Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,256,376 T→C F87L (TTC→CTC)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,256,3760TC100.0% 1894.3 / NA 470F87L (TTC→CTC) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (280/190);  total (280/190)

Only 100 of 470 total aligned reads displayed.

GAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAGG  >  NC_008463/5256228‑5256523
                                                                                                                                                    |                                                                                                                                                   
gAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTc                                                                                                                                                   <  2:5875473/151‑1 (MQ=255)
gAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTc                                                                                                                                                   <  1:5044684/151‑1 (MQ=255)
   ggaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                                >  1:1858580/1‑151 (MQ=255)
     aggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgccga                                                                                                                                              >  1:5843646/1‑149 (MQ=255)
      ggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgac                                                                                                                                             <  1:7406339/151‑1 (MQ=255)
        aCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgacaa                                                                                                                                           >  2:8093726/1‑151 (MQ=255)
         cttcttCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCgacaac                                                                                                                                          <  2:5759150/151‑1 (MQ=255)
         cttcttCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCgacaac                                                                                                                                          <  2:7126868/151‑1 (MQ=255)
         cttcttCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCgacaac                                                                                                                                          >  1:8717277/1‑151 (MQ=255)
           tcttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAacac                                                                                                                                        <  2:9643768/151‑1 (MQ=255)
            cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                                       >  2:4605106/1‑151 (MQ=255)
              tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGcc                                                                                                                                     <  2:4243579/151‑1 (MQ=255)
              tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCCCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGcc                                                                                                                                     >  2:3448207/1‑151 (MQ=255)
                 ggCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTc                                                                                                                                  >  1:6267406/1‑151 (MQ=255)
                  gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTcc                                                                                                                                 <  2:4407287/151‑1 (MQ=255)
                   cATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCg                                                                                                                                <  2:1096930/151‑1 (MQ=255)
                    aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                               >  1:9931413/1‑151 (MQ=255)
                    aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                               >  2:9016662/1‑151 (MQ=255)
                    aTGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGt                                                                                                                               <  2:6383981/151‑1 (MQ=255)
                       aGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGc                                                                                                                            <  1:1542542/151‑1 (MQ=255)
                           tCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAat                                                                                                                        >  1:4156052/1‑151 (MQ=255)
                                tGATCTTCGCCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGa                                                                                                                   >  1:365374/1‑151 (MQ=255)
                                tGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGa                                                                                                                   >  2:1293850/1‑151 (MQ=255)
                                 gATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                                  >  1:8593216/1‑151 (MQ=255)
                                 gATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                                  >  2:6034505/1‑151 (MQ=255)
                                  aTCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAgg                                                                                                                 >  2:2832131/1‑151 (MQ=255)
                                   tCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGc                                                                                                                <  1:2351545/151‑1 (MQ=255)
                                     ttCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCtt                                                                                                              >  1:7447681/1‑151 (MQ=255)
                                      tCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTa                                                                                                             >  2:1612028/1‑151 (MQ=255)
                                          cGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgcg                                                                                                         >  2:2095586/1‑151 (MQ=255)
                                           gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                                        >  1:77083/1‑151 (MQ=255)
                                            ccATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCa                                                                                                       >  1:9825081/1‑151 (MQ=255)
                                                cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATc                                                                                                   <  1:6178601/151‑1 (MQ=255)
                                                   cGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGtt                                                                                                <  2:6535742/151‑1 (MQ=255)
                                                    gAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTc                                                                                               >  1:3544619/1‑151 (MQ=255)
                                                     aGTTGGCCGCTAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACATTCCAGTAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                              <  2:3323519/151‑1 (MQ=255)
                                                     aGTTGGCCGCGAATGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                              <  2:478042/151‑1 (MQ=255)
                                                      gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                             >  1:3783337/1‑151 (MQ=255)
                                                            cgcgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCgg                                                                                       <  1:8962227/151‑1 (MQ=255)
                                                               gAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTGCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGca                                                                                    >  1:10052134/1‑151 (MQ=255)
                                                                aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcac                                                                                   >  2:4115725/1‑151 (MQ=255)
                                                                 aGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcaca                                                                                  >  1:1232035/1‑151 (MQ=255)
                                                                     tGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                              <  2:3507130/151‑1 (MQ=255)
                                                                     tGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                              <  2:8474102/151‑1 (MQ=255)
                                                                      gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGc                                                                             >  2:5010281/1‑151 (MQ=255)
                                                                      gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGAGCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGc                                                                             <  2:7402394/151‑1 (MQ=255)
                                                                         ccGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGc                                                                          >  1:8260067/1‑151 (MQ=255)
                                                                          cGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCa                                                                         >  2:8786027/1‑151 (MQ=255)
                                                                            tttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                                       >  1:2147300/1‑151 (MQ=255)
                                                                             ttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCt                                                                      >  1:8395042/1‑151 (MQ=255)
                                                                               gATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGa                                                                    >  1:5315191/1‑151 (MQ=255)
                                                                                aTGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGAt                                                                   >  2:7696336/1‑151 (MQ=255)
                                                                                 tGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATg                                                                  <  2:5592396/151‑1 (MQ=255)
                                                                                  gTCGTGACTGTGTCGGAAGCGATCGAATACCTTACAGAAGCTGGCGGGCTGTCCTACCTCGGCCAGCTCGCCTACTACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                                 >  2:2520774/1‑151 (MQ=255)
                                                                                  gTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                                 >  2:9230620/1‑151 (MQ=255)
                                                                                       gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                            <  2:4001073/151‑1 (MQ=255)
                                                                                       gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                            <  2:2639627/151‑1 (MQ=255)
                                                                                           gtgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAACATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGc                                                        <  2:5147980/151‑1 (MQ=255)
                                                                                            tgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGca                                                       >  1:10561246/1‑151 (MQ=255)
                                                                                             gtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcac                                                      >  2:3095804/1‑151 (MQ=255)
                                                                                               cGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcacca                                                    <  1:2586565/151‑1 (MQ=255)
                                                                                                   aGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGc                                                <  1:3285847/151‑1 (MQ=255)
                                                                                                       aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCa                                            >  1:9619831/1‑151 (MQ=255)
                                                                                                       aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCa                                            >  2:1467898/1‑151 (MQ=255)
                                                                                                        tCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAg                                           <  2:2896787/151‑1 (MQ=255)
                                                                                                          gAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGa                                         >  2:1769061/1‑151 (MQ=255)
                                                                                                          gAAGACCATCCAGAAGCTGGCGGGCTGGCCTACCACCGCCCGCTCGACCACAACACCCCCTCCGTGGCCAAAAACGAGGCATACGCGCAGAACCATCGCGATCGGGCACACCTGCGGCAGCAGAAGTCTCTCGGGCACCACTGCACCAGGa                                         >  1:7541556/1‑151 (MQ=255)
                                                                                                           aaGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                        >  1:6990639/1‑151 (MQ=255)
                                                                                                                cTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCt                                   >  1:6839487/1‑151 (MQ=255)
                                                                                                                cTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCCTGGCCAAAATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCGGATGTCTCTCGGGCACCACTGCACCAGGACCGcca                                   >  1:2669356/1‑150 (MQ=255)
                                                                                                                 ttCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTc                                  <  1:9197517/151‑1 (MQ=255)
                                                                                                                 ttCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTc                                  >  1:2817914/1‑151 (MQ=255)
                                                                                                                     aGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAac                              >  1:5729367/1‑151 (MQ=255)
                                                                                                                       aaGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacca                            >  1:4058872/1‑151 (MQ=255)
                                                                                                                        aGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccac                           <  2:5345955/151‑1 (MQ=255)
                                                                                                                         gCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCCCTGCACCAGGACCGCCTCGAaccacc                          >  2:6758486/1‑151 (MQ=255)
                                                                                                                          cTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAAccacca                         <  1:4223596/151‑1 (MQ=255)
                                                                                                                          cTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAAccacca                         <  2:1657935/151‑1 (MQ=255)
                                                                                                                              cGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCa                     <  1:5395217/151‑1 (MQ=255)
                                                                                                                               gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                    >  1:10231070/1‑151 (MQ=255)
                                                                                                                               gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                    <  2:3844829/151‑1 (MQ=255)
                                                                                                                                 gCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAAt                  <  2:2799726/151‑1 (MQ=255)
                                                                                                                                   tGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATcc                >  1:7924243/1‑151 (MQ=255)
                                                                                                                                   tGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATcc                >  1:4690863/1‑151 (MQ=255)
                                                                                                                                     gCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCct              <  1:5092158/151‑1 (MQ=255)
                                                                                                                                       cTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCctct            >  2:1865214/1‑151 (MQ=255)
                                                                                                                                       cTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCctct            <  1:9399068/151‑1 (MQ=255)
                                                                                                                                         aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa          >  2:10635556/1‑151 (MQ=255)
                                                                                                                                         aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa          <  2:10152527/151‑1 (MQ=255)
                                                                                                                                         aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa          >  2:6155535/1‑151 (MQ=255)
                                                                                                                                         aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa          <  2:942199/151‑1 (MQ=255)
                                                                                                                                         aCCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATAGCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGa          <  2:7821586/151‑1 (MQ=255)
                                                                                                                                          ccTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAg         <  2:2213641/151‑1 (MQ=255)
                                                                                                                                          ccTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAg         <  1:1715662/151‑1 (MQ=255)
                                                                                                                                           cTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAgg        <  2:6895517/151‑1 (MQ=255)
                                                                                                                                           cTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATACCTCTGAgg        <  1:5945368/151‑1 (MQ=255)
                                                                                                                                            tCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt       >  1:7167366/1‑151 (MQ=255)
                                                                                                                                             cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGtt      <  2:10380603/151‑1 (MQ=255)
                                                                                                                                               gCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACAACTGCGGCATCTGATGTCTCTCGGTCACCACTGCACCAGGAACGCCTCGAACCAACAGGCAAATCCCTCTGAGGTTCa    <  1:5491326/151‑1 (MQ=255)
                                                                                                                                                 cAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCagg  >  2:9529106/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAGG  >  NC_008463/5256228‑5256523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑