Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 982,940 A→G F293F (TTT→TTC)  PA14_RS04570 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463982,9400AG99.8% 1766.9 / NA 446F293F (TTT→TTC) PA14_RS04570ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (217/228);  minor base T (0/1);  total (217/229)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 456 total aligned reads displayed.

CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087
                                                                                                                                                    |                                                                                                                                                   
ccTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCCCCGATCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACgaa                                                                                                                                                   <  2:3799868/151‑1 (MQ=255)
   cGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGACCAGCGAGACCACGAACAg                                                                                                                                                >  2:891684/1‑151 (MQ=255)
   cGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAg                                                                                                                                                >  1:4026955/1‑151 (MQ=255)
     gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              <  2:8421706/151‑1 (MQ=255)
         gcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCtt                                                                                                                                          <  2:957637/151‑1 (MQ=255)
            gcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtctc                                                                                                                                       <  1:7480643/151‑1 (MQ=255)
             cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                      >  1:4765141/1‑151 (MQ=255)
                  cGAAGATGTGCCAGGGGCGCGCGCCCCCCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGt                                                                                                                                 <  2:7362317/151‑1 (MQ=255)
                   gAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTg                                                                                                                                >  2:9094468/1‑151 (MQ=255)
                   gAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGGTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGCCGAACTGCGAGACCACGAACAGTGCCTTCGCGGCGGTg                                                                                                                                >  2:272517/1‑151 (MQ=255)
                      gATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGccc                                                                                                                             >  2:9138183/1‑151 (MQ=255)
                           gCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAg                                                                                                                        >  2:2128790/1‑151 (MQ=255)
                                    gcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAg                                                                                                               <  1:9654260/151‑1 (MQ=255)
                                        gcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTcc                                                                                                           >  2:6522685/1‑151 (MQ=255)
                                        gcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTcc                                                                                                           <  2:2272139/151‑1 (MQ=255)
                                         cccACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCt                                                                                                          >  2:7921981/1‑151 (MQ=255)
                                           cACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGc                                                                                                        >  2:399185/1‑151 (MQ=255)
                                                aGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGc                                                                                                   <  1:10185415/151‑1 (MQ=255)
                                                  cgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGa                                                                                                 >  2:4962879/1‑151 (MQ=255)
                                                    cAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGaca                                                                                               >  2:622045/1‑151 (MQ=255)
                                                       gATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCg                                                                                            <  2:9006882/151‑1 (MQ=255)
                                                                ctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATc                                                                                   >  1:5109503/1‑151 (MQ=255)
                                                                  cccGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGc                                                                                 <  1:4476971/151‑1 (MQ=255)
                                                                  cccGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGGGAGGACGCCGAACAGCGAGACCACGCACAGTGCCTTCGCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGc                                                                                 >  2:6393831/1‑151 (MQ=255)
                                                                   ccGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCAAGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGAGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGcc                                                                                >  2:2948422/1‑151 (MQ=255)
                                                                     ggcggcGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCAt                                                                              >  1:10456798/1‑151 (MQ=255)
                                                                      gcggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATc                                                                             >  2:9692112/1‑151 (MQ=255)
                                                                      gcggcgCTAGTTGAGGCTGGTGAGGATCGCCGTGCTCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATc                                                                             <  2:5353836/151‑1 (MQ=255)
                                                                       cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCa                                                                            >  1:3622449/1‑151 (MQ=255)
                                                                       cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCa                                                                            <  1:1132280/151‑1 (MQ=255)
                                                                       cggcgCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCa                                                                            <  2:5981733/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  2:4473164/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  2:9843942/151‑1 (MQ=255)
                                                                          cgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCg                                                                         <  2:7074146/151‑1 (MQ=255)
                                                                          cgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCg                                                                         >  2:9308087/1‑151 (MQ=255)
                                                                          cgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCg                                                                         >  1:671871/1‑151 (MQ=255)
                                                                           gcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                        <  1:492174/151‑1 (MQ=255)
                                                                             tCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGtt                                                                      >  1:2059397/1‑151 (MQ=255)
                                                                             gcGTTGAGGCGGGTGAGGTTCGGCGTGAGCAGGCGGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGACCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGtt                                                                      <  2:1193129/150‑1 (MQ=255)
                                                                              cGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTc                                                                     <  2:5409667/151‑1 (MQ=255)
                                                                                  gAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCg                                                                 >  1:7746532/1‑151 (MQ=255)
                                                                                     gCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGa                                                              <  1:7899472/151‑1 (MQ=255)
                                                                                     gCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGa                                                              <  1:8555696/151‑1 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                             >  1:5335460/1‑151 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                             <  1:1683129/151‑1 (MQ=255)
                                                                                        ggTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAg                                                           >  1:9133414/1‑151 (MQ=255)
                                                                                          tGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTc                                                         >  1:3205970/1‑151 (MQ=255)
                                                                                               aTCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGa                                                    <  1:6269292/151‑1 (MQ=255)
                                                                                                  gCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGTCACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                 <  2:10586568/151‑1 (MQ=255)
                                                                                                  gCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                 >  1:8428196/1‑151 (MQ=255)
                                                                                                    cGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcg                                               <  2:6920522/151‑1 (MQ=255)
                                                                                                     gTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgc                                              >  2:334362/1‑151 (MQ=255)
                                                                                                     gTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgc                                              >  1:3484813/1‑151 (MQ=255)
                                                                                                     gTGAGCAAGCCGCTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCGTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgc                                              <  2:6636849/151‑1 (MQ=255)
                                                                                                      tGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgcg                                             >  1:9402803/1‑151 (MQ=255)
                                                                                                        aGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                           <  2:1515746/151‑1 (MQ=255)
                                                                                                        aGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGtt                                           <  2:10710579/151‑1 (MQ=255)
                                                                                                            tGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAg                                       >  1:2772435/1‑151 (MQ=255)
                                                                                                              ccGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAggg                                     <  2:1366596/151‑1 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    >  2:6797294/1‑151 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    >  2:4135301/1‑151 (MQ=255)
                                                                                                                   cAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAg                                <  1:10013720/151‑1 (MQ=255)
                                                                                                                     ggCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                              <  2:2449753/151‑1 (MQ=255)
                                                                                                                       ccGGTGAGGACGACGAACAGCGAGACCACGACCAGTGCCTTCTCGGCGGTGCCCATCAGGCCACCAAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATTGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCg                            <  2:10140152/151‑1 (MQ=255)
                                                                                                                        cGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGc                           >  1:8940609/1‑151 (MQ=255)
                                                                                                                        cGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGc                           >  2:4755559/1‑151 (MQ=255)
                                                                                                                        cGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGc                           <  2:5640582/151‑1 (MQ=255)
                                                                                                                         ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGcc                          >  1:6101093/1‑151 (MQ=255)
                                                                                                                          gTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                         >  1:9537094/1‑151 (MQ=255)
                                                                                                                          gTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                         <  2:10328062/151‑1 (MQ=255)
                                                                                                                           tGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAc                        >  1:5185231/1‑151 (MQ=255)
                                                                                                                            gAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAcc                       >  1:9844476/1‑151 (MQ=255)
                                                                                                                             aGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCt                      <  2:3022282/151‑1 (MQ=255)
                                                                                                                              ggACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCtt                     >  1:5838298/1‑151 (MQ=255)
                                                                                                                               gacgacGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTg                    <  1:5465172/151‑1 (MQ=255)
                                                                                                                               gacgacGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTg                    >  1:3062690/1‑151 (MQ=255)
                                                                                                                                acgacgATCAGCGAGACCTCGTACTGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGACATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGc                   <  2:9262035/151‑1 (MQ=255)
                                                                                                                                acgacgAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGc                   >  1:8202920/1‑151 (MQ=255)
                                                                                                                                  gacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGctc                 >  1:3880658/1‑151 (MQ=255)
                                                                                                                                   acgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCCGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGctct                >  2:8585036/1‑151 (MQ=255)
                                                                                                                                   acgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGctct                >  2:1976576/1‑151 (MQ=255)
                                                                                                                                   acgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGctct                <  2:4565033/151‑1 (MQ=255)
                                                                                                                                    cgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCtt               <  1:6539334/151‑1 (MQ=255)
                                                                                                                                     gaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTc              <  2:10000958/151‑1 (MQ=255)
                                                                                                                                       aCAGCGAGACCACGAACTGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg            <  2:8232886/151‑1 (MQ=255)
                                                                                                                                       aCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAg            <  1:8519255/151‑1 (MQ=255)
                                                                                                                                        cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAgg           <  1:7262560/151‑1 (MQ=255)
                                                                                                                                        cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAgg           >  1:7123534/1‑151 (MQ=255)
                                                                                                                                         aGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGc          <  1:1897882/151‑1 (MQ=255)
                                                                                                                                           cGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg        >  1:1391007/1‑151 (MQ=255)
                                                                                                                                           cGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg        <  1:243187/151‑1 (MQ=255)
                                                                                                                                             agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAc      <  2:1042033/151‑1 (MQ=255)
                                                                                                                                             agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAc      >  2:7732448/1‑151 (MQ=255)
                                                                                                                                             agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAc      >  2:7441996/1‑151 (MQ=255)
                                                                                                                                             agaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAc      >  2:180165/1‑151 (MQ=255)
                                                                                                                                              gaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGAcc     <  2:8160742/151‑1 (MQ=255)
                                                                                                                                               aCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCa    <  2:3559047/151‑1 (MQ=255)
                                                                                                                                                ccACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAg   <  2:4322302/151‑1 (MQ=255)
                                                                                                                                                ccACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAg   >  1:1762064/1‑151 (MQ=255)
                                                                                                                                                ccACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAg   <  1:8856026/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑