Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 480,915 T→C K193E (AAA→GAA)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463480,9150TC99.8% 1867.6 / NA 463K193E (AAA→GAA) PA14_RS0222016S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (239/223);  minor base G (0/1);  total (239/224)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 463 total aligned reads displayed.

TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062
                                                                                                                                                    |                                                                                                                                                   
 atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                                                                  >  2:2713302/1‑151 (MQ=255)
 atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCGACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                                                                  <  2:3175042/151‑1 (MQ=255)
  tCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGa                                                                                                                                                 <  1:6191650/151‑1 (MQ=255)
   cAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAc                                                                                                                                                >  1:8812356/1‑151 (MQ=255)
       cGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccac                                                                                                                                            >  1:10449358/1‑151 (MQ=255)
       cGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccac                                                                                                                                            >  2:1430968/1‑151 (MQ=255)
       cGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccac                                                                                                                                            >  2:3685584/1‑151 (MQ=255)
         aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTTCCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacca                                                                                                                                          <  2:5267286/151‑1 (MQ=255)
         aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacca                                                                                                                                          <  2:4761151/151‑1 (MQ=255)
            tCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGa                                                                                                                                       >  1:10336889/1‑151 (MQ=255)
              gCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAg                                                                                                                                     >  2:6989891/1‑151 (MQ=255)
              gCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAg                                                                                                                                     <  1:6526980/151‑1 (MQ=255)
               ccccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGACgg                                                                                                                                    >  2:8324346/1‑151 (MQ=255)
                cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                                                   <  2:4033958/151‑1 (MQ=255)
                cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                                                   <  1:7965474/151‑1 (MQ=255)
                 ccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGcc                                                                                                                                  >  1:5993170/1‑151 (MQ=255)
                  cAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCa                                                                                                                                 >  2:940753/1‑151 (MQ=255)
                   agagTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAg                                                                                                                                <  1:2906339/151‑1 (MQ=255)
                     agTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTGTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgc                                                                                                                              <  2:4304933/151‑1 (MQ=255)
                      gTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcg                                                                                                                             <  1:2184693/151‑1 (MQ=255)
                              gCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                                                     <  2:3936703/151‑1 (MQ=255)
                               ccACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcgg                                                                                                                    >  1:7861331/1‑151 (MQ=255)
                                 aCCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGca                                                                                                                  <  2:167720/151‑1 (MQ=255)
                                  ccGCCAACGCTACCCCCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGcac                                                                                                                 <  2:6297092/151‑1 (MQ=255)
                                  ccGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGcac                                                                                                                 <  2:959704/151‑1 (MQ=255)
                                    gCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAt                                                                                                               <  1:6312003/151‑1 (MQ=255)
                                     ccAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtg                                                                                                              <  2:5808053/151‑1 (MQ=255)
                                        aCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                                                           >  1:969669/1‑151 (MQ=255)
                                        aCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                                                           <  1:4065101/151‑1 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctgg                                                                                                         >  2:1048486/1‑151 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctgg                                                                                                         >  2:5527396/1‑151 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctgg                                                                                                         <  1:4445949/151‑1 (MQ=255)
                                             accaccGGCGCGGTCTCGGTGCGCAGCACTCTCGGGCCGAGGCGCGCGGCCTGGACGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGACGCCTTCGGTACCCTCCAGGAAGGCACGGCGCCACGGGGGCACAgggctggcga                                                                                                      >  2:6534318/1‑149 (MQ=255)
                                             accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCACCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                                                      <  1:5905226/151‑1 (MQ=255)
                                              ccaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAg                                                                                                     <  2:7514798/151‑1 (MQ=255)
                                                accGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCGAggg                                                                                                   >  2:5839363/1‑151 (MQ=255)
                                                  cGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggggg                                                                                                 >  2:8973266/1‑151 (MQ=255)
                                                  cGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggggg                                                                                                 >  1:945468/1‑151 (MQ=255)
                                                   ggCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgggggg                                                                                                <  2:1604449/151‑1 (MQ=255)
                                                    gcgcGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggc                                                                                               <  1:557132/151‑1 (MQ=255)
                                                       cgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcggc                                                                                            <  1:10713487/151‑1 (MQ=255)
                                                          tctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcac                                                                                         <  1:7135198/151‑1 (MQ=255)
                                                          tctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcac                                                                                         <  2:1757678/151‑1 (MQ=255)
                                                             cGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAgg                                                                                      >  2:4858641/1‑151 (MQ=255)
                                                              ggTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAggg                                                                                     <  1:4321371/151‑1 (MQ=255)
                                                                tGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTg                                                                                   <  1:8501190/151‑1 (MQ=255)
                                                                   gcagcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAg                                                                                >  1:9379553/1‑151 (MQ=255)
                                                                    cagcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAgg                                                                               >  1:5117149/1‑151 (MQ=255)
                                                                     agcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGa                                                                              <  1:1452430/151‑1 (MQ=255)
                                                                          tCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAGGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGc                                                                         >  2:6138871/1‑151 (MQ=255)
                                                                           cgcgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGcc                                                                        <  1:3241296/151‑1 (MQ=255)
                                                                            gcgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCt                                                                       <  2:1198395/151‑1 (MQ=255)
                                                                             cgGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTg                                                                      >  1:6936728/1‑151 (MQ=255)
                                                                                 ccGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCt                                                                  >  1:3305858/1‑151 (MQ=255)
                                                                                   caGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTGCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCGCa                                                                >  2:6030229/2‑151 (MQ=255)
                                                                                    aGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCAt                                                               <  1:2778601/151‑1 (MQ=255)
                                                                                      gcgcgcGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCg                                                             <  2:2146394/151‑1 (MQ=255)
                                                                                         cgcgGCGTGGTAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCCCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCGGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                                                          >  2:7780771/1‑151 (MQ=255)
                                                                                          gcggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTccc                                                         <  1:9051488/151‑1 (MQ=255)
                                                                                           cggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccg                                                        <  1:1561893/151‑1 (MQ=255)
                                                                                            ggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgc                                                       <  1:3838575/151‑1 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     >  2:10672720/1‑151 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     <  1:1337900/151‑1 (MQ=255)
                                                                                                       cggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCa                                            >  2:3133248/1‑151 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           <  2:7380974/151‑1 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           <  1:1889522/151‑1 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           <  1:338668/151‑1 (MQ=255)
                                                                                                         gccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGt                                          >  1:10171751/1‑151 (MQ=255)
                                                                                                         gccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGt                                          >  2:1719277/1‑151 (MQ=255)
                                                                                                             ccTTCGCCTGCTCCACCTCGGCATCGCTAAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctc                                      >  2:8775183/1‑151 (MQ=255)
                                                                                                              cTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctcc                                     >  2:8621479/1‑151 (MQ=255)
                                                                                                               ttcgCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCa                                    >  2:7281082/4‑151 (MQ=255)
                                                                                                                 cgCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAgg                                  >  2:2933774/2‑151 (MQ=255)
                                                                                                                   ccTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGc                                >  2:6436316/1‑151 (MQ=255)
                                                                                                                   ccTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGc                                >  1:2535299/1‑151 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               <  2:8064681/151‑1 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               >  1:8630237/1‑151 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGAGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               >  2:3336874/1‑151 (MQ=255)
                                                                                                                     tGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCa                              <  1:4661055/151‑1 (MQ=255)
                                                                                                                     tGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCa                              >  1:10078520/1‑151 (MQ=255)
                                                                                                                      gCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAg                             >  1:7616089/1‑151 (MQ=255)
                                                                                                                       cTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgc                            <  1:3061384/151‑1 (MQ=255)
                                                                                                                         ccACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcgg                          >  2:9871076/1‑151 (MQ=255)
                                                                                                                          cACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcggc                         >  1:3914347/1‑151 (MQ=255)
                                                                                                                             cTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCgga                      >  2:196415/1‑151 (MQ=255)
                                                                                                                                  cATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatggat                 <  1:4861052/151‑1 (MQ=255)
                                                                                                                                  cATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatggat                 >  2:8463402/1‑151 (MQ=255)
                                                                                                                                   aTCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATc                >  2:10145122/1‑151 (MQ=255)
                                                                                                                                       cTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCg            >  2:5036507/1‑151 (MQ=255)
                                                                                                                                       cTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCg            <  2:300309/151‑1 (MQ=255)
                                                                                                                                        tCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCgg           >  1:1978779/1‑151 (MQ=255)
                                                                                                                                          aGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCa         <  1:9895268/151‑1 (MQ=255)
                                                                                                                                            gccgccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAgg       <  2:4576494/151‑1 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTc    <  1:7345964/151‑1 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTc    <  2:9198179/151‑1 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTc    <  1:3685584/151‑1 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTc    >  2:4346631/1‑151 (MQ=255)
                                                                                                                                                ccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCg   <  1:7548143/151‑1 (MQ=255)
                                                                                                                                                 cTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGa  >  1:5705436/1‑151 (MQ=255)
                                                                                                                                                 cTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGa  <  1:2106116/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑