAAATCCAATAAAATCCCTCTTCAGCTCCCCGCCAgac                                                       >  2:5694482/1‑149 (MQ=255)
                                                                                            ggggggCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCt                                                      >  1:9196573/1‑151 (MQ=255)
                                                                                                ggCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTcccc                                                  <  1:1292238/151‑1 (MQ=255)
                                                                                                ggCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTcccc                                                  <  2:5765768/151‑1 (MQ=255)
                                                                                                            gCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCAcc                                      >  2:8386536/1‑151 (MQ=255)
                                                                                                            gCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCAcc                                      <  1:9343299/151‑1 (MQ=255)
                                                                                                                     cTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGcc                             <  2:7398777/151‑1 (MQ=255)
                                                                                                                     cTCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGcc                             <  1:10679621/151‑1 (MQ=255)
                                                                                                                      tCGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATACATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGccc                            <  1:1133384/151‑1 (MQ=255)
                                                                                                                       cGACCACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGcccc                           <  2:8261432/151‑1 (MQ=255)
                                                                                                                           cACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCccgccg                       >  1:9781806/1‑151 (MQ=255)
                                                                                                                           cACTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCccgccg                       >  1:8576504/1‑151 (MQ=255)
                                                                                                                            aCTTACTCTCATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATGTCTTCCACACTGCAATCCAGTTCATAATTACCATAAAAGCCGTCTTGGGCTCGCCGGCTGCCGCCCCGCATCGCGCACCCTGGACGGCCcgcggc                      >  2:377476/1‑151 (MQ=255)
                                                                                                                                 ctctCATCAGAAGCACATGTACGGTTTTCCGTCCCCTCGCCCTTGGTTATTTCTTCCACTCTGCTATACAGTTCATTAATCCCATTAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGAATCCCGAACCCTAGACGCCCCGCCGCTCTcc                 <  2:9681714/151‑1 (MQ=255)
                                                                                                                                  tctcATCCGCAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATTAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTccg                <  2:8789888/151‑1 (MQ=255)
                                                                                                                                    tcATCCGAAGCCCAGGAACGGGTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTccgcc              <  1:1373204/151‑1 (MQ=255)
                                                                                                                                     cATCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTccgccg             <  2:2561110/151‑1 (MQ=255)
                                                                                                                                      aTCCGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCgg            <  2:9028612/151‑1 (MQ=255)
                                                                                                                                        ccGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGGTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCt          >  1:8357054/1‑151 (MQ=255)
                                                                                                                                         cGAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCTc         <  2:7505185/151‑1 (MQ=255)
                                                                                                                                          gAAGCCCAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCTCg        <  1:5207690/151‑1 (MQ=255)
                                                                                                                                                cAGGAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCTCGCCCGAc  >  1:606865/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCCCGCCGCACGCTTCGCGGCGATGCTGGCACAGGCATAGTCGGAACCGCTGCAATCCCAACCCGACCAAGCTCCCCTTGATCGATCCCGAGGGGGGCTCGTCACTCGCCCAGTGCCTCGACCACTTACTCTCATCCGAAGCCCAGAAACGGTTGTCCGTCCCCTCGCCCATGGATATTTCTTCCACTCTGCAATCCAGTTCATAAATCCCATAAAAGCCCTCTTCCGCTCCCCGCCAGCCTCCCCGCATCCCGCACCCTAGACGCCCCGCCGCTCTCCGCCGGCTCGCCCGAC  >  NC_008463/4407089‑4407383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑