Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846372,4400TC99.8% 1727.6 / NA 432K132E (AAG→GAG) PA14_RS00315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (178/253);  minor base A (1/0);  total (179/253)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587
                                                                                                                                                    |                                                                                                                                                   
 ggCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTcgcg                                                                                                                                                  >  1:1392258/1‑151 (MQ=255)
  gCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCgg                                                                                                                                                 <  1:1725783/151‑1 (MQ=255)
  gCACCGGTCCGGCGCAGCCGGCGAGGAGCCGCCGCGCGAGCGGGACGCGACGGCAGCGTGGCCGCAAGGGGCGGGCTCCGGACGGCGTACAGCACCCAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCCGCGCCCCTCGCgg                                                                                                                                                 <  2:8131405/151‑1 (MQ=255)
   cAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGc                                                                                                                                                <  1:26164/151‑1 (MQ=255)
     gCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcg                                                                                                                                              <  2:299407/151‑1 (MQ=255)
          ccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCgcgcgc                                                                                                                                         <  1:10608817/151‑1 (MQ=255)
          ccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCgcgcgc                                                                                                                                         <  2:8253869/151‑1 (MQ=255)
           cGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGcc                                                                                                                                        <  2:4807577/151‑1 (MQ=255)
            ggCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCa                                                                                                                                       >  1:6803325/1‑151 (MQ=255)
            ggCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCa                                                                                                                                       <  1:2747881/151‑1 (MQ=255)
             gcgcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCaa                                                                                                                                      <  1:7232953/151‑1 (MQ=255)
              cgcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAt                                                                                                                                     <  2:392138/151‑1 (MQ=255)
                cAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTc                                                                                                                                   <  1:1561169/151‑1 (MQ=255)
                    cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtg                                                                                                                               >  1:8762387/1‑151 (MQ=255)
                    cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtg                                                                                                                               <  2:853816/151‑1 (MQ=255)
                      gCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgtt                                                                                                                             <  2:7324735/151‑1 (MQ=255)
                       cGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttg                                                                                                                            >  1:5766446/1‑151 (MQ=255)
                       cGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttg                                                                                                                            >  2:8642416/1‑151 (MQ=255)
                        gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttgt                                                                                                                           >  2:5625453/1‑151 (MQ=255)
                         aggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTc                                                                                                                          >  1:2386506/1‑151 (MQ=255)
                              cagcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGt                                                                                                                     <  2:6784201/151‑1 (MQ=255)
                              cagcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGt                                                                                                                     <  2:7028499/151‑1 (MQ=255)
                                    cgcgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTaga                                                                                                               <  1:39690/151‑1 (MQ=255)
                                       gAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCt                                                                                                            <  1:7357890/151‑1 (MQ=255)
                                       gAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCt                                                                                                            >  1:5917659/1‑151 (MQ=255)
                                          cGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCg                                                                                                         <  2:9259244/151‑1 (MQ=255)
                                          cGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCg                                                                                                         >  2:2889777/1‑151 (MQ=255)
                                               cGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAg                                                                                                    >  2:7803833/1‑151 (MQ=255)
                                                 agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCGGCTGCTAGCATAGCGCCCTCGCCTTGCGCGGTTCAGACCTTCGGGCCGCGCCCCTCGCGGCAGCGCGCCACGTCCGTGTTGTGCTGGTCGTATAGCTTCGGCCTggg                                                                                                  >  2:5406304/1‑151 (MQ=255)
                                                 agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                  >  1:773064/1‑151 (MQ=255)
                                                    cGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                               <  1:8542962/151‑1 (MQ=255)
                                                     gggTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACgg                                                                                              >  2:1633996/1‑151 (MQ=255)
                                                     gggTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACgg                                                                                              >  2:1536545/1‑151 (MQ=255)
                                                       gTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGaa                                                                                            >  1:3236968/1‑151 (MQ=255)
                                                        tGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATCGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                           <  1:3760255/151‑1 (MQ=255)
                                                          aGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                         >  1:9229639/1‑151 (MQ=255)
                                                            tGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGa                                                                                       <  2:2808381/151‑1 (MQ=255)
                                                             ggACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAt                                                                                      >  1:9609067/1‑151 (MQ=255)
                                                              gACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtg                                                                                     <  1:4650990/151‑1 (MQ=255)
                                                               aCGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtgt                                                                                    <  1:10141688/151‑1 (MQ=255)
                                                                 gCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGc                                                                                  >  1:10005889/1‑151 (MQ=255)
                                                                  cATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGcc                                                                                 >  1:759852/1‑151 (MQ=255)
                                                                  cATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGcc                                                                                 <  2:222302/151‑1 (MQ=255)
                                                                    tGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCg                                                                               <  2:6309647/151‑1 (MQ=255)
                                                                     ggggCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCgg                                                                              <  2:4547247/151‑1 (MQ=255)
                                                                       ggcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGcc                                                                            >  1:7059539/1‑151 (MQ=255)
                                                                          gggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTcc                                                                         >  2:7704964/1‑151 (MQ=255)
                                                                          gggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTcc                                                                         <  1:9393507/151‑1 (MQ=255)
                                                                             cTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                      <  2:9793515/151‑1 (MQ=255)
                                                                              accGGAGGGCGTACAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGc                                                                     <  2:7182954/150‑1 (MQ=255)
                                                                                cGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCgg                                                                   >  2:5195852/1‑151 (MQ=255)
                                                                                 ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                  <  1:3110703/151‑1 (MQ=255)
                                                                                  gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTa                                                                 >  1:5237636/1‑151 (MQ=255)
                                                                                  gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTa                                                                 <  2:2520479/151‑1 (MQ=255)
                                                                                   aGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                                <  2:8593816/151‑1 (MQ=255)
                                                                                    gggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgc                                                               <  2:9023600/151‑1 (MQ=255)
                                                                                     ggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgcg                                                              >  1:4965333/1‑151 (MQ=255)
                                                                                        gTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTg                                                           >  1:4852260/1‑151 (MQ=255)
                                                                                          tCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                         <  1:9737191/151‑1 (MQ=255)
                                                                                          tCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                         >  1:1933330/1‑151 (MQ=255)
                                                                                            aGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCg                                                       >  1:7436458/1‑151 (MQ=255)
                                                                                             gCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGGCGGAAGCCGATGTGCCCGGCCGCCCGGCGGTCGCGCTGGCCgg                                                      >  2:5703638/1‑151 (MQ=255)
                                                                                             gCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                      <  2:10216996/151‑1 (MQ=255)
                                                                                              cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGNCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgc                                                     <  1:5624993/151‑1 (MQ=255)
                                                                                              cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgc                                                     <  1:890081/151‑1 (MQ=255)
                                                                                                   aGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCCGGCGGTCGCGCTGGCCGGCGCTGa                                                >  2:7457857/1‑151 (MQ=255)
                                                                                                   aGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGa                                                >  1:1862606/1‑151 (MQ=255)
                                                                                                    gCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGaa                                               <  1:6300969/151‑1 (MQ=255)
                                                                                                      aTAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAGTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGt                                             <  2:10564401/151‑1 (MQ=255)
                                                                                                       tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTc                                            >  1:10462721/1‑151 (MQ=255)
                                                                                                       tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTc                                            <  1:6930811/151‑1 (MQ=255)
                                                                                                        aGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTcc                                           <  1:9105908/151‑1 (MQ=255)
                                                                                                          cgcCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGACGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCtt                                         <  2:8371030/151‑1 (MQ=255)
                                                                                                           gcCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTg                                        <  1:7206253/151‑1 (MQ=255)
                                                                                                            cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                       >  2:10376693/1‑151 (MQ=255)
                                                                                                             ccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGaa                                      <  1:9050695/151‑1 (MQ=255)
                                                                                                              cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAAc                                     <  2:4141745/151‑1 (MQ=255)
                                                                                                               tCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACt                                    >  1:6497497/1‑151 (MQ=255)
                                                                                                               tCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACt                                    <  1:2868198/151‑1 (MQ=255)
                                                                                                                 gCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTg                                  >  1:214055/1‑151 (MQ=255)
                                                                                                                  ccTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                 <  1:4250518/151‑1 (MQ=255)
                                                                                                                     tGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGt                              <  1:9896775/151‑1 (MQ=255)
                                                                                                                     tGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGGTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGt                              <  2:3194446/151‑1 (MQ=255)
                                                                                                                      gcgcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGtt                             <  2:3255544/151‑1 (MQ=255)
                                                                                                                      gcgcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGtt                             <  2:9192507/151‑1 (MQ=255)
                                                                                                                         ccGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAg                          <  2:203936/151‑1 (MQ=255)
                                                                                                                         ccGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAg                          <  1:8281394/151‑1 (MQ=255)
                                                                                                                           gTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCt                        >  1:8167828/1‑151 (MQ=255)
                                                                                                                            ttCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTg                       <  2:956416/151‑1 (MQ=255)
                                                                                                                               aGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCa                    <  1:1300502/151‑1 (MQ=255)
                                                                                                                               aGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCa                    <  2:8122069/151‑1 (MQ=255)
                                                                                                                                  ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGATGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCGGGCCGGCGCTGAAGTCCTTGACCTTGAGGTTGAGCTGGCACTc                 >  2:6613803/1‑151 (MQ=255)
                                                                                                                                  ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATc                 <  1:3514273/151‑1 (MQ=255)
                                                                                                                                  ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATc                 <  1:6622972/151‑1 (MQ=255)
                                                                                                                                   cTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcg                <  1:8145236/151‑1 (MQ=255)
                                                                                                                                   cTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATcg                <  2:4710661/151‑1 (MQ=255)
                                                                                                                                      cgcgCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCgcgc             >  1:7873456/1‑151 (MQ=255)
                                                                                                                                                cccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc   <  2:131092/151‑1 (MQ=255)
                                                                                                                                                cccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc   <  2:3423903/151‑1 (MQ=255)
                                                                                                                                                cccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc   <  2:352044/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGCT  >  NC_008463/72292‑72587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑