Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,076,066 G→C R105R (CGG→CGC)  PA14_RS27775 → methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,076,0660GC99.6% 1940.6 / ‑4.1 499R105R (CGG→CGC) PA14_RS27775methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (242/254);  minor base A (1/1);  total (243/256)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 499 total aligned reads displayed.

GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213
                                                                                                                                                    |                                                                                                                                                   
 gcgcAGCCTCGCACAGGCCGACGCTGCGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCGGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTc                                                                                                                                                  <  2:9020210/151‑1 (MQ=255)
   gcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCaa                                                                                                                                                <  1:8239223/151‑1 (MQ=255)
    cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCCTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                               >  2:9436158/1‑151 (MQ=255)
    cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                               >  2:4059367/1‑151 (MQ=255)
      gCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                             <  2:4551245/151‑1 (MQ=255)
      gCCTCGAACACGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                             <  1:2262282/151‑1 (MQ=255)
         tCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGa                                                                                                                                          <  1:4937149/151‑1 (MQ=255)
           gAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACg                                                                                                                                        <  2:2381595/151‑1 (MQ=255)
           gAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGGCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACg                                                                                                                                        >  2:2731985/1‑151 (MQ=255)
            aaCAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGt                                                                                                                                       <  1:1930352/151‑1 (MQ=255)
                ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCg                                                                                                                                   <  2:5282889/151‑1 (MQ=255)
                     aCGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACg                                                                                                                              >  1:6880782/1‑151 (MQ=255)
                          gAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGt                                                                                                                         >  1:4699461/1‑151 (MQ=255)
                           aGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTg                                                                                                                        >  1:7462740/1‑151 (MQ=255)
                             cacaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgc                                                                                                                      <  1:3073633/151‑1 (MQ=255)
                             cacaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgc                                                                                                                      <  2:6499967/151‑1 (MQ=255)
                              acaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgca                                                                                                                     >  1:4243300/1‑151 (MQ=255)
                                aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcagc                                                                                                                   >  2:7631429/1‑151 (MQ=255)
                                aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTAACCGCAGCTCGCCGCCCAGGCCGAAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTCgcagc                                                                                                                   >  2:9620825/1‑151 (MQ=255)
                                         aCCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGcc                                                                                                          <  1:3721985/151‑1 (MQ=255)
                                         aCCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGcc                                                                                                          >  1:9510256/1‑151 (MQ=255)
                                          cccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCt                                                                                                         <  1:6507692/151‑1 (MQ=255)
                                           ccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTg                                                                                                        >  1:5466604/1‑151 (MQ=255)
                                             gCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGtt                                                                                                      >  2:9248142/1‑151 (MQ=255)
                                              ccTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTc                                                                                                     <  2:3146072/151‑1 (MQ=255)
                                                 ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                  <  1:5134608/151‑1 (MQ=255)
                                                 ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGcc                                                                                                  >  1:1124793/1‑151 (MQ=255)
                                                        cccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGcc                                                                                           >  1:3901065/1‑151 (MQ=255)
                                                         ccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCt                                                                                          <  2:5940077/151‑1 (MQ=255)
                                                            cgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCa                                                                                       <  2:1418107/151‑1 (MQ=255)
                                                            cgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCa                                                                                       <  1:5036096/151‑1 (MQ=255)
                                                             gccTGATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGAGCGGCCTGTTCGCCGGGCGCCTGCAg                                                                                      >  2:6905106/1‑151 (MQ=255)
                                                              ccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCTGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGt                                                                                     >  2:9695469/1‑151 (MQ=255)
                                                               cTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGtt                                                                                    >  2:7106480/1‑151 (MQ=255)
                                                                 tATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCg                                                                                  >  2:876310/1‑151 (MQ=255)
                                                                    ccGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGccc                                                                               <  2:8562115/151‑1 (MQ=255)
                                                                    ccGCAGCTCGCCGCCCAGGCCGCAGCGCGGGGCGCGGTCGGCGACCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGccc                                                                               <  1:5706771/151‑1 (MQ=255)
                                                                         gcgcgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccg                                                                          <  2:434532/148‑1 (MQ=255)
                                                                         gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccg                                                                          <  1:9773763/151‑1 (MQ=255)
                                                                         gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccg                                                                          >  2:10177772/1‑151 (MQ=255)
                                                                         gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccg                                                                          >  2:3519245/1‑151 (MQ=255)
                                                                          cTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCCATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccgg                                                                         >  1:2969961/1‑151 (MQ=255)
                                                                            cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtc                                                                       <  1:5926308/151‑1 (MQ=255)
                                                                             gccgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcg                                                                      <  2:8358767/151‑1 (MQ=255)
                                                                             gccgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATGGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcg                                                                      >  2:8427865/1‑151 (MQ=255)
                                                                               cgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgtc                                                                    >  2:7471570/1‑151 (MQ=255)
                                                                               cgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgtc                                                                    >  2:2115501/1‑151 (MQ=255)
                                                                                gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                   >  2:7831926/1‑151 (MQ=255)
                                                                                gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                   >  1:4485270/1‑151 (MQ=255)
                                                                                 cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCa                                                                  >  1:8569866/1‑151 (MQ=255)
                                                                                    aGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTg                                                               >  1:6136535/1‑151 (MQ=255)
                                                                                       ccGCAGCGCTGGGCGCGGCCGGCGCACAGCCATTGGCTGAACGCCCGCAGGCACCGACCCCCTTCAATCTCGCCGTACCGTGACGCCAGAGGCATCGGATCGGCCTGGTCGCTGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCt                                                            <  2:7404830/151‑1 (MQ=255)
                                                                                        cgccgcGCTGGGCGCGGTCGGCGAACAGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAGGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGACGTCCACCGGCTc                                                           <  1:3201313/151‑1 (MQ=255)
                                                                                        cGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTc                                                           >  2:4898017/1‑151 (MQ=255)
                                                                                           aGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGAc                                                        >  1:8485546/1‑151 (MQ=255)
                                                                                            gcgcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACt                                                       <  2:1254947/151‑1 (MQ=255)
                                                                                               cTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGt                                                    <  1:3440016/151‑1 (MQ=255)
                                                                                                tGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                   >  1:704108/1‑151 (MQ=255)
                                                                                                tGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                   <  2:9856953/151‑1 (MQ=255)
                                                                                                 gggCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGc                                                  >  1:7687380/1‑151 (MQ=255)
                                                                                                        gTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCa                                           <  2:10597050/151‑1 (MQ=255)
                                                                                                          cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAg                                         >  2:2901856/1‑151 (MQ=255)
                                                                                                          cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAg                                         >  2:10336156/1‑151 (MQ=255)
                                                                                                           ggCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                        >  2:1600271/1‑151 (MQ=255)
                                                                                                           ggCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                        >  1:5802681/1‑151 (MQ=255)
                                                                                                            gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAggg                                       >  1:10058878/1‑151 (MQ=255)
                                                                                                             cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGc                                      >  2:6297993/1‑151 (MQ=255)
                                                                                                             cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGc                                      >  2:8195036/1‑151 (MQ=255)
                                                                                                              gAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                     <  2:6789038/151‑1 (MQ=255)
                                                                                                               aaCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCa                                    >  2:1932988/1‑151 (MQ=255)
                                                                                                                aCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCAt                                   >  1:10298876/1‑151 (MQ=255)
                                                                                                                 cTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGAAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATc                                  >  2:10236259/1‑151 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 <  1:2784986/151‑1 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 <  2:8669285/151‑1 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 >  1:7317659/1‑151 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 <  2:6034984/151‑1 (MQ=255)
                                                                                                                  tGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCt                                 <  1:9370083/151‑1 (MQ=255)
                                                                                                                   gCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTg                                <  1:1339168/151‑1 (MQ=255)
                                                                                                                   gCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTg                                >  2:1696517/1‑151 (MQ=255)
                                                                                                                    ccATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTgg                               <  2:5592139/151‑1 (MQ=255)
                                                                                                                       ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgcc                            <  2:372059/151‑1 (MQ=255)
                                                                                                                       ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgcc                            >  1:172191/1‑151 (MQ=255)
                                                                                                                       ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgcc                            <  1:3856498/151‑1 (MQ=255)
                                                                                                                       ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgcc                            >  2:5371979/1‑151 (MQ=255)
                                                                                                                            ggAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                       <  1:1765373/151‑1 (MQ=255)
                                                                                                                            ggAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                       <  1:6318647/151‑1 (MQ=255)
                                                                                                                              aaCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgct                     <  1:4831877/151‑1 (MQ=255)
                                                                                                                               aCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgctg                    <  1:10587798/151‑1 (MQ=255)
                                                                                                                                     gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCAt              <  1:8877682/151‑1 (MQ=255)
                                                                                                                                      cAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTTGGCCGCCTGCTGGCCCATg             <  2:1171023/151‑1 (MQ=255)
                                                                                                                                       aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc            >  1:7901079/1‑151 (MQ=255)
                                                                                                                                       aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGc            >  1:2554551/1‑151 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         <  2:7954342/151‑1 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         >  1:4064143/1‑151 (MQ=255)
                                                                                                                                          cACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCggg         <  1:8031771/151‑1 (MQ=255)
                                                                                                                                           aCCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCgggg        <  1:914103/151‑1 (MQ=255)
                                                                                                                                            ccGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGc       >  2:9136555/1‑151 (MQ=255)
                                                                                                                                                aCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAcc   <  2:8049663/151‑1 (MQ=255)
                                                                                                                                                 ccGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCg  >  2:5190373/1‑151 (MQ=255)
                                                                                                                                                 ccGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCg  <  1:10355645/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACCG  >  NC_008463/6075918‑6076213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑