Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,005 C→G G319A (GGG→GCG)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0050CG99.8% 2237.8 / NA 555G319A (GGG→GCG) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (257/297);  total (257/298)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 557 total aligned reads displayed.

CAGCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCG  >  NC_008463/4564857‑4565152
                                                                                                                                                    |                                                                                                                                                   
cAGCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCcgcg                                                                                                                                                   >  1:7515114/1‑151 (MQ=255)
cAGCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCcgcg                                                                                                                                                   <  1:9594775/151‑1 (MQ=255)
 aGCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGcgt                                                                                                                                                  <  2:8898052/151‑1 (MQ=255)
  gCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGcgtc                                                                                                                                                 <  2:4962596/151‑1 (MQ=255)
      catGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTAc                                                                                                                                             >  2:10622748/1‑151 (MQ=255)
      catGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTAc                                                                                                                                             <  1:2192694/151‑1 (MQ=255)
         gtgCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTc                                                                                                                                          <  2:1559703/151‑1 (MQ=255)
            cTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAAt                                                                                                                                       >  1:5819615/1‑151 (MQ=255)
            cTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAAt                                                                                                                                       >  1:6227107/1‑151 (MQ=255)
            cTCGGCGATCTCCTGGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAAt                                                                                                                                       <  1:998234/151‑1 (MQ=255)
              cGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGc                                                                                                                                     >  1:6601448/1‑151 (MQ=255)
              cGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGAATGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGc                                                                                                                                     >  2:3147796/1‑151 (MQ=255)
               ggCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGcc                                                                                                                                    <  1:2291545/151‑1 (MQ=255)
               ggCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGcc                                                                                                                                    <  2:940735/151‑1 (MQ=255)
                gCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCg                                                                                                                                   <  2:4122535/151‑1 (MQ=255)
                 cGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGa                                                                                                                                  <  1:7363595/151‑1 (MQ=255)
                 cGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGa                                                                                                                                  >  2:10542807/1‑151 (MQ=255)
                   aTCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACt                                                                                                                                <  2:8752385/151‑1 (MQ=255)
                   aTCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACt                                                                                                                                >  2:4779223/1‑151 (MQ=255)
                    tctcCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTc                                                                                                                               >  1:2576001/1‑151 (MQ=255)
                       ccTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCgg                                                                                                                            <  2:10256060/151‑1 (MQ=255)
                         ttGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCg                                                                                                                          <  1:6428359/151‑1 (MQ=255)
                          tGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGa                                                                                                                         >  2:6688712/1‑151 (MQ=255)
                            gCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAg                                                                                                                       <  1:6076336/151‑1 (MQ=255)
                              gTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAgcg                                                                                                                     <  1:4984715/151‑1 (MQ=255)
                               tCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAgcgc                                                                                                                    >  2:2771220/1‑151 (MQ=255)
                                ccAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGcc                                                                                                                   >  2:22780/1‑151 (MQ=255)
                                ccAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGcc                                                                                                                   >  1:1268873/1‑151 (MQ=255)
                                ccAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGcc                                                                                                                   >  2:3352284/1‑151 (MQ=255)
                                 cAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGccg                                                                                                                  >  2:9281258/1‑150 (MQ=255)
                                   ggCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCgaa                                                                                                                >  2:5640159/1‑151 (MQ=255)
                                     cgcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACa                                                                                                              <  1:651236/151‑1 (MQ=255)
                                              gCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGt                                                                                                     <  1:2711023/151‑1 (MQ=255)
                                              gCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGt                                                                                                     <  1:10259178/151‑1 (MQ=255)
                                                ttCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcc                                                                                                   <  2:4519189/151‑1 (MQ=255)
                                                   cAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTccccc                                                                                                <  2:5713586/151‑1 (MQ=255)
                                                   cAGGCGATTGTCCAGGTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTccccc                                                                                                <  1:8728743/151‑1 (MQ=255)
                                                     ggCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGc                                                                                              >  1:4584088/1‑151 (MQ=255)
                                                      gCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                             >  1:3300495/1‑151 (MQ=255)
                                                      gCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                             >  2:1749991/1‑151 (MQ=255)
                                                        gATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATc                                                                                           <  1:4256538/151‑1 (MQ=255)
                                                         aTTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCg                                                                                          >  1:7970750/1‑151 (MQ=255)
                                                           tGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                        >  2:4540998/1‑151 (MQ=255)
                                                              ccAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaa                                                                                     <  1:3485871/151‑1 (MQ=255)
                                                               cAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaag                                                                                    <  1:8845740/151‑1 (MQ=255)
                                                                aGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGTGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaaga                                                                                   <  1:10684429/151‑1 (MQ=255)
                                                                aGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaaga                                                                                   >  1:6812861/1‑151 (MQ=255)
                                                                 gTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaagaa                                                                                  <  1:840482/151‑1 (MQ=255)
                                                                   tCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGc                                                                                <  2:9692313/151‑1 (MQ=255)
                                                                   tCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGc                                                                                <  2:3733418/151‑1 (MQ=255)
                                                                   tCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGAGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGc                                                                                <  1:2992529/151‑1 (MQ=255)
                                                                     cGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACTGGCAGAAGTCCCCCGCATCGAGGAAGAAGCgg                                                                              >  1:5554040/1‑151 (MQ=255)
                                                                      gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                             <  1:9761812/151‑1 (MQ=255)
                                                                      gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                             >  1:5957311/1‑151 (MQ=255)
                                                                      gCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGt                                                                             <  1:5372261/151‑1 (MQ=255)
                                                                         tCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACg                                                                          <  1:1324402/151‑1 (MQ=255)
                                                                          cGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACgg                                                                         <  1:3642427/151‑1 (MQ=255)
                                                                           gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                        >  1:7133299/1‑151 (MQ=255)
                                                                           gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                        >  2:6229338/1‑151 (MQ=255)
                                                                               gcgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGACGCGGTACGGGCTgg                                                                    >  2:8222385/1‑151 (MQ=255)
                                                                               gcgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgg                                                                    <  1:9018352/151‑1 (MQ=255)
                                                                                cgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGACGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTggg                                                                   >  2:2923940/1‑151 (MQ=255)
                                                                                  ccccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGt                                                                 >  2:5170352/1‑151 (MQ=255)
                                                                                     cGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGc                                                              <  1:3519756/151‑1 (MQ=255)
                                                                                          aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                         <  2:7964233/151‑1 (MQ=255)
                                                                                           tCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGc                                                        <  2:9830476/151‑1 (MQ=255)
                                                                                               gTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACaa                                                    >  2:903502/1‑151 (MQ=255)
                                                                                                  cgcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTg                                                 <  2:7342994/151‑1 (MQ=255)
                                                                                                  cgcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTg                                                 <  2:7111986/151‑1 (MQ=255)
                                                                                                   gcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTgg                                                >  2:2108485/1‑151 (MQ=255)
                                                                                                         gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAg                                          <  2:3995724/151‑1 (MQ=255)
                                                                                                          cGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                         <  1:5766106/151‑1 (MQ=255)
                                                                                                              tGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCg                                     <  2:2590992/151‑1 (MQ=255)
                                                                                                              tGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCg                                     >  1:7688965/1‑151 (MQ=255)
                                                                                                                ccGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGcc                                   <  2:10317227/151‑1 (MQ=255)
                                                                                                                    cAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                               >  1:1121571/1‑151 (MQ=255)
                                                                                                                    cAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                               <  1:5138446/151‑1 (MQ=255)
                                                                                                                     aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                              <  2:1315951/151‑1 (MQ=255)
                                                                                                                     aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                              >  1:5468889/1‑151 (MQ=255)
                                                                                                                      aTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGt                             <  1:2902979/151‑1 (MQ=255)
                                                                                                                       tGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTc                            >  2:2940602/1‑151 (MQ=255)
                                                                                                                        ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                           <  1:4692864/151‑1 (MQ=255)
                                                                                                                        ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                           <  1:10078380/151‑1 (MQ=255)
                                                                                                                        ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                           >  2:3107848/1‑151 (MQ=255)
                                                                                                                          ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                         <  2:7648697/151‑1 (MQ=255)
                                                                                                                          ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                         <  1:10395643/151‑1 (MQ=255)
                                                                                                                                gTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACgg                   >  2:6591372/1‑151 (MQ=255)
                                                                                                                                gTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACgg                   >  2:4399372/1‑151 (MQ=255)
                                                                                                                                 ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                  >  1:9200537/1‑151 (MQ=255)
                                                                                                                                 ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                  <  1:1924639/151‑1 (MQ=255)
                                                                                                                                 ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                  >  2:1009606/1‑151 (MQ=255)
                                                                                                                                  tCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCa                 >  2:8067414/1‑151 (MQ=255)
                                                                                                                                    cACCTCGCGACTCTCCGCGTCGTACTTCCATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg               >  1:8679166/1‑151 (MQ=255)
                                                                                                                                     aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc              >  2:4275017/1‑151 (MQ=255)
                                                                                                                                      ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt             <  1:3896422/151‑1 (MQ=255)
                                                                                                                                       cTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCNTGCTg            <  1:8181330/151‑1 (MQ=255)
                                                                                                                                         cgcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa          >  1:9336892/1‑151 (MQ=255)
                                                                                                                                          gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg         <  2:6274713/151‑1 (MQ=255)
                                                                                                                                           cgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGc        <  1:693763/151‑1 (MQ=255)
                                                                                                                                             aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg      >  1:1056195/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCG  >  NC_008463/4564857‑4565152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑