Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,880,8720CG100.0% 2029.9 / NA 502A502G (GCC→GGC) PA14_RS08740DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (263/239);  total (263/239)

Only 100 of 508 total aligned reads displayed.

ATCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGGG  >  NC_008463/1880724‑1881019
                                                                                                                                                    |                                                                                                                                                   
   cgcgCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                                <  2:9147685/151‑1 (MQ=255)
       ccGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCt                                                                                                                                            >  1:8807166/1‑151 (MQ=255)
        cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           <  1:10467751/151‑1 (MQ=255)
        cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           >  1:8589390/1‑151 (MQ=255)
        cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTg                                                                                                                                           <  1:5651104/151‑1 (MQ=255)
         gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                          <  2:9737564/151‑1 (MQ=255)
         gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                          >  2:6614229/1‑151 (MQ=255)
         gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                          >  2:2817146/1‑151 (MQ=255)
         gAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGa                                                                                                                                          >  1:4128631/1‑151 (MQ=255)
           gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                        <  1:2042926/151‑1 (MQ=255)
           gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                        <  2:2994442/151‑1 (MQ=255)
           gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                        <  1:4587906/151‑1 (MQ=255)
             ccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgc                                                                                                                                      >  1:6950692/1‑151 (MQ=255)
             ccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgc                                                                                                                                      >  1:3754892/1‑151 (MQ=255)
              cTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCGGTGGCCGCCGTGGCGCGCGGCTGACcgcg                                                                                                                                     >  1:1187540/1‑151 (MQ=255)
              cTACGCCGGGCTGGAGCGCGAGCCCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGAGGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgcg                                                                                                                                     <  2:6024877/151‑1 (MQ=255)
                 cGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTg                                                                                                                                  >  1:2389404/1‑151 (MQ=255)
                     gggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCt                                                                                                                              >  1:7392068/1‑151 (MQ=255)
                     gggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCt                                                                                                                              <  1:8472728/151‑1 (MQ=255)
                       gCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcg                                                                                                                            <  2:9599639/151‑1 (MQ=255)
                        cTGGAGCGCGAGCGCTACGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgg                                                                                                                           <  2:7460232/151‑1 (MQ=255)
                         tGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                          >  1:3995083/1‑151 (MQ=255)
                         tGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                          <  2:2103128/151‑1 (MQ=255)
                          ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                         >  2:8596366/1‑151 (MQ=255)
                            agcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggcgg                                                                                                                       >  1:7243646/1‑151 (MQ=255)
                             gcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggcggg                                                                                                                      >  1:7934258/1‑151 (MQ=255)
                                cgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGAcc                                                                                                                   >  1:4330388/1‑151 (MQ=255)
                                   gcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAtt                                                                                                                >  2:182254/1‑151 (MQ=255)
                                    cgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                               >  2:2300658/1‑151 (MQ=255)
                                     gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTcc                                                                                                              >  1:1686414/1‑151 (MQ=255)
                                         cgacgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAt                                                                                                          <  1:9555379/151‑1 (MQ=255)
                                         cgacgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAt                                                                                                          <  1:7767235/151‑1 (MQ=255)
                                           acgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                        <  1:2966827/151‑1 (MQ=255)
                                              aaGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                     <  1:7533775/151‑1 (MQ=255)
                                              aaGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                     <  1:9987050/151‑1 (MQ=255)
                                                gTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCg                                                                                                   >  1:2010141/1‑151 (MQ=255)
                                                gTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCg                                                                                                   <  2:1319588/151‑1 (MQ=255)
                                                   cTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACt                                                                                                >  1:6286083/1‑151 (MQ=255)
                                                        gATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGc                                                                                           <  1:9900415/151‑1 (MQ=255)
                                                          tGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGt                                                                                         <  1:5164129/151‑1 (MQ=255)
                                                             tCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgct                                                                                      >  1:2787148/1‑151 (MQ=255)
                                                               gCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                    >  2:3691028/1‑151 (MQ=255)
                                                               gCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                    >  2:7839562/1‑151 (MQ=255)
                                                                ccGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgcg                                                                                   >  2:760738/1‑150 (MQ=255)
                                                                  gAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTgg                                                                                 >  1:9465785/1‑151 (MQ=255)
                                                                    gggCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAg                                                                               <  2:4600722/151‑1 (MQ=255)
                                                                      gCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGcc                                                                             <  1:556202/151‑1 (MQ=255)
                                                                        tACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGc                                                                           <  2:5497961/151‑1 (MQ=255)
                                                                            aCTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAGTCCCGAGACCGGCGACTACAGCGTGCTGCTGGAGCCGCAggg                                                                       <  1:1031994/151‑1 (MQ=255)
                                                                               cACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                    <  2:8035876/151‑1 (MQ=255)
                                                                               cACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                    <  1:1457472/151‑1 (MQ=255)
                                                                                   ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgctg                                                                >  1:4820981/1‑151 (MQ=255)
                                                                                   ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGCCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgctg                                                                >  1:6381764/1‑151 (MQ=255)
                                                                                      cGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggtg                                                             >  1:615098/1‑151 (MQ=255)
                                                                                       ggCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtggtgg                                                            >  1:8346691/1‑151 (MQ=255)
                                                                                         cGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGa                                                          <  2:1540915/151‑1 (MQ=255)
                                                                                          gTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCGGCTGGAGCCGCAGGGCCTGCGGGTGGGaa                                                         >  2:5923717/1‑151 (MQ=255)
                                                                                                ggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                   <  2:10528898/151‑1 (MQ=255)
                                                                                                ggcgCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                   >  2:2593085/1‑151 (MQ=255)
                                                                                                   gcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                                <  2:8447119/151‑1 (MQ=255)
                                                                                                    ccTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGa                                               >  1:6623580/1‑151 (MQ=255)
                                                                                                      tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGACCGGTCAACGAgg                                             >  2:5834268/1‑151 (MQ=255)
                                                                                                      tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAgg                                             >  1:9219014/1‑151 (MQ=255)
                                                                                                         ctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACt                                          <  2:3229507/151‑1 (MQ=255)
                                                                                                          tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACtt                                         >  2:5092373/1‑151 (MQ=255)
                                                                                                            cATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcg                                       >  1:1076779/1‑151 (MQ=255)
                                                                                                             aTCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                      <  1:3428018/151‑1 (MQ=255)
                                                                                                              tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgcg                                     <  1:1598566/151‑1 (MQ=255)
                                                                                                               cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                    >  2:1979628/1‑151 (MQ=255)
                                                                                                               cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                    >  1:5250282/1‑151 (MQ=255)
                                                                                                                 cgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTg                                  <  2:933563/151‑1 (MQ=255)
                                                                                                                   aTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGga                                <  2:4033602/151‑1 (MQ=255)
                                                                                                                   aTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGga                                >  2:4882253/1‑151 (MQ=255)
                                                                                                                   aTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGga                                >  2:7734570/1‑151 (MQ=255)
                                                                                                                    tGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgag                               <  1:3151907/151‑1 (MQ=255)
                                                                                                                    tGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgag                               <  1:8075532/151‑1 (MQ=255)
                                                                                                                      cgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                             <  2:3110411/151‑1 (MQ=255)
                                                                                                                      cgcACAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                             <  1:7323357/151‑1 (MQ=255)
                                                                                                                       gcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGc                            <  1:5820268/151‑1 (MQ=255)
                                                                                                                        cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGcc                           <  2:7088386/151‑1 (MQ=255)
                                                                                                                        cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGcc                           >  1:2219476/1‑151 (MQ=255)
                                                                                                                         tCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                          >  1:250936/1‑151 (MQ=255)
                                                                                                                           aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTgg                        >  2:3445304/1‑151 (MQ=255)
                                                                                                                           aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTgg                        >  2:8971963/1‑151 (MQ=255)
                                                                                                                             cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGcc                      <  1:1877024/151‑1 (MQ=255)
                                                                                                                             cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGcc                      <  2:6286584/151‑1 (MQ=255)
                                                                                                                              ggCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCg                     >  2:4233245/1‑151 (MQ=255)
                                                                                                                              ggCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCg                     >  1:6015079/1‑151 (MQ=255)
                                                                                                                                ccTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGc                   <  2:8297870/151‑1 (MQ=255)
                                                                                                                                 cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCg                  <  1:7148468/151‑1 (MQ=255)
                                                                                                                                  tGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGa                 >  1:9363402/1‑151 (MQ=255)
                                                                                                                                   gCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGAc                <  2:1723617/151‑1 (MQ=255)
                                                                                                                                      ccGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAGACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtg             <  2:1111985/151‑1 (MQ=255)
                                                                                                                                          ggCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAGTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTcc         <  2:9467543/151‑1 (MQ=255)
                                                                                                                                            ccgccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCaa       >  1:4440842/1‑151 (MQ=255)
                                                                                                                                             cgccgTGGCGCGCGGCTGACCGCGCTGTCCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTGCGCGGTGGAGAGCCGGGCCGGCGACGGGTGCCAAc      >  1:8172266/1‑151 (MQ=255)
                                                                                                                                              gccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCGGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt     <  2:1023320/151‑1 (MQ=255)
                                                                                                                                               ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTg    <  2:8716868/151‑1 (MQ=255)
                                                                                                                                                cgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCTACTgg   >  1:5759835/1‑151 (MQ=255)
                                                                                                                                                 gTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTggg  >  2:3422327/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGGG  >  NC_008463/1880724‑1881019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑