Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463888,0380CT99.8% 2091.7 / NA 492P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (270/221);  minor base G (0/1);  total (270/222)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463888,0390TG100.0% 1975.7 / NA 492P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (270/222);  total (270/222)

Only 100 of 492 total aligned reads displayed.

GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183
                                                                                                                                                 ||                                                                                                                                                
gAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAAt                                                                                                                                              <  2:7386593/151‑1 (MQ=255)
 aGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATg                                                                                                                                             <  1:8744840/151‑1 (MQ=255)
      ctctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGTAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                        <  2:8691403/151‑1 (MQ=255)
      ctctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                        >  1:7220551/1‑151 (MQ=255)
      ctctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGc                                                                                                                                        >  1:8097301/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       >  2:8391289/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       >  1:1181424/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       >  2:268782/1‑151 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    <  1:10175039/151‑1 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    >  1:4514299/1‑151 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    <  1:4823035/151‑1 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    >  1:7785798/1‑151 (MQ=255)
           cTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGc                                                                                                                                   >  1:7694786/1‑151 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGATACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  <  1:2087833/151‑1 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  >  1:962413/1‑151 (MQ=255)
             tCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTa                                                                                                                                 <  2:5783257/151‑1 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               >  2:7883485/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              <  1:6597104/151‑1 (MQ=255)
                 ccTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                             >  2:9800519/1‑151 (MQ=255)
                  cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                            >  1:2324130/1‑151 (MQ=255)
                   tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                           >  1:10333084/1‑151 (MQ=255)
                   tCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                           >  2:8076123/1‑151 (MQ=255)
                   tCGGCGGCGCCCGCGTCGACCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTg                                                                                                                           <  2:5670723/151‑1 (MQ=255)
                    cggcggCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTgg                                                                                                                          >  2:7571898/1‑151 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         <  2:6503981/151‑1 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         >  1:5334747/1‑151 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         >  1:5685201/1‑151 (MQ=255)
                      gcggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGcc                                                                                                                        >  2:241117/1‑151 (MQ=255)
                       cggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCg                                                                                                                       >  2:6360932/1‑151 (MQ=255)
                            cccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCaa                                                                                                                  >  1:1238857/1‑151 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGCCGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAt                                                                                                                 <  2:3533139/151‑1 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAt                                                                                                                 >  1:5478540/1‑151 (MQ=255)
                              cGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATg                                                                                                                <  1:618834/151‑1 (MQ=255)
                               gCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGt                                                                                                               >  1:3595831/1‑151 (MQ=255)
                                cTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTc                                                                                                              >  1:1485574/1‑151 (MQ=255)
                                     aaCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCa                                                                                                         <  2:289719/151‑1 (MQ=255)
                                        tGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGtt                                                                                                      <  1:751754/151‑1 (MQ=255)
                                            gccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCgg                                                                                                  <  1:10084168/151‑1 (MQ=255)
                                                cTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGATCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtg                                                                                              <  1:10581107/151‑1 (MQ=255)
                                                 tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgt                                                                                             >  2:2301696/1‑151 (MQ=255)
                                                  aCGCGAACGCAGCGACCGGCTGGCGCTGCTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgtg                                                                                            <  1:8485472/151‑1 (MQ=255)
                                                  aCGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgtg                                                                                            >  2:9588489/1‑151 (MQ=255)
                                                   cgcgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGATACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATgtgt                                                                                           <  1:2464492/151‑1 (MQ=255)
                                                      gAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgc                                                                                        >  2:4658018/1‑151 (MQ=255)
                                                       aaCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgcg                                                                                       <  1:4393122/151‑1 (MQ=255)
                                                        aCGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTgcgc                                                                                      <  2:5129163/151‑1 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    >  2:1459393/1‑151 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    <  2:5895256/151‑1 (MQ=255)
                                                           cAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTAc                                                                                   <  2:9066234/151‑1 (MQ=255)
                                                              cGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGcc                                                                                >  2:1319915/1‑151 (MQ=255)
                                                                aCCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTg                                                                              <  1:4203077/151‑1 (MQ=255)
                                                                 ccGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAGGTGTTGCGCTACGCCTgc                                                                             >  2:5578462/1‑151 (MQ=255)
                                                                   ggCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcgc                                                                           >  1:3648003/1‑151 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        >  2:7722926/1‑151 (MQ=255)
                                                                        gcgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAGGTGTTGCGCTACGCCTGCGCGCtgt                                                                      >  1:6417791/1‑151 (MQ=255)
                                                                          gcTGATCCGGCGCTTCCTCGACGAGGAAACTGCGCACTGCGGCGTACGCCTCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTCTCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGGGGGGCGCTACGCCTGCGCGCTGTgc                                                                    >  1:4885158/1‑151 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 >  2:8901400/1‑151 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 <  1:1711782/151‑1 (MQ=255)
                                                                             gATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCGGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggc                                                                 <  2:4857704/151‑1 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                <  2:8442610/151‑1 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                >  2:709473/1‑151 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                >  2:2164279/1‑151 (MQ=255)
                                                                                ccGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                              >  1:9139039/1‑151 (MQ=255)
                                                                                ccGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                              >  2:2951505/1‑151 (MQ=255)
                                                                                     gcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAc                                                         >  2:10241858/1‑151 (MQ=255)
                                                                                      cATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAcc                                                        <  2:10717789/151‑1 (MQ=255)
                                                                                       aTCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCAccc                                                       >  1:9447737/1‑151 (MQ=255)
                                                                                              acgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACt                                                >  2:7294848/1‑151 (MQ=255)
                                                                                               cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTc                                               >  1:2561864/1‑151 (MQ=255)
                                                                                                 aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGc                                             >  2:2629456/1‑151 (MQ=255)
                                                                                                 aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGc                                             >  1:9855853/1‑151 (MQ=255)
                                                                                                 aGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGc                                             >  2:5377615/1‑151 (MQ=255)
                                                                                                   gAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCg                                           <  2:4117876/151‑1 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          <  2:3821298/151‑1 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          <  2:2815187/151‑1 (MQ=255)
                                                                                                        cgcgCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCtt                                      >  2:1148518/1‑151 (MQ=255)
                                                                                                        cgcgCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCtt                                      >  2:1676348/1‑151 (MQ=255)
                                                                                                         gcgcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTc                                     >  1:2794753/1‑151 (MQ=255)
                                                                                                           gcACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCg                                   <  2:6706586/151‑1 (MQ=255)
                                                                                                            cACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCgg                                  <  2:10351421/151‑1 (MQ=255)
                                                                                                              cTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCGTCCGGcc                                >  1:5921315/1‑150 (MQ=255)
                                                                                                                gcggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCaga                              >  2:4397257/1‑151 (MQ=255)
                                                                                                                 cggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCagag                             <  2:8209541/151‑1 (MQ=255)
                                                                                                                 cggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCATCCGGCagag                             >  2:1578030/1‑151 (MQ=255)
                                                                                                                     gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  1:10665477/1‑151 (MQ=255)
                                                                                                                      tACGCATCGCACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGGAATGTCCGCCAGTTGCGGCCTGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCg                        <  1:3223705/151‑1 (MQ=255)
                                                                                                                      tACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCg                        <  2:6906480/151‑1 (MQ=255)
                                                                                                                        cGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTg                      <  2:3728085/151‑1 (MQ=255)
                                                                                                                           aTCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCg                   <  1:1958755/151‑1 (MQ=255)
                                                                                                                             cGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGaa                 <  1:516792/151‑1 (MQ=255)
                                                                                                                              gAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAg                >  2:3344848/1‑151 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              <  2:3118092/151‑1 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAGGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              >  1:8310943/1‑151 (MQ=255)
                                                                                                                                 cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc             <  2:6043198/151‑1 (MQ=255)
                                                                                                                                   cGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCgg           <  1:4012909/151‑1 (MQ=255)
                                                                                                                                    ggCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGa          >  1:6854860/1‑151 (MQ=255)
                                                                                                                                      cGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGaca        >  1:7983606/1‑151 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTACCGGCAGAGCTGCGTGGCGACGGCCGGacac       >  2:1411086/1‑151 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGGTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacac       <  2:1939277/151‑1 (MQ=255)
                                                                                                                                        aGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACAcc      <  2:8800001/151‑1 (MQ=255)
                                                                                                                                                 ||                                                                                                                                                
GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑