Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,968,0250CG100.0% 1825.0 / NA 454P426A (CCC→GCC) phaCclass II poly(R)‑hydroxyalkanoic acid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (248/206);  total (248/206)

Only 100 of 459 total aligned reads displayed.

GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172
                                                                                                                                                    |                                                                                                                                                   
  actactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCt                                                                                                                                                 <  1:629215/151‑1 (MQ=255)
  actactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCt                                                                                                                                                 >  1:224698/1‑151 (MQ=255)
   ctactGTGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                <  2:9190371/151‑1 (MQ=255)
     actGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGa                                                                                                                                              >  1:10133712/1‑151 (MQ=255)
      ctGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                             >  1:963368/1‑151 (MQ=255)
      ctGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                             <  1:5144840/151‑1 (MQ=255)
      ctGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                             <  2:10407832/151‑1 (MQ=255)
       tGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAgg                                                                                                                                            >  1:9320104/1‑151 (MQ=255)
         ggTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGtc                                                                                                                                          <  1:3307219/151‑1 (MQ=255)
             aacaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCg                                                                                                                                      <  2:76311/151‑1 (MQ=255)
             aacaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCg                                                                                                                                      >  1:2908753/1‑151 (MQ=255)
               caacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGGTCAAGAGCACCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGc                                                                                                                                    >  2:3591630/1‑151 (MQ=255)
                aacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                   >  1:1746756/1‑151 (MQ=255)
                   tACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGc                                                                                                                                >  2:2629667/1‑151 (MQ=255)
                     ccTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGccc                                                                                                                              >  1:9416949/1‑151 (MQ=255)
                      cTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCa                                                                                                                             >  2:905489/1‑151 (MQ=255)
                      cTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCa                                                                                                                             <  1:4938268/151‑1 (MQ=255)
                        gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           <  1:8433225/151‑1 (MQ=255)
                        gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           >  2:9606419/1‑151 (MQ=255)
                         cTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCg                                                                                                                          >  2:683100/1‑151 (MQ=255)
                            ggCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGAcc                                                                                                                       <  2:10239295/151‑1 (MQ=255)
                                 ccAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAg                                                                                                                  >  2:6675304/1‑151 (MQ=255)
                                  cAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGc                                                                                                                 >  1:10068376/1‑151 (MQ=255)
                                   aGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCa                                                                                                                <  1:594796/151‑1 (MQ=255)
                                   aGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCa                                                                                                                >  2:2145279/1‑151 (MQ=255)
                                      cgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGt                                                                                                             <  1:2432973/151‑1 (MQ=255)
                                        ccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGa                                                                                                           >  2:5014366/1‑151 (MQ=255)
                                        ccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGa                                                                                                           >  2:1569198/1‑151 (MQ=255)
                                            cGTTCGACATCCTCTACTGGAACACCGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                       <  2:5255368/151‑1 (MQ=255)
                                            cGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCTCGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCGGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                       >  1:8724010/1‑151 (MQ=255)
                                             gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                      >  1:2841319/1‑151 (MQ=255)
                                             gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                      >  2:7041253/1‑151 (MQ=255)
                                              ttCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCg                                                                                                     <  1:3608951/151‑1 (MQ=255)
                                                cGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGAc                                                                                                   <  1:7579468/151‑1 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  >  2:2904849/1‑151 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  <  1:4129296/151‑1 (MQ=255)
                                                   cATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCTGGTGACTTGCGACTTc                                                                                                >  2:5888751/1‑151 (MQ=255)
                                                     tCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTa                                                                                              <  2:6437304/151‑1 (MQ=255)
                                                       ctctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACt                                                                                            <  2:7331358/151‑1 (MQ=255)
                                                         ctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACtgt                                                                                          >  2:10525490/1‑151 (MQ=255)
                                                          tACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACtgtg                                                                                         <  2:1215017/151‑1 (MQ=255)
                                                           aCTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTgtgt                                                                                        >  1:452127/1‑151 (MQ=255)
                                                            cTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTc                                                                                       <  1:5841901/151‑1 (MQ=255)
                                                            cTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTc                                                                                       <  1:1511451/151‑1 (MQ=255)
                                                            cTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGAGTc                                                                                       >  2:2720638/1‑151 (MQ=255)
                                                               gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGcc                                                                                    >  2:2338048/1‑151 (MQ=255)
                                                                aacaacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCTGGTGACTTGCGACTTCTACTGTGTCGCCg                                                                                   >  2:5384151/1‑151 (MQ=255)
                                                                aacaacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCg                                                                                   >  2:6188967/1‑151 (MQ=255)
                                                                   aacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGtg                                                                                <  2:4834876/151‑2 (MQ=255)
                                                                   aacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTc                                                                                >  2:3136211/1‑151 (MQ=255)
                                                                   aacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTc                                                                                <  2:9336809/151‑1 (MQ=255)
                                                                    acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                               <  2:3405372/151‑1 (MQ=255)
                                                                    acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                               <  1:1328070/151‑1 (MQ=255)
                                                                      gACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGa                                                                             >  2:1693290/1‑151 (MQ=255)
                                                                      gACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTACGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGCTCTGa                                                                             <  1:383508/151‑1 (MQ=255)
                                                                         accacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACg                                                                          >  2:8825911/1‑151 (MQ=255)
                                                                           cacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAc                                                                        <  1:4318922/151‑1 (MQ=255)
                                                                              gcgcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACcac                                                                     <  1:7692597/151‑1 (MQ=255)
                                                                                  cTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCa                                                                 <  1:3152095/151‑1 (MQ=255)
                                                                                   tGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                                <  1:3462386/151‑1 (MQ=255)
                                                                                    gCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAcc                                                               <  2:3822523/151‑1 (MQ=255)
                                                                                     cccGCCGCGCTGCACGGCGAGTTCGTCGAACTGTGCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGTCTTGCGACTTCTTCTGTGTCGCCGGTCTGAACGACCACATCAccc                                                              >  2:6005694/1‑151 (MQ=255)
                                                                                      ccgccgCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAcccc                                                             >  2:1900452/1‑151 (MQ=255)
                                                                                       cgccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAccccc                                                            <  2:7761077/151‑1 (MQ=255)
                                                                                        gccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                           >  1:4834189/1‑151 (MQ=255)
                                                                                         ccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                          <  1:2743481/151‑1 (MQ=255)
                                                                                          cgcgCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACGTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTgg                                                         >  2:5803221/1‑151 (MQ=255)
                                                                                              cTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGt                                                     >  1:8645242/1‑151 (MQ=255)
                                                                                               tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTc                                                    <  1:7235799/151‑1 (MQ=255)
                                                                                                 cACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGt                                                  <  2:9250564/151‑1 (MQ=255)
                                                                                                       gAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACa                                            >  1:1123668/1‑151 (MQ=255)
                                                                                                         gTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAg                                          >  2:3330850/1‑151 (MQ=255)
                                                                                                            cgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                       <  2:2223432/151‑1 (MQ=255)
                                                                                                            cgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCAGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                       <  2:8947947/151‑1 (MQ=255)
                                                                                                             gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCgg                                      >  2:28163/1‑151 (MQ=255)
                                                                                                             gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCgg                                      >  2:8213299/1‑151 (MQ=255)
                                                                                                              tcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGc                                     >  2:8604850/1‑151 (MQ=255)
                                                                                                               cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGcc                                    >  1:6929755/1‑151 (MQ=255)
                                                                                                               cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGcc                                    >  1:8173458/1‑151 (MQ=255)
                                                                                                                gAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                   >  2:3532391/1‑151 (MQ=255)
                                                                                                                gAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                   >  1:9063883/1‑151 (MQ=255)
                                                                                                                  aCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAgg                                 >  2:4639419/1‑151 (MQ=255)
                                                                                                                    tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCt                               <  1:5435375/151‑1 (MQ=255)
                                                                                                                    tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCt                               <  1:7041253/151‑1 (MQ=255)
                                                                                                                         aaGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTgg                          >  2:8551076/1‑151 (MQ=255)
                                                                                                                           gagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggc                        <  1:6419371/151‑1 (MQ=255)
                                                                                                                                     cTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCg              <  1:5656720/151‑1 (MQ=255)
                                                                                                                                      tGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGa             >  1:8984785/1‑151 (MQ=255)
                                                                                                                                       gAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAg            <  2:41842/151‑1 (MQ=255)
                                                                                                                                        aaCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt           >  1:8534602/1‑151 (MQ=255)
                                                                                                                                           cGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCa        >  1:2156948/1‑151 (MQ=255)
                                                                                                                                           cGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCa        <  1:5540954/151‑1 (MQ=255)
                                                                                                                                            gCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCAt       >  2:8070958/1‑151 (MQ=255)
                                                                                                                                            gCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCAt       <  1:3974614/151‑1 (MQ=255)
                                                                                                                                            gCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACGTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAGGTGCGAGTTCAt       >  2:5532316/1‑151 (MQ=255)
                                                                                                                                            gCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAGCGAGGTGAAGCAGGTGACTTGCGACTGCTCCTGTGTCGACGGTGTGTACGAAGACATGTCCCGCTGGGAGTCGTGCTCGGAGTCGGCGAGGCTACTGGGCGGCAAGTGCGTGGTCAt       >  2:7934179/1‑151 (MQ=255)
                                                                                                                                             ccccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGGTCATc      >  2:715186/1‑151 (MQ=255)
                                                                                                                                                cGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctc   <  1:3000599/151‑1 (MQ=255)
                                                                                                                                                cGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctc   <  2:9791548/151‑1 (MQ=255)
                                                                                                                                                 ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctct  <  1:1063619/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑